Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0000963

Physical Location & Seq

Genomic Coordinates Reverse (-)

8446341 .. 8449209

Gene Structure 2869 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG093700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1038 bp

ATGGAAGAATTAGCAAAGCTAGGGAGCTCTCTTCCGGTGCCTTGTGTTCAGGAGCTTGCGAAAGAGCCATTAACTACAGTCCCACACCGATATGTTCGCCCTGATCAAGATCCTCCCTTCATTTCTGGTATGGCCACTTCTTCACCTCAAGTACCAGTGATCGACATGAACAAGTTACTTTCAGAAGAAACCATGCCAGCAGAGTTGCAGGAGTTCCACCTTGCTTGCAGAGAATGGGGTTTCTTCCAGATATGCAAAGAATCCGTGGTTTTAACACATGGAGTTGGTGAAACTTTGTTGGAGAGTGTGAAGTCAGGGATTCAAGAATTCTTCAGTCTGCCAATGGAAGAGAAGAAACAGTTTTGGCAGCAGCCAGAAATTATGGAGGGTTTTGGACAAGCATTTGTTGTGTCTGAGAACCAGAAACTTGATTGGTCAGACATATTCTACTTGATCACACTCCCAAAACATTTGAGGAAGCCACATTTATTCCCCAATATTCCTCAACCACTAAGAGATAACTTAGAAGTGTACTCTGCGGAACTAGAAGATCTTGCCAAGAAGATTCTTGACCTCATGGCAAAAGCACTGGGAGTGGATGCTAACTATATAAAAGAGTTGTTTGACCAAGGGACTCAGATGTTTAGGATGAACTATTATCCACCATGTAAGCAACCAGAGCTTGTCATTGGCCTTAACCCTCACTCAGATGCTGTTGGATTGACCATCCTCCTCCAAGTGAATGAAATGGAAGGCCTTCAGATTGTGACGAATGGAATATACCGTAGCATTGAGCATCGGGCAACAGTGAACCAGGAGAAGGAAAGGCTGTCAATAGCCACATTTTACAGTCCAAAATTGGAGGGAGATATGGGTCCTGCTCCGAACCTCATTACTCCAGAGATACCAGCATTGTTCAAGAGAATAGGTGTTGCAGACTACTTCAGGGGATTTTATGAACGCAAACTCGATGGGAAATCATACATAGATGTCATGAGGATTCAACATGGTGAGAATGGCAAAGGCACCCAAGACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

345

Amino Acids

39.2

Weight (kDa)

5.13

Isoelectric Point (pI)

