Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000373

Physical Location & Seq

Genomic Coordinates Reverse (-)

30062259 .. 30064924

Gene Structure 2666 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1218200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 819 bp

ATGGAGCCCTGCTCAAGTGACGCCGAGGATAAAGGTCTAATTACGCCAGGGAAGACTGTTCTCATTGAGGCCACGGGTGGCAACACCGGCATTGGATTGGCATTCATTGCAGCAAGCAGGGGCTACAAGGTTAAGGTAACCATGCCATCTTCAATGAGTATTGAGAGAAGAATTGTGCTCCTAGCTTTCGGGGCTGAGGTGTACCTCACAGATCCTGCCAAGGGTACTAAGGGAGTCTTTGATAAAGCCGAGGAATTATTGAGTGACACGCCTAATGGTTATATACTTGGCCAATTTGAAAATCCTGCCAATCCGAAGATTCATTATGAAACCACTGGCCCGGAGATATGGAGAGACACAGGAGGGAAGGTTGATGCCCTGGTTTCGGGAATAGGTACTGGGGGTACAGTTGCCGGTGCAGGGACATTCCTTAAAGAGCAGAATCCAGAGATTAAGGTGTACGGTGTAGAACCAGCCGAAAGCCCAGTCTTGAACGGTGGCCAAGCTGGGAAGCATCTGATCCAAGGAATTGGTGCTGGTATCCTACCTGTAGTTCTGGACATTAGCTTGCTTGACGAAGTTATTGAAGTAACAAGTGAGGAAGCAATTGATACCGCGAAGCAGCTTGCCCTGAAAGAAGGTCTTCTGGTGGGGATTTCATCTGGTGCCGCGGCCGCTGCTGCAATAAAGTTGGCTAAGAGGCCGGAAAATGCAGGAAAACTCATTGTTGCTGTGTTCCCCAGTTTTGGAGAGCGTTATCTGTCTTCTGCACTCTTTGATTCCGTTAGGCATGAGGCGGAAAACTTGAGAATTTACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0019344 GO:0019752 GO:0019842 GO:0030170 GO:0036094 GO:0042592 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0048037 GO:0048878 GO:0050662 GO:0050801 GO:0055080 GO:0055081 GO:0065007 GO:0065008 GO:0070279 GO:0071704 GO:0080144 GO:0080145 GO:0080146 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

PALP

Protein Analysis

273

Amino Acids

28.42

Weight (kDa)

4.99

Isoelectric Point (pI)

