Rh7CG251400

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7C
OG0001544
Physical Location & Seq
Forward (+)
24105390 .. 24113595
8206 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7CG251400.1

Sequence Viewer

Length: 981 bp
ATGGAGTCTAGGATGGATCAGTATGAGCTGATGGAGCAGATCGGCCGGGGAGCTTTCGGAGCCGCGATTTTGGTTCATCACAAGGCTGAGAAGAAGAAGTATGTGTTGAAGAAGATCTGGCTTGCGAGGCAGACGGAGCGGTGCCGGAGATCTGCTCATCAAGAGATGGCTCTTATTGCCCGGATTCAGCACCCGTACATTGTGGAATTTAAGGAAGCCTGGGTTGAGAAGGTGAGGCACTTTTGTTCATGTGTGTATGTGGATGCGTATAAATCAGATTTGTATCTTATTGGTTTGGCTTTTAGATTTTTGGATGACATGGTTCAGGCATATGCATTAAATGGAAGAGGATTAGTTGGGGGAAGTTTCCAACTTGATTACCCTAGTGTTGGGGCAACAGAAACCCTTCTAATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAGTGGGGCCTGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTACCAAGTTTCCTGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTCCAACCGCAAACAATGGAACTGCTAACCACTTGGTTTAAGTCCTGTAGAAGTCTCTATTTGAAAAGCGCATGGCTCATGTCTGTGCTCTGCTCTGGTTTCGGGAAAGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAGCTGCATTGGCTGCAGAAGGTACTACTGAGATCAGCGGTGTTGCTCATATTGTGGTTATGAGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

326

Amino Acids

35.98

Weight (kDa)