44.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	51 - 165	3e-24	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	212 - 255	1.7e-10	2OG-Fe(II) oxygenase superfamily
2OG-FeII_Oxy	PF03171	248 - 285	1.1e-06	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17010 AT1G17020 AT4G25300 AT4G25300 AT4G25310 AT4G25310
fragaria_vesca	FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06512 FvH4_3g06513 FvH4_3g06513 FvH4_3g06513 FvH4_3g17470
malus_domestica	MD03G1251500.v1.1 MD11G1271400.v1.1 MD11G1272000.v1.1 MD11G1272100.v1.1 MD11G1272300.v1.1 MD11G1272400.v1.1 MD11G1272500.v1.1
prunus_persica	Prupe.4G154800_v2.0.a1 Prupe.4G154800_v2.0.a1 Prupe.4G154900_v2.0.a1 Prupe.4G155000_v2.0.a1 Prupe.4G155100_v2.0.a1
pyrus_communis	pycom11g23990 pycom11g24060 pycom11g24070
rosa_chinensis	RchiOBHm_Chr5g0012661 RchiOBHm_Chr5g0028991 RchiOBHm_Chr5g0029011
rosa_laevigata	RLG00000031946 RLG00000033134
rosa_multiflora	Rmu_sc0003577.1_g000001 Rmu_sc0009042.1_g000002 Rmu_sc0011332.1_g000001 Rmu_sc0011896.1_g000003
rosa_roxburghii	Rroxscaffold_1G00050860 Rroxscaffold_1G00050890 Rroxscaffold_1G00063940
rosa_rugosa	Rorug05G0003500 Rorug05G0112200
rosa_samantha	Rh5AG097300 Rh5AG204300 Rh5AG204400 Rh5BG093700 Rh5BG202600 Rh5BG202700 Rh5CG223500 Rh5CG223600 Rh5DG092800 Rh5DG206300 Rh5DG206400
rosa_wichuraiana	Rw5G008530 Rw5G018520 Rw5G018530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	37, 1025
AciI	CCGC	1 cut(s)	539
AclWI	GGATC	1 cut(s)	104
AcoI	YGGCCR	1 cut(s)	132
AcsI	RAATTY	1 cut(s)	326
AcuI	CTGAAG	3 cut(s)	316, 743, 928
AfaI	GTAC	2 cut(s)	153, 533
AfiI	CCNNNNNNNGG	1 cut(s)	820
AgsI	TTSAA	3 cut(s)	323, 919, 1004
AjnI	CCWGG	1 cut(s)	813
AluBI	AGCT	4 cut(s)	19, 27, 55, 682
AluI	AGCT	4 cut(s)	19, 27, 55, 682
Alw21I	GWGCWC	1 cut(s)	29
AlwI	GGATC	1 cut(s)	104
AlwNI	CAGNNNCTG	1 cut(s)	713
AoxI	GGCC	3 cut(s)	132, 691, 754
ApeKI	GCWGC	2 cut(s)	367, 370
ApoI	RAATTY	1 cut(s)	326
Asp700I	GAANNNNTTC	1 cut(s)	756
AspS9I	GGNCC	1 cut(s)	875
AsuHPI	GGTGA	3 cut(s)	135, 299, 1022
AvaII	GGWCC	1 cut(s)	875
BalI	TGGCCA	1 cut(s)	134
BanI	GGYRCC	2 cut(s)	37, 1025
BanII	GRGCYC	1 cut(s)	29
BarI	GAAGNNNNNNTAC	2 cut(s)	121, 153
Bbv12I	GWGCWC	1 cut(s)	29
BbvI	GCAGC	2 cut(s)	379, 382
BccI	CCATC	2 cut(s)	734, 965
BciT130I	CCWGG	1 cut(s)	815
BclI	TGATCA	2 cut(s)	103, 453
BfaI	CTAG	3 cut(s)	20, 545, 1036
