Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0000373

Physical Location & Seq

Genomic Coordinates Reverse (-)

31008194 .. 31010521

Gene Structure 2328 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1235500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGAGGGCAACTGTGCAATCAGGAAAGATGTCACCGAATTAATCGGCAATACCCCGATGGTGTATCTCAATAATGTTGTGGATGGTTGTGTAGCTCGAATTGCTGCCAAGCTAGAGACAATGGAACCCTGCTCTAGTGTGAAAGACAGAATTGCGTATAGCATGATCAAGGACGCAGAGGATAAAGGCCTGATTACGCCAGGGGAGACTGTCCTCGTTGAGCCCACGAGTGGAAACACCGGCATTGGATTAGCGTTCCTTGCAGCTAGCAAGGGTTACAAGCTCAAGCTAAGCATGCCATCCATAGTGAGTCTCGAAAGAAGAATCGTGCTCCGAGCTTTTGGAGCTGAGGTGTACATAACAGATCCTGCCAAGGGTATTAAGGGAGTTTTTGATAAGGCCGGGGAGTTGCTGAGTAACACACCTAATAGTCATATGCTTTGCCAATTTGAAAATCCTGCAAATCCAAAGATTCACTATGAAACCACTGGCCCGGAGATATGGAGAGACTCGGGAGGGAAAGTTGATGCTCTAGTTGCAGGGATAGGGACTGGAGGGACAGTAACTGGTGCAGGGAAGTTCCTAAAAGAGAAAAATTCCGACATCAAGGTTTACGGTGTAGAGCCAGCCGAAAGTGCTGTGTTGAATGGTGGCACACCAGGGATGCATCTGATCCAAGGAATTGGTGCCGGTTTCATACCTAAAGTTCTGGATGTTAGCCTTCTCGACGAAGTTATTCAAGTATCCAGCGAGGAAGCTATCAAAACTTCTAAGCTACTTGCCCTAAAAGAAGGTCTTCTGGTGGGGATTTCATCTGGTGCTGCAGCAGCTGCTGGTATAAAGTTGGCAAAGAGGCAAGAAAATGCTGGGAAACTCATTGTTGTTGTGTTCCCCAGCTTCGGAGAGCGTTATCTGTCTACTGCGCTCTTCGATTCCATTAGGCTTGACGCAGAAAACATGAAATTCGACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000096 GO:0000097 GO:0003674 GO:0003824 GO:0004124 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006082 GO:0006520 GO:0006534 GO:0006535 GO:0006563 GO:0006790 GO:0006807 GO:0008144 GO:0008150 GO:0008152 GO:0008652 GO:0009058 GO:0009069 GO:0009070 GO:0009987 GO:0016053 GO:0016740 GO:0016765 GO:0016829 GO:0016846 GO:0019344 GO:0019752 GO:0019842 GO:0030170 GO:0036094 GO:0042592 GO:0043167 GO:0043168 GO:0043436 GO:0044237 GO:0044238 GO:0044249 GO:0044272 GO:0044281 GO:0044283 GO:0044424 GO:0044464 GO:0046394 GO:0048037 GO:0048878 GO:0050662 GO:0050801 GO:0055080 GO:0055081 GO:0065007 GO:0065008 GO:0070279 GO:0071704 GO:0080144 GO:0080145 GO:0080146 GO:0097159 GO:1901363 GO:1901564 GO:1901566 GO:1901576 GO:1901605 GO:1901607

KEGG Pathways

Metabolic & Signaling

ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230

Pfam Domains

Protein Families

PALP

Protein Analysis

324

Amino Acids

34.15

Weight (kDa)

5.81

Isoelectric Point (pI)

14.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PALP	PF00291	11 - 298	3e-70	Pyridoxal-phosphate dependent enzyme

