Belongs to the cysteine synthase cystathionine beta- synthase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| Ank_2 | PF12796 | 84 - 163 | 1.2e-06 | Ankyrin repeats (3 copies) |
| Ank_2 | PF12796 | 343 - 401 | 1.4e-08 | Ankyrin repeats (3 copies) |
| Ank_2 | PF12796 | 448 - 528 | 1.8e-08 | Ankyrin repeats (3 copies) |
| Ank_2 | PF12796 | 549 - 621 | 2.5e-12 | Ankyrin repeats (3 copies) |
| Ank_4 | PF13637 | 549 - 598 | 8.8e-09 | Ankyrin repeats (many copies) |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 2 cut(s) | 135, 243 |
| Acc36I | ACCTGC | 1 cut(s) | 676 |
| Acc65I | GGTACC | 1 cut(s) | 1772 |
| AccB1I | GGYRCC | 3 cut(s) | 146, 980, 1772 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 825 |
| AccI | GTMKAC | 2 cut(s) | 129, 648 |
| AccII | CGCG | 2 cut(s) | 344, 789 |
| AciI | CCGC | 6 cut(s) | 114, 187, 499, 909, 1048, 1959 |
| AclWI | GGATC | 3 cut(s) | 113, 382, 490 |
| AcoI | YGGCCR | 3 cut(s) | 198, 1179, 2193 |
| AcsI | RAATTY | 2 cut(s) | 1403, 1598 |
| AcuI | CTGAAG | 2 cut(s) | 113, 599 |
| AcyI | GRCGYC | 3 cut(s) | 132, 147, 240 |
| AdeI | CACNNNGTG | 1 cut(s) | 1664 |
| AfaI | GTAC | 2 cut(s) | 516, 1774 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 825, 908, 1489, 2202 |
| AjiI | CACGTC | 1 cut(s) | 1661 |
| AjnI | CCWGG | 1 cut(s) | 1757 |
| Alw21I | GWGCWC | 1 cut(s) | 661 |
| Alw26I | GTCTC | 3 cut(s) | 1118, 1172, 1529 |
| Alw44I | GTGCAC | 1 cut(s) | 657 |
| AlwI | GGATC | 3 cut(s) | 113, 382, 490 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1118, 1505 |
| Ama87I | CYCGRG | 2 cut(s) | 383, 1935 |
| AoxI | GGCC | 7 cut(s) | 45, 198, 367, 1179, 1374, 1803, 2193 |
| ApaLI | GTGCAC | 1 cut(s) | 657 |
| ApoI | RAATTY | 2 cut(s) | 1403, 1598 |
| ArsI | GACNNNNNNTTYG | 8 cut(s) | 122, 154, 198, 225, 230, 257, 1241, 1273 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 329 |
| Asp718I | GGTACC | 1 cut(s) | 1772 |
| AspLEI | GCGC | 5 cut(s) | 63, 113, 149, 789, 967 |
| AspS9I | GGNCC | 3 cut(s) | 367, 1257, 1803 |
| AsuC2I | CCSGG | 2 cut(s) | 32, 2078 |
| AsuHPI | GGTGA | 7 cut(s) | 298, 1133, 1376, 1544, 1600, 2077, 2218 |
| AsuNHI | GCTAGC | 1 cut(s) | 2072 |
| AvaI | CYCGRG | 2 cut(s) | 383, 1935 |
| AvaII | GGWCC | 1 cut(s) | 1257 |
| BaeGI | GKGCMC | 1 cut(s) | 661 |
| BalI | TGGCCA | 1 cut(s) | 1181 |
| BanI | GGYRCC | 3 cut(s) | 146, 980, 1772 |
| BanII | GRGCYC | 1 cut(s) | 1936 |
| BbsI | GAAGAC | 1 cut(s) | 1022 |
| Bbv12I | GWGCWC | 1 cut(s) | 661 |
| BbvCI | CCTCAGC | 1 cut(s) | 2061 |