BfmI	CTRYAG	1 cut(s)	75
BglII	AGATCT	1 cut(s)	550
BisI	GCNGC	2 cut(s)	368, 371
BlsI	GCNGC	2 cut(s)	369, 372
Bme1390I	CCNGG	1 cut(s)	815
Bme18I	GGWCC	1 cut(s)	875
BmgT120I	GGNCC	1 cut(s)	875
BmiI	GGNNCC	3 cut(s)	39, 876, 1027
BmrFI	CCNGG	1 cut(s)	815
BmrI	ACTGGG	1 cut(s)	599
BmsI	GCATC	3 cut(s)	589, 700, 805
BmuI	ACTGGG	1 cut(s)	599
BpmI	CTGGAG	1 cut(s)	882
BpuEI	CTTGAG	1 cut(s)	132
BsaBI	GATNNNNATC	1 cut(s)	108
BsaJI	CCNNGG	2 cut(s)	264, 628
BsaWI	WCCGGW	1 cut(s)	34
Bsc4I	CCNNNNNNNGG	1 cut(s)	820
Bse1I	ACTGG	2 cut(s)	155, 594
Bse8I	GATNNNNATC	1 cut(s)	108
BseBI	CCWGG	1 cut(s)	815
BseDI	CCNNGG	2 cut(s)	264, 628
BseGI	GGATG	3 cut(s)	604, 654, 726
BseJI	GATNNNNATC	1 cut(s)	108
BseLI	CCNNNNNNNGG	1 cut(s)	820
BseMII	CTCAG	3 cut(s)	405, 650, 720
BseNI	ACTGG	2 cut(s)	155, 594
BseRI	GAGGAG	1 cut(s)	722
BseXI	GCAGC	2 cut(s)	379, 382
BshFI	GGCC	3 cut(s)	134, 693, 756
BshNI	GGYRCC	2 cut(s)	37, 1025
BsiHKAI	GWGCWC	1 cut(s)	29
BsiSI	CCGG	1 cut(s)	35
BslFI	GGGAC	2 cut(s)	65, 646
BslI	CCNNNNNNNGG	1 cut(s)	820
BsmFI	GGGAC	2 cut(s)	65, 646
BsnI	GGCC	3 cut(s)	134, 693, 756
Bsp1286I	GDGCHC	1 cut(s)	29
Bsp143I	GATC	5 cut(s)	103, 109, 159, 453, 550
BspACI	CCGC	1 cut(s)	539
BspANI	GGCC	3 cut(s)	134, 693, 756
BspCNI	CTCAG	3 cut(s)	406, 649, 719
BspHI	TCATGA	1 cut(s)	993
BspLI	GGNNCC	3 cut(s)	39, 876, 1027
BspPI	GGATC	1 cut(s)	104
BspT107I	GGYRCC	2 cut(s)	37, 1025
BsrI	ACTGG	2 cut(s)	155, 594
BssECI	CCNNGG	2 cut(s)	264, 628
BssMI	GATC	5 cut(s)	103, 109, 159, 453, 550
BssT1I	CCWWGG	1 cut(s)	628
Bst2UI	CCWGG	1 cut(s)	815
Bst4CI	ACNGT	5 cut(s)	79, 360, 785, 808, 851
Bst6I	CTCTTC	2 cut(s)	36, 342
BstC8I	GCNNGC	3 cut(s)	57, 198, 226
BstDEI	CTNAG	5 cut(s)	414, 512, 523, 636, 706
BstDSI	CCRYGG	1 cut(s)	264
BstF5I	GGATG	3 cut(s)	604, 654, 726
BstKTI	GATC	5 cut(s)	106, 112, 162, 456, 553
BstMBI	GATC	5 cut(s)	103, 109, 159, 453, 550
BstMWI	GCNNNNNNNGC	1 cut(s)	679
BstNI	CCWGG	1 cut(s)	815
BstSCI	CCNGG	1 cut(s)	813
BstSFI	CTRYAG	1 cut(s)	75
BstV1I	GCAGC	2 cut(s)	379, 382
BstX2I	RGATCY	2 cut(s)	109, 550
BstYI	RGATCY	2 cut(s)	109, 550
BsuRI	GGCC	3 cut(s)	134, 693, 756
BtgI	CCRYGG	1 cut(s)	264
BtsCI	GGATG	3 cut(s)	604, 654, 726
BtsIMutI	CAGTG	3 cut(s)	162, 587, 813
Cac8I	GCNNGC	3 cut(s)	57, 198, 226
CaiI	CAGNNNCTG	1 cut(s)	713
CciI	TCATGA	1 cut(s)	993
Cfr13I	GGNCC	1 cut(s)	875
Csp6I	GTAC	2 cut(s)	152, 532
