Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0001218

Physical Location & Seq

Genomic Coordinates Forward (+)

22709235 .. 22712573

Gene Structure 3339 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1138800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 924 bp

ATGGCGAACGACAAGTCGGTGATGGCAGTAATCAGAGCCGCGAGGCCGAACTTCCGCAACAACGACGACAAGGTCGTTTTCGCCGTTCACGCCTCCTTCCTCGCCGCTGGCTACGTCCTCACCGCCGCCGGTCCCCCTGCCTTCTCCGAATCCGCTCTCTCCTCATCTTCTACGGATGAAGTGGGCATGGAGCAGTGGAACGAGCTCGACGGCGAGTACGCATTCGTCTATATGAACCCAGAAAAGGGCTCGAAGAAGGTGCTGGTTAAGTGCCTTACGATGAACGGCAAGTTGCTTGTGGATGCACTGGCTGATGGGAGCTCTCAGCCCGTCCATCTCGAAATCGATGTTGGTGATTATGTTGGAGAGGATGGGGGCAGCAATTACTCCACACAGTTCAAGAATTTAGAGAATCTGGTGAAGAAATTGGATACTGAAGTTCTGTCAAAATTATATGGGTCTGGATCGTCCACATCCGGTTCTTCAATTAACCCTGTAAGTTCAGAAAAGAGTGAGAGATCAACAACTGAGTCTGTTTCTGGACCTTCACGCTTTCACACTGGACCTTCAGCCCCTTACACTGATCCTTCAGGAATTGCGTATCCTCCTGTCATGCCTTATGGTGGTAGTGATGTTCTTCCTGGGCCTGGTGCTGGAGTCTACCCTTCAAGGGGTGATGGTAGTGGAAGCATGCTTGTAGGACCCCATGACCCTCGTTGGTTTGGTGGCGTTGGAGACCCTGGGTTTAGTTTTGATCCCTCTAGGCCAGCAGGTGTTCCACCAGGTGCTCGTTTTGATCCCTATGGGCCCCCAGGTGTTCCAGGTTTTGAGCCTAATCGATTTGCAAGGAATCCACGGAGGCCGCCGGGAGGTGGCACTCATCCAGACCTGGAGCATTTCGGGAGCGGCTCAGATTTCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005783 GO:0005886 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009719 GO:0009725 GO:0009733 GO:0009892 GO:0009894 GO:0009895 GO:0009987 GO:0010033 GO:0010605 GO:0012505 GO:0016020 GO:0019222 GO:0019538 GO:0019941 GO:0030162 GO:0030163 GO:0031323 GO:0031324 GO:0031329 GO:0031330 GO:0032268 GO:0032269 GO:0032870 GO:0042176 GO:0042177 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0045861 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0050896 GO:0051171 GO:0051172 GO:0051246 GO:0051248 GO:0051603 GO:0051716 GO:0060255 GO:0061136 GO:0065007 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071704 GO:0071944 GO:0080090 GO:1901564 GO:1901565 GO:1901575 GO:1901799 GO:1903050 GO:1903051 GO:1903362 GO:1903363

KEGG Pathways

Metabolic & Signaling

ko03050 map03050

Pfam Domains

Protein Families

PI31_Prot_C PI31_Prot_N

Protein Analysis

308

Amino Acids

32.21

Weight (kDa)

5.07

Isoelectric Point (pI)

40.33

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PI31_Prot_N	PF11566	15 - 153	8.9e-26	PI31 proteasome regulator N-terminal