32.24

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PALP	PF00291	16 - 247	7.7e-60	Pyridoxal-phosphate dependent enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04940 AT3G04940 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28030 AT5G28030 AT5G28030 AT5G28030 AT5G28030
fragaria_vesca	FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830
malus_domestica	MD04G1218200.v1.1 MD04G1218300.v1.1 MD04G1218500.v1.1 MD12G1235500.v1.1
prunus_persica	Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1
pyrus_communis	pycom04g19410 pycom04g19420
rosa_chinensis	RchiOBHm_Chr3g0452261 RchiOBHm_Chr3g0452281 RchiOBHm_Chr3g0452291 RchiOBHm_Chr3g0452301 RchiOBHm_Chr3g0452341 RchiOBHm_Chr3g0452361 RchiOBHm_Chr3g0452371 RchiOBHm_Chr3g0452381 RchiOBHm_Chr3g0452931
rosa_laevigata	RLG00000025606 RLG00000025607 RLG00000025609 RLG00000025610 RLG00000025612 RLG00000025613
rosa_multiflora	Rmu_sc0002823.1_g000002 Rmu_sc0010928.1_g000005 Rmu_sc0010928.1_g000006 Rmu_sc0010928.1_g000011 Rmu_sc0010928.1_g000012 Rmu_sc0010928.1_g000015 Rmu_sc0011680.1_g000012 Rmu_sc0011680.1_g000014 Rmu_sc0011680.1_g000015
rosa_roxburghii	Rroxscaffold_6G00429380 Rroxscaffold_6G00429390 Rroxscaffold_6G00429400 Rroxscaffold_6G00429410 Rroxscaffold_6G00429420
rosa_rugosa	Rorug02G0639700 Rorug02G0639800 Rorug02G0639900 Rorug02G0639900 Rorug02G0639900 Rorug02G0640000 Rorug02G0640000 Rorug02G0640100 Rorug02G0640200 Rorug02G0640300 Rorug02G0640300 Rorug02G0640300 Rorug02G0640300 Rorug02G0640400 Rorug02G0643700
rosa_samantha	Rh3AG041800 Rh3AG042100 Rh3AG042200 Rh3AG046700 Rh3BG043400 Rh3CG041800 Rh3DG042700 Rh3DG042800 Rh3DG043100 Rh3DG043200 Rh3DG048700
rosa_wichuraiana	Rw3G003180 Rw3G003190 Rw3G003200 Rw3G003220 Rw3G003230 Rw3G003240 Rw3G003660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	665
AccII	CGCG	2 cut(s)	617, 671
AciI	CCGC	5 cut(s)	615, 669, 671, 675, 797
AclWI	GGATC	2 cut(s)	206, 514
AcoI	YGGCCR	3 cut(s)	289, 499, 672
AcsI	RAATTY	1 cut(s)	810
AcyI	GRCGYC	1 cut(s)	21
AfaI	GTAC	5 cut(s)	203, 226, 397, 406, 461
AfiI	CCNNNNNNNGG	4 cut(s)	221, 385, 420, 746
AgsI	TTSAA	4 cut(s)	153, 299, 493, 587
AjnI	CCWGG	2 cut(s)	46, 378
AluBI	AGCT	4 cut(s)	185, 506, 567, 625
AluI	AGCT	4 cut(s)	185, 506, 567, 625
Alw21I	GWGCWC	1 cut(s)	180
Alw26I	GTCTC	1 cut(s)	348
AlwI	GGATC	2 cut(s)	206, 514
AlwNI	CAGNNNCTG	1 cut(s)	215
AoxI	GGCC	6 cut(s)	69, 289, 337, 499, 672, 701
ApeKI	GCWGC	4 cut(s)	110, 622, 677, 680
ApoI	RAATTY	1 cut(s)	810
ArsI	GACNNNNNNTTYG	2 cut(s)	471, 503
Asp700I	GAANNNNTTC	1 cut(s)	642
AspS9I	GGNCC	1 cut(s)	338
AsuC2I	CCSGG	1 cut(s)	341
BalI	TGGCCA	2 cut(s)	291, 501
BanI	GGYRCC	1 cut(s)	665
BanII	GRGCYC	1 cut(s)	9
BbsI	GAAGAC	3 cut(s)	59, 635, 756
