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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 159 |
| AcoI | YGGCCR | 1 cut(s) | 355 |
| AcsI | RAATTY | 1 cut(s) | 488 |
| AcuI | CTGAAG | 1 cut(s) | 89 |
| AfaI | GTAC | 4 cut(s) | 25, 40, 176, 270 |
| AgsI | TTSAA | 2 cut(s) | 104, 211 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 154 |
| AjiI | CACGTC | 1 cut(s) | 45 |
| AjnI | CCWGG | 1 cut(s) | 92 |
| AluBI | AGCT | 3 cut(s) | 114, 274, 352 |
| AluI | AGCT | 3 cut(s) | 114, 274, 352 |
| Alw26I | GTCTC | 1 cut(s) | 144 |
| AoxI | GGCC | 1 cut(s) | 355 |
| ApoI | RAATTY | 1 cut(s) | 488 |
| AspS9I | GGNCC | 1 cut(s) | 148 |
| AsuHPI | GGTGA | 2 cut(s) | 317, 441 |
| AvaII | GGWCC | 1 cut(s) | 148 |
| BalI | TGGCCA | 1 cut(s) | 357 |
| BanII | GRGCYC | 1 cut(s) | 125 |
| BccI | CCATC | 2 cut(s) | 157, 255 |
| BciT130I | CCWGG | 1 cut(s) | 94 |
| BcoDI | GTCTC | 1 cut(s) | 144 |
| BfaI | CTAG | 1 cut(s) | 291 |
| BfuAI | ACCTGC | 1 cut(s) | 159 |
| Bme1390I | CCNGG | 1 cut(s) | 94 |
| Bme18I | GGWCC | 1 cut(s) | 148 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 154 |
| BmgBI | CACGTC | 1 cut(s) | 45 |
| BmgT120I | GGNCC | 1 cut(s) | 148 |
| BmrFI | CCNGG | 1 cut(s) | 94 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 124, 156 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 158, 188 |
| Bse1I | ACTGG | 1 cut(s) | 358 |
| BseBI | CCWGG | 1 cut(s) | 94 |
| BseGI | GGATG | 3 cut(s) | 238, 266, 301 |
| BseNI | ACTGG | 1 cut(s) | 358 |
| BshFI | GGCC | 1 cut(s) | 357 |
| BsmAI | GTCTC | 1 cut(s) | 144 |
| BsnI | GGCC | 1 cut(s) | 357 |
| Bsp1286I | GDGCHC | 1 cut(s) | 125 |
| BspANI | GGCC | 1 cut(s) | 357 |
| BspMI | ACCTGC | 1 cut(s) | 159 |
| BsrI | ACTGG | 1 cut(s) | 358 |
| Bst2UI | CCWGG | 1 cut(s) | 94 |
| Bst4CI | ACNGT | 2 cut(s) | 77, 220 |
| Bst6I | CTCTTC | 2 cut(s) | 45, 245 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 394 |
| BstF5I | GGATG | 3 cut(s) | 238, 266, 301 |
| BstMAI | GTCTC | 1 cut(s) | 144 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 120, 271, 280, 394 |
| BstNI | CCWGG | 1 cut(s) | 94 |
| BstSCI | CCNGG | 1 cut(s) | 92 |
| BsuRI | GGCC | 1 cut(s) | 357 |
| BtrI | CACGTC | 1 cut(s) | 45 |
| BtsCI | GGATG | 3 cut(s) | 238, 266, 301 |
| BtsI | GCAGTG | 1 cut(s) | 436 |
| BtsIMutI | CAGTG | 3 cut(s) | 65, 365, 436 |
| BveI | ACCTGC | 1 cut(s) | 159 |
| Cfr13I | GGNCC | 1 cut(s) | 148 |
| Csp6I | GTAC | 4 cut(s) | 24, 39, 175, 269 |
| CviAII | CATG | 5 cut(s) | 20, 227, 346, 384, 394 |
| CviJI | RGCY | 9 cut(s) | 5, 114, 123, 265, 274, 352, 357, 373, 422 |
| CviKI_1 | RGCY | 9 cut(s) | 5, 114, 123, 265, 274, 352, 357, 373, 422 |
| CviQI | GTAC | 4 cut(s) | 24, 39, 175, 269 |
| DriI | GACNNNNNGTC | 1 cut(s) | 154 |
| EaeI | YGGCCR | 1 cut(s) | 355 |
| Eam1104I | CTCTTC | 2 cut(s) | 45, 245 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 154 |
| EarI | CTCTTC | 2 cut(s) | 45, 245 |
| Eco24I | GRGCYC | 1 cut(s) | 125 |
| Eco47I | GGWCC | 1 cut(s) | 148 |
| Eco57I | CTGAAG | 1 cut(s) | 89 |
