Rh5DG281900

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001544
Physical Location & Seq
Forward (+)
34701415 .. 34702793
1379 bp
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UTR
Exon/CDS
Intron
Rh5DG281900.1

Sequence Viewer

Length: 636 bp
ATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTTGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAAAAATGGGGCCTGTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTTATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCGGTCTGATGCAAAAACTCTTGGCCGCTGGTTGCAAAATAAGGCAATGCTCTCATGATACCTTGGAAGTTTCAGCAGTGTCTCAATGTGGTGAAAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTTCAACTGCAAACAATGGCACTGCTAACCACTTGGTTTAAGTCCTGTAGAAGTCTATTTGAAAAGCGCATGGCTCATGTAAACGAACTGCAGAAGCTTGGAGCAAAAATTCAAGTCTGTGCAAGCTCTGCTCTGGTTTCAGGAAAGATAATAGAAATGGTTTGTCTGGTTCCTGCCTTGCTGCAACTGACCTCAGAGGTGGGATATCATTGGTATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

211

Amino Acids

22.71

Weight (kDa)

8.69

Isoelectric Point (pI)

41.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 179 1.6e-32 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 294
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 2 cut(s) 364, 525
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 5 cut(s) 191, 240, 422, 479, 530
AluBI AGCT 4 cut(s) 127, 218, 514, 543
AluI AGCT 4 cut(s) 127, 218, 514, 543
Alw26I GTCTC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 3 cut(s) 96, 291, 392
ApeKI GCWGC 4 cut(s) 5, 212, 215, 598
ApoI RAATTY 2 cut(s) 364, 525
AspLEI GCGC 1 cut(s) 486
AspS9I GGNCC 2 cut(s) 96, 392
AsuC2I CCSGG 2 cut(s) 402, 403
AsuHPI GGTGA 2 cut(s) 237, 371
AvaI CYCGRG 1 cut(s) 401
BbvI GCAGC 3 cut(s) 199, 227, 585
BccI CCATC 1 cut(s) 14
BcnI CCSGG 2 cut(s) 402, 403
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 4 cut(s) 213, 411, 463, 506
BglII AGATCT 1 cut(s) 415
BisI GCNGC 5 cut(s) 6, 213, 216, 294, 599
BlsI GCNGC 5 cut(s) 7, 214, 217, 295, 600
Bme1390I CCNGG 2 cut(s) 402, 403
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 96, 392
BmiI GGNNCC 2 cut(s) 97, 588
BmrFI CCNGG 2 cut(s) 402, 403
BmsI GCATC 1 cut(s) 267
BpuMI CCSGG 2 cut(s) 402, 403
BsaJI CCNNGG 2 cut(s) 330, 401
BsaWI WCCGGW 1 cut(s) 269
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 320
BseDI CCNNGG 2 cut(s) 330, 401
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 320
BseMII CTCAG 1 cut(s) 624
BseXI GCAGC 3 cut(s) 199, 227, 585
BshFI GGCC 3 cut(s) 98, 293, 394
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 2 cut(s) 270, 402
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 354
BsnI GGCC 3 cut(s) 98, 293, 394
BsoBI CYCGRG 1 cut(s) 401
Bsp143I GATC 3 cut(s) 67, 181, 415
BspACI CCGC 1 cut(s) 294
BspANI GGCC 3 cut(s) 98, 293, 394
BspCNI CTCAG 1 cut(s) 623
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 2 cut(s) 97, 588
BspMAI CTGCAG 2 cut(s) 217, 510
BsrDI GCAATG 1 cut(s) 320
BssECI CCNNGG 2 cut(s) 330, 401
BssMI GATC 3 cut(s) 67, 181, 415
BssT1I CCWWGG 1 cut(s) 330
Bst4CI ACNGT 1 cut(s) 36
BstAPI GCANNNNNTGC 1 cut(s) 545
BstC8I GCNNGC 3 cut(s) 196, 210, 541
BstDEI CTNAG 1 cut(s) 610
BstHHI GCGC 1 cut(s) 486
BstKTI GATC 3 cut(s) 70, 184, 418
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 3 cut(s) 67, 181, 415
BstMWI GCNNNNNNNGC 2 cut(s) 14, 545
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 400, 401
BstSFI CTRYAG 4 cut(s) 213, 411, 463, 506