Bbv12I	GWGCWC	1 cut(s)	180
BbvCI	CCTCAGC	1 cut(s)	195
BbvI	GCAGC	4 cut(s)	122, 634, 664, 667
BccI	CCATC	1 cut(s)	154
BciT130I	CCWGG	2 cut(s)	48, 380
BciVI	GTATCC	1 cut(s)	551
BcnI	CCSGG	1 cut(s)	341
BcoDI	GTCTC	1 cut(s)	348
BfaI	CTAG	1 cut(s)	182
BfmI	CTRYAG	1 cut(s)	549
BfuI	GTATCC	1 cut(s)	551
BisI	GCNGC	7 cut(s)	111, 623, 669, 672, 675, 678, 681
BlsI	GCNGC	7 cut(s)	112, 624, 670, 673, 676, 679, 682
Bme1390I	CCNGG	3 cut(s)	48, 341, 380
BmgT120I	GGNCC	1 cut(s)	338
BmiI	GGNNCC	2 cut(s)	6, 667
BmrFI	CCNGG	3 cut(s)	48, 341, 380
BmrI	ACTGGG	3 cut(s)	408, 479, 735
BmsI	GCATC	2 cut(s)	364, 523
BmuI	ACTGGG	3 cut(s)	408, 479, 735
BpiI	GAAGAC	3 cut(s)	59, 635, 756
BplI	GAGNNNNNCTC	1 cut(s)	28
Bpu10I	CCTNAGC	1 cut(s)	195
BpuMI	CCSGG	1 cut(s)	341
BsaHI	GRCGYC	1 cut(s)	21
BsaJI	CCNNGG	8 cut(s)	24, 47, 72, 219, 249, 378, 523, 669
BsaXI	ACNNNNNCTCC	2 cut(s)	354, 384
Bsc4I	CCNNNNNNNGG	4 cut(s)	221, 385, 420, 746
Bse118I	RCCGGY	2 cut(s)	86, 413
Bse1I	ACTGG	4 cut(s)	340, 403, 485, 741
Bse3DI	GCAATG	1 cut(s)	105
BseBI	CCWGG	2 cut(s)	48, 380
BseDI	CCNNGG	8 cut(s)	24, 47, 72, 219, 249, 378, 523, 669
BseLI	CCNNNNNNNGG	4 cut(s)	221, 385, 420, 746
BseMI	GCAATG	1 cut(s)	105
BseMII	CTCAG	1 cut(s)	186
BseNI	ACTGG	4 cut(s)	340, 403, 485, 741
BseX3I	CGGCCG	1 cut(s)	672
BseXI	GCAGC	4 cut(s)	122, 634, 664, 667
BseYI	CCCAGC	1 cut(s)	506
BsgI	GTGCAG	2 cut(s)	438, 753
Bsh1236I	CGCG	2 cut(s)	617, 671
Bsh1285I	CGRYCG	1 cut(s)	675
BshFI	GGCC	6 cut(s)	71, 291, 339, 501, 674, 703
BshNI	GGYRCC	1 cut(s)	665
BsiEI	CGRYCG	1 cut(s)	675
BsiHKAI	GWGCWC	1 cut(s)	180
BsiSI	CCGG	4 cut(s)	87, 341, 414, 704
BslFI	GGGAC	1 cut(s)	436
BslI	CCNNNNNNNGG	4 cut(s)	221, 385, 420, 746
BsmAI	GTCTC	1 cut(s)	348
BsmFI	GGGAC	1 cut(s)	436
BsmI	GAATGC	1 cut(s)	101
BsnI	GGCC	6 cut(s)	71, 291, 339, 501, 674, 703
Bsp1286I	GDGCHC	2 cut(s)	9, 180
Bsp143I	GATC	2 cut(s)	211, 519
BspACI	CCGC	5 cut(s)	615, 669, 671, 675, 797
BspANI	GGCC	6 cut(s)	71, 291, 339, 501, 674, 703
BspCNI	CTCAG	1 cut(s)	187
BspFNI	CGCG	2 cut(s)	617, 671
BspLI	GGNNCC	2 cut(s)	6, 667
BspPI	GGATC	2 cut(s)	206, 514
BspT107I	GGYRCC	1 cut(s)	665
BsrDI	GCAATG	1 cut(s)	105
BsrFI	RCCGGY	2 cut(s)	86, 413
BsrI	ACTGG	4 cut(s)	340, 403, 485, 741
BssAI	RCCGGY	2 cut(s)	86, 413
BssECI	CCNNGG	8 cut(s)	24, 47, 72, 219, 249, 378, 523, 669
BssMI	GATC	2 cut(s)	211, 519
BssNI	GRCGYC	1 cut(s)	21
BssT1I	CCWWGG	2 cut(s)	219, 523
Bst2UI	CCWGG	2 cut(s)	48, 380
Bst4CI	ACNGT	4 cut(s)	58, 409, 464, 497
BstACI	GRCGYC	1 cut(s)	21
BstAPI	GCANNNNNTGC	1 cut(s)	107
BstC8I	GCNNGC	3 cut(s)	115, 569, 627
BstDEI	CTNAG	3 cut(s)	195, 228, 696
BstDSI	CCRYGG	2 cut(s)	72, 669
BstEII	GGTNACC	1 cut(s)	136
BstFNI	CGCG	2 cut(s)	617, 671
BstKTI	GATC	2 cut(s)	214, 522