| EcoRII | CCWGG | 1 cut(s) | 92 |
| EcoT38I | GRGCYC | 1 cut(s) | 125 |
| FaeI | CATG | 5 cut(s) | 23, 230, 349, 387, 397 |
| FaiI | YATR | 9 cut(s) | 21, 85, 87, 173, 228, 245, 347, 385, 395 |
| FatI | CATG | 5 cut(s) | 19, 226, 345, 383, 393 |
| FokI | GGATG | 3 cut(s) | 245, 273, 308 |
| FriOI | GRGCYC | 1 cut(s) | 125 |
| FspBI | CTAG | 1 cut(s) | 291 |
| HaeIII | GGCC | 1 cut(s) | 357 |
| Hin1II | CATG | 5 cut(s) | 23, 230, 349, 387, 397 |
| HinfI | GANTC | 2 cut(s) | 314, 367 |
| HphI | GGTGA | 2 cut(s) | 317, 441 |
| Hpy188I | TCNGA | 1 cut(s) | 49 |
| Hpy188III | TCNNGA | 1 cut(s) | 332 |
| HpyAV | CCTTC | 4 cut(s) | 64, 91, 302, 443 |
| HpyCH4III | ACNGT | 2 cut(s) | 77, 220 |
| HpyCH4IV | ACGT | 2 cut(s) | 44, 177 |
| HpyCH4V | TGCA | 2 cut(s) | 283, 397 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 120, 271, 280, 394 |
| HpySE526I | ACGT | 2 cut(s) | 44, 177 |
| Hsp92II | CATG | 5 cut(s) | 23, 230, 349, 387, 397 |
| LmnI | GCTCC | 1 cut(s) | 349 |
| LpnPI | CCDG | 8 cut(s) | 79, 106, 164, 251, 269, 345, 371, 408 |
| MaeI | CTAG | 1 cut(s) | 291 |
| MaeII | ACGT | 2 cut(s) | 44, 177 |
| MaeIII | GTNAC | 1 cut(s) | 409 |
| MboII | GAAGA | 2 cut(s) | 62, 232 |
| MhlI | GDGCHC | 1 cut(s) | 125 |
| MlsI | TGGCCA | 1 cut(s) | 357 |
| MluCI | AATT | 2 cut(s) | 414, 488 |
| MluNI | TGGCCA | 1 cut(s) | 357 |
| MnlI | CCTC | 7 cut(s) | 46, 101, 122, 214, 248, 317, 384 |
| Mox20I | TGGCCA | 1 cut(s) | 357 |
| MscI | TGGCCA | 1 cut(s) | 357 |
| MseI | TTAA | 1 cut(s) | 339 |
| MslI | CAYNNNNRTG | 1 cut(s) | 231 |
| Msp20I | TGGCCA | 1 cut(s) | 357 |
| MspR9I | CCNGG | 1 cut(s) | 94 |
| MvaI | CCWGG | 1 cut(s) | 94 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 120, 271, 280, 394 |
| NlaIII | CATG | 5 cut(s) | 23, 230, 349, 387, 397 |
| PfeI | GAWTC | 2 cut(s) | 314, 367 |
| PfoI | TCCNGGA | 1 cut(s) | 92 |
| Psp6I | CCWGG | 1 cut(s) | 92 |
| PspGI | CCWGG | 1 cut(s) | 92 |
| PspPI | GGNCC | 1 cut(s) | 148 |
| RsaI | GTAC | 4 cut(s) | 25, 40, 176, 270 |
| RsaNI | GTAC | 4 cut(s) | 24, 39, 175, 269 |
| RseI | CAYNNNNRTG | 1 cut(s) | 231 |
| SaqAI | TTAA | 1 cut(s) | 339 |
| Sau96I | GGNCC | 1 cut(s) | 148 |
| ScrFI | CCNGG | 1 cut(s) | 94 |
| SduI | GDGCHC | 1 cut(s) | 125 |
| SetI | ASST | 9 cut(s) | 47, 102, 116, 153, 180, 276, 307, 354, 460 |
| SinI | GGWCC | 1 cut(s) | 148 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 231 |
| Sse9I | AATT | 2 cut(s) | 414, 488 |
| SspMI | CTAG | 1 cut(s) | 291 |
| StyD4I | CCNGG | 1 cut(s) | 92 |
| TaaI | ACNGT | 2 cut(s) | 77, 220 |
| TaiI | ACGT | 2 cut(s) | 47, 180 |
| TaqI | TCGA | 1 cut(s) | 376 |
| TasI | AATT | 2 cut(s) | 414, 488 |
| TfiI | GAWTC | 2 cut(s) | 314, 367 |
| Tru1I | TTAA | 1 cut(s) | 339 |
| Tru9I | TTAA | 1 cut(s) | 339 |
| TscAI | CASTG | 3 cut(s) | 72, 365, 443 |
| TspDTI | ATGAA | 3 cut(s) | 215, 232, 382 |
| TspGWI | ACGGA | 1 cut(s) | 148 |
| TspRI | CASTG | 3 cut(s) | 72, 365, 443 |
| VpaK11BI | GGWCC | 1 cut(s) | 148 |
| XapI | RAATTY | 1 cut(s) | 488 |
| XspI | CTAG | 1 cut(s) | 291 |