| BccI | CCATC | 7 cut(s) | 97, 378, 513, 941, 1718, 1724, 2013 |
| BceAI | ACGGC | 3 cut(s) | 134, 245, 261 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 575, 609, 638, 672 |
| BciT130I | CCWGG | 1 cut(s) | 1759 |
| BciVI | GTATCC | 1 cut(s) | 600 |
| BclI | TGATCA | 1 cut(s) | 1449 |
| BcnI | CCSGG | 2 cut(s) | 32, 2078 |
| BcoDI | GTCTC | 3 cut(s) | 1118, 1172, 1529 |
| BfaI | CTAG | 3 cut(s) | 852, 1811, 2073 |
| BfmI | CTRYAG | 5 cut(s) | 538, 652, 669, 771, 1386 |
| BfoI | RGCGCY | 2 cut(s) | 64, 150 |
| BfuAI | ACCTGC | 1 cut(s) | 676 |
| BfuI | GTATCC | 1 cut(s) | 600 |
| BglII | AGATCT | 2 cut(s) | 88, 1984 |
| BlpI | GCTNAGC | 2 cut(s) | 299, 1884 |
| Bme1390I | CCNGG | 3 cut(s) | 32, 1759, 2078 |
| Bme18I | GGWCC | 1 cut(s) | 1257 |
| BmeT110I | CYCGRG | 2 cut(s) | 383, 1935 |
| BmgBI | CACGTC | 1 cut(s) | 1661 |
| BmgT120I | GGNCC | 3 cut(s) | 367, 1257, 1803 |
| BmiI | GGNNCC | 5 cut(s) | 148, 982, 1259, 1774, 1804 |
| BmrFI | CCNGG | 3 cut(s) | 32, 1759, 2078 |
| BmrI | ACTGGG | 1 cut(s) | 1020 |
| BmsI | GCATC | 5 cut(s) | 416, 577, 1581, 1666, 1870 |
| BmtI | GCTAGC | 1 cut(s) | 2076 |
| BmuI | ACTGGG | 1 cut(s) | 1020 |
| BoxI | GACNNNNGTC | 1 cut(s) | 731 |
| BpiI | GAAGAC | 1 cut(s) | 1022 |
| BpmI | CTGGAG | 2 cut(s) | 423, 2145 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2061 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 299, 1884 |
| BpuEI | CTTGAG | 2 cut(s) | 1341, 1928 |
| BpuMI | CCSGG | 2 cut(s) | 32, 2078 |
| BsaAI | YACGTR | 1 cut(s) | 1928 |
| BsaBI | GATNNNNATC | 1 cut(s) | 318 |
| BsaHI | GRCGYC | 3 cut(s) | 132, 147, 240 |
| BsaJI | CCNNGG | 6 cut(s) | 48, 334, 1051, 1182, 1261, 1758 |
| BsaWI | WCCGGW | 1 cut(s) | 1482 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 300, 330, 1494, 1524, 2092, 2122 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 825, 908, 1489, 2202 |
| Bse1I | ACTGG | 4 cut(s) | 666, 807, 1015, 1442 |
| Bse3DI | GCAATG | 1 cut(s) | 952 |
| Bse8I | GATNNNNATC | 1 cut(s) | 318 |
| BseBI | CCWGG | 1 cut(s) | 1759 |
| BseDI | CCNNGG | 6 cut(s) | 48, 334, 1051, 1182, 1261, 1758 |
| BseGI | GGATG | 5 cut(s) | 559, 933, 1429, 1681, 1906 |
| BseJI | GATNNNNATC | 1 cut(s) | 318 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 825, 908, 1489, 2202 |
| BseMI | GCAATG | 1 cut(s) | 952 |
| BseMII | CTCAG | 7 cut(s) | 165, 290, 1183, 1235, 1875, 1979, 2052 |
| BseNI | ACTGG | 4 cut(s) | 666, 807, 1015, 1442 |
| BseRI | GAGGAG | 2 cut(s) | 239, 866 |
| BseSI | GKGCMC | 1 cut(s) | 661 |
