Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0000963

Physical Location & Seq

Genomic Coordinates Forward (+)

33916426 .. 33919753

Gene Structure 3328 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1251500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1146 bp

ATGCAGCGTAAGCAAAAGCCAACTATGGAAGAAAAGGCATCTGCCGGGAAGAGTATGTACGGTGGTTCTCTTCTTGTTCCTAGTGTTCAGGAATTAGTAAAGAAGCCAATAGTCAAAATCCCACCCAGATACATACAGCCTCATCATCAAGATGGTAATTACCAAGAGCATGACATGAACATCAATTCTGAGCATCTTGCTGACCAGATACCATCCATTGACATGCATAAATTGCTTTCCAAAGAATCAACAAATTCTGAAGCTGAACTAGCCAGGCTCCATTTTGCTTGCAAAGAGTGGGGTTTCTTCCAGTTGGTAAACCATGGTGTGAGCTCTTCCTTGTTGGAGAAAATGAAGACAGAAACTCAAGAATTCTTCAACCTACCCATGGAAGAGAAGAAAAAGTTTTGGCAACAGCCAGGAGACGTGGAAGGTTTTGGACAAGCCTTTATAATTTCTGAAAACCAAAAACTCGATTGGGTTGACATGTTCTTCTTGAATACCCTTCCTGTCCAAATGAGGAGGCCTCACCTATTTCCAAATCTCCCTTCTCCTTTCAGAGAGACCTTAGAAACTTACTCATTGGAACTGAAAAACCTAGCCATGACTATCCTATCACACATTGAAAAAGCTTTGCAGATGGAAGCCAAGGAAGTGACCAAGTTATTTGAAGATGGACTGCAAGCGACGAGGATGAACTATTACCCTCCATGTCCTCAGCCGGAGGCAGTCATCGGTCTGACCCCTCACTCTGATCCTGTAGGCCTCACCGTCCTCCTACAAGTCAATGAAATGGATGGTCTCCAAGTAAAGAGAGATGGGATTTGGGTTCCTATTAAGCCACTTCCAGATGCATTTGTTGTGAATATTGGAGACATTCTAGAGATTCTAACAAATGGGACGTATCGTAGCATTGAGCATCGTGCAACTGTGAACTCTGTAAAAGAAAGGCTCTCAATCGCCGCATTTCACAGCCCTAGATTTGGTGGTGAAATTGGCCCGGCCTCTAGCTTGATCACAGGACAAACACCAGCAACATATATAAGGGTGGGAGTTGAAGAATATTTCAAAGCCTTATTTGAACGTAAGCTTATTGGAAAGTCTTTTCTTGATGAACTGAAGATAAAGCATGATGGTGAGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

382

Amino Acids

43.31

Weight (kDa)

6.04

Isoelectric Point (pI)