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0000373)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G04940 AT3G04940 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28020 AT5G28030 AT5G28030 AT5G28030 AT5G28030 AT5G28030
fragaria_vesca	FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03790 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830 FvH4_6g03830
malus_domestica	MD04G1218200.v1.1 MD04G1218300.v1.1 MD04G1218500.v1.1 MD12G1235500.v1.1
prunus_persica	Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1 Prupe.6G338100_v2.0.a1
pyrus_communis	pycom04g19410 pycom04g19420
rosa_chinensis	RchiOBHm_Chr3g0452261 RchiOBHm_Chr3g0452281 RchiOBHm_Chr3g0452291 RchiOBHm_Chr3g0452301 RchiOBHm_Chr3g0452341 RchiOBHm_Chr3g0452361 RchiOBHm_Chr3g0452371 RchiOBHm_Chr3g0452381 RchiOBHm_Chr3g0452931
rosa_laevigata	RLG00000025606 RLG00000025607 RLG00000025609 RLG00000025610 RLG00000025612 RLG00000025613
rosa_multiflora	Rmu_sc0002823.1_g000002 Rmu_sc0010928.1_g000005 Rmu_sc0010928.1_g000006 Rmu_sc0010928.1_g000011 Rmu_sc0010928.1_g000012 Rmu_sc0010928.1_g000015 Rmu_sc0011680.1_g000012 Rmu_sc0011680.1_g000014 Rmu_sc0011680.1_g000015
rosa_roxburghii	Rroxscaffold_6G00429380 Rroxscaffold_6G00429390 Rroxscaffold_6G00429400 Rroxscaffold_6G00429410 Rroxscaffold_6G00429420
rosa_rugosa	Rorug02G0639700 Rorug02G0639800 Rorug02G0639900 Rorug02G0639900 Rorug02G0639900 Rorug02G0640000 Rorug02G0640000 Rorug02G0640100 Rorug02G0640200 Rorug02G0640300 Rorug02G0640300 Rorug02G0640300 Rorug02G0640300 Rorug02G0640400 Rorug02G0643700
rosa_samantha	Rh3AG041800 Rh3AG042100 Rh3AG042200 Rh3AG046700 Rh3BG043400 Rh3CG041800 Rh3DG042700 Rh3DG042800 Rh3DG043100 Rh3DG043200 Rh3DG048700
rosa_wichuraiana	Rw3G003180 Rw3G003190 Rw3G003200 Rw3G003220 Rw3G003230 Rw3G003240 Rw3G003660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	686
AccI	GTMKAC	1 cut(s)	917
AclWI	GGATC	2 cut(s)	359, 667
AcsI	RAATTY	2 cut(s)	595, 962
AfaI	GTAC	1 cut(s)	356
AfiI	CCNNNNNNNGG	3 cut(s)	230, 374, 899
AgsI	TTSAA	3 cut(s)	452, 646, 740
AjnI	CCWGG	2 cut(s)	199, 658
Alw21I	GWGCWC	1 cut(s)	333
Alw26I	GTCTC	4 cut(s)	110, 200, 317, 501
AlwI	GGATC	2 cut(s)	359, 667
AlwNI	CAGNNNCTG	4 cut(s)	368, 566, 830, 833
Ama87I	CYCGRG	1 cut(s)	511