CspCI	CAANNNNNGTGG	2 cut(s)	206, 241
CviAII	CATG	7 cut(s)	166, 193, 278, 577, 666, 994, 1007
CviQI	GTAC	2 cut(s)	152, 532
DdeI	CTNAG	5 cut(s)	414, 512, 523, 636, 706
DpnI	GATC	5 cut(s)	105, 111, 161, 455, 552
DpnII	GATC	5 cut(s)	103, 109, 159, 453, 550
EaeI	YGGCCR	1 cut(s)	132
Eam1104I	CTCTTC	2 cut(s)	36, 342
EarI	CTCTTC	2 cut(s)	36, 342
Ecl136II	GAGCTC	1 cut(s)	27
Eco130I	CCWWGG	1 cut(s)	628
Eco147I	AGGCCT	1 cut(s)	756
Eco24I	GRGCYC	1 cut(s)	29
Eco47I	GGWCC	1 cut(s)	875
Eco53kI	GAGCTC	1 cut(s)	27
Eco57I	CTGAAG	3 cut(s)	316, 743, 928
EcoICRI	GAGCTC	1 cut(s)	27
EcoO109I	RGGNCCY	1 cut(s)	875
EcoRI	GAATTC	1 cut(s)	326
EcoRII	CCWGG	1 cut(s)	813
EcoT14I	CCWWGG	1 cut(s)	628
EcoT38I	GRGCYC	1 cut(s)	29
ErhI	CCWWGG	1 cut(s)	628
FaeI	CATG	7 cut(s)	169, 196, 281, 580, 669, 997, 1010
FaqI	GGGAC	2 cut(s)	65, 646
FatI	CATG	7 cut(s)	165, 192, 277, 576, 665, 993, 1006
FbaI	TGATCA	2 cut(s)	103, 453
Fnu4HI	GCNGC	2 cut(s)	368, 371
FokI	GGATG	3 cut(s)	611, 661, 713
FriOI	GRGCYC	1 cut(s)	29
Fsp4HI	GCNGC	2 cut(s)	368, 371
FspBI	CTAG	3 cut(s)	20, 545, 1036
GluI	GCNGC	2 cut(s)	368, 371
GsuI	CTGGAG	1 cut(s)	882
HaeIII	GGCC	3 cut(s)	134, 693, 756
HapII	CCGG	1 cut(s)	35
Hin1II	CATG	7 cut(s)	169, 196, 281, 580, 669, 997, 1010
HinfI	GANTC	5 cut(s)	260, 319, 565, 634, 1000
HpaII	CCGG	1 cut(s)	35
HphI	GGTGA	3 cut(s)	135, 299, 1022
Hpy166II	GTNNAC	2 cut(s)	532, 811
Hpy188I	TCNGA	7 cut(s)	184, 415, 439, 639, 709, 762, 885
Hpy188III	TCNNGA	8 cut(s)	50, 107, 247, 323, 569, 899, 919, 994
Hpy8I	GTNNAC	2 cut(s)	532, 811
HpyAV	CCTTC	4 cut(s)	127, 746, 767, 814
HpyCH4III	ACNGT	5 cut(s)	79, 360, 785, 808, 851
HpyCH4V	TGCA	4 cut(s)	208, 228, 255, 935
HpyF10VI	GCNNNNNNNGC	1 cut(s)	679
HpyF3I	CTNAG	5 cut(s)	414, 512, 523, 636, 706
Hsp92II	CATG	7 cut(s)	169, 196, 281, 580, 669, 997, 1010
Ksp22I	TGATCA	2 cut(s)	103, 453
Kzo9I	GATC	5 cut(s)	103, 109, 159, 453, 550
LmnI	GCTCC	3 cut(s)	24, 52, 886
Lsp1109I	GCAGC	2 cut(s)	379, 382
LweI	GCATC	3 cut(s)	589, 700, 805
MaeI	CTAG	3 cut(s)	20, 545, 1036
MaeIII	GTNAC	2 cut(s)	174, 766
MalI	GATC	5 cut(s)	105, 111, 161, 455, 552
MboI	GATC	5 cut(s)	103, 109, 159, 453, 550
MflI	RGATCY	2 cut(s)	109, 550
MhlI	GDGCHC	1 cut(s)	29
MlsI	TGGCCA	1 cut(s)	134
MluCI	AATT	4 cut(s)	8, 326, 378, 857
MluNI	TGGCCA	1 cut(s)	134
MlyI	GAGTC	1 cut(s)	628
MmeI	TCCRAC	2 cut(s)	279, 697
Mox20I	TGGCCA	1 cut(s)	134