42.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	70 - 180	1.5e-30	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	228 - 326	2.2e-32	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17010 AT1G17020 AT4G25300 AT4G25300 AT4G25310 AT4G25310
fragaria_vesca	FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06512 FvH4_3g06513 FvH4_3g06513 FvH4_3g06513 FvH4_3g17470
malus_domestica	MD03G1251500.v1.1 MD11G1271400.v1.1 MD11G1272000.v1.1 MD11G1272100.v1.1 MD11G1272300.v1.1 MD11G1272400.v1.1 MD11G1272500.v1.1
prunus_persica	Prupe.4G154800_v2.0.a1 Prupe.4G154800_v2.0.a1 Prupe.4G154900_v2.0.a1 Prupe.4G155000_v2.0.a1 Prupe.4G155100_v2.0.a1
pyrus_communis	pycom11g23990 pycom11g24060 pycom11g24070
rosa_chinensis	RchiOBHm_Chr5g0012661 RchiOBHm_Chr5g0028991 RchiOBHm_Chr5g0029011
rosa_laevigata	RLG00000031946 RLG00000033134
rosa_multiflora	Rmu_sc0003577.1_g000001 Rmu_sc0009042.1_g000002 Rmu_sc0011332.1_g000001 Rmu_sc0011896.1_g000003
rosa_roxburghii	Rroxscaffold_1G00050860 Rroxscaffold_1G00050890 Rroxscaffold_1G00063940
rosa_rugosa	Rorug05G0003500 Rorug05G0112200
rosa_samantha	Rh5AG097300 Rh5AG204300 Rh5AG204400 Rh5BG093700 Rh5BG202600 Rh5BG202700 Rh5CG223500 Rh5CG223600 Rh5DG092800 Rh5DG206300 Rh5DG206400
rosa_wichuraiana	Rw5G008530 Rw5G018520 Rw5G018530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	452
AciI	CCGC	1 cut(s)	963
AclWI	GGATC	1 cut(s)	749
AcsI	RAATTY	2 cut(s)	253, 371
AcuI	CTGAAG	2 cut(s)	279, 1139
AfaI	GTAC	1 cut(s)	59
AfiI	CCNNNNNNNGG	2 cut(s)	388, 983
AflIII	ACRYGT	1 cut(s)	486
AgsI	TTSAA	7 cut(s)	379, 499, 626, 671, 1058, 1069, 1082
AjiI	CACGTC	1 cut(s)	427
AjnI	CCWGG	2 cut(s)	272, 418
AjuI	GAANNNNNNNTTGG	2 cut(s)	532, 564
AluBI	AGCT	5 cut(s)	263, 333, 632, 1011, 1090
AluI	AGCT	5 cut(s)	263, 333, 632, 1011, 1090
Alw21I	GWGCWC	1 cut(s)	335
Alw26I	GTCTC	4 cut(s)	417, 557, 806, 867
AlwI	GGATC	1 cut(s)	749
AoxI	GGCC	4 cut(s)	524, 763, 997, 1002
ApeKI	GCWGC	1 cut(s)	4
ApoI	RAATTY	2 cut(s)	253, 371
AspS9I	GGNCC	1 cut(s)	998
AsuC2I	CCSGG	2 cut(s)	46, 1001
AsuHPI	GGTGA	3 cut(s)	521, 760, 1001
BanII	GRGCYC	1 cut(s)	335
BarI	GAAGNNNNNNTAC	2 cut(s)	41, 73
BbsI	GAAGAC	1 cut(s)	362
Bbv12I	GWGCWC	1 cut(s)	335
BbvCI	CCTCAGC	1 cut(s)	717
BbvI	GCAGC	1 cut(s)	16
BccI	CCATC	7 cut(s)	146, 220, 634, 668, 791, 812, 1127
BciT130I	CCWGG	2 cut(s)	274, 420
BclI	TGATCA	1 cut(s)	1014
BcnI	CCSGG	2 cut(s)	46, 1001