8.67

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 8 - 79 5.7e-11 Protein kinase domain
EPSP_synthase PF00275 115 - 170 1.3e-09 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
EPSP_synthase PF00275 168 - 255 1.8e-06 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 141
AccBSI CCGCTC 1 cut(s) 139
AccII CGCG 1 cut(s) 65
AciI CCGC 5 cut(s) 63, 139, 609, 740, 948
AclWI GGATC 1 cut(s) 24
AcoI YGGCCR 2 cut(s) 43, 606
AcsI RAATTY 2 cut(s) 206, 679
AfaI GTAC 2 cut(s) 197, 934
AfiI CCNNNNNNNGG 3 cut(s) 389, 412, 716
AgsI TTSAA 3 cut(s) 109, 555, 796
AjnI CCWGG 1 cut(s) 218
AluBI AGCT 4 cut(s) 28, 53, 533, 914
AluI AGCT 4 cut(s) 28, 53, 533, 914
Alw21I GWGCWC 1 cut(s) 822
Alw26I GTCTC 2 cut(s) 669, 791
AlwI GGATC 1 cut(s) 24
Ama87I CYCGRG 1 cut(s) 716
AoxI GGCC 4 cut(s) 43, 507, 606, 707
ApeKI GCWGC 6 cut(s) 416, 527, 530, 875, 914, 923
ApoI RAATTY 2 cut(s) 206, 679
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 803
AspS9I GGNCC 2 cut(s) 507, 707
AsuC2I CCSGG 4 cut(s) 47, 181, 717, 718
AsuHPI GGTGA 3 cut(s) 244, 552, 686
AvaI CYCGRG 1 cut(s) 716
BanI GGYRCC 1 cut(s) 141
Bbv12I GWGCWC 1 cut(s) 822
BbvI GCAGC 6 cut(s) 403, 514, 542, 862, 901, 910
BccI CCATC 4 cut(s) 7, 25, 160, 425
BciT130I CCWGG 1 cut(s) 220
BcnI CCSGG 4 cut(s) 47, 181, 717, 718
BcoDI GTCTC 2 cut(s) 669, 791
BfaI CTAG 2 cut(s) 9, 384
BfmI CTRYAG 4 cut(s) 528, 726, 778, 924
BglII AGATCT 3 cut(s) 114, 149, 730
BisI GCNGC 8 cut(s) 63, 417, 528, 531, 609, 876, 915, 924
BlsI GCNGC 8 cut(s) 64, 418, 529, 532, 610, 877, 916, 925
Bme1390I CCNGG 5 cut(s) 47, 181, 220, 717, 718
BmeT110I CYCGRG 1 cut(s) 716
BmgT120I GGNCC 2 cut(s) 507, 707
BmiI GGNNCC 4 cut(s) 61, 143, 508, 865
BmrFI CCNGG 5 cut(s) 47, 181, 220, 717, 718
BmsI GCATC 2 cut(s) 253, 582
BplI GAGNNNNNCTC 2 cut(s) 139, 171
BpuMI CCSGG 4 cut(s) 47, 181, 717, 718
BsaBI GATNNNNATC 1 cut(s) 282
BsaJI CCNNGG 4 cut(s) 46, 219, 645, 716
Bsc4I CCNNNNNNNGG 3 cut(s) 389, 412, 716
Bse3DI GCAATG 1 cut(s) 635
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 1 cut(s) 220
BseDI CCNNGG 4 cut(s) 46, 219, 645, 716
BseGI GGATG 3 cut(s) 18, 268, 319
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 389, 412, 716
BseMI GCAATG 1 cut(s) 635
BseMII CTCAG 3 cut(s) 78, 901, 930
BseX3I CGGCCG 1 cut(s) 43
BseXI GCAGC 6 cut(s) 403, 514, 542, 862, 901, 910
BsgI GTGCAG 1 cut(s) 496
Bsh1236I CGCG 1 cut(s) 65
Bsh1285I CGRYCG 1 cut(s) 46
BshFI GGCC 4 cut(s) 45, 509, 608, 709
BshNI GGYRCC 1 cut(s) 141
BsiEI CGRYCG 1 cut(s) 46
BsiHKAI GWGCWC 1 cut(s) 822
BsiHKCI CYCGRG 1 cut(s) 716
BsiSI CCGG 4 cut(s) 46, 145, 181, 717
BslI CCNNNNNNNGG 3 cut(s) 389, 412, 716
BsmAI GTCTC 2 cut(s) 669, 791
BsnI GGCC 4 cut(s) 45, 509, 608, 709
BsoBI CYCGRG 1 cut(s) 716
Bsp1286I GDGCHC 1 cut(s) 822
Bsp143I GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
BspACI CCGC 5 cut(s) 63, 139, 609, 740, 948
BspANI GGCC 4 cut(s) 45, 509, 608, 709
BspCNI CTCAG 3 cut(s) 79, 900, 931
BspFNI CGCG 1 cut(s) 65
BspHI TCATGA 1 cut(s) 637
BspLI GGNNCC 4 cut(s) 61, 143, 508, 865