| BseYI | CCCAGC | 2 cut(s) | 745, 1628 |
| BsgI | GTGCAG | 6 cut(s) | 615, 620, 816, 852, 908, 1641 |
| Bsh1236I | CGCG | 2 cut(s) | 344, 789 |
| BshFI | GGCC | 7 cut(s) | 47, 200, 369, 1181, 1376, 1805, 2195 |
| BshNI | GGYRCC | 3 cut(s) | 146, 980, 1772 |
| BsiHKAI | GWGCWC | 1 cut(s) | 661 |
| BsiHKCI | CYCGRG | 2 cut(s) | 383, 1935 |
| BsiSI | CCGG | 5 cut(s) | 15, 32, 84, 1483, 2078 |
| BslFI | GGGAC | 5 cut(s) | 203, 776, 1243, 1682, 1703 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 825, 908, 1489, 2202 |
| BsmAI | GTCTC | 3 cut(s) | 1118, 1172, 1529 |
| BsmFI | GGGAC | 5 cut(s) | 203, 776, 1243, 1682, 1703 |
| BsmI | GAATGC | 2 cut(s) | 1478, 1828 |
| BsnI | GGCC | 7 cut(s) | 47, 200, 369, 1181, 1376, 1805, 2195 |
| BsoBI | CYCGRG | 2 cut(s) | 383, 1935 |
| Bsp1286I | GDGCHC | 2 cut(s) | 661, 1936 |
| Bsp1407I | TGTACA | 1 cut(s) | 514 |
| Bsp143I | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 299, 1884 |
| Bsp19I | CCATGG | 1 cut(s) | 1182 |
| Bsp68I | TCGCGA | 1 cut(s) | 344 |
| BspACI | CCGC | 6 cut(s) | 114, 187, 499, 909, 1048, 1959 |
| BspANI | GGCC | 7 cut(s) | 47, 200, 369, 1181, 1376, 1805, 2195 |
| BspCNI | CTCAG | 7 cut(s) | 166, 291, 1182, 1234, 1876, 1980, 2053 |
| BspFNI | CGCG | 2 cut(s) | 344, 789 |
| BspHI | TCATGA | 4 cut(s) | 570, 1446, 1818, 1975 |
| BspLI | GGNNCC | 5 cut(s) | 148, 982, 1259, 1774, 1804 |
| BspMAI | CTGCAG | 2 cut(s) | 542, 673 |
| BspMI | ACCTGC | 1 cut(s) | 676 |
| BspOI | GCTAGC | 1 cut(s) | 2076 |
| BspPI | GGATC | 3 cut(s) | 113, 382, 490 |
| BspQI | GCTCTTC | 1 cut(s) | 335 |
| BspT107I | GGYRCC | 3 cut(s) | 146, 980, 1772 |
| BsrDI | GCAATG | 1 cut(s) | 952 |
| BsrGI | TGTACA | 1 cut(s) | 514 |
| BsrI | ACTGG | 4 cut(s) | 666, 807, 1015, 1442 |
| BssECI | CCNNGG | 6 cut(s) | 48, 334, 1051, 1182, 1261, 1758 |
| BssMI | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| BssNI | GRCGYC | 3 cut(s) | 132, 147, 240 |
| BssT1I | CCWWGG | 3 cut(s) | 1051, 1182, 1261 |
| Bst2UI | CCWGG | 1 cut(s) | 1759 |
| Bst4CI | ACNGT | 4 cut(s) | 7, 653, 697, 1571 |
| Bst6I | CTCTTC | 4 cut(s) | 257, 335, 774, 1155 |
| BstACI | GRCGYC | 3 cut(s) | 132, 147, 240 |
| BstAUI | TGTACA | 1 cut(s) | 514 |
| BstBAI | YACGTR | 1 cut(s) | 1928 |
| BstDEI | CTNAG | 8 cut(s) | 174, 299, 624, 1169, 1221, 1884, 1988, 2061 |
| BstDSI | CCRYGG | 2 cut(s) | 334, 1182 |
| BstF5I | GGATG | 5 cut(s) | 559, 933, 1429, 1681, 1906 |
| BstFNI | CGCG | 2 cut(s) | 344, 789 |
| BstH2I | RGCGCY | 2 cut(s) | 64, 150 |
| BstHHI | GCGC | 5 cut(s) | 63, 113, 149, 789, 967 |