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001218)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G48530 AT3G53970 AT3G53970
fragaria_vesca	FvH4_4g10740 FvH4_4g10740 FvH4_6g12740
malus_domestica	MD04G1138800.v1.1 MD12G1154600.v1.1
prunus_persica	Prupe.6G264400_v2.0.a1
pyrus_communis	pycom12g14680
rosa_chinensis	RchiOBHm_Chr3g0464661 RchiOBHm_Chr4g0406841 RchiOBHm_Chr4g0416021 RchiOBHm_Chr4g0419261 RchiOBHm_Chr5g0047111 RchiOBHm_Chr6g0273171
rosa_laevigata	RLG00000007821 RLG00000008752 RLG00000024677 RLG00000027129
rosa_multiflora	Rmu_sc0002031.1_g000004 Rmu_sc0002316.1_g000083 Rmu_sc0004932.1_g000003 Rmu_sc0008087.1_g000005 Rmu_sc0030142.1_g000001 Rmu_sc0042118.1_g000001
rosa_roxburghii	Rroxscaffold_1G00034330 Rroxscaffold_5G00362230 Rroxscaffold_6G00415480 Rroxscaffold_6G00417790
rosa_rugosa	Rorug03G0073600 Rorug04G0074500 Rorug04G0157800
rosa_samantha	Rh2AG335700 Rh2AG421800 Rh2CG188400 Rh2CG409000 Rh3BG135900 Rh3CG138500 Rh3DG137500 Rh4AG141300 Rh4BG137800 Rh4CG148000 Rh4CG232300 Rh4DG135100 Rh5AG387600 Rh5BG419600 Rh5CG456900 Rh5CG457000 Rh5DG415700 Rh6DG223400
rosa_wichuraiana	Rw3G011130 Rw4G011500 Rw4G018780 Rw5G019600 Rw6G002620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	761
AasI	GACNNNNNNGTC	1 cut(s)	71
Acc36I	ACCTGC	1 cut(s)	761
AccBSI	CCGCTC	2 cut(s)	155, 906
AccI	GTMKAC	1 cut(s)	660
AccII	CGCG	1 cut(s)	41
AciI	CCGC	8 cut(s)	39, 55, 105, 123, 126, 153, 863, 906
AclWI	GGATC	4 cut(s)	472, 578, 749, 791
AcsI	RAATTY	1 cut(s)	403
AcuI	CTGAAG	3 cut(s)	456, 552, 573
AdeI	CACNNNGTG	1 cut(s)	785
AfaI	GTAC	1 cut(s)	218
AfiI	CCNNNNNNNGG	9 cut(s)	244, 245, 623, 647, 653, 670, 671, 869, 872
AgsI	TTSAA	3 cut(s)	400, 486, 669
AjnI	CCWGG	7 cut(s)	640, 646, 739, 781, 811, 820, 888
AjuI	GAANNNNNNNTTGG	2 cut(s)	333, 365
AluBI	AGCT	2 cut(s)	205, 321
AluI	AGCT	2 cut(s)	205, 321
Alw21I	GWGCWC	3 cut(s)	207, 323, 790
Alw26I	GTCTC	1 cut(s)	729
AlwI	GGATC	4 cut(s)	472, 578, 749, 791
AoxI	GGCC	5 cut(s)	44, 644, 764, 806, 860
ApaI	GGGCCC	1 cut(s)	810
ApeKI	GCWGC	1 cut(s)	378
ApoI	RAATTY	1 cut(s)	403
ArsI	GACNNNNNNTTYG	1 cut(s)	31
AspS9I	GGNCC	7 cut(s)	131, 542, 563, 644, 701, 806, 807
AsuC2I	CCSGG	1 cut(s)	867
