Rh1BG113100

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0001544
Physical Location & Seq
Forward (+)
19283540 .. 19284709
1170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG113100.1

Sequence Viewer

Length: 528 bp
ATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCCTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTCCAACCGCAAACAATGGCACTGCTAGCCACTTGGTTTAAGTCCTGTAGAAGTCTGTATTTGAAAAGCGCATGGCTCATGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGCATTGGCTGCAGAAGGTGCTACTGAGATCAGCGGTGTTGCTCATATTGACCGTGGTTATGAGAATGTAGATACAAAAGTTCATATGCTGGGAGCCGATATCAAAAGATTAACGGCCCCTGCTTCTACCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

175

Amino Acids

18.54

Weight (kDa)

8.92

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 155 5.6e-12 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 90, 221, 429
AcoI YGGCCR 1 cut(s) 87
AcsI RAATTY 2 cut(s) 160, 309
AfiI CCNNNNNNNGG 1 cut(s) 197
AgsI TTSAA 3 cut(s) 36, 277, 314
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 3 cut(s) 14, 298, 327
AluI AGCT 3 cut(s) 14, 298, 327
Alw26I GTCTC 1 cut(s) 150
Ama87I CYCGRG 1 cut(s) 197
AoxI GGCC 3 cut(s) 87, 188, 510
ApeKI GCWGC 4 cut(s) 8, 11, 365, 404
ApoI RAATTY 2 cut(s) 160, 309
AspLEI GCGC 1 cut(s) 284
AspS9I GGNCC 2 cut(s) 188, 511
AsuC2I CCSGG 2 cut(s) 198, 199
AsuHPI GGTGA 2 cut(s) 33, 167
AsuNHI GCTAGC 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 197
BaeI ACNNNNGTAYC 2 cut(s) 459, 492
BbvI GCAGC 3 cut(s) 23, 352, 391
BceAI ACGGC 1 cut(s) 525
BciT130I CCWGG 1 cut(s) 86
BcnI CCSGG 2 cut(s) 198, 199
BcoDI GTCTC 1 cut(s) 150
BfaI CTAG 2 cut(s) 239, 526
BfmI CTRYAG 4 cut(s) 9, 207, 259, 405
BglII AGATCT 1 cut(s) 211
BisI GCNGC 5 cut(s) 9, 12, 90, 366, 405
BlsI GCNGC 5 cut(s) 10, 13, 91, 367, 406
Bme1390I CCNGG 3 cut(s) 86, 198, 199
BmeT110I CYCGRG 1 cut(s) 197
BmgT120I GGNCC 2 cut(s) 188, 511
BmiI GGNNCC 3 cut(s) 355, 490, 513
BmrFI CCNGG 3 cut(s) 86, 198, 199
BmsI GCATC 1 cut(s) 63
BmtI GCTAGC 1 cut(s) 242
BpuMI CCSGG 2 cut(s) 198, 199
BsaJI CCNNGG 3 cut(s) 126, 197, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 197
Bse3DI GCAATG 1 cut(s) 116
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 3 cut(s) 126, 197, 448
BseLI CCNNNNNNNGG 1 cut(s) 197
BseMI GCAATG 1 cut(s) 116
BseMII CTCAG 2 cut(s) 391, 411