BstMAI	GTCTC	1 cut(s)	348
BstMBI	GATC	2 cut(s)	211, 519
BstMCI	CGRYCG	1 cut(s)	675
BstMWI	GCNNNNNNNGC	6 cut(s)	87, 107, 191, 674, 677, 680
BstNI	CCWGG	2 cut(s)	48, 380
BstPI	GGTNACC	1 cut(s)	136
BstSCI	CCNGG	3 cut(s)	46, 339, 378
BstSFI	CTRYAG	1 cut(s)	549
BstUI	CGCG	2 cut(s)	617, 671
BstV1I	GCAGC	4 cut(s)	122, 634, 664, 667
BstV2I	GAAGAC	3 cut(s)	59, 635, 756
BstX2I	RGATCY	1 cut(s)	211
BstXI	CCANNNNNNTGG	1 cut(s)	530
BstYI	RGATCY	1 cut(s)	211
BstZI	CGGCCG	1 cut(s)	672
BsuI	GTATCC	1 cut(s)	551
BsuRI	GGCC	6 cut(s)	71, 291, 339, 501, 674, 703
BtgI	CCRYGG	2 cut(s)	72, 669
BtsIMutI	CAGTG	1 cut(s)	333
Cac8I	GCNNGC	3 cut(s)	115, 569, 627
CaiI	CAGNNNCTG	1 cut(s)	215
CciNI	GCGGCCGC	1 cut(s)	672
Cfr10I	RCCGGY	2 cut(s)	86, 413
Cfr13I	GGNCC	1 cut(s)	338
Cfr42I	CCGCGG	1 cut(s)	672
CseI	GACGC	1 cut(s)	29
Csp6I	GTAC	5 cut(s)	202, 225, 396, 405, 460
CviAII	CATG	2 cut(s)	142, 791
CviQI	GTAC	5 cut(s)	202, 225, 396, 405, 460
DdeI	CTNAG	3 cut(s)	195, 228, 696
DpnI	GATC	2 cut(s)	213, 521
DpnII	GATC	2 cut(s)	211, 519
EaeI	YGGCCR	3 cut(s)	289, 499, 672
EagI	CGGCCG	1 cut(s)	672
EciI	GGCGGA	1 cut(s)	812
EclXI	CGGCCG	1 cut(s)	672
Eco130I	CCWWGG	2 cut(s)	219, 523
Eco24I	GRGCYC	1 cut(s)	9
Eco52I	CGGCCG	1 cut(s)	672
Eco91I	GGTNACC	1 cut(s)	136
EcoO65I	GGTNACC	1 cut(s)	136
EcoRII	CCWGG	2 cut(s)	46, 378
EcoT14I	CCWWGG	2 cut(s)	219, 523
EcoT38I	GRGCYC	1 cut(s)	9
ErhI	CCWWGG	2 cut(s)	219, 523
FaeI	CATG	2 cut(s)	145, 794
FaiI	YATR	6 cut(s)	143, 282, 284, 327, 349, 792
FalI	AAGNNNNNCTT	4 cut(s)	221, 253, 627, 659
FaqI	GGGAC	1 cut(s)	436
FatI	CATG	2 cut(s)	141, 790
Fnu4HI	GCNGC	7 cut(s)	111, 623, 669, 672, 675, 678, 681
FriOI	GRGCYC	1 cut(s)	9
Fsp4HI	GCNGC	7 cut(s)	111, 623, 669, 672, 675, 678, 681
FspBI	CTAG	1 cut(s)	182
GluI	GCNGC	7 cut(s)	111, 623, 669, 672, 675, 678, 681
GsaI	CCCAGC	1 cut(s)	510
HaeIII	GGCC	6 cut(s)	71, 291, 339, 501, 674, 703
HapII	CCGG	4 cut(s)	87, 341, 414, 704
HgaI	GACGC	1 cut(s)	29
Hin1I	GRCGYC	1 cut(s)	21
Hin1II	CATG	2 cut(s)	145, 794
HinfI	GANTC	4 cut(s)	234, 319, 442, 779
HpaII	CCGG	4 cut(s)	87, 341, 414, 704
Hpy166II	GTNNAC	2 cut(s)	202, 460
Hpy188I	TCNGA	2 cut(s)	315, 519
Hpy188III	TCNNGA	4 cut(s)	387, 446, 490, 557
Hpy8I	GTNNAC	2 cut(s)	202, 460
HpyAV	CCTTC	2 cut(s)	361, 632
HpyCH4III	ACNGT	4 cut(s)	58, 409, 464, 497
HpyCH4V	TGCA	5 cut(s)	110, 419, 683, 713, 770
HpyF10VI	GCNNNNNNNGC	6 cut(s)	87, 107, 191, 674, 677, 680
HpyF3I	CTNAG	3 cut(s)	195, 228, 696
Hsp92I	GRCGYC	1 cut(s)	21
Hsp92II	CATG	2 cut(s)	145, 794
KspI	CCGCGG	1 cut(s)	672
Kzo9I	GATC	2 cut(s)	211, 519
LmnI	GCTCC	2 cut(s)	4, 183
Lsp1109I	GCAGC	4 cut(s)	122, 634, 664, 667
LweI	GCATC	2 cut(s)	364, 523
MaeI	CTAG	1 cut(s)	182
MaeIII	GTNAC	4 cut(s)	17, 136, 263, 589
