Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0000963

Physical Location & Seq

Genomic Coordinates Forward (+)

38898338 .. 38903210

Gene Structure 4873 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1272400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1098 bp

ATGGAAAAAACGACATCTGCCAGGAAGAGCTTGTACGGTGGTTCTCTTCATGTTCCTAGTGTTCAAGAATTCGTAAAGAAGCCAATACTCACAATCCCACCAAGATACTTACAGCCTCATTATCAAGATGGTGAGCATGAGATGATCATCAATTCTGATCATCATGCTCATCAAATACCATCCATTGACATGCAGAAATTGCTTTCCCAACAATCAACCAATTCTGAAGCTGAACTAGCCAGGCTCCATTTTGCTTGCAAAGAGTGGGGTTTTTTCCAGTTGGTAAACCATGGTGTGAGCTCTTCCGTGTTGGAGAAAATGAAGACAGAAACTCAAGAGTTCTTCAACCTACCCATGGAAGAGAAGAAAAAGTTCTCGCAACAGCCAGGAGACATTGAAGGTTATGGACAAGCATTTGTAGTTTCTGAAGACCAAAAACTCGATTGGTCTGACATGTTCTTCTTGACCACCCTTCCTGTCCAATTGAGGAAGCCTCACCTACTCCCAAAGCTCCCTTCTCCTTTCAGAGAGACTTTAGAAACTTACTCATCGGAACTGAAAAACCTAGCCATGACTATCCTATCACAAATGGAAAAAGCTTTGCAGATGGAAGCCAAGGAAGTAACCAACATATTCGAAGGTGGATTGCAAGCGGTGAGGGTCAACTATTACCCTCCATGTCCTCAGCCGGGGAAAGTCCTCGGTCTGCCCCCTCACTCTGATGGTGGAGCTCTCACCATCCTCCTACAAGTCAATGAAATGGATGGCCTCCAAGTGAAGAAAGATGGGATTTGGGTTCCAGTGAAGCCACTACCGGATGCCTTTATTGTGAATATTGGAGACGTTCTAGAGATTATAACAAATGGGACGTATCGTAGCATTGAACATCGTGCAACTGTGAACTCTGAAAAAGAAAGGCTCTCAATCGCCACATTTTACAACCCCAGAGTTGATGGTGAAATCGGCCCGGCCTCTAGCTTGATCACTAAGCAAACACCTGCAGCATTTAAAAGGCTGAGTGTTGAAGAATATTTCAAAGGTTTTTTTGAACGTAAGCTTCATGAAAAGTCTTTTCTTGACGAATTGCGGATTAAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0007275 GO:0007568 GO:0008150 GO:0008152 GO:0010150 GO:0016491 GO:0016679 GO:0016682 GO:0032501 GO:0032502 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055114 GO:0090693 GO:0099402

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

2OG-FeII_Oxy DIOX_N

Protein Analysis

366

Amino Acids

41.44

Weight (kDa)

6.2

Isoelectric Point (pI)

45.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DIOX_N	PF14226	59 - 169	3.6e-33	non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy	PF03171	217 - 315	8.6e-35	2OG-Fe(II) oxygenase superfamily

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000963)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17010 AT1G17020 AT4G25300 AT4G25300 AT4G25310 AT4G25310
fragaria_vesca	FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06511 FvH4_3g06512 FvH4_3g06513 FvH4_3g06513 FvH4_3g06513 FvH4_3g17470
malus_domestica	MD03G1251500.v1.1 MD11G1271400.v1.1 MD11G1272000.v1.1 MD11G1272100.v1.1 MD11G1272300.v1.1 MD11G1272400.v1.1 MD11G1272500.v1.1
prunus_persica	Prupe.4G154800_v2.0.a1 Prupe.4G154800_v2.0.a1 Prupe.4G154900_v2.0.a1 Prupe.4G155000_v2.0.a1 Prupe.4G155100_v2.0.a1
pyrus_communis	pycom11g23990 pycom11g24060 pycom11g24070
rosa_chinensis	RchiOBHm_Chr5g0012661 RchiOBHm_Chr5g0028991 RchiOBHm_Chr5g0029011
rosa_laevigata	RLG00000031946 RLG00000033134
rosa_multiflora	Rmu_sc0003577.1_g000001 Rmu_sc0009042.1_g000002 Rmu_sc0011332.1_g000001 Rmu_sc0011896.1_g000003
rosa_roxburghii	Rroxscaffold_1G00050860 Rroxscaffold_1G00050890 Rroxscaffold_1G00063940
rosa_rugosa	Rorug05G0003500 Rorug05G0112200
rosa_samantha	Rh5AG097300 Rh5AG204300 Rh5AG204400 Rh5BG093700 Rh5BG202600 Rh5BG202700 Rh5CG223500 Rh5CG223600 Rh5DG092800 Rh5DG206300 Rh5DG206400
rosa_wichuraiana	Rw5G008530 Rw5G018520 Rw5G018530