AsuHPI	GGTGA	5 cut(s)	31, 112, 365, 430, 686
AvaII	GGWCC	4 cut(s)	131, 542, 563, 701
BaeGI	GKGCMC	1 cut(s)	810
BaeI	ACNNNNGTAYC	2 cut(s)	423, 456
BanII	GRGCYC	4 cut(s)	207, 251, 323, 810
Bbv12I	GWGCWC	3 cut(s)	207, 323, 790
BbvI	GCAGC	1 cut(s)	390
BccI	CCATC	5 cut(s)	16, 308, 342, 365, 671
BceAI	ACGGC	3 cut(s)	68, 226, 301
BcgI	CGANNNNNNTGC	2 cut(s)	241, 275
BciT130I	CCWGG	7 cut(s)	642, 648, 741, 783, 813, 822, 890
BciVI	GTATCC	2 cut(s)	424, 612
BcnI	CCSGG	1 cut(s)	867
BcoDI	GTCTC	1 cut(s)	729
BfaI	CTAG	1 cut(s)	762
BfuAI	ACCTGC	1 cut(s)	761
BfuI	GTATCC	2 cut(s)	424, 612
BisI	GCNGC	6 cut(s)	39, 105, 126, 379, 863, 907
BlsI	GCNGC	6 cut(s)	40, 106, 127, 380, 864, 908
Bme1390I	CCNGG	8 cut(s)	642, 648, 741, 783, 813, 822, 867, 890
Bme18I	GGWCC	4 cut(s)	131, 542, 563, 701
BmgT120I	GGNCC	7 cut(s)	131, 542, 563, 644, 701, 806, 807
BmiI	GGNNCC	4 cut(s)	133, 703, 808, 809
BmrFI	CCNGG	8 cut(s)	642, 648, 741, 783, 813, 822, 867, 890
BmsI	GCATC	1 cut(s)	292
BpmI	CTGGAG	2 cut(s)	675, 911
BpuMI	CCSGG	1 cut(s)	867
Bsa29I	ATCGAT	2 cut(s)	345, 838
BsaI	GGTCTC	1 cut(s)	729
BsaJI	CCNNGG	5 cut(s)	641, 739, 740, 811, 854
BsaWI	WCCGGW	1 cut(s)	476
Bsc4I	CCNNNNNNNGG	9 cut(s)	244, 245, 623, 647, 653, 670, 671, 869, 872
Bse118I	RCCGGY	1 cut(s)	128
Bse1I	ACTGG	2 cut(s)	312, 565
BseBI	CCWGG	7 cut(s)	642, 648, 741, 783, 813, 822, 890
BseCI	ATCGAT	2 cut(s)	345, 838
BseDI	CCNNGG	5 cut(s)	641, 739, 740, 811, 854
BseGI	GGATG	5 cut(s)	181, 307, 376, 473, 880
BseLI	CCNNNNNNNGG	9 cut(s)	244, 245, 623, 647, 653, 670, 671, 869, 872
BseMII	CTCAG	3 cut(s)	338, 519, 924
BseNI	ACTGG	2 cut(s)	312, 565
BseRI	GAGGAG	1 cut(s)	151
BseSI	GKGCMC	1 cut(s)	810
BseXI	GCAGC	1 cut(s)	390
Bsh1236I	CGCG	1 cut(s)	41
BshFI	GGCC	5 cut(s)	46, 646, 766, 808, 862
BshVI	ATCGAT	2 cut(s)	345, 838
BsiHKAI	GWGCWC	3 cut(s)	207, 323, 790
BsiSI	CCGG	3 cut(s)	129, 477, 866
BslFI	GGGAC	1 cut(s)	117
BslI	CCNNNNNNNGG	9 cut(s)	244, 245, 623, 647, 653, 670, 671, 869, 872
BsmAI	GTCTC	1 cut(s)	729
BsmFI	GGGAC	1 cut(s)	117
BsmI	GAATGC	1 cut(s)	221
BsnI	GGCC	5 cut(s)	46, 646, 766, 808, 862
Bso31I	GGTCTC	1 cut(s)	729
Bsp120I	GGGCCC	1 cut(s)	806
Bsp1286I	GDGCHC	5 cut(s)	207, 251, 323, 790, 810
Bsp143I	GATC	5 cut(s)	464, 518, 583, 754, 796