BcoDI	GTCTC	4 cut(s)	417, 557, 806, 867
BfaI	CTAG	6 cut(s)	81, 269, 599, 881, 978, 1008
BfmI	CTRYAG	1 cut(s)	759
BisI	GCNGC	2 cut(s)	5, 963
BlsI	GCNGC	2 cut(s)	6, 964
Bme1390I	CCNGG	4 cut(s)	46, 274, 420, 1001
BmgBI	CACGTC	1 cut(s)	427
BmgT120I	GGNCC	1 cut(s)	998
BmiI	GGNNCC	2 cut(s)	278, 831
BmrFI	CCNGG	4 cut(s)	46, 274, 420, 1001
BmsI	GCATC	4 cut(s)	47, 202, 841, 928
BpiI	GAAGAC	1 cut(s)	362
BplI	GAGNNNNNCTC	2 cut(s)	511, 543
Bpu10I	CCTNAGC	1 cut(s)	717
BpuEI	CTTGAG	1 cut(s)	351
BpuMI	CCSGG	2 cut(s)	46, 1001
BsaI	GGTCTC	2 cut(s)	557, 806
BsaJI	CCNNGG	3 cut(s)	322, 387, 648
Bsc4I	CCNNNNNNNGG	2 cut(s)	388, 983
Bse1I	ACTGG	1 cut(s)	310
BseBI	CCWGG	2 cut(s)	274, 420
BseDI	CCNNGG	3 cut(s)	322, 387, 648
BseGI	GGATG	3 cut(s)	212, 699, 802
BseLI	CCNNNNNNNGG	2 cut(s)	388, 983
BseMII	CTCAG	2 cut(s)	180, 731
BseNI	ACTGG	1 cut(s)	310
BseRI	GAGGAG	1 cut(s)	535
BseXI	GCAGC	1 cut(s)	16
BshFI	GGCC	4 cut(s)	526, 765, 999, 1004
BsiHKAI	GWGCWC	1 cut(s)	335
BsiSI	CCGG	3 cut(s)	45, 722, 1001
BslFI	GGGAC	1 cut(s)	913
BslI	CCNNNNNNNGG	2 cut(s)	388, 983
BsmAI	GTCTC	4 cut(s)	417, 557, 806, 867
BsmBI	CGTCTC	1 cut(s)	417
BsmFI	GGGAC	1 cut(s)	913
BsnI	GGCC	4 cut(s)	526, 765, 999, 1004
Bso31I	GGTCTC	2 cut(s)	557, 806
Bsp1286I	GDGCHC	1 cut(s)	335
Bsp143I	GATC	2 cut(s)	754, 1014
Bsp19I	CCATGG	2 cut(s)	322, 387
BspACI	CCGC	1 cut(s)	963
BspANI	GGCC	4 cut(s)	526, 765, 999, 1004
BspCNI	CTCAG	2 cut(s)	181, 730
BspLI	GGNNCC	2 cut(s)	278, 831
BspPI	GGATC	1 cut(s)	749
BspQI	GCTCTTC	1 cut(s)	340
BspTNI	GGTCTC	2 cut(s)	557, 806
BsrI	ACTGG	1 cut(s)	310
BssECI	CCNNGG	3 cut(s)	322, 387, 648
BssMI	GATC	2 cut(s)	754, 1014
BssT1I	CCWWGG	3 cut(s)	322, 387, 648
Bst2UI	CCWGG	2 cut(s)	274, 420
Bst4CI	ACNGT	3 cut(s)	62, 772, 931
Bst6I	CTCTTC	4 cut(s)	44, 75, 340, 387
BstAPI	GCANNNNNTGC	1 cut(s)	232
BstC8I	GCNNGC	2 cut(s)	289, 684
BstDEI	CTNAG	3 cut(s)	189, 568, 717
BstDSI	CCRYGG	2 cut(s)	322, 387
BstF5I	GGATG	3 cut(s)	212, 699, 802
BstKTI	GATC	2 cut(s)	757, 1017
BstMAI	GTCTC	4 cut(s)	417, 557, 806, 867
BstMBI	GATC	2 cut(s)	754, 1014
BstMWI	GCNNNNNNNGC	3 cut(s)	10, 232, 269
BstNI	CCWGG	2 cut(s)	274, 420
BstNSI	RCATGY	2 cut(s)	226, 490
BstSCI	CCNGG	4 cut(s)	44, 272, 418, 999
BstSFI	CTRYAG	1 cut(s)	759
BstV1I	GCAGC	1 cut(s)	16
BstV2I	GAAGAC	1 cut(s)	362
BsuRI	GGCC	4 cut(s)	526, 765, 999, 1004
BtgI	CCRYGG	2 cut(s)	322, 387
BtrI	CACGTC	1 cut(s)	427
BtsCI	GGATG	3 cut(s)	212, 699, 802
Cac8I	GCNNGC	2 cut(s)	289, 684