BspMAI CTGCAG 2 cut(s) 532, 928
BspPI GGATC 1 cut(s) 24
BspT107I GGYRCC 1 cut(s) 141
BsrBI CCGCTC 1 cut(s) 139
BsrDI GCAATG 1 cut(s) 635
BssECI CCNNGG 4 cut(s) 46, 219, 645, 716
BssMI GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
BssT1I CCWWGG 1 cut(s) 645
Bst2UI CCWGG 1 cut(s) 220
Bst4CI ACNGT 1 cut(s) 447
Bst6I CTCTTC 1 cut(s) 340
BstAPI GCANNNNNTGC 1 cut(s) 923
BstC8I GCNNGC 3 cut(s) 123, 511, 525
BstDEI CTNAG 3 cut(s) 87, 887, 939
BstF5I GGATG 3 cut(s) 18, 268, 319
BstFNI CGCG 1 cut(s) 65
BstHHI GCGC 1 cut(s) 803
BstKTI GATC 7 cut(s) 19, 42, 117, 152, 481, 733, 945
BstMAI GTCTC 2 cut(s) 669, 791
BstMBI GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
BstMCI CGRYCG 1 cut(s) 46
BstMWI GCNNNNNNNGC 8 cut(s) 34, 59, 127, 136, 176, 425, 920, 923
BstNI CCWGG 1 cut(s) 220
BstNSI RCATGY 1 cut(s) 423
BstSCI CCNGG 5 cut(s) 45, 179, 218, 715, 716
BstSFI CTRYAG 4 cut(s) 528, 726, 778, 924
BstUI CGCG 1 cut(s) 65
BstV1I GCAGC 6 cut(s) 403, 514, 542, 862, 901, 910
BstX2I RGATCY 3 cut(s) 114, 149, 730
BstYI RGATCY 3 cut(s) 114, 149, 730
BstZI CGGCCG 1 cut(s) 43
BsuRI GGCC 4 cut(s) 45, 509, 608, 709
BtsCI GGATG 3 cut(s) 18, 268, 319
BtsI GCAGTG 1 cut(s) 666
BtsIMutI CAGTG 1 cut(s) 666
Cac8I GCNNGC 3 cut(s) 123, 511, 525
CciI TCATGA 1 cut(s) 637
CfoI GCGC 1 cut(s) 803
Cfr13I GGNCC 2 cut(s) 507, 707
Cfr9I CCCGGG 1 cut(s) 716
Csp6I GTAC 2 cut(s) 196, 933
CviAII CATG 6 cut(s) 249, 319, 420, 638, 804, 811
CviQI GTAC 2 cut(s) 196, 933
DdeI CTNAG 3 cut(s) 87, 887, 939
DpnI GATC 7 cut(s) 18, 41, 116, 151, 480, 732, 944
DpnII GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
EaeI YGGCCR 2 cut(s) 43, 606
EagI CGGCCG 1 cut(s) 43
Eam1104I CTCTTC 1 cut(s) 340
EarI CTCTTC 1 cut(s) 340
EclXI CGGCCG 1 cut(s) 43
Eco130I CCWWGG 1 cut(s) 645
Eco32I GATATC 1 cut(s) 900
Eco52I CGGCCG 1 cut(s) 43
Eco88I CYCGRG 1 cut(s) 716
EcoO109I RGGNCCY 1 cut(s) 507
EcoRII CCWGG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 900
EcoT14I CCWWGG 1 cut(s) 645
EcoT22I ATGCAT 1 cut(s) 337
ErhI CCWWGG 1 cut(s) 645
FaeI CATG 6 cut(s) 252, 322, 423, 641, 807, 814
FatI CATG 6 cut(s) 248, 318, 419, 637, 803, 810
FauNDI CATATG 1 cut(s) 331
Fnu4HI GCNGC 8 cut(s) 63, 417, 528, 531, 609, 876, 915, 924
FokI GGATG 3 cut(s) 25, 275, 326
Fsp4HI GCNGC 8 cut(s) 63, 417, 528, 531, 609, 876, 915, 924
FspBI CTAG 2 cut(s) 9, 384
GlaI GCGC 1 cut(s) 802
GluI GCNGC 8 cut(s) 63, 417, 528, 531, 609, 876, 915, 924
HaeIII GGCC 4 cut(s) 45, 509, 608, 709
HapII CCGG 4 cut(s) 46, 145, 181, 717
HhaI GCGC 1 cut(s) 803
Hin1II CATG 6 cut(s) 252, 322, 423, 641, 807, 814
Hin6I GCGC 1 cut(s) 801
HinP1I GCGC 1 cut(s) 801
HinfI GANTC 2 cut(s) 5, 184
HpaII CCGG 4 cut(s) 46, 145, 181, 717
HphI GGTGA 3 cut(s) 244, 552, 686
Hpy188I TCNGA 5 cut(s) 59, 277, 495, 591, 890
Hpy188III TCNNGA 4 cut(s) 161, 638, 666, 835
HpyAV CCTTC 3 cut(s) 223, 416, 923
HpyCH4III ACNGT 1 cut(s) 447
HpyCH4V TGCA 9 cut(s) 335, 419, 513, 530, 595, 618, 878, 917, 926
HpyF10VI GCNNNNNNNGC 8 cut(s) 34, 59, 127, 136, 176, 425, 920, 923