| BstKTI | GATC | 8 cut(s) | 91, 121, 390, 498, 1140, 1452, 1794, 1987 |
| BstMAI | GTCTC | 3 cut(s) | 1118, 1172, 1529 |
| BstMBI | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| BstNI | CCWGG | 1 cut(s) | 1759 |
| BstNSI | RCATGY | 2 cut(s) | 521, 537 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 731 |
| BstSCI | CCNGG | 3 cut(s) | 30, 1757, 2076 |
| BstSFI | CTRYAG | 5 cut(s) | 538, 652, 669, 771, 1386 |
| BstSLI | GKGCMC | 1 cut(s) | 661 |
| BstUI | CGCG | 2 cut(s) | 344, 789 |
| BstV2I | GAAGAC | 1 cut(s) | 1022 |
| BstX2I | RGATCY | 3 cut(s) | 88, 387, 1984 |
| BstYI | RGATCY | 3 cut(s) | 88, 387, 1984 |
| BsuI | GTATCC | 1 cut(s) | 600 |
| BsuRI | GGCC | 7 cut(s) | 47, 200, 369, 1181, 1376, 1805, 2195 |
| BtgI | CCRYGG | 2 cut(s) | 334, 1182 |
| BtgZI | GCGATG | 2 cut(s) | 116, 2032 |
| BtrI | CACGTC | 1 cut(s) | 1661 |
| BtsCI | GGATG | 5 cut(s) | 559, 933, 1429, 1681, 1906 |
| BtsI | GCAGTG | 3 cut(s) | 363, 840, 1452 |
| BtsIMutI | CAGTG | 4 cut(s) | 363, 659, 840, 1452 |
| BtuMI | TCGCGA | 1 cut(s) | 344 |
| BveI | ACCTGC | 1 cut(s) | 676 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1118, 1505 |
| CciI | TCATGA | 4 cut(s) | 570, 1446, 1818, 1975 |
| CfoI | GCGC | 5 cut(s) | 63, 113, 149, 789, 967 |
| Cfr13I | GGNCC | 3 cut(s) | 367, 1257, 1803 |
| Csp6I | GTAC | 2 cut(s) | 515, 1773 |
| CviQI | GTAC | 2 cut(s) | 515, 1773 |
| DdeI | CTNAG | 8 cut(s) | 174, 299, 624, 1169, 1221, 1884, 1988, 2061 |
| DinI | GGCGCC | 1 cut(s) | 148 |
| DpnI | GATC | 8 cut(s) | 90, 120, 389, 497, 1139, 1451, 1793, 1986 |
| DpnII | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| DraIII | CACNNNGTG | 1 cut(s) | 1664 |
| EaeI | YGGCCR | 3 cut(s) | 198, 1179, 2193 |
| Eam1104I | CTCTTC | 4 cut(s) | 257, 335, 774, 1155 |
| EarI | CTCTTC | 4 cut(s) | 257, 335, 774, 1155 |
| EciI | GGCGGA | 1 cut(s) | 488 |
| Eco130I | CCWWGG | 3 cut(s) | 1051, 1182, 1261 |
| Eco147I | AGGCCT | 1 cut(s) | 1376 |
| Eco24I | GRGCYC | 1 cut(s) | 1936 |
| Eco47I | GGWCC | 1 cut(s) | 1257 |
| Eco57I | CTGAAG | 2 cut(s) | 113, 599 |
| Eco88I | CYCGRG | 2 cut(s) | 383, 1935 |
| EcoRII | CCWGG | 1 cut(s) | 1757 |
| EcoT14I | CCWWGG | 3 cut(s) | 1051, 1182, 1261 |
| EcoT38I | GRGCYC | 1 cut(s) | 1936 |
| EgeI | GGCGCC | 1 cut(s) | 148 |
| EheI | GGCGCC | 1 cut(s) | 148 |
| ErhI | CCWWGG | 3 cut(s) | 1051, 1182, 1261 |
| FaqI | GGGAC | 5 cut(s) | 203, 776, 1243, 1682, 1703 |
| FauI | CCCGC | 3 cut(s) | 180, 1055, 1952 |
| FbaI | TGATCA | 1 cut(s) | 1449 |