AoxI	GGCC	3 cut(s)	187, 399, 490
ApeKI	GCWGC	6 cut(s)	104, 263, 821, 824, 827, 830
ApoI	RAATTY	2 cut(s)	595, 962
AseI	ATTAAT	1 cut(s)	41
Asp700I	GAANNNNTTC	2 cut(s)	735, 795
AspLEI	GCGC	1 cut(s)	925
AspS9I	GGNCC	1 cut(s)	491
AsuC2I	CCSGG	2 cut(s)	403, 494
AsuHPI	GGTGA	1 cut(s)	25
AsuNHI	GCTAGC	1 cut(s)	266
AvaI	CYCGRG	1 cut(s)	511
BanI	GGYRCC	1 cut(s)	686
BanII	GRGCYC	1 cut(s)	225
BauI	CACGAG	1 cut(s)	226
BbsI	GAAGAC	1 cut(s)	788
Bbv12I	GWGCWC	1 cut(s)	333
BbvCI	CCTCAGC	1 cut(s)	348
BbvI	GCAGC	6 cut(s)	91, 275, 808, 817, 836, 839
BccI	CCATC	3 cut(s)	52, 77, 307
BciT130I	CCWGG	2 cut(s)	201, 660
BciVI	GTATCC	1 cut(s)	754
BclI	TGATCA	1 cut(s)	165
BcnI	CCSGG	2 cut(s)	403, 494
BcoDI	GTCTC	4 cut(s)	110, 200, 317, 501
BfaI	CTAG	4 cut(s)	113, 135, 267, 533
BfmI	CTRYAG	1 cut(s)	822
BfuI	GTATCC	1 cut(s)	754
BisI	GCNGC	6 cut(s)	105, 264, 822, 825, 828, 831
BlpI	GCTNAGC	1 cut(s)	290
BlsI	GCNGC	6 cut(s)	106, 265, 823, 826, 829, 832
Bme1390I	CCNGG	4 cut(s)	201, 403, 494, 660
BmeT110I	CYCGRG	1 cut(s)	511
BmgT120I	GGNCC	1 cut(s)	491
BmiI	GGNNCC	2 cut(s)	126, 688
BmrFI	CCNGG	4 cut(s)	201, 403, 494, 660
BmsI	GCATC	3 cut(s)	517, 654, 676
BmtI	GCTAGC	1 cut(s)	270
BpiI	GAAGAC	1 cut(s)	788
BpmI	CTGGAG	1 cut(s)	573
Bpu10I	CCTNAGC	1 cut(s)	348
Bpu1102I	GCTNAGC	1 cut(s)	290
BpuEI	CTTGAG	1 cut(s)	269
BpuMI	CCSGG	2 cut(s)	403, 494
BsaJI	CCNNGG	5 cut(s)	200, 372, 402, 659, 676
BsaXI	ACNNNNNCTCC	6 cut(s)	336, 366, 507, 537, 546, 576
Bsc4I	CCNNNNNNNGG	3 cut(s)	230, 374, 899
Bse118I	RCCGGY	2 cut(s)	239, 689
Bse1I	ACTGG	3 cut(s)	493, 556, 571
BseBI	CCWGG	2 cut(s)	201, 660
BseDI	CCNNGG	5 cut(s)	200, 372, 402, 659, 676
BseGI	GGATG	4 cut(s)	88, 299, 669, 718
BseLI	CCNNNNNNNGG	3 cut(s)	230, 374, 899
BseMII	CTCAG	2 cut(s)	339, 404
BseNI	ACTGG	3 cut(s)	493, 556, 571
BseXI	GCAGC	6 cut(s)	91, 275, 808, 817, 836, 839
BseYI	CCCAGC	2 cut(s)	866, 893
BsgI	GTGCAG	1 cut(s)	591
BshFI	GGCC	3 cut(s)	189, 401, 492
BshNI	GGYRCC	1 cut(s)	686
BsiHKAI	GWGCWC	1 cut(s)	333
BsiHKCI	CYCGRG	1 cut(s)	511
BsiSI	CCGG	4 cut(s)	240, 402, 494, 690
BslFI	GGGAC	2 cut(s)	562, 571
BslI	CCNNNNNNNGG	3 cut(s)	230, 374, 899
BsmAI	GTCTC	4 cut(s)	110, 200, 317, 501
BsmFI	GGGAC	2 cut(s)	562, 571
BsnI	GGCC	3 cut(s)	189, 401, 492