BseXI GCAGC 3 cut(s) 23, 352, 391
BseYI CCCAGC 1 cut(s) 484
BshFI GGCC 3 cut(s) 89, 190, 512
BsiHKCI CYCGRG 1 cut(s) 197
BsiSI CCGG 1 cut(s) 198
BslI CCNNNNNNNGG 1 cut(s) 197
BsmAI GTCTC 1 cut(s) 150
BsnI GGCC 3 cut(s) 89, 190, 512
BsoBI CYCGRG 1 cut(s) 197
Bsp143I GATC 2 cut(s) 211, 423
BspACI CCGC 3 cut(s) 90, 221, 429
BspANI GGCC 3 cut(s) 89, 190, 512
BspCNI CTCAG 2 cut(s) 390, 412
BspHI TCATGA 2 cut(s) 118, 291
BspLI GGNNCC 3 cut(s) 355, 490, 513
BspMAI CTGCAG 2 cut(s) 13, 409
BspOI GCTAGC 1 cut(s) 242
BsrDI GCAATG 1 cut(s) 116
BssECI CCNNGG 3 cut(s) 126, 197, 448
BssMI GATC 2 cut(s) 211, 423
BssT1I CCWWGG 1 cut(s) 126
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 449
BstAPI GCANNNNNTGC 2 cut(s) 404, 413
BstC8I GCNNGC 3 cut(s) 6, 240, 325
BstDEI CTNAG 2 cut(s) 377, 420
BstDSI CCRYGG 1 cut(s) 448
BstHHI GCGC 1 cut(s) 284
BstKTI GATC 2 cut(s) 214, 426
BstMAI GTCTC 1 cut(s) 150
BstMBI GATC 2 cut(s) 211, 423
BstMWI GCNNNNNNNGC 4 cut(s) 239, 295, 404, 413
BstNI CCWGG 1 cut(s) 86
BstSCI CCNGG 3 cut(s) 84, 196, 197
BstSFI CTRYAG 4 cut(s) 9, 207, 259, 405
BstV1I GCAGC 3 cut(s) 23, 352, 391
BstX2I RGATCY 1 cut(s) 211
BstYI RGATCY 1 cut(s) 211
BsuRI GGCC 3 cut(s) 89, 190, 512
BtgI CCRYGG 1 cut(s) 448
BtsI GCAGTG 2 cut(s) 147, 233
BtsIMutI CAGTG 2 cut(s) 147, 233
Cac8I GCNNGC 3 cut(s) 6, 240, 325
CciI TCATGA 2 cut(s) 118, 291
CfoI GCGC 1 cut(s) 284
Cfr13I GGNCC 2 cut(s) 188, 511
Cfr9I CCCGGG 1 cut(s) 197
CviAII CATG 3 cut(s) 119, 285, 292
DdeI CTNAG 2 cut(s) 377, 420
DpnI GATC 2 cut(s) 213, 425
DpnII GATC 2 cut(s) 211, 423
EaeI YGGCCR 1 cut(s) 87
Eco130I CCWWGG 1 cut(s) 126
Eco32I GATATC 2 cut(s) 390, 496
Eco88I CYCGRG 1 cut(s) 197
EcoRII CCWGG 1 cut(s) 84
EcoRV GATATC 2 cut(s) 390, 496
EcoT14I CCWWGG 1 cut(s) 126
ErhI CCWWGG 1 cut(s) 126
FaeI CATG 3 cut(s) 122, 288, 295
FaiI YATR 7 cut(s) 120, 286, 293, 441, 456, 480, 482
FatI CATG 3 cut(s) 118, 284, 291
FauNDI CATATG 1 cut(s) 480
Fnu4HI GCNGC 5 cut(s) 9, 12, 90, 366, 405
Fsp4HI GCNGC 5 cut(s) 9, 12, 90, 366, 405
FspBI CTAG 2 cut(s) 239, 526
GlaI GCGC 1 cut(s) 283
GluI GCNGC 5 cut(s) 9, 12, 90, 366, 405
GsaI CCCAGC 1 cut(s) 488
HaeIII GGCC 3 cut(s) 89, 190, 512
HapII CCGG 1 cut(s) 198
HhaI GCGC 1 cut(s) 284
Hin1II CATG 3 cut(s) 122, 288, 295
Hin6I GCGC 1 cut(s) 282