| FblI | GTMKAC | 2 cut(s) | 129, 648 |
| FokI | GGATG | 5 cut(s) | 566, 920, 1436, 1688, 1913 |
| FriOI | GRGCYC | 1 cut(s) | 1936 |
| FspBI | CTAG | 3 cut(s) | 852, 1811, 2073 |
| GlaI | GCGC | 5 cut(s) | 62, 112, 148, 788, 966 |
| GsaI | CCCAGC | 2 cut(s) | 749, 1632 |
| GsuI | CTGGAG | 2 cut(s) | 423, 2145 |
| HaeII | RGCGCY | 2 cut(s) | 64, 150 |
| HaeIII | GGCC | 7 cut(s) | 47, 200, 369, 1181, 1376, 1805, 2195 |
| HapII | CCGG | 5 cut(s) | 15, 32, 84, 1483, 2078 |
| HhaI | GCGC | 5 cut(s) | 63, 113, 149, 789, 967 |
| Hin1I | GRCGYC | 3 cut(s) | 132, 147, 240 |
| Hin6I | GCGC | 5 cut(s) | 61, 111, 147, 787, 965 |
| HinP1I | GCGC | 5 cut(s) | 61, 111, 147, 787, 965 |
| HincII | GTYRAC | 5 cut(s) | 130, 136, 562, 649, 1692 |
| HindII | GTYRAC | 5 cut(s) | 130, 136, 562, 649, 1692 |
| HinfI | GANTC | 8 cut(s) | 194, 213, 379, 992, 1115, 1296, 1394, 1894 |
| HpaI | GTTAAC | 1 cut(s) | 1692 |
| HpaII | CCGG | 5 cut(s) | 15, 32, 84, 1483, 2078 |
| HphI | GGTGA | 7 cut(s) | 298, 1133, 1376, 1544, 1600, 2077, 2218 |
| Hpy166II | GTNNAC | 8 cut(s) | 37, 130, 136, 562, 649, 659, 1692, 1784 |
| Hpy188I | TCNGA | 7 cut(s) | 93, 212, 324, 490, 1172, 1989, 2037 |
| Hpy8I | GTNNAC | 8 cut(s) | 37, 130, 136, 562, 649, 659, 1692, 1784 |
| Hpy99I | CGWCG | 5 cut(s) | 131, 134, 146, 260, 1665 |
| HpyAV | CCTTC | 7 cut(s) | 74, 639, 1079, 1579, 1757, 2000, 2171 |
| HpyCH4III | ACNGT | 4 cut(s) | 7, 653, 697, 1571 |
| HpyCH4IV | ACGT | 6 cut(s) | 132, 240, 468, 1470, 1660, 1927 |
| HpyF3I | CTNAG | 8 cut(s) | 174, 299, 624, 1169, 1221, 1884, 1988, 2061 |
| HpySE526I | ACGT | 6 cut(s) | 132, 240, 468, 1470, 1660, 1927 |
| Hsp92I | GRCGYC | 3 cut(s) | 132, 147, 240 |
| HspAI | GCGC | 5 cut(s) | 61, 111, 147, 787, 965 |
| KasI | GGCGCC | 1 cut(s) | 146 |
| KpnI | GGTACC | 1 cut(s) | 1776 |
| Ksp22I | TGATCA | 1 cut(s) | 1449 |
| KspAI | GTTAAC | 1 cut(s) | 1692 |
| Kzo9I | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| LguI | GCTCTTC | 1 cut(s) | 335 |
| LmnI | GCTCC | 8 cut(s) | 308, 404, 472, 749, 879, 953, 1502, 2100 |
| LweI | GCATC | 5 cut(s) | 416, 577, 1581, 1666, 1870 |
| MaeI | CTAG | 3 cut(s) | 852, 1811, 2073 |
| MaeII | ACGT | 6 cut(s) | 132, 240, 468, 1470, 1660, 1927 |
| MaeIII | GTNAC | 9 cut(s) | 218, 241, 1243, 1466, 1571, 1703, 1733, 2140, 2224 |
| MalI | GATC | 8 cut(s) | 90, 120, 389, 497, 1139, 1451, 1793, 1986 |
| MboI | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| MboII | GAAGA | 7 cut(s) | 244, 301, 322, 791, 924, 1027, 1172 |
| MfeI | CAATTG | 1 cut(s) | 1236 |