BstV1I GCAGC 3 cut(s) 199, 227, 585
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 3 cut(s) 98, 293, 394
BtsI GCAGTG 2 cut(s) 351, 437
BtsIMutI CAGTG 2 cut(s) 351, 437
Cac8I GCNNGC 3 cut(s) 196, 210, 541
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 486
Cfr13I GGNCC 2 cut(s) 96, 392
Cfr9I CCCGGG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 168
CviAII CATG 5 cut(s) 9, 155, 323, 487, 494
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 1 cut(s) 610
DpnI GATC 3 cut(s) 69, 183, 417
DpnII GATC 3 cut(s) 67, 181, 415
EaeI YGGCCR 1 cut(s) 291
Eco130I CCWWGG 1 cut(s) 330
Eco32I GATATC 1 cut(s) 623
Eco88I CYCGRG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 96
EcoRV GATATC 1 cut(s) 623
EcoT14I CCWWGG 1 cut(s) 330
ErhI CCWWGG 1 cut(s) 330
FaeI CATG 5 cut(s) 12, 158, 326, 490, 497
FaiI YATR 8 cut(s) 10, 14, 32, 156, 206, 324, 488, 495
FatI CATG 5 cut(s) 8, 154, 322, 486, 493
Fnu4HI GCNGC 5 cut(s) 6, 213, 216, 294, 599
Fsp4HI GCNGC 5 cut(s) 6, 213, 216, 294, 599
GlaI GCGC 1 cut(s) 485
GluI GCNGC 5 cut(s) 6, 213, 216, 294, 599
HaeIII GGCC 3 cut(s) 98, 293, 394
HapII CCGG 2 cut(s) 270, 402
HhaI GCGC 1 cut(s) 486
Hin1II CATG 5 cut(s) 12, 158, 326, 490, 497
Hin6I GCGC 1 cut(s) 484
HinP1I GCGC 1 cut(s) 484
HindIII AAGCTT 2 cut(s) 125, 512
HpaII CCGG 2 cut(s) 270, 402
HphI GGTGA 2 cut(s) 237, 371
Hpy166II GTNNAC 1 cut(s) 499
Hpy188I TCNGA 3 cut(s) 84, 276, 613
Hpy188III TCNNGA 2 cut(s) 323, 558
Hpy8I GTNNAC 1 cut(s) 499
HpyAV CCTTC 2 cut(s) 101, 267
HpyCH4III ACNGT 1 cut(s) 36
HpyF10VI GCNNNNNNNGC 2 cut(s) 14, 545
HpyF3I CTNAG 1 cut(s) 610
Hsp92II CATG 5 cut(s) 12, 158, 326, 490, 497
HspAI GCGC 1 cut(s) 484
Kzo9I GATC 3 cut(s) 67, 181, 415
LmnI GCTCC 2 cut(s) 110, 518
Lsp1109I GCAGC 3 cut(s) 199, 227, 585
LweI GCATC 1 cut(s) 267
MalI GATC 3 cut(s) 69, 183, 417
MboI GATC 3 cut(s) 67, 181, 415
MboII GAAGA 1 cut(s) 410
MflI RGATCY 1 cut(s) 415
MluCI AATT 3 cut(s) 240, 364, 525
MnlI CCTC 5 cut(s) 52, 365, 373, 607, 619
MseI TTAA 2 cut(s) 384, 456
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 2 cut(s) 270, 402
MspR9I CCNGG 2 cut(s) 402, 403
MwoI GCNNNNNNNGC 2 cut(s) 14, 545
NciI CCSGG 2 cut(s) 402, 403
NdeII GATC 3 cut(s) 67, 181, 415
NlaIII CATG 5 cut(s) 12, 158, 326, 490, 497
NlaIV GGNNCC 2 cut(s) 97, 588
NspI RCATGY 1 cut(s) 12
PagI TCATGA 1 cut(s) 322
PkrI GCNGC 5 cut(s) 7, 214, 217, 295, 600
PspN4I GGNNCC 2 cut(s) 97, 588
PspPI GGNCC 2 cut(s) 96, 392
PstI CTGCAG 2 cut(s) 217, 510
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 2 cut(s) 384, 456
SatI GCNGC 5 cut(s) 6, 213, 216, 294, 599
Sau3AI GATC 3 cut(s) 67, 181, 415
Sau96I GGNCC 2 cut(s) 96, 392
ScrFI CCNGG 2 cut(s) 402, 403
SfaNI GCATC 1 cut(s) 267
SfcI CTRYAG 4 cut(s) 213, 411, 463, 506
SmaI CCCGGG 1 cut(s) 403
SmiMI CAYNNNNRTG 1 cut(s) 203
Sse9I AATT 3 cut(s) 240, 364, 525
SsiI CCGC 1 cut(s) 294
StyD4I CCNGG 2 cut(s) 400, 401
StyI CCWWGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 76
TasI AATT 3 cut(s) 240, 364, 525
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
Tru1I TTAA 2 cut(s) 384, 456
Tru9I TTAA 2 cut(s) 384, 456
TscAI CASTG 2 cut(s) 351, 444
TseI GCWGC 4 cut(s) 5, 212, 215, 598
TspDTI ATGAA 1 cut(s) 223
TspMI CCCGGG 1 cut(s) 401
TspRI CASTG 2 cut(s) 351, 444
XapI RAATTY 2 cut(s) 364, 525
XceI RCATGY 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.