BsoBI	CYCGRG	1 cut(s)	511
Bsp1286I	GDGCHC	2 cut(s)	225, 333
Bsp1407I	TGTACA	1 cut(s)	354
Bsp143I	GATC	3 cut(s)	165, 364, 672
Bsp1720I	GCTNAGC	1 cut(s)	290
BspANI	GGCC	3 cut(s)	189, 401, 492
BspCNI	CTCAG	2 cut(s)	340, 405
BspLI	GGNNCC	2 cut(s)	126, 688
BspMAI	CTGCAG	1 cut(s)	826
BspOI	GCTAGC	1 cut(s)	270
BspPI	GGATC	2 cut(s)	359, 667
BspQI	GCTCTTC	1 cut(s)	932
BspT107I	GGYRCC	1 cut(s)	686
BsrFI	RCCGGY	2 cut(s)	239, 689
BsrGI	TGTACA	1 cut(s)	354
BsrI	ACTGG	3 cut(s)	493, 556, 571
BssAI	RCCGGY	2 cut(s)	239, 689
BssECI	CCNNGG	5 cut(s)	200, 372, 402, 659, 676
BssMI	GATC	3 cut(s)	165, 364, 672
BssSI	CACGAG	1 cut(s)	226
BssT1I	CCWWGG	2 cut(s)	372, 676
Bst2BI	CACGAG	1 cut(s)	226
Bst2UI	CCWGG	2 cut(s)	201, 660
Bst4CI	ACNGT	4 cut(s)	14, 211, 562, 617
Bst6I	CTCTTC	1 cut(s)	932
BstAPI	GCANNNNNTGC	1 cut(s)	830
BstAUI	TGTACA	1 cut(s)	354
BstC8I	GCNNGC	3 cut(s)	268, 296, 627
BstDEI	CTNAG	4 cut(s)	290, 348, 413, 771
BstF5I	GGATG	4 cut(s)	88, 299, 669, 718
BstHHI	GCGC	1 cut(s)	925
BstKTI	GATC	3 cut(s)	168, 367, 675
BstMAI	GTCTC	4 cut(s)	110, 200, 317, 501
BstMBI	GATC	3 cut(s)	165, 364, 672
BstMWI	GCNNNNNNNGC	8 cut(s)	101, 260, 295, 344, 536, 635, 827, 830
BstNI	CCWGG	2 cut(s)	201, 660
BstNSI	RCATGY	1 cut(s)	298
BstSCI	CCNGG	4 cut(s)	199, 401, 492, 658
BstSFI	CTRYAG	1 cut(s)	822
BstV1I	GCAGC	6 cut(s)	91, 275, 808, 817, 836, 839
BstV2I	GAAGAC	1 cut(s)	788
BstX2I	RGATCY	1 cut(s)	364
BstXI	CCANNNNNNTGG	1 cut(s)	683
BstYI	RGATCY	1 cut(s)	364
BsuI	GTATCC	1 cut(s)	754
BsuRI	GGCC	3 cut(s)	189, 401, 492
BtsCI	GGATG	4 cut(s)	88, 299, 669, 718
BtsIMutI	CAGTG	1 cut(s)	486
Cac8I	GCNNGC	3 cut(s)	268, 296, 627
CaiI	CAGNNNCTG	4 cut(s)	368, 566, 830, 833
CfoI	GCGC	1 cut(s)	925
Cfr10I	RCCGGY	2 cut(s)	239, 689
Cfr13I	GGNCC	1 cut(s)	491
CseI	GACGC	2 cut(s)	182, 956
Csp6I	GTAC	1 cut(s)	355
CviAII	CATG	3 cut(s)	163, 295, 958
CviQI	GTAC	1 cut(s)	355
DdeI	CTNAG	4 cut(s)	290, 348, 413, 771
DpnI	GATC	3 cut(s)	167, 366, 674
DpnII	GATC	3 cut(s)	165, 364, 672
Eam1104I	CTCTTC	1 cut(s)	932
EarI	CTCTTC	1 cut(s)	932
Eco130I	CCWWGG	2 cut(s)	372, 676
Eco147I	AGGCCT	1 cut(s)	189
Eco24I	GRGCYC	1 cut(s)	225
Eco88I	CYCGRG	1 cut(s)	511
EcoRII	CCWGG	2 cut(s)	199, 658
EcoT14I	CCWWGG	2 cut(s)	372, 676
EcoT22I	ATGCAT	1 cut(s)	669