BspACI	CCGC	8 cut(s)	39, 55, 105, 123, 126, 153, 863, 906
BspANI	GGCC	5 cut(s)	46, 646, 766, 808, 862
BspCNI	CTCAG	3 cut(s)	337, 520, 923
BspDI	ATCGAT	2 cut(s)	345, 838
BspFNI	CGCG	1 cut(s)	41
BspLI	GGNNCC	4 cut(s)	133, 703, 808, 809
BspMI	ACCTGC	1 cut(s)	761
BspPI	GGATC	4 cut(s)	472, 578, 749, 791
BspTNI	GGTCTC	1 cut(s)	729
BsrBI	CCGCTC	2 cut(s)	155, 906
BsrFI	RCCGGY	1 cut(s)	128
BsrI	ACTGG	2 cut(s)	312, 565
BssAI	RCCGGY	1 cut(s)	128
BssECI	CCNNGG	5 cut(s)	641, 739, 740, 811, 854
BssMI	GATC	5 cut(s)	464, 518, 583, 754, 796
Bst2UI	CCWGG	7 cut(s)	642, 648, 741, 783, 813, 822, 890
Bst4CI	ACNGT	1 cut(s)	396
BstC8I	GCNNGC	3 cut(s)	109, 692, 768
BstDEI	CTNAG	3 cut(s)	324, 528, 910
BstDSI	CCRYGG	1 cut(s)	854
BstF5I	GGATG	5 cut(s)	181, 307, 376, 473, 880
BstFNI	CGCG	1 cut(s)	41
BstKTI	GATC	5 cut(s)	467, 521, 586, 757, 799
BstMAI	GTCTC	1 cut(s)	729
BstMBI	GATC	5 cut(s)	464, 518, 583, 754, 796
BstMWI	GCNNNNNNNGC	1 cut(s)	89
BstNI	CCWGG	7 cut(s)	642, 648, 741, 783, 813, 822, 890
BstNSI	RCATGY	1 cut(s)	694
BstSCI	CCNGG	8 cut(s)	640, 646, 739, 781, 811, 820, 865, 888
BstSLI	GKGCMC	1 cut(s)	810
BstUI	CGCG	1 cut(s)	41
BstV1I	GCAGC	1 cut(s)	390
Bsu15I	ATCGAT	2 cut(s)	345, 838
BsuI	GTATCC	2 cut(s)	424, 612
BsuRI	GGCC	5 cut(s)	46, 646, 766, 808, 862
BsuTUI	ATCGAT	2 cut(s)	345, 838
BtgI	CCRYGG	1 cut(s)	854
BtsCI	GGATG	5 cut(s)	181, 307, 376, 473, 880
BtsI	GCAGTG	1 cut(s)	200
BtsIMutI	CAGTG	4 cut(s)	200, 305, 558, 579
BveI	ACCTGC	1 cut(s)	761
Cac8I	GCNNGC	3 cut(s)	109, 692, 768
Cfr10I	RCCGGY	1 cut(s)	128
Cfr13I	GGNCC	7 cut(s)	131, 542, 563, 644, 701, 806, 807
ClaI	ATCGAT	2 cut(s)	345, 838
CsiI	ACCWGGT	1 cut(s)	781
Csp6I	GTAC	1 cut(s)	217
CviAII	CATG	4 cut(s)	187, 613, 691, 707
CviQI	GTAC	1 cut(s)	217
DdeI	CTNAG	3 cut(s)	324, 528, 910
DpnI	GATC	5 cut(s)	466, 520, 585, 756, 798
DpnII	GATC	5 cut(s)	464, 518, 583, 754, 796
DraIII	CACNNNGTG	1 cut(s)	785
DrdI	GACNNNNNNGTC	1 cut(s)	71
DseDI	GACNNNNNNGTC	1 cut(s)	71
Ecl136II	GAGCTC	2 cut(s)	205, 321
Eco24I	GRGCYC	4 cut(s)	207, 251, 323, 810
Eco31I	GGTCTC	1 cut(s)	729
Eco47I	GGWCC	4 cut(s)	131, 542, 563, 701
Eco53kI	GAGCTC	2 cut(s)	205, 321
Eco57I	CTGAAG	3 cut(s)	456, 552, 573
EcoICRI	GAGCTC	2 cut(s)	205, 321