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	857
AarI	CACCTGC	1 cut(s)	1006
Acc36I	ACCTGC	1 cut(s)	1006
AciI	CCGC	2 cut(s)	653, 1087
AcsI	RAATTY	1 cut(s)	68
AcuI	CTGAAG	2 cut(s)	246, 447
AfaI	GTAC	1 cut(s)	35
AfiI	CCNNNNNNNGG	2 cut(s)	355, 689
AflIII	ACRYGT	1 cut(s)	453
AgsI	TTSAA	7 cut(s)	65, 346, 398, 884, 1025, 1036, 1049
AjnI	CCWGG	3 cut(s)	20, 239, 385
AjuI	GAANNNNNNNTTGG	2 cut(s)	499, 531
AluBI	AGCT	8 cut(s)	30, 230, 300, 511, 599, 731, 978, 1057
AluI	AGCT	8 cut(s)	30, 230, 300, 511, 599, 731, 978, 1057
Alw21I	GWGCWC	2 cut(s)	302, 733
Alw26I	GTCTC	3 cut(s)	384, 524, 834
AoxI	GGCC	3 cut(s)	766, 964, 969
ApeKI	GCWGC	1 cut(s)	1001
ApoI	RAATTY	1 cut(s)	68
Asp700I	GAANNNNTTC	1 cut(s)	371
AspS9I	GGNCC	1 cut(s)	965
AsuC2I	CCSGG	2 cut(s)	690, 968
AsuHPI	GGTGA	5 cut(s)	143, 488, 667, 727, 968
AsuII	TTCGAA	1 cut(s)	636
BanII	GRGCYC	2 cut(s)	302, 733
BarI	GAAGNNNNNNTAC	2 cut(s)	17, 49
BbsI	GAAGAC	2 cut(s)	329, 435
Bbv12I	GWGCWC	2 cut(s)	302, 733
BbvCI	CCTCAGC	1 cut(s)	684
BbvI	GCAGC	1 cut(s)	1013
BccI	CCATC	8 cut(s)	122, 187, 601, 716, 746, 758, 779, 947
BciT130I	CCWGG	3 cut(s)	22, 241, 387
BclI	TGATCA	3 cut(s)	144, 157, 981
BcnI	CCSGG	2 cut(s)	690, 968
BcoDI	GTCTC	3 cut(s)	384, 524, 834
BfaI	CTAG	5 cut(s)	57, 236, 566, 848, 975
BfmI	CTRYAG	1 cut(s)	999
BfuAI	ACCTGC	1 cut(s)	1006
BisI	GCNGC	1 cut(s)	1002
BlsI	GCNGC	1 cut(s)	1003
Bme1390I	CCNGG	5 cut(s)	22, 241, 387, 690, 968
BmgT120I	GGNCC	1 cut(s)	965
BmiI	GGNNCC	2 cut(s)	245, 798
BmrFI	CCNGG	5 cut(s)	22, 241, 387, 690, 968
BmsI	GCATC	1 cut(s)	808
BpiI	GAAGAC	2 cut(s)	329, 435
BplI	GAGNNNNNCTC	2 cut(s)	478, 510
Bpu10I	CCTNAGC	1 cut(s)	684
Bpu14I	TTCGAA	1 cut(s)	636
BpuEI	CTTGAG	1 cut(s)	318
BpuMI	CCSGG	2 cut(s)	690, 968
BsaBI	GATNNNNATC	1 cut(s)	146
BsaJI	CCNNGG	5 cut(s)	289, 354, 615, 689, 700
BsaWI	WCCGGW	1 cut(s)	814
Bsc4I	CCNNNNNNNGG	2 cut(s)	355, 689
Bse1I	ACTGG	2 cut(s)	277, 800
Bse8I	GATNNNNATC	1 cut(s)	146
BseBI	CCWGG	3 cut(s)	22, 241, 387
BseDI	CCNNGG	5 cut(s)	289, 354, 615, 689, 700
BseGI	GGATG	4 cut(s)	179, 738, 769, 823
BseJI	GATNNNNATC	1 cut(s)	146