EcoT38I	GRGCYC	1 cut(s)	225
ErhI	CCWWGG	2 cut(s)	372, 676
FaeI	CATG	3 cut(s)	166, 298, 961
FalI	AAGNNNNNCTT	2 cut(s)	780, 812
FaqI	GGGAC	2 cut(s)	562, 571
FatI	CATG	3 cut(s)	162, 294, 957
FauNDI	CATATG	1 cut(s)	435
FbaI	TGATCA	1 cut(s)	165
FblI	GTMKAC	1 cut(s)	917
Fnu4HI	GCNGC	6 cut(s)	105, 264, 822, 825, 828, 831
FokI	GGATG	4 cut(s)	95, 286, 676, 725
FriOI	GRGCYC	1 cut(s)	225
Fsp4HI	GCNGC	6 cut(s)	105, 264, 822, 825, 828, 831
FspBI	CTAG	4 cut(s)	113, 135, 267, 533
GlaI	GCGC	1 cut(s)	924
GluI	GCNGC	6 cut(s)	105, 264, 822, 825, 828, 831
GsaI	CCCAGC	2 cut(s)	870, 897
GsuI	CTGGAG	1 cut(s)	573
HaeIII	GGCC	3 cut(s)	189, 401, 492
HapII	CCGG	4 cut(s)	240, 402, 494, 690
HgaI	GACGC	2 cut(s)	182, 956
HhaI	GCGC	1 cut(s)	925
Hin1II	CATG	3 cut(s)	166, 298, 961
Hin6I	GCGC	1 cut(s)	923
HinP1I	GCGC	1 cut(s)	923
HinfI	GANTC	5 cut(s)	310, 324, 472, 509, 932
HpaII	CCGG	4 cut(s)	240, 402, 494, 690
HphI	GGTGA	1 cut(s)	25
Hpy166II	GTNNAC	3 cut(s)	355, 613, 918
Hpy188I	TCNGA	4 cut(s)	335, 601, 672, 902
Hpy188III	TCNNGA	5 cut(s)	22, 314, 513, 710, 725
Hpy8I	GTNNAC	3 cut(s)	355, 613, 918
Hpy99I	CGWCG	1 cut(s)	731
HpyAV	CCTTC	2 cut(s)	731, 785
HpyCH4III	ACNGT	4 cut(s)	14, 211, 562, 617
HpyCH4V	TGCA	7 cut(s)	17, 263, 461, 539, 572, 667, 824
HpyF10VI	GCNNNNNNNGC	8 cut(s)	101, 260, 295, 344, 536, 635, 827, 830
HpyF3I	CTNAG	4 cut(s)	290, 348, 413, 771
Hsp92II	CATG	3 cut(s)	166, 298, 961
HspAI	GCGC	1 cut(s)	923
Ksp22I	TGATCA	1 cut(s)	165
Kzo9I	GATC	3 cut(s)	165, 364, 672
LguI	GCTCTTC	1 cut(s)	932
LmnI	GCTCC	2 cut(s)	336, 344
Lsp1109I	GCAGC	6 cut(s)	91, 275, 808, 817, 836, 839
LweI	GCATC	3 cut(s)	517, 654, 676
MaeI	CTAG	4 cut(s)	113, 135, 267, 533
MaeIII	GTNAC	4 cut(s)	31, 275, 416, 562
MalI	GATC	3 cut(s)	167, 366, 674
MboI	GATC	3 cut(s)	165, 364, 672
MboII	GAAGA	3 cut(s)	333, 788, 919
MflI	RGATCY	1 cut(s)	364
MhlI	GDGCHC	2 cut(s)	225, 333
MluCI	AATT	7 cut(s)	38, 99, 150, 446, 595, 681, 962
MlyI	GAGTC	2 cut(s)	319, 503
MmeI	TCCRAC	1 cut(s)	624
MnlI	CCTC	7 cut(s)	172, 224, 343, 509, 548, 745, 846
Mph1103I	ATGCAT	1 cut(s)	669
MroXI	GAANNNNTTC	2 cut(s)	735, 795
MseI	TTAA	2 cut(s)	41, 381
MspA1I	CMGCKG	1 cut(s)	830
MspI	CCGG	4 cut(s)	240, 402, 494, 690
MspR9I	CCNGG	4 cut(s)	201, 403, 494, 660
MvaI	CCWGG	2 cut(s)	201, 660