Cfr13I	GGNCC	1 cut(s)	998
Csp6I	GTAC	1 cut(s)	58
CviAII	CATG	9 cut(s)	170, 175, 223, 323, 388, 487, 604, 711, 1130
CviQI	GTAC	1 cut(s)	58
DdeI	CTNAG	3 cut(s)	189, 568, 717
DpnI	GATC	2 cut(s)	756, 1016
DpnII	GATC	2 cut(s)	754, 1014
Eam1104I	CTCTTC	4 cut(s)	44, 75, 340, 387
EarI	CTCTTC	4 cut(s)	44, 75, 340, 387
Ecl136II	GAGCTC	1 cut(s)	333
Eco130I	CCWWGG	3 cut(s)	322, 387, 648
Eco147I	AGGCCT	2 cut(s)	526, 765
Eco24I	GRGCYC	1 cut(s)	335
Eco31I	GGTCTC	2 cut(s)	557, 806
Eco53kI	GAGCTC	1 cut(s)	333
Eco57I	CTGAAG	2 cut(s)	279, 1139
EcoICRI	GAGCTC	1 cut(s)	333
EcoRI	GAATTC	1 cut(s)	371
EcoRII	CCWGG	2 cut(s)	272, 418
EcoT14I	CCWWGG	3 cut(s)	322, 387, 648
EcoT22I	ATGCAT	2 cut(s)	228, 856
EcoT38I	GRGCYC	1 cut(s)	335
ErhI	CCWWGG	3 cut(s)	322, 387, 648
Esp3I	CGTCTC	1 cut(s)	417
FaeI	CATG	9 cut(s)	173, 178, 226, 326, 391, 490, 607, 714, 1133
FaqI	GGGAC	1 cut(s)	913
FatI	CATG	9 cut(s)	169, 174, 222, 322, 387, 486, 603, 710, 1129
FbaI	TGATCA	1 cut(s)	1014
Fnu4HI	GCNGC	2 cut(s)	5, 963
FokI	GGATG	3 cut(s)	199, 706, 809
FriOI	GRGCYC	1 cut(s)	335
Fsp4HI	GCNGC	2 cut(s)	5, 963
FspBI	CTAG	6 cut(s)	81, 269, 599, 881, 978, 1008
GluI	GCNGC	2 cut(s)	5, 963
HaeIII	GGCC	4 cut(s)	526, 765, 999, 1004
HapII	CCGG	3 cut(s)	45, 722, 1001
Hin1II	CATG	9 cut(s)	173, 178, 226, 326, 391, 490, 607, 714, 1133
HincII	GTYRAC	1 cut(s)	484
HindII	GTYRAC	1 cut(s)	484
HindIII	AAGCTT	2 cut(s)	630, 1088
HinfI	GANTC	2 cut(s)	245, 886
HpaII	CCGG	3 cut(s)	45, 722, 1001
HphI	GGTGA	3 cut(s)	521, 760, 1001
Hpy166II	GTNNAC	3 cut(s)	319, 484, 934
Hpy188I	TCNGA	6 cut(s)	190, 259, 460, 560, 741, 754
Hpy188III	TCNNGA	7 cut(s)	89, 149, 368, 496, 848, 881, 1109
Hpy8I	GTNNAC	3 cut(s)	319, 484, 934
Hpy99I	CGWCG	1 cut(s)	691
HpyAV	CCTTC	3 cut(s)	425, 515, 558
HpyCH4III	ACNGT	3 cut(s)	62, 772, 931
HpyCH4IV	ACGT	3 cut(s)	426, 902, 1084
HpyCH4V	TGCA	7 cut(s)	4, 226, 291, 637, 682, 854, 926
HpyF10VI	GCNNNNNNNGC	3 cut(s)	10, 232, 269
HpyF3I	CTNAG	3 cut(s)	189, 568, 717
HpySE526I	ACGT	3 cut(s)	426, 902, 1084
Hsp92II	CATG	9 cut(s)	173, 178, 226, 326, 391, 490, 607, 714, 1133
Ksp22I	TGATCA	1 cut(s)	1014
Kzo9I	GATC	2 cut(s)	754, 1014
LguI	GCTCTTC	1 cut(s)	340
LmnI	GCTCC	1 cut(s)	282
Lsp1109I	GCAGC	1 cut(s)	16
LweI	GCATC	4 cut(s)	47, 202, 841, 928
MaeI	CTAG	6 cut(s)	81, 269, 599, 881, 978, 1008
MaeII	ACGT	3 cut(s)	426, 902, 1084
MaeIII	GTNAC	1 cut(s)	655
MalI	GATC	2 cut(s)	756, 1016
