Rh5DG502600

EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001544
Physical Location & Seq
Reverse (-)
78693741 .. 78697086
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG502600.1

Sequence Viewer

Length: 576 bp
ATGGCTGCATGTATGGCTGATGGAACAACTATACTGTCCAATGTGGCCAGAGAACCAGAGGTGGTTGATCTTGCTCGATTTCTGACTAACAGTGGGGCCTTTGTGGAAGGAGCAGGAAGCAACAAGCTTGTCATCAAGGGAAAATCTCATTTGCATGGTTGTAAATGTACTATTGCACCTGATCGTATTGAAGCAGGCACATTTATGCTTGCTGCAGCTATTACTCGCTCATTCATTTCAATTTCACCTGTCATTCCTTCCCAAGTTTCCTGTCTGATGCAAAAACTCTTAGCCGCGGGTTGCAAAATAAGGCAATGCTGTCATGATACCGTGGAAGTGTCAGCAGTGTCTCGATGTGGTGAGAATTTGCGAGGTTTTGAGGTTAAGACAGGGCCATTTCCCGGGTTTCCTACAGATCTTCAACCGCAAACAATGGCACTGCTAAGCACTTGGTTTAAGTCCTGTAGAAGAGTGTATTTGAAAAGCGCATGGCTCATGGTTTGTGGTATATACTGTGTGCATCCGCATTTGTATTATATATGCAGATTCTCAATCAATGAAGGTGTGGATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPSP_synthase

Protein Analysis

191

Amino Acids

20.82

Weight (kDa)

8.62

Isoelectric Point (pI)