BseLI	CCNNNNNNNGG	2 cut(s)	355, 689
BseMII	CTCAG	2 cut(s)	698, 1007
BseNI	ACTGG	2 cut(s)	277, 800
BseXI	GCAGC	1 cut(s)	1013
BshFI	GGCC	3 cut(s)	768, 966, 971
BsiHKAI	GWGCWC	2 cut(s)	302, 733
BsiSI	CCGG	3 cut(s)	689, 815, 968
BslFI	GGGAC	1 cut(s)	880
BslI	CCNNNNNNNGG	2 cut(s)	355, 689
BsmAI	GTCTC	3 cut(s)	384, 524, 834
BsmBI	CGTCTC	1 cut(s)	834
BsmFI	GGGAC	1 cut(s)	880
BsnI	GGCC	3 cut(s)	768, 966, 971
Bsp119I	TTCGAA	1 cut(s)	636
Bsp1286I	GDGCHC	2 cut(s)	302, 733
Bsp143I	GATC	3 cut(s)	144, 157, 981
Bsp19I	CCATGG	2 cut(s)	289, 354
BspACI	CCGC	2 cut(s)	653, 1087
BspANI	GGCC	3 cut(s)	768, 966, 971
BspCNI	CTCAG	2 cut(s)	697, 1008
BspHI	TCATGA	1 cut(s)	1060
BspLI	GGNNCC	2 cut(s)	245, 798
BspMAI	CTGCAG	1 cut(s)	1003
BspMI	ACCTGC	1 cut(s)	1006
BspQI	GCTCTTC	2 cut(s)	20, 307
BspT104I	TTCGAA	1 cut(s)	636
BsrI	ACTGG	2 cut(s)	277, 800
BssECI	CCNNGG	5 cut(s)	289, 354, 615, 689, 700
BssMI	GATC	3 cut(s)	144, 157, 981
BssT1I	CCWWGG	3 cut(s)	289, 354, 615
Bst2UI	CCWGG	3 cut(s)	22, 241, 387
Bst4CI	ACNGT	2 cut(s)	38, 898
Bst6I	CTCTTC	4 cut(s)	20, 51, 307, 354
BstAPI	GCANNNNNTGC	1 cut(s)	199
BstBI	TTCGAA	1 cut(s)	636
BstC8I	GCNNGC	2 cut(s)	256, 651
BstDEI	CTNAG	3 cut(s)	684, 987, 1016
BstDSI	CCRYGG	2 cut(s)	289, 354
BstF5I	GGATG	4 cut(s)	179, 738, 769, 823
BstKTI	GATC	3 cut(s)	147, 160, 984
BstMAI	GTCTC	3 cut(s)	384, 524, 834
BstMBI	GATC	3 cut(s)	144, 157, 981
BstMWI	GCNNNNNNNGC	2 cut(s)	199, 236
BstNI	CCWGG	3 cut(s)	22, 241, 387
BstNSI	RCATGY	2 cut(s)	193, 457
BstSCI	CCNGG	5 cut(s)	20, 239, 385, 688, 966
BstSFI	CTRYAG	1 cut(s)	999
BstV1I	GCAGC	1 cut(s)	1013
BstV2I	GAAGAC	2 cut(s)	329, 435
BsuRI	GGCC	3 cut(s)	768, 966, 971
BtgI	CCRYGG	2 cut(s)	289, 354
BtsCI	GGATG	4 cut(s)	179, 738, 769, 823
BtsIMutI	CAGTG	1 cut(s)	807
BveI	ACCTGC	1 cut(s)	1006
Cac8I	GCNNGC	2 cut(s)	256, 651
CciI	TCATGA	1 cut(s)	1060
Cfr13I	GGNCC	1 cut(s)	965
Csp6I	GTAC	1 cut(s)	34
CviQI	GTAC	1 cut(s)	34
DdeI	CTNAG	3 cut(s)	684, 987, 1016
DpnI	GATC	3 cut(s)	146, 159, 983
DpnII	GATC	3 cut(s)	144, 157, 981
DraI	TTTAAA	1 cut(s)	1009