EcoO109I	RGGNCCY	2 cut(s)	701, 807
EcoRII	CCWGG	7 cut(s)	640, 646, 739, 781, 811, 820, 888
EcoT38I	GRGCYC	4 cut(s)	207, 251, 323, 810
FaeI	CATG	4 cut(s)	190, 616, 694, 710
FaqI	GGGAC	1 cut(s)	117
FatI	CATG	4 cut(s)	186, 612, 690, 706
FblI	GTMKAC	1 cut(s)	660
Fnu4HI	GCNGC	6 cut(s)	39, 105, 126, 379, 863, 907
FokI	GGATG	5 cut(s)	188, 314, 383, 460, 867
FriOI	GRGCYC	4 cut(s)	207, 251, 323, 810
Fsp4HI	GCNGC	6 cut(s)	39, 105, 126, 379, 863, 907
FspBI	CTAG	1 cut(s)	762
GluI	GCNGC	6 cut(s)	39, 105, 126, 379, 863, 907
GsuI	CTGGAG	2 cut(s)	675, 911
HaeIII	GGCC	5 cut(s)	46, 646, 766, 808, 862
HapII	CCGG	3 cut(s)	129, 477, 866
Hin1II	CATG	4 cut(s)	190, 616, 694, 710
HinfI	GANTC	5 cut(s)	149, 412, 530, 657, 850
HpaII	CCGG	3 cut(s)	129, 477, 866
HphI	GGTGA	5 cut(s)	31, 112, 365, 430, 686
Hpy166II	GTNNAC	3 cut(s)	88, 471, 661
Hpy188I	TCNGA	4 cut(s)	35, 148, 505, 913
Hpy188III	TCNNGA	7 cut(s)	338, 400, 462, 540, 591, 884, 901
Hpy8I	GTNNAC	3 cut(s)	88, 471, 661
Hpy99I	CGWCG	2 cut(s)	68, 212
HpyAV	CCTTC	7 cut(s)	106, 151, 250, 555, 576, 597, 675
HpyCH4III	ACNGT	1 cut(s)	396
HpyCH4IV	ACGT	1 cut(s)	114
HpyCH4V	TGCA	2 cut(s)	305, 845
HpyF10VI	GCNNNNNNNGC	1 cut(s)	89
HpyF3I	CTNAG	3 cut(s)	324, 528, 910
HpySE526I	ACGT	1 cut(s)	114
Hsp92II	CATG	4 cut(s)	190, 616, 694, 710
Kzo9I	GATC	5 cut(s)	464, 518, 583, 754, 796
LmnI	GCTCC	4 cut(s)	190, 318, 892, 903
Lsp1109I	GCAGC	1 cut(s)	390
LweI	GCATC	1 cut(s)	292
MabI	ACCWGGT	1 cut(s)	781
MaeI	CTAG	1 cut(s)	762
MaeII	ACGT	1 cut(s)	114
MalI	GATC	5 cut(s)	466, 520, 585, 756, 798
MbiI	CCGCTC	2 cut(s)	155, 906
MboI	GATC	5 cut(s)	464, 518, 583, 754, 796
MboII	GAAGA	5 cut(s)	159, 265, 433, 474, 629
MhlI	GDGCHC	5 cut(s)	207, 251, 323, 790, 810
MluCI	AATT	6 cut(s)	382, 403, 425, 449, 486, 594
MlyI	GAGTC	2 cut(s)	539, 666
MmeI	TCCRAC	2 cut(s)	343, 712
MseI	TTAA	2 cut(s)	267, 489
MspA1I	CMGCKG	1 cut(s)	107
MspI	CCGG	3 cut(s)	129, 477, 866
MspR9I	CCNGG	8 cut(s)	642, 648, 741, 783, 813, 822, 867, 890
Mva1269I	GAATGC	1 cut(s)	221
MvaI	CCWGG	7 cut(s)	642, 648, 741, 783, 813, 822, 890
MvnI	CGCG	1 cut(s)	41
MwoI	GCNNNNNNNGC	1 cut(s)	89
NciI	CCSGG	1 cut(s)	867
NdeII	GATC	5 cut(s)	464, 518, 583, 754, 796