MwoI	GCNNNNNNNGC	8 cut(s)	101, 260, 295, 344, 536, 635, 827, 830
NciI	CCSGG	2 cut(s)	403, 494
NdeI	CATATG	1 cut(s)	435
NdeII	GATC	3 cut(s)	165, 364, 672
NheI	GCTAGC	1 cut(s)	266
NlaIII	CATG	3 cut(s)	166, 298, 961
NlaIV	GGNNCC	2 cut(s)	126, 688
NmuCI	GTSAC	1 cut(s)	31
NsiI	ATGCAT	1 cut(s)	669
NspI	RCATGY	1 cut(s)	298
PaeI	GCATGC	1 cut(s)	298
PceI	AGGCCT	1 cut(s)	189
PciSI	GCTCTTC	1 cut(s)	932
PdmI	GAANNNNTTC	2 cut(s)	735, 795
PfeI	GAWTC	3 cut(s)	324, 472, 932
PkrI	GCNGC	6 cut(s)	106, 265, 823, 826, 829, 832
PleI	GAGTC	2 cut(s)	318, 503
PpsI	GAGTC	2 cut(s)	318, 503
PshBI	ATTAAT	1 cut(s)	41
Psp6I	CCWGG	2 cut(s)	199, 658
PspFI	CCCAGC	2 cut(s)	866, 893
PspGI	CCWGG	2 cut(s)	199, 658
PspN4I	GGNNCC	2 cut(s)	126, 688
PspPI	GGNCC	1 cut(s)	491
PstI	CTGCAG	1 cut(s)	826
PstNI	CAGNNNCTG	4 cut(s)	368, 566, 830, 833
PsuI	RGATCY	1 cut(s)	364
PvuII	CAGCTG	1 cut(s)	830
RsaI	GTAC	1 cut(s)	356
RsaNI	GTAC	1 cut(s)	355
SapI	GCTCTTC	1 cut(s)	932
SaqAI	TTAA	2 cut(s)	41, 381
SatI	GCNGC	6 cut(s)	105, 264, 822, 825, 828, 831
Sau3AI	GATC	3 cut(s)	165, 364, 672
Sau96I	GGNCC	1 cut(s)	491
SchI	GAGTC	2 cut(s)	319, 503
ScrFI	CCNGG	4 cut(s)	201, 403, 494, 660
SduI	GDGCHC	2 cut(s)	225, 333
SfaNI	GCATC	3 cut(s)	517, 654, 676
SfcI	CTRYAG	1 cut(s)	822
SmlI	CTYRAG	1 cut(s)	284
SmoI	CTYRAG	1 cut(s)	284
SphI	GCATGC	1 cut(s)	298
Sse9I	AATT	7 cut(s)	38, 99, 150, 446, 595, 681, 962
SseBI	AGGCCT	1 cut(s)	189
SspMI	CTAG	4 cut(s)	113, 135, 267, 533
StuI	AGGCCT	1 cut(s)	189
StyD4I	CCNGG	4 cut(s)	199, 401, 492, 658
StyI	CCWWGG	2 cut(s)	372, 676
TaaI	ACNGT	4 cut(s)	14, 211, 562, 617
TaqI	TCGA	5 cut(s)	97, 315, 726, 930, 966
TasI	AATT	7 cut(s)	38, 99, 150, 446, 595, 681, 962
TatI	WGTACW	1 cut(s)	354
TfiI	GAWTC	3 cut(s)	324, 472, 932
Tru1I	TTAA	2 cut(s)	41, 381
Tru9I	TTAA	2 cut(s)	41, 381
TscAI	CASTG	1 cut(s)	493
TseFI	GTSAC	1 cut(s)	31
TseI	GCWGC	6 cut(s)	104, 263, 821, 824, 827, 830
Tsp45I	GTSAC	1 cut(s)	31
TspDTI	ATGAA	3 cut(s)	495, 685, 801
TspRI	CASTG	1 cut(s)	493
VspI	ATTAAT	1 cut(s)	41
XapI	RAATTY	2 cut(s)	595, 962
XceI	RCATGY	1 cut(s)	298
XmiI	GTMKAC	1 cut(s)	917
XmnI	GAANNNNTTC	2 cut(s)	735, 795
XspI	CTAG	4 cut(s)	113, 135, 267, 533
Zsp2I	ATGCAT	1 cut(s)	669

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.