Eam1104I	CTCTTC	4 cut(s)	20, 51, 307, 354
EarI	CTCTTC	4 cut(s)	20, 51, 307, 354
Ecl136II	GAGCTC	2 cut(s)	300, 731
Eco130I	CCWWGG	3 cut(s)	289, 354, 615
Eco24I	GRGCYC	2 cut(s)	302, 733
Eco53kI	GAGCTC	2 cut(s)	300, 731
Eco57I	CTGAAG	2 cut(s)	246, 447
EcoICRI	GAGCTC	2 cut(s)	300, 731
EcoRI	GAATTC	1 cut(s)	68
EcoRII	CCWGG	3 cut(s)	20, 239, 385
EcoT14I	CCWWGG	3 cut(s)	289, 354, 615
EcoT38I	GRGCYC	2 cut(s)	302, 733
ErhI	CCWWGG	3 cut(s)	289, 354, 615
Esp3I	CGTCTC	1 cut(s)	834
FaqI	GGGAC	1 cut(s)	880
FbaI	TGATCA	3 cut(s)	144, 157, 981
Fnu4HI	GCNGC	1 cut(s)	1002
FokI	GGATG	4 cut(s)	166, 725, 776, 830
FriOI	GRGCYC	2 cut(s)	302, 733
Fsp4HI	GCNGC	1 cut(s)	1002
FspBI	CTAG	5 cut(s)	57, 236, 566, 848, 975
GluI	GCNGC	1 cut(s)	1002
HaeIII	GGCC	3 cut(s)	768, 966, 971
HapII	CCGG	3 cut(s)	689, 815, 968
HincII	GTYRAC	1 cut(s)	664
HindII	GTYRAC	1 cut(s)	664
HindIII	AAGCTT	2 cut(s)	597, 1055
HpaII	CCGG	3 cut(s)	689, 815, 968
HphI	GGTGA	5 cut(s)	143, 488, 667, 727, 968
Hpy166II	GTNNAC	3 cut(s)	286, 664, 901
Hpy188I	TCNGA	8 cut(s)	157, 226, 427, 451, 527, 553, 721, 907
Hpy188III	TCNNGA	7 cut(s)	65, 125, 335, 463, 848, 1061, 1076
Hpy8I	GTNNAC	3 cut(s)	286, 664, 901
HpyAV	CCTTC	4 cut(s)	392, 482, 525, 632
HpyCH4III	ACNGT	2 cut(s)	38, 898
HpyCH4IV	ACGT	3 cut(s)	843, 869, 1051
HpyCH4V	TGCA	6 cut(s)	193, 258, 604, 649, 893, 1001
HpyF10VI	GCNNNNNNNGC	2 cut(s)	199, 236
HpyF3I	CTNAG	3 cut(s)	684, 987, 1016
HpySE526I	ACGT	3 cut(s)	843, 869, 1051
Ksp22I	TGATCA	3 cut(s)	144, 157, 981
Kzo9I	GATC	3 cut(s)	144, 157, 981
LguI	GCTCTTC	2 cut(s)	20, 307
LmnI	GCTCC	3 cut(s)	249, 516, 728
Lsp1109I	GCAGC	1 cut(s)	1013
LweI	GCATC	1 cut(s)	808
MaeI	CTAG	5 cut(s)	57, 236, 566, 848, 975
MaeII	ACGT	3 cut(s)	843, 869, 1051
MaeIII	GTNAC	1 cut(s)	622
MalI	GATC	3 cut(s)	146, 159, 983
MboI	GATC	3 cut(s)	144, 157, 981
MfeI	CAATTG	1 cut(s)	482
MhlI	GDGCHC	2 cut(s)	302, 733
MluCI	AATT	6 cut(s)	68, 151, 197, 220, 482, 1082
MmeI	TCCRAC	1 cut(s)	291
MroXI	GAANNNNTTC	1 cut(s)	371
MseI	TTAA	2 cut(s)	1008, 1092
MslI	CAYNNNNRTG	2 cut(s)	188, 720
MspI	CCGG	3 cut(s)	689, 815, 968