38.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPSP_synthase PF00275 1 - 149 1.9e-27 EPSP synthase (3-phosphoshikimate 1-carboxyvinyltransferase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 296
AciI CCGC 4 cut(s) 294, 296, 425, 524
AcoI YGGCCR 1 cut(s) 45
AcsI RAATTY 1 cut(s) 364
AfaI GTAC 1 cut(s) 169
AfiI CCNNNNNNNGG 1 cut(s) 401
AgsI TTSAA 4 cut(s) 191, 240, 422, 481
AluBI AGCT 2 cut(s) 127, 218
AluI AGCT 2 cut(s) 127, 218
Alw26I GTCTC 1 cut(s) 354
Ama87I CYCGRG 1 cut(s) 401
AoxI GGCC 3 cut(s) 45, 96, 392
ApeKI GCWGC 3 cut(s) 5, 212, 215
ApoI RAATTY 1 cut(s) 364
AspLEI GCGC 1 cut(s) 488
AspS9I GGNCC 2 cut(s) 96, 392
AsuC2I CCSGG 2 cut(s) 402, 403
AsuHPI GGTGA 2 cut(s) 237, 371
AvaI CYCGRG 1 cut(s) 401
BalI TGGCCA 1 cut(s) 47
BbvI GCAGC 2 cut(s) 199, 227
BccI CCATC 1 cut(s) 14
BcnI CCSGG 2 cut(s) 402, 403
BcoDI GTCTC 1 cut(s) 354
BfmI CTRYAG 3 cut(s) 213, 411, 463
BglII AGATCT 1 cut(s) 415
BisI GCNGC 4 cut(s) 6, 213, 216, 294
BlpI GCTNAGC 1 cut(s) 443
BlsI GCNGC 4 cut(s) 7, 214, 217, 295
Bme1390I CCNGG 2 cut(s) 402, 403
BmeT110I CYCGRG 1 cut(s) 401
BmgT120I GGNCC 2 cut(s) 96, 392
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 402, 403
BmsI GCATC 2 cut(s) 267, 529
Bpu1102I GCTNAGC 1 cut(s) 443
BpuMI CCSGG 2 cut(s) 402, 403
BsaJI CCNNGG 3 cut(s) 294, 330, 401
Bsc4I CCNNNNNNNGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 320
BseDI CCNNGG 3 cut(s) 294, 330, 401
BseGI GGATG 1 cut(s) 520
BseLI CCNNNNNNNGG 1 cut(s) 401
BseMI GCAATG 1 cut(s) 320
BseXI GCAGC 2 cut(s) 199, 227
Bsh1236I CGCG 1 cut(s) 296
BshFI GGCC 3 cut(s) 47, 98, 394
BsiHKCI CYCGRG 1 cut(s) 401
BsiSI CCGG 1 cut(s) 402
BslI CCNNNNNNNGG 1 cut(s) 401
BsmAI GTCTC 1 cut(s) 354
BsnI GGCC 3 cut(s) 47, 98, 394
BsoBI CYCGRG 1 cut(s) 401
Bsp143I GATC 4 cut(s) 67, 181, 415, 568
Bsp1720I GCTNAGC 1 cut(s) 443
BspACI CCGC 4 cut(s) 294, 296, 425, 524
BspANI GGCC 3 cut(s) 47, 98, 394
BspFNI CGCG 1 cut(s) 296
BspHI TCATGA 1 cut(s) 322
BspLI GGNNCC 1 cut(s) 97
BspMAI CTGCAG 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 320
BssECI CCNNGG 3 cut(s) 294, 330, 401
BssMI GATC 4 cut(s) 67, 181, 415, 568
Bst4CI ACNGT 4 cut(s) 36, 92, 331, 515
Bst6I CTCTTC 1 cut(s) 463
BstC8I GCNNGC 2 cut(s) 196, 210
BstDEI CTNAG 2 cut(s) 289, 443
BstDSI CCRYGG 2 cut(s) 294, 330
BstF5I GGATG 1 cut(s) 520
BstFNI CGCG 1 cut(s) 296
BstHHI GCGC 1 cut(s) 488
BstKTI GATC 4 cut(s) 70, 184, 418, 571
BstMAI GTCTC 1 cut(s) 354
BstMBI GATC 4 cut(s) 67, 181, 415, 568
BstMWI GCNNNNNNNGC 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 12
BstSCI CCNGG 2 cut(s) 400, 401
BstSFI CTRYAG 3 cut(s) 213, 411, 463
BstUI CGCG 1 cut(s) 296
BstV1I GCAGC 2 cut(s) 199, 227
BstX2I RGATCY 1 cut(s) 415
BstYI RGATCY 1 cut(s) 415
BsuRI GGCC 3 cut(s) 47, 98, 394
BtgI CCRYGG 2 cut(s) 294, 330
BtsCI GGATG 1 cut(s) 520
BtsI GCAGTG 2 cut(s) 351, 437
BtsIMutI CAGTG 3 cut(s) 97, 351, 437
Cac8I GCNNGC 2 cut(s) 196, 210
CciI TCATGA 1 cut(s) 322
CfoI GCGC 1 cut(s) 488
Cfr13I GGNCC 2 cut(s) 96, 392
Cfr42I CCGCGG 1 cut(s) 297
Cfr9I CCCGGG 1 cut(s) 401
Csp6I GTAC 1 cut(s) 168
CviAII CATG 5 cut(s) 9, 155, 323, 489, 496
CviJI RGCY 9 cut(s) 5, 17, 47, 98, 127, 218, 293, 394, 493
CviKI_1 RGCY 9 cut(s) 5, 17, 47, 98, 127, 218, 293, 394, 493
CviQI GTAC 1 cut(s) 168
DdeI CTNAG 2 cut(s) 289, 443