HpyF3I CTNAG 3 cut(s) 87, 887, 939
Hsp92II CATG 6 cut(s) 252, 322, 423, 641, 807, 814
HspAI GCGC 1 cut(s) 801
Kzo9I GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
LmnI GCTCC 4 cut(s) 34, 50, 59, 136
Lsp1109I GCAGC 6 cut(s) 403, 514, 542, 862, 901, 910
LweI GCATC 2 cut(s) 253, 582
MaeI CTAG 2 cut(s) 9, 384
MalI GATC 7 cut(s) 18, 41, 116, 151, 480, 732, 944
MbiI CCGCTC 1 cut(s) 139
MboI GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
MboII GAAGA 5 cut(s) 103, 106, 121, 124, 357
MflI RGATCY 3 cut(s) 114, 149, 730
MhlI GDGCHC 1 cut(s) 822
MluCI AATT 3 cut(s) 206, 555, 679
MlyI GAGTC 1 cut(s) 14
MmeI TCCRAC 2 cut(s) 394, 760
MnlI CCTC 8 cut(s) 120, 228, 341, 463, 680, 688, 884, 896
Mph1103I ATGCAT 1 cut(s) 337
MroXI GAANNNNTTC 1 cut(s) 405
MseI TTAA 4 cut(s) 210, 338, 699, 771
MslI CAYNNNNRTG 2 cut(s) 518, 815
MspA1I CMGCKG 2 cut(s) 611, 948
MspI CCGG 4 cut(s) 46, 145, 181, 717
MspR9I CCNGG 5 cut(s) 47, 181, 220, 717, 718
MvaI CCWGG 1 cut(s) 220
MvnI CGCG 1 cut(s) 65
MwoI GCNNNNNNNGC 8 cut(s) 34, 59, 127, 136, 176, 425, 920, 923
NciI CCSGG 4 cut(s) 47, 181, 717, 718
NdeI CATATG 1 cut(s) 331
NdeII GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
NlaIII CATG 6 cut(s) 252, 322, 423, 641, 807, 814
NlaIV GGNNCC 4 cut(s) 61, 143, 508, 865
NsiI ATGCAT 1 cut(s) 337
NspI RCATGY 1 cut(s) 423
PagI TCATGA 1 cut(s) 637
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 184
PkrI GCNGC 8 cut(s) 64, 418, 529, 532, 610, 877, 916, 925
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
Psp6I CCWGG 1 cut(s) 218
PspGI CCWGG 1 cut(s) 218
PspN4I GGNNCC 4 cut(s) 61, 143, 508, 865
PspPI GGNCC 2 cut(s) 507, 707
PstI CTGCAG 2 cut(s) 532, 928
PsuI RGATCY 3 cut(s) 114, 149, 730
RsaI GTAC 2 cut(s) 197, 934
RsaNI GTAC 2 cut(s) 196, 933
RseI CAYNNNNRTG 2 cut(s) 518, 815
SaqAI TTAA 4 cut(s) 210, 338, 699, 771
SatI GCNGC 8 cut(s) 63, 417, 528, 531, 609, 876, 915, 924
Sau3AI GATC 7 cut(s) 16, 39, 114, 149, 478, 730, 942
Sau96I GGNCC 2 cut(s) 507, 707
SchI GAGTC 1 cut(s) 14
ScrFI CCNGG 5 cut(s) 47, 181, 220, 717, 718
SduI GDGCHC 1 cut(s) 822
SfaNI GCATC 2 cut(s) 253, 582
SfcI CTRYAG 4 cut(s) 528, 726, 778, 924
SmaI CCCGGG 1 cut(s) 718
SmiMI CAYNNNNRTG 2 cut(s) 518, 815
Sse9I AATT 3 cut(s) 206, 555, 679
SsiI CCGC 5 cut(s) 63, 139, 609, 740, 948
SspMI CTAG 2 cut(s) 9, 384
StyD4I CCNGG 5 cut(s) 45, 179, 218, 715, 716
StyI CCWWGG 1 cut(s) 645
TaaI ACNGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 487, 667
TasI AATT 3 cut(s) 206, 555, 679
TauI GCSGC 2 cut(s) 65, 611
TfiI GAWTC 1 cut(s) 184
Tru1I TTAA 4 cut(s) 210, 338, 699, 771
Tru9I TTAA 4 cut(s) 210, 338, 699, 771
TscAI CASTG 1 cut(s) 666
TseI GCWGC 6 cut(s) 416, 527, 530, 875, 914, 923
TspDTI ATGAA 3 cut(s) 65, 237, 538
TspGWI ACGGA 1 cut(s) 149
TspMI CCCGGG 1 cut(s) 716
TspRI CASTG 1 cut(s) 666
XapI RAATTY 2 cut(s) 206, 679
XceI RCATGY 1 cut(s) 423
XmaI CCCGGG 1 cut(s) 716
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 2 cut(s) 9, 384
Zsp2I ATGCAT 1 cut(s) 337
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.