| MflI | RGATCY | 3 cut(s) | 88, 387, 1984 |
| MhlI | GDGCHC | 2 cut(s) | 661, 1936 |
| MlsI | TGGCCA | 1 cut(s) | 1181 |
| MluCI | AATT | 6 cut(s) | 281, 293, 1236, 1403, 1542, 1598 |
| MluNI | TGGCCA | 1 cut(s) | 1181 |
| Mly113I | GGCGCC | 1 cut(s) | 147 |
| MlyI | GAGTC | 2 cut(s) | 222, 1109 |
| MmeI | TCCRAC | 1 cut(s) | 417 |
| Mox20I | TGGCCA | 1 cut(s) | 1181 |
| MroXI | GAANNNNTTC | 1 cut(s) | 329 |
| MscI | TGGCCA | 1 cut(s) | 1181 |
| MseI | TTAA | 4 cut(s) | 396, 1002, 1559, 1691 |
| MslI | CAYNNNNRTG | 5 cut(s) | 456, 569, 642, 757, 978 |
| Msp20I | TGGCCA | 1 cut(s) | 1181 |
| MspI | CCGG | 5 cut(s) | 15, 32, 84, 1483, 2078 |
| MspR9I | CCNGG | 3 cut(s) | 32, 1759, 2078 |
| MunI | CAATTG | 1 cut(s) | 1236 |
| Mva1269I | GAATGC | 2 cut(s) | 1478, 1828 |
| MvaI | CCWGG | 1 cut(s) | 1759 |
| MvnI | CGCG | 2 cut(s) | 344, 789 |
| NarI | GGCGCC | 1 cut(s) | 147 |
| NciI | CCSGG | 2 cut(s) | 32, 2078 |
| NcoI | CCATGG | 1 cut(s) | 1182 |
| NdeII | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| NheI | GCTAGC | 1 cut(s) | 2072 |
| NlaIV | GGNNCC | 5 cut(s) | 148, 982, 1259, 1774, 1804 |
| NmeAIII | GCCGAG | 3 cut(s) | 73, 2078, 2221 |
| NmuCI | GTSAC | 6 cut(s) | 241, 1243, 1466, 1571, 2140, 2224 |
| NruI | TCGCGA | 1 cut(s) | 344 |
| NspI | RCATGY | 2 cut(s) | 521, 537 |
| PaeI | GCATGC | 1 cut(s) | 537 |
| PaeR7I | CTCGAG | 1 cut(s) | 383 |
| PagI | TCATGA | 4 cut(s) | 570, 1446, 1818, 1975 |
| PceI | AGGCCT | 1 cut(s) | 1376 |
| PciSI | GCTCTTC | 1 cut(s) | 335 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 138, 147 |
| PctI | GAATGC | 2 cut(s) | 1478, 1828 |
| PdmI | GAANNNNTTC | 1 cut(s) | 329 |
| PfeI | GAWTC | 6 cut(s) | 194, 379, 992, 1296, 1394, 1894 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 825 |
| PleI | GAGTC | 2 cut(s) | 221, 1109 |
| PluTI | GGCGCC | 1 cut(s) | 150 |
| PpsI | GAGTC | 2 cut(s) | 221, 1109 |
| Ppu21I | YACGTR | 1 cut(s) | 1928 |
| PshAI | GACNNNNGTC | 1 cut(s) | 731 |
| Psp6I | CCWGG | 1 cut(s) | 1757 |
| PspFI | CCCAGC | 2 cut(s) | 745, 1628 |
| PspGI | CCWGG | 1 cut(s) | 1757 |
| PspN4I | GGNNCC | 5 cut(s) | 148, 982, 1259, 1774, 1804 |
| PspPI | GGNCC | 3 cut(s) | 367, 1257, 1803 |
| PstI | CTGCAG | 2 cut(s) | 542, 673 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1118, 1505 |
| PsuI | RGATCY | 3 cut(s) | 88, 387, 1984 |
| RruI | TCGCGA | 1 cut(s) | 344 |
| RsaI | GTAC | 2 cut(s) | 516, 1774 |
| RsaNI | GTAC | 2 cut(s) | 515, 1773 |
| RseI | CAYNNNNRTG | 5 cut(s) | 456, 569, 642, 757, 978 |