DpnI GATC 4 cut(s) 69, 183, 417, 570
DpnII GATC 4 cut(s) 67, 181, 415, 568
EaeI YGGCCR 1 cut(s) 45
Eam1104I CTCTTC 1 cut(s) 463
EarI CTCTTC 1 cut(s) 463
Eco88I CYCGRG 1 cut(s) 401
EcoO109I RGGNCCY 1 cut(s) 96
FaeI CATG 5 cut(s) 12, 158, 326, 492, 499
FatI CATG 5 cut(s) 8, 154, 322, 488, 495
FauI CCCGC 1 cut(s) 289
Fnu4HI GCNGC 4 cut(s) 6, 213, 216, 294
FokI GGATG 1 cut(s) 507
Fsp4HI GCNGC 4 cut(s) 6, 213, 216, 294
GlaI GCGC 1 cut(s) 487
GluI GCNGC 4 cut(s) 6, 213, 216, 294
HaeIII GGCC 3 cut(s) 47, 98, 394
HapII CCGG 1 cut(s) 402
HhaI GCGC 1 cut(s) 488
Hin1II CATG 5 cut(s) 12, 158, 326, 492, 499
Hin6I GCGC 1 cut(s) 486
HinP1I GCGC 1 cut(s) 486
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 1 cut(s) 546
HpaII CCGG 1 cut(s) 402
HphI GGTGA 2 cut(s) 237, 371
Hpy188I TCNGA 2 cut(s) 84, 276
Hpy188III TCNNGA 2 cut(s) 323, 351
HpyAV CCTTC 3 cut(s) 101, 267, 554
HpyCH4III ACNGT 4 cut(s) 36, 92, 331, 515
HpyCH4V TGCA 8 cut(s) 8, 154, 176, 215, 280, 303, 520, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 14
HpyF3I CTNAG 2 cut(s) 289, 443
Hsp92II CATG 5 cut(s) 12, 158, 326, 492, 499
HspAI GCGC 1 cut(s) 486
KspI CCGCGG 1 cut(s) 297
Kzo9I GATC 4 cut(s) 67, 181, 415, 568
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 2 cut(s) 199, 227
LweI GCATC 2 cut(s) 267, 529
MalI GATC 4 cut(s) 69, 183, 417, 570
MboI GATC 4 cut(s) 67, 181, 415, 568
MboII GAAGA 2 cut(s) 410, 480
MflI RGATCY 1 cut(s) 415
MlsI TGGCCA 1 cut(s) 47
MluCI AATT 2 cut(s) 240, 364
MluNI TGGCCA 1 cut(s) 47
MnlI CCTC 3 cut(s) 52, 365, 373
Mox20I TGGCCA 1 cut(s) 47
MscI TGGCCA 1 cut(s) 47
MseI TTAA 2 cut(s) 384, 456
MslI CAYNNNNRTG 2 cut(s) 153, 203
Msp20I TGGCCA 1 cut(s) 47
MspA1I CMGCKG 1 cut(s) 296
MspI CCGG 1 cut(s) 402
MspR9I CCNGG 2 cut(s) 402, 403
MvnI CGCG 1 cut(s) 296
MwoI GCNNNNNNNGC 1 cut(s) 14
NciI CCSGG 2 cut(s) 402, 403
NdeII GATC 4 cut(s) 67, 181, 415, 568
NlaIII CATG 5 cut(s) 12, 158, 326, 492, 499
NlaIV GGNNCC 1 cut(s) 97
NspI RCATGY 1 cut(s) 12
PagI TCATGA 1 cut(s) 322
PfeI GAWTC 1 cut(s) 546
PkrI GCNGC 4 cut(s) 7, 214, 217, 295
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 2 cut(s) 96, 392
PstI CTGCAG 1 cut(s) 217
PsuI RGATCY 1 cut(s) 415
RsaI GTAC 1 cut(s) 169
RsaNI GTAC 1 cut(s) 168
RseI CAYNNNNRTG 2 cut(s) 153, 203
SacII CCGCGG 1 cut(s) 297
SaqAI TTAA 2 cut(s) 384, 456
SatI GCNGC 4 cut(s) 6, 213, 216, 294
Sau3AI GATC 4 cut(s) 67, 181, 415, 568
Sau96I GGNCC 2 cut(s) 96, 392
ScrFI CCNGG 2 cut(s) 402, 403
SetI ASST 8 cut(s) 63, 129, 181, 220, 250, 376, 384, 565
SfaNI GCATC 2 cut(s) 267, 529
SfcI CTRYAG 3 cut(s) 213, 411, 463
Sfr303I CCGCGG 1 cut(s) 297
SgrBI CCGCGG 1 cut(s) 297
SmaI CCCGGG 1 cut(s) 403
SmiMI CAYNNNNRTG 2 cut(s) 153, 203
Sse9I AATT 2 cut(s) 240, 364
SsiI CCGC 4 cut(s) 294, 296, 425, 524
StyD4I CCNGG 2 cut(s) 400, 401
TaaI ACNGT 4 cut(s) 36, 92, 331, 515
TaqI TCGA 2 cut(s) 76, 352
TasI AATT 2 cut(s) 240, 364
TatI WGTACW 1 cut(s) 167
TauI GCSGC 1 cut(s) 296
TfiI GAWTC 1 cut(s) 546
Tru1I TTAA 2 cut(s) 384, 456
Tru9I TTAA 2 cut(s) 384, 456
TscAI CASTG 3 cut(s) 97, 351, 444
TseI GCWGC 3 cut(s) 5, 212, 215
TspDTI ATGAA 2 cut(s) 223, 573
TspMI CCCGGG 1 cut(s) 401
TspRI CASTG 3 cut(s) 97, 351, 444
XapI RAATTY 1 cut(s) 364
XceI RCATGY 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.