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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 416 |
| AciI | CCGC | 6 cut(s) | 64, 218, 330, 336, 339, 434 |
| AcsI | RAATTY | 1 cut(s) | 344 |
| AfeI | AGCGCT | 1 cut(s) | 141 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 218, 416 |
| AgsI | TTSAA | 2 cut(s) | 136, 431 |
| AluBI | AGCT | 1 cut(s) | 415 |
| AluI | AGCT | 1 cut(s) | 415 |
| Aor51HI | AGCGCT | 1 cut(s) | 141 |
| ApeKI | GCWGC | 2 cut(s) | 197, 333 |
| ApoI | RAATTY | 1 cut(s) | 344 |
| AspLEI | GCGC | 2 cut(s) | 142, 197 |
| AspS9I | GGNCC | 1 cut(s) | 298 |
| AvaII | GGWCC | 1 cut(s) | 298 |
| BanII | GRGCYC | 1 cut(s) | 111 |
| BbvI | GCAGC | 2 cut(s) | 209, 320 |
| BclI | TGATCA | 1 cut(s) | 114 |
| BfaI | CTAG | 1 cut(s) | 38 |
| BfoI | RGCGCY | 1 cut(s) | 143 |
| BisI | GCNGC | 4 cut(s) | 198, 331, 334, 337 |
| BlsI | GCNGC | 4 cut(s) | 199, 332, 335, 338 |
| Bme18I | GGWCC | 1 cut(s) | 298 |
| BmgT120I | GGNCC | 1 cut(s) | 298 |
| BmiI | GGNNCC | 2 cut(s) | 110, 300 |
| BmrI | ACTGGG | 1 cut(s) | 135 |
| BmuI | ACTGGG | 1 cut(s) | 135 |
| BpmI | CTGGAG | 1 cut(s) | 414 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 218, 416 |
| Bse1I | ACTGG | 2 cut(s) | 130, 397 |
| Bse3DI | GCAATG | 1 cut(s) | 213 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 218, 416 |
| BseMI | GCAATG | 1 cut(s) | 213 |
| BseNI | ACTGG | 2 cut(s) | 130, 397 |
| BseRI | GAGGAG | 1 cut(s) | 295 |
| BseXI | GCAGC | 2 cut(s) | 209, 320 |
| BslFI | GGGAC | 3 cut(s) | 143, 284, 403 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 218, 416 |
| BsmFI | GGGAC | 3 cut(s) | 143, 284, 403 |
| BsmI | GAATGC | 1 cut(s) | 156 |
| Bsp1286I | GDGCHC | 1 cut(s) | 111 |
| Bsp143I | GATC | 2 cut(s) | 23, 114 |
| BspACI | CCGC | 6 cut(s) | 64, 218, 330, 336, 339, 434 |
| BspLI | GGNNCC | 2 cut(s) | 110, 300 |
| BsrDI | GCAATG | 1 cut(s) | 213 |
| BsrI | ACTGG | 2 cut(s) | 130, 397 |
| BssMI | GATC | 2 cut(s) | 23, 114 |
| BstC8I | GCNNGC | 1 cut(s) | 144 |
| BstH2I | RGCGCY | 1 cut(s) | 143 |
| BstHHI | GCGC | 2 cut(s) | 142, 197 |
| BstKTI | GATC | 2 cut(s) | 26, 117 |
| BstMBI | GATC | 2 cut(s) | 23, 114 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 336, 431 |
| BstV1I | GCAGC | 2 cut(s) | 209, 320 |
| Cac8I | GCNNGC | 1 cut(s) | 144 |
| CfoI | GCGC | 2 cut(s) | 142, 197 |
| Cfr13I | GGNCC | 1 cut(s) | 298 |
| CviJI | RGCY | 6 cut(s) | 109, 146, 259, 333, 409, 415 |
| CviKI_1 | RGCY | 6 cut(s) | 109, 146, 259, 333, 409, 415 |
| DpnI | GATC | 2 cut(s) | 25, 116 |
| DpnII | GATC | 2 cut(s) | 23, 114 |
| EciI | GGCGGA | 1 cut(s) | 354 |
| Eco24I | GRGCYC | 1 cut(s) | 111 |
| Eco47I | GGWCC | 1 cut(s) | 298 |
| Eco47III | AGCGCT | 1 cut(s) | 141 |
| EcoT38I | GRGCYC | 1 cut(s) | 111 |
| FaiI | YATR | 2 cut(s) | 12, 122 |
| FaqI | GGGAC | 3 cut(s) | 143, 284, 403 |
| FauI | CCCGC | 1 cut(s) | 211 |
| FbaI | TGATCA | 1 cut(s) | 114 |
| Fnu4HI | GCNGC | 4 cut(s) | 198, 331, 334, 337 |