MalI	GATC	2 cut(s)	213, 521
MboI	GATC	2 cut(s)	211, 519
MboII	GAAGA	6 cut(s)	64, 141, 180, 328, 635, 756
MfeI	CAATTG	1 cut(s)	606
MflI	RGATCY	1 cut(s)	211
MhlI	GDGCHC	2 cut(s)	9, 180
MlsI	TGGCCA	2 cut(s)	291, 501
MluCI	AATT	7 cut(s)	39, 171, 254, 293, 528, 606, 810
MluNI	TGGCCA	2 cut(s)	291, 501
MlyI	GAGTC	1 cut(s)	243
MnlI	CCTC	9 cut(s)	19, 61, 190, 215, 244, 356, 592, 693, 787
Mox20I	TGGCCA	2 cut(s)	291, 501
MroXI	GAANNNNTTC	1 cut(s)	642
MscI	TGGCCA	2 cut(s)	291, 501
MseI	TTAA	3 cut(s)	132, 432, 453
Msp20I	TGGCCA	2 cut(s)	291, 501
MspA1I	CMGCKG	2 cut(s)	671, 677
MspI	CCGG	4 cut(s)	87, 341, 414, 704
MspR9I	CCNGG	3 cut(s)	48, 341, 380
MunI	CAATTG	1 cut(s)	606
Mva1269I	GAATGC	1 cut(s)	101
MvaI	CCWGG	2 cut(s)	48, 380
MvnI	CGCG	2 cut(s)	617, 671
MwoI	GCNNNNNNNGC	6 cut(s)	87, 107, 191, 674, 677, 680
NciI	CCSGG	1 cut(s)	341
NdeII	GATC	2 cut(s)	211, 519
NlaIII	CATG	2 cut(s)	145, 794
NlaIV	GGNNCC	2 cut(s)	6, 667
NmeAIII	GCCGAG	2 cut(s)	49, 274
NmuCI	GTSAC	2 cut(s)	17, 263
NotI	GCGGCCGC	1 cut(s)	672
PctI	GAATGC	1 cut(s)	101
PdmI	GAANNNNTTC	1 cut(s)	642
PfeI	GAWTC	3 cut(s)	319, 442, 779
PkrI	GCNGC	7 cut(s)	112, 624, 670, 673, 676, 679, 682
PleI	GAGTC	1 cut(s)	242
PpsI	GAGTC	1 cut(s)	242
Psp6I	CCWGG	2 cut(s)	46, 378
PspEI	GGTNACC	1 cut(s)	136
PspFI	CCCAGC	1 cut(s)	506
PspGI	CCWGG	2 cut(s)	46, 378
PspN4I	GGNNCC	2 cut(s)	6, 667
PspPI	GGNCC	1 cut(s)	338
PstNI	CAGNNNCTG	1 cut(s)	215
PsuI	RGATCY	1 cut(s)	211
RsaI	GTAC	5 cut(s)	203, 226, 397, 406, 461
RsaNI	GTAC	5 cut(s)	202, 225, 396, 405, 460
SacII	CCGCGG	1 cut(s)	672
SaqAI	TTAA	3 cut(s)	132, 432, 453
SatI	GCNGC	7 cut(s)	111, 623, 669, 672, 675, 678, 681
Sau3AI	GATC	2 cut(s)	211, 519
Sau96I	GGNCC	1 cut(s)	338
SchI	GAGTC	1 cut(s)	243
ScrFI	CCNGG	3 cut(s)	48, 341, 380
SduI	GDGCHC	2 cut(s)	9, 180
SfaNI	GCATC	2 cut(s)	364, 523
SfcI	CTRYAG	1 cut(s)	549
Sfr303I	CCGCGG	1 cut(s)	672
SgrBI	CCGCGG	1 cut(s)	672
SmlI	CTYRAG	2 cut(s)	13, 805
SmoI	CTYRAG	2 cut(s)	13, 805
Sse9I	AATT	7 cut(s)	39, 171, 254, 293, 528, 606, 810
SsiI	CCGC	5 cut(s)	615, 669, 671, 675, 797
SspMI	CTAG	1 cut(s)	182
StyD4I	CCNGG	3 cut(s)	46, 339, 378
StyI	CCWWGG	2 cut(s)	219, 523
TaaI	ACNGT	4 cut(s)	58, 409, 464, 497
TasI	AATT	7 cut(s)	39, 171, 254, 293, 528, 606, 810
TauI	GCSGC	3 cut(s)	671, 674, 677
TfiI	GAWTC	3 cut(s)	319, 442, 779
Tru1I	TTAA	3 cut(s)	132, 432, 453
Tru9I	TTAA	3 cut(s)	132, 432, 453
TscAI	CASTG	1 cut(s)	340
TseFI	GTSAC	2 cut(s)	17, 263
TseI	GCWGC	4 cut(s)	110, 622, 677, 680
Tsp45I	GTSAC	2 cut(s)	17, 263
TspDTI	ATGAA	4 cut(s)	94, 311, 342, 648
TspGWI	ACGGA	1 cut(s)	772
TspRI	CASTG	1 cut(s)	340
XapI	RAATTY	1 cut(s)	810
XmnI	GAANNNNTTC	1 cut(s)	642
XspI	CTAG	1 cut(s)	182

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.