MspR9I	CCNGG	5 cut(s)	22, 241, 387, 690, 968
MunI	CAATTG	1 cut(s)	482
MvaI	CCWGG	3 cut(s)	22, 241, 387
MwoI	GCNNNNNNNGC	2 cut(s)	199, 236
NciI	CCSGG	2 cut(s)	690, 968
NcoI	CCATGG	2 cut(s)	289, 354
NdeII	GATC	3 cut(s)	144, 157, 981
NlaIV	GGNNCC	2 cut(s)	245, 798
NspI	RCATGY	2 cut(s)	193, 457
NspV	TTCGAA	1 cut(s)	636
PagI	TCATGA	1 cut(s)	1060
PaqCI	CACCTGC	1 cut(s)	1006
PciI	ACATGT	1 cut(s)	453
PciSI	GCTCTTC	2 cut(s)	20, 307
PdmI	GAANNNNTTC	1 cut(s)	371
PkrI	GCNGC	1 cut(s)	1003
PscI	ACATGT	1 cut(s)	453
PsiI	TTATAA	1 cut(s)	857
Psp124BI	GAGCTC	2 cut(s)	302, 733
Psp6I	CCWGG	3 cut(s)	20, 239, 385
PspGI	CCWGG	3 cut(s)	20, 239, 385
PspN4I	GGNNCC	2 cut(s)	245, 798
PspPI	GGNCC	1 cut(s)	965
PstI	CTGCAG	1 cut(s)	1003
RsaI	GTAC	1 cut(s)	35
RsaNI	GTAC	1 cut(s)	34
RseI	CAYNNNNRTG	2 cut(s)	188, 720
SacI	GAGCTC	2 cut(s)	302, 733
SapI	GCTCTTC	2 cut(s)	20, 307
SaqAI	TTAA	2 cut(s)	1008, 1092
SatI	GCNGC	1 cut(s)	1002
Sau3AI	GATC	3 cut(s)	144, 157, 981
Sau96I	GGNCC	1 cut(s)	965
ScrFI	CCNGG	5 cut(s)	22, 241, 387, 690, 968
SduI	GDGCHC	2 cut(s)	302, 733
SfaNI	GCATC	1 cut(s)	808
SfcI	CTRYAG	1 cut(s)	999
SfuI	TTCGAA	1 cut(s)	636
SmiMI	CAYNNNNRTG	2 cut(s)	188, 720
SmlI	CTYRAG	1 cut(s)	333
SmoI	CTYRAG	1 cut(s)	333
Sse9I	AATT	6 cut(s)	68, 151, 197, 220, 482, 1082
SsiI	CCGC	2 cut(s)	653, 1087
SspI	AATATT	2 cut(s)	835, 1031
SspMI	CTAG	5 cut(s)	57, 236, 566, 848, 975
SstI	GAGCTC	2 cut(s)	302, 733
StyD4I	CCNGG	5 cut(s)	20, 239, 385, 688, 966
StyI	CCWWGG	3 cut(s)	289, 354, 615
TaaI	ACNGT	2 cut(s)	38, 898
TaiI	ACGT	3 cut(s)	846, 872, 1054
TaqI	TCGA	2 cut(s)	441, 636
TaqII	GACCGA	1 cut(s)	692
TasI	AATT	6 cut(s)	68, 151, 197, 220, 482, 1082
Tru1I	TTAA	2 cut(s)	1008, 1092
Tru9I	TTAA	2 cut(s)	1008, 1092
TscAI	CASTG	1 cut(s)	807
TseI	GCWGC	1 cut(s)	1001
TspDTI	ATGAA	5 cut(s)	38, 335, 771, 1049, 1077
TspGWI	ACGGA	1 cut(s)	295
TspRI	CASTG	1 cut(s)	807
XapI	RAATTY	1 cut(s)	68
XbaI	TCTAGA	1 cut(s)	847
XceI	RCATGY	2 cut(s)	193, 457
XmnI	GAANNNNTTC	1 cut(s)	371
XspI	CTAG	5 cut(s)	57, 236, 566, 848, 975

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.