MboI	GATC	2 cut(s)	754, 1014
MhlI	GDGCHC	1 cut(s)	335
MluCI	AATT	8 cut(s)	92, 157, 184, 230, 253, 371, 453, 993
MmeI	TCCRAC	1 cut(s)	324
Mph1103I	ATGCAT	2 cut(s)	228, 856
MseI	TTAA	1 cut(s)	837
MslI	CAYNNNNRTG	3 cut(s)	150, 221, 1134
MspI	CCGG	3 cut(s)	45, 722, 1001
MspR9I	CCNGG	4 cut(s)	46, 274, 420, 1001
MvaI	CCWGG	2 cut(s)	274, 420
MwoI	GCNNNNNNNGC	3 cut(s)	10, 232, 269
NciI	CCSGG	2 cut(s)	46, 1001
NcoI	CCATGG	2 cut(s)	322, 387
NdeII	GATC	2 cut(s)	754, 1014
NlaIII	CATG	9 cut(s)	173, 178, 226, 326, 391, 490, 607, 714, 1133
NlaIV	GGNNCC	2 cut(s)	278, 831
NmuCI	GTSAC	1 cut(s)	655
NsiI	ATGCAT	2 cut(s)	228, 856
NspI	RCATGY	2 cut(s)	226, 490
PceI	AGGCCT	2 cut(s)	526, 765
PciI	ACATGT	1 cut(s)	486
PciSI	GCTCTTC	1 cut(s)	340
PfeI	GAWTC	2 cut(s)	245, 886
PkrI	GCNGC	2 cut(s)	6, 964
PscI	ACATGT	1 cut(s)	486
PsiI	TTATAA	1 cut(s)	452
Psp124BI	GAGCTC	1 cut(s)	335
Psp6I	CCWGG	2 cut(s)	272, 418
PspGI	CCWGG	2 cut(s)	272, 418
PspN4I	GGNNCC	2 cut(s)	278, 831
PspPI	GGNCC	1 cut(s)	998
RsaI	GTAC	1 cut(s)	59
RsaNI	GTAC	1 cut(s)	58
RseI	CAYNNNNRTG	3 cut(s)	150, 221, 1134
SacI	GAGCTC	1 cut(s)	335
SapI	GCTCTTC	1 cut(s)	340
SaqAI	TTAA	1 cut(s)	837
SatI	GCNGC	2 cut(s)	5, 963
Sau3AI	GATC	2 cut(s)	754, 1014
Sau96I	GGNCC	1 cut(s)	998
ScrFI	CCNGG	4 cut(s)	46, 274, 420, 1001
SduI	GDGCHC	1 cut(s)	335
SfaNI	GCATC	4 cut(s)	47, 202, 841, 928
SfcI	CTRYAG	1 cut(s)	759
SmiMI	CAYNNNNRTG	3 cut(s)	150, 221, 1134
SmlI	CTYRAG	1 cut(s)	366
SmoI	CTYRAG	1 cut(s)	366
Sse9I	AATT	8 cut(s)	92, 157, 184, 230, 253, 371, 453, 993
SseBI	AGGCCT	2 cut(s)	526, 765
SsiI	CCGC	1 cut(s)	963
SspI	AATATT	2 cut(s)	868, 1064
SspMI	CTAG	6 cut(s)	81, 269, 599, 881, 978, 1008
SstI	GAGCTC	1 cut(s)	335
StuI	AGGCCT	2 cut(s)	526, 765
StyD4I	CCNGG	4 cut(s)	44, 272, 418, 999
StyI	CCWWGG	3 cut(s)	322, 387, 648
TaaI	ACNGT	3 cut(s)	62, 772, 931
TaiI	ACGT	3 cut(s)	429, 905, 1087
TaqI	TCGA	1 cut(s)	474
TaqII	GACCGA	1 cut(s)	725
TasI	AATT	8 cut(s)	92, 157, 184, 230, 253, 371, 453, 993
TauI	GCSGC	1 cut(s)	965
TfiI	GAWTC	2 cut(s)	245, 886
Tru1I	TTAA	1 cut(s)	837
Tru9I	TTAA	1 cut(s)	837
TseFI	GTSAC	1 cut(s)	655
TseI	GCWGC	1 cut(s)	4
Tsp45I	GTSAC	1 cut(s)	655
TspDTI	ATGAA	5 cut(s)	191, 368, 710, 804, 1128
XapI	RAATTY	2 cut(s)	253, 371
XbaI	TCTAGA	1 cut(s)	880
XceI	RCATGY	2 cut(s)	226, 490
XspI	CTAG	6 cut(s)	81, 269, 599, 881, 978, 1008
Zsp2I	ATGCAT	2 cut(s)	228, 856

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.