NlaIII	CATG	4 cut(s)	190, 616, 694, 710
NlaIV	GGNNCC	4 cut(s)	133, 703, 808, 809
NspI	RCATGY	1 cut(s)	694
PaeI	GCATGC	1 cut(s)	694
PaqCI	CACCTGC	1 cut(s)	761
PasI	CCCWGGG	1 cut(s)	740
PcsI	WCGNNNNNNNCGW	2 cut(s)	72, 81
PctI	GAATGC	1 cut(s)	221
PfeI	GAWTC	3 cut(s)	149, 412, 850
PflFI	GACNNNGTC	1 cut(s)	71
PkrI	GCNGC	6 cut(s)	40, 106, 127, 380, 864, 908
PleI	GAGTC	2 cut(s)	538, 665
PpsI	GAGTC	2 cut(s)	538, 665
PpuMI	RGGWCCY	1 cut(s)	701
Psp124BI	GAGCTC	2 cut(s)	207, 323
Psp5II	RGGWCCY	1 cut(s)	701
Psp6I	CCWGG	7 cut(s)	640, 646, 739, 781, 811, 820, 888
PspGI	CCWGG	7 cut(s)	640, 646, 739, 781, 811, 820, 888
PspN4I	GGNNCC	4 cut(s)	133, 703, 808, 809
PspOMI	GGGCCC	1 cut(s)	806
PspPI	GGNCC	7 cut(s)	131, 542, 563, 644, 701, 806, 807
PspPPI	RGGWCCY	1 cut(s)	701
PsyI	GACNNNGTC	1 cut(s)	71
RsaI	GTAC	1 cut(s)	218
RsaNI	GTAC	1 cut(s)	217
SacI	GAGCTC	2 cut(s)	207, 323
SaqAI	TTAA	2 cut(s)	267, 489
SatI	GCNGC	6 cut(s)	39, 105, 126, 379, 863, 907
Sau3AI	GATC	5 cut(s)	464, 518, 583, 754, 796
Sau96I	GGNCC	7 cut(s)	131, 542, 563, 644, 701, 806, 807
SchI	GAGTC	2 cut(s)	539, 666
ScrFI	CCNGG	8 cut(s)	642, 648, 741, 783, 813, 822, 867, 890
SduI	GDGCHC	5 cut(s)	207, 251, 323, 790, 810
SexAI	ACCWGGT	1 cut(s)	781
SfaNI	GCATC	1 cut(s)	292
SinI	GGWCC	4 cut(s)	131, 542, 563, 701
SphI	GCATGC	1 cut(s)	694
Sse9I	AATT	6 cut(s)	382, 403, 425, 449, 486, 594
SsiI	CCGC	8 cut(s)	39, 55, 105, 123, 126, 153, 863, 906
SspMI	CTAG	1 cut(s)	762
SstI	GAGCTC	2 cut(s)	207, 323
StyD4I	CCNGG	8 cut(s)	640, 646, 739, 781, 811, 820, 865, 888
TaaI	ACNGT	1 cut(s)	396
TaiI	ACGT	1 cut(s)	117
TaqI	TCGA	5 cut(s)	207, 251, 339, 345, 838
TasI	AATT	6 cut(s)	382, 403, 425, 449, 486, 594
TauI	GCSGC	5 cut(s)	41, 107, 128, 865, 909
TfiI	GAWTC	3 cut(s)	149, 412, 850
Tru1I	TTAA	2 cut(s)	267, 489
Tru9I	TTAA	2 cut(s)	267, 489
TscAI	CASTG	4 cut(s)	200, 312, 565, 586
TseI	GCWGC	1 cut(s)	378
TspDTI	ATGAA	4 cut(s)	192, 248, 296, 907
TspGWI	ACGGA	2 cut(s)	188, 871
TspRI	CASTG	4 cut(s)	200, 312, 565, 586
Tth111I	GACNNNGTC	1 cut(s)	71
VpaK11BI	GGWCC	4 cut(s)	131, 542, 563, 701
XapI	RAATTY	1 cut(s)	403
XceI	RCATGY	1 cut(s)	694
XmiI	GTMKAC	1 cut(s)	660
XspI	CTAG	1 cut(s)	762

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.