| SalI | GTCGAC | 2 cut(s) | 128, 647 |
| SapI | GCTCTTC | 1 cut(s) | 335 |
| SaqAI | TTAA | 4 cut(s) | 396, 1002, 1559, 1691 |
| Sau3AI | GATC | 8 cut(s) | 88, 118, 387, 495, 1137, 1449, 1791, 1984 |
| Sau96I | GGNCC | 3 cut(s) | 367, 1257, 1803 |
| SchI | GAGTC | 2 cut(s) | 222, 1109 |
| ScrFI | CCNGG | 3 cut(s) | 32, 1759, 2078 |
| SduI | GDGCHC | 2 cut(s) | 661, 1936 |
| SfaNI | GCATC | 5 cut(s) | 416, 577, 1581, 1666, 1870 |
| SfcI | CTRYAG | 5 cut(s) | 538, 652, 669, 771, 1386 |
| SfoI | GGCGCC | 1 cut(s) | 148 |
| Sfr274I | CTCGAG | 1 cut(s) | 383 |
| SgrDI | CGTCGACG | 1 cut(s) | 128 |
| SinI | GGWCC | 1 cut(s) | 1257 |
| SlaI | CTCGAG | 1 cut(s) | 383 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 456, 569, 642, 757, 978 |
| SmlI | CTYRAG | 3 cut(s) | 383, 1356, 1943 |
| SmoI | CTYRAG | 3 cut(s) | 383, 1356, 1943 |
| SphI | GCATGC | 1 cut(s) | 537 |
| Sse9I | AATT | 6 cut(s) | 281, 293, 1236, 1403, 1542, 1598 |
| SseBI | AGGCCT | 1 cut(s) | 1376 |
| SsiI | CCGC | 6 cut(s) | 114, 187, 499, 909, 1048, 1959 |
| SspDI | GGCGCC | 1 cut(s) | 146 |
| SspI | AATATT | 1 cut(s) | 886 |
| SspMI | CTAG | 3 cut(s) | 852, 1811, 2073 |
| StuI | AGGCCT | 1 cut(s) | 1376 |
| StyD4I | CCNGG | 3 cut(s) | 30, 1757, 2076 |
| StyI | CCWWGG | 3 cut(s) | 1051, 1182, 1261 |
| TaaI | ACNGT | 4 cut(s) | 7, 653, 697, 1571 |
| TaiI | ACGT | 6 cut(s) | 135, 243, 471, 1473, 1663, 1930 |
| TaqI | TCGA | 6 cut(s) | 129, 384, 585, 600, 648, 1608 |
| TasI | AATT | 6 cut(s) | 281, 293, 1236, 1403, 1542, 1598 |
| TatI | WGTACW | 1 cut(s) | 514 |
| TauI | GCSGC | 1 cut(s) | 116 |
| TfiI | GAWTC | 6 cut(s) | 194, 379, 992, 1296, 1394, 1894 |
| Tru1I | TTAA | 4 cut(s) | 396, 1002, 1559, 1691 |
| Tru9I | TTAA | 4 cut(s) | 396, 1002, 1559, 1691 |
| TscAI | CASTG | 4 cut(s) | 363, 666, 840, 1459 |
| TseFI | GTSAC | 6 cut(s) | 241, 1243, 1466, 1571, 2140, 2224 |
| Tsp45I | GTSAC | 6 cut(s) | 241, 1243, 1466, 1571, 2140, 2224 |
| TspDTI | ATGAA | 6 cut(s) | 254, 587, 1173, 1835, 1917, 2133 |
| TspGWI | ACGGA | 2 cut(s) | 323, 689 |
| TspRI | CASTG | 4 cut(s) | 363, 666, 840, 1459 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 825 |
| VneI | GTGCAC | 1 cut(s) | 657 |
| VpaK11BI | GGWCC | 1 cut(s) | 1257 |
| XapI | RAATTY | 2 cut(s) | 1403, 1598 |
| XceI | RCATGY | 2 cut(s) | 521, 537 |
| XhoI | CTCGAG | 1 cut(s) | 383 |
| XmiI | GTMKAC | 2 cut(s) | 129, 648 |
| XmnI | GAANNNNTTC | 1 cut(s) | 329 |
| XspI | CTAG | 3 cut(s) | 852, 1811, 2073 |
| ZraI | GACGTC | 2 cut(s) | 133, 241 |