HinP1I GCGC 1 cut(s) 282
HindIII AAGCTT 1 cut(s) 296
HpaII CCGG 1 cut(s) 198
HphI GGTGA 2 cut(s) 33, 167
Hpy188I TCNGA 3 cut(s) 72, 331, 380
Hpy188III TCNNGA 3 cut(s) 119, 147, 292
HpyAV CCTTC 2 cut(s) 63, 404
HpyCH4III ACNGT 1 cut(s) 449
HpyCH4V TGCA 6 cut(s) 11, 76, 99, 323, 368, 407
HpyF10VI GCNNNNNNNGC 4 cut(s) 239, 295, 404, 413
HpyF3I CTNAG 2 cut(s) 377, 420
Hsp92II CATG 3 cut(s) 122, 288, 295
HspAI GCGC 1 cut(s) 282
Kzo9I GATC 2 cut(s) 211, 423
LmnI GCTCC 2 cut(s) 302, 488
Lsp1109I GCAGC 3 cut(s) 23, 352, 391
LweI GCATC 1 cut(s) 63
MaeI CTAG 2 cut(s) 239, 526
MalI GATC 2 cut(s) 213, 425
MboI GATC 2 cut(s) 211, 423
MflI RGATCY 1 cut(s) 211
MluCI AATT 3 cut(s) 36, 160, 309
MmeI TCCRAC 1 cut(s) 241
MnlI CCTC 4 cut(s) 161, 169, 374, 386
MseI TTAA 3 cut(s) 180, 252, 506
MspA1I CMGCKG 2 cut(s) 92, 429
MspI CCGG 1 cut(s) 198
MspR9I CCNGG 3 cut(s) 86, 198, 199
MvaI CCWGG 1 cut(s) 86
MwoI GCNNNNNNNGC 4 cut(s) 239, 295, 404, 413
NciI CCSGG 2 cut(s) 198, 199
NdeI CATATG 1 cut(s) 480
NdeII GATC 2 cut(s) 211, 423
NheI GCTAGC 1 cut(s) 238
NlaIII CATG 3 cut(s) 122, 288, 295
NlaIV GGNNCC 3 cut(s) 355, 490, 513
PagI TCATGA 2 cut(s) 118, 291
PkrI GCNGC 5 cut(s) 10, 13, 91, 367, 406
Psp6I CCWGG 1 cut(s) 84
PspFI CCCAGC 1 cut(s) 484
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 3 cut(s) 355, 490, 513
PspPI GGNCC 2 cut(s) 188, 511
PstI CTGCAG 2 cut(s) 13, 409
PsuI RGATCY 1 cut(s) 211
SaqAI TTAA 3 cut(s) 180, 252, 506
SatI GCNGC 5 cut(s) 9, 12, 90, 366, 405
Sau3AI GATC 2 cut(s) 211, 423
Sau96I GGNCC 2 cut(s) 188, 511
ScrFI CCNGG 3 cut(s) 86, 198, 199
SfaNI GCATC 1 cut(s) 63
SfcI CTRYAG 4 cut(s) 9, 207, 259, 405
SmaI CCCGGG 1 cut(s) 199
Sse9I AATT 3 cut(s) 36, 160, 309
SsiI CCGC 3 cut(s) 90, 221, 429
SspMI CTAG 2 cut(s) 239, 526
StyD4I CCNGG 3 cut(s) 84, 196, 197
StyI CCWWGG 1 cut(s) 126
TaaI ACNGT 1 cut(s) 449
TaqI TCGA 1 cut(s) 148
TasI AATT 3 cut(s) 36, 160, 309
TauI GCSGC 1 cut(s) 92
Tru1I TTAA 3 cut(s) 180, 252, 506
Tru9I TTAA 3 cut(s) 180, 252, 506
TscAI CASTG 2 cut(s) 147, 240
TseI GCWGC 4 cut(s) 8, 11, 365, 404
TspDTI ATGAA 3 cut(s) 19, 308, 467
TspMI CCCGGG 1 cut(s) 197
TspRI CASTG 2 cut(s) 147, 240
XapI RAATTY 2 cut(s) 160, 309
XmaI CCCGGG 1 cut(s) 197
XspI CTAG 2 cut(s) 239, 526
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.