MroXI	GAANNNNTTC	1 cut(s)	756
MscI	TGGCCA	1 cut(s)	134
MseI	TTAA	3 cut(s)	71, 272, 696
MslI	CAYNNNNRTG	2 cut(s)	90, 708
Msp20I	TGGCCA	1 cut(s)	134
MspI	CCGG	1 cut(s)	35
MspR9I	CCNGG	1 cut(s)	815
MvaI	CCWGG	1 cut(s)	815
MwoI	GCNNNNNNNGC	1 cut(s)	679
NdeII	GATC	5 cut(s)	103, 109, 159, 453, 550
NlaIII	CATG	7 cut(s)	169, 196, 281, 580, 669, 997, 1010
NlaIV	GGNNCC	3 cut(s)	39, 876, 1027
NmuCI	GTSAC	1 cut(s)	766
PagI	TCATGA	1 cut(s)	993
PceI	AGGCCT	1 cut(s)	756
PdmI	GAANNNNTTC	1 cut(s)	756
PfeI	GAWTC	4 cut(s)	260, 319, 565, 1000
PkrI	GCNGC	2 cut(s)	369, 372
PleI	GAGTC	1 cut(s)	628
PpsI	GAGTC	1 cut(s)	628
PpuMI	RGGWCCY	1 cut(s)	875
Psp124BI	GAGCTC	1 cut(s)	29
Psp5II	RGGWCCY	1 cut(s)	875
Psp6I	CCWGG	1 cut(s)	813
PspGI	CCWGG	1 cut(s)	813
PspN4I	GGNNCC	3 cut(s)	39, 876, 1027
PspPI	GGNCC	1 cut(s)	875
PspPPI	RGGWCCY	1 cut(s)	875
PstNI	CAGNNNCTG	1 cut(s)	713
PsuI	RGATCY	2 cut(s)	109, 550
RsaI	GTAC	2 cut(s)	153, 533
RsaNI	GTAC	2 cut(s)	152, 532
RseI	CAYNNNNRTG	2 cut(s)	90, 708
SacI	GAGCTC	1 cut(s)	29
SaqAI	TTAA	3 cut(s)	71, 272, 696
SatI	GCNGC	2 cut(s)	368, 371
Sau3AI	GATC	5 cut(s)	103, 109, 159, 453, 550
Sau96I	GGNCC	1 cut(s)	875
SchI	GAGTC	1 cut(s)	628
ScrFI	CCNGG	1 cut(s)	815
SduI	GDGCHC	1 cut(s)	29
SetI	ASST	9 cut(s)	21, 29, 57, 148, 222, 576, 684, 891, 931
SfaNI	GCATC	3 cut(s)	589, 700, 805
SfcI	CTRYAG	1 cut(s)	75
SinI	GGWCC	1 cut(s)	875
SmiMI	CAYNNNNRTG	2 cut(s)	90, 708
SmlI	CTYRAG	1 cut(s)	147
SmoI	CTYRAG	1 cut(s)	147
Sse9I	AATT	4 cut(s)	8, 326, 378, 857
SseBI	AGGCCT	1 cut(s)	756
SsiI	CCGC	1 cut(s)	539
SspI	AATATT	1 cut(s)	499
SspMI	CTAG	3 cut(s)	20, 545, 1036
SstI	GAGCTC	1 cut(s)	29
StuI	AGGCCT	1 cut(s)	756
StyD4I	CCNGG	1 cut(s)	813
StyI	CCWWGG	1 cut(s)	628
TaaI	ACNGT	5 cut(s)	79, 360, 785, 808, 851
TaqI	TCGA	2 cut(s)	162, 969
TasI	AATT	4 cut(s)	8, 326, 378, 857
TatI	WGTACW	1 cut(s)	531
TfiI	GAWTC	4 cut(s)	260, 319, 565, 1000
Tru1I	TTAA	3 cut(s)	71, 272, 696
Tru9I	TTAA	3 cut(s)	71, 272, 696
TscAI	CASTG	3 cut(s)	162, 594, 813
TseFI	GTSAC	1 cut(s)	766
TseI	GCWGC	2 cut(s)	367, 370
Tsp45I	GTSAC	1 cut(s)	766
TspDTI	ATGAA	5 cut(s)	109, 182, 665, 759, 972
TspGWI	ACGGA	1 cut(s)	253
TspRI	CASTG	3 cut(s)	162, 594, 813
VpaK11BI	GGWCC	1 cut(s)	875
XapI	RAATTY	1 cut(s)	326
XmnI	GAANNNNTTC	1 cut(s)	756
XspI	CTAG	3 cut(s)	20, 545, 1036

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.