| FriOI | GRGCYC | 1 cut(s) | 111 |
| Fsp4HI | GCNGC | 4 cut(s) | 198, 331, 334, 337 |
| FspBI | CTAG | 1 cut(s) | 38 |
| GlaI | GCGC | 2 cut(s) | 141, 196 |
| GluI | GCNGC | 4 cut(s) | 198, 331, 334, 337 |
| GsuI | CTGGAG | 1 cut(s) | 414 |
| HaeII | RGCGCY | 1 cut(s) | 143 |
| HhaI | GCGC | 2 cut(s) | 142, 197 |
| Hin6I | GCGC | 2 cut(s) | 140, 195 |
| HinP1I | GCGC | 2 cut(s) | 140, 195 |
| HindIII | AAGCTT | 1 cut(s) | 413 |
| HinfI | GANTC | 3 cut(s) | 32, 57, 222 |
| Hpy188I | TCNGA | 3 cut(s) | 28, 54, 266 |
| Hpy188III | TCNNGA | 3 cut(s) | 112, 226, 316 |
| Hpy99I | CGWCG | 1 cut(s) | 170 |
| HpyAV | CCTTC | 1 cut(s) | 320 |
| HpyCH4IV | ACGT | 1 cut(s) | 168 |
| HpyCH4V | TGCA | 1 cut(s) | 380 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 336, 431 |
| HpySE526I | ACGT | 1 cut(s) | 168 |
| HspAI | GCGC | 2 cut(s) | 140, 195 |
| Ksp22I | TGATCA | 1 cut(s) | 114 |
| Kzo9I | GATC | 2 cut(s) | 23, 114 |
| LmnI | GCTCC | 3 cut(s) | 114, 205, 266 |
| LpnPI | CCDG | 8 cut(s) | 80, 111, 125, 128, 132, 188, 239, 378 |
| Lsp1109I | GCAGC | 2 cut(s) | 209, 320 |
| MaeI | CTAG | 1 cut(s) | 38 |
| MaeII | ACGT | 1 cut(s) | 168 |
| MaeIII | GTNAC | 1 cut(s) | 236 |
| MalI | GATC | 2 cut(s) | 25, 116 |
| MboI | GATC | 2 cut(s) | 23, 114 |
| MhlI | GDGCHC | 1 cut(s) | 111 |
| MluCI | AATT | 1 cut(s) | 344 |
| MlyI | GAGTC | 2 cut(s) | 26, 231 |
| MnlI | CCTC | 6 cut(s) | 22, 98, 240, 249, 273, 358 |
| MseI | TTAA | 1 cut(s) | 180 |
| Mva1269I | GAATGC | 1 cut(s) | 156 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 336, 431 |
| NdeII | GATC | 2 cut(s) | 23, 114 |
| NlaIV | GGNNCC | 2 cut(s) | 110, 300 |
| NmuCI | GTSAC | 1 cut(s) | 236 |
| PctI | GAATGC | 1 cut(s) | 156 |
| PfeI | GAWTC | 1 cut(s) | 57 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 416 |
| PkrI | GCNGC | 4 cut(s) | 199, 332, 335, 338 |
| PleI | GAGTC | 2 cut(s) | 26, 230 |
| PpsI | GAGTC | 2 cut(s) | 26, 230 |
| PspN4I | GGNNCC | 2 cut(s) | 110, 300 |
| PspPI | GGNCC | 1 cut(s) | 298 |
| SaqAI | TTAA | 1 cut(s) | 180 |
| SatI | GCNGC | 4 cut(s) | 198, 331, 334, 337 |
| Sau3AI | GATC | 2 cut(s) | 23, 114 |
| Sau96I | GGNCC | 1 cut(s) | 298 |
| SchI | GAGTC | 2 cut(s) | 26, 231 |
| SduI | GDGCHC | 1 cut(s) | 111 |
| SetI | ASST | 3 cut(s) | 171, 186, 417 |
| SinI | GGWCC | 1 cut(s) | 298 |
| Sse9I | AATT | 1 cut(s) | 344 |
| SsiI | CCGC | 6 cut(s) | 64, 218, 330, 336, 339, 434 |
| SspMI | CTAG | 1 cut(s) | 38 |
| TaiI | ACGT | 1 cut(s) | 171 |
| TaqI | TCGA | 1 cut(s) | 22 |
| TasI | AATT | 1 cut(s) | 344 |
| TauI | GCSGC | 2 cut(s) | 333, 339 |
| TfiI | GAWTC | 1 cut(s) | 57 |
| Tru1I | TTAA | 1 cut(s) | 180 |
| Tru9I | TTAA | 1 cut(s) | 180 |
| TseFI | GTSAC | 1 cut(s) | 236 |
| TseI | GCWGC | 2 cut(s) | 197, 333 |
| Tsp45I | GTSAC | 1 cut(s) | 236 |
| TspDTI | ATGAA | 1 cut(s) | 339 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 416 |
| VpaK11BI | GGWCC | 1 cut(s) | 298 |
| XapI | RAATTY | 1 cut(s) | 344 |
| XspI | CTAG | 1 cut(s) | 38 |