Belongs to the cysteine synthase cystathionine beta- synthase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AccI | GTMKAC | 1 cut(s) | 174 |
| AciI | CCGC | 4 cut(s) | 204, 219, 356, 417 |
| AclWI | GGATC | 1 cut(s) | 425 |
| AcoI | YGGCCR | 1 cut(s) | 414 |
| AcyI | GRCGYC | 1 cut(s) | 261 |
| AfaI | GTAC | 2 cut(s) | 63, 304 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 283, 447, 529, 701 |
| AgsI | TTSAA | 2 cut(s) | 338, 580 |
| AjiI | CACGTC | 1 cut(s) | 516 |
| AjnI | CCWGG | 2 cut(s) | 528, 688 |
| AluBI | AGCT | 2 cut(s) | 243, 472 |
| AluI | AGCT | 2 cut(s) | 243, 472 |
| Alw26I | GTCTC | 1 cut(s) | 741 |
| AlwI | GGATC | 1 cut(s) | 425 |
| AoxI | GGCC | 5 cut(s) | 15, 414, 692, 703, 731 |
| ApeKI | GCWGC | 3 cut(s) | 240, 287, 469 |
| AspA2I | CCTAGG | 1 cut(s) | 700 |
| AspLEI | GCGC | 1 cut(s) | 224 |
| AspS9I | GGNCC | 4 cut(s) | 38, 274, 520, 692 |
| AsuC2I | CCSGG | 1 cut(s) | 326 |
| AsuHPI | GGTGA | 3 cut(s) | 419, 511, 629 |
| AvaII | GGWCC | 3 cut(s) | 38, 274, 520 |
| AvrII | CCTAGG | 1 cut(s) | 700 |
| AxyI | CCTNAGG | 2 cut(s) | 728, 735 |
| BaeGI | GKGCMC | 1 cut(s) | 9 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BauI | CACGAG | 2 cut(s) | 168, 476 |
| BbvI | GCAGC | 3 cut(s) | 252, 299, 481 |
| BccI | CCATC | 1 cut(s) | 608 |
| BceAI | ACGGC | 3 cut(s) | 232, 538, 679 |
| BciT130I | CCWGG | 2 cut(s) | 530, 690 |
| BcnI | CCSGG | 1 cut(s) | 326 |
| BcoDI | GTCTC | 1 cut(s) | 741 |
| BfaI | CTAG | 1 cut(s) | 701 |
| BfoI | RGCGCY | 1 cut(s) | 225 |
| BisI | GCNGC | 5 cut(s) | 220, 241, 288, 417, 470 |
| BlnI | CCTAGG | 1 cut(s) | 700 |
| BlsI | GCNGC | 5 cut(s) | 221, 242, 289, 418, 471 |
| Bme1390I | CCNGG | 3 cut(s) | 326, 530, 690 |
| Bme18I | GGWCC | 3 cut(s) | 38, 274, 520 |
| BmgBI | CACGTC | 1 cut(s) | 516 |
| BmgT120I | GGNCC | 4 cut(s) | 38, 274, 520, 692 |
| BmiI | GGNNCC | 2 cut(s) | 6, 522 |
| BmrFI | CCNGG | 3 cut(s) | 326, 530, 690 |
| BmsI | GCATC | 3 cut(s) | 85, 364, 460 |
| BpuMI | CCSGG | 1 cut(s) | 326 |
| BsaBI | GATNNNNATC | 1 cut(s) | 632 |
| BsaHI | GRCGYC | 1 cut(s) | 261 |
| BsaI | GGTCTC | 1 cut(s) | 741 |
| BsaJI | CCNNGG | 5 cut(s) | 387, 528, 689, 700, 706 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 283, 447, 529, 701 |
| Bse1I | ACTGG | 1 cut(s) | 241 |
| Bse21I | CCTNAGG | 2 cut(s) | 728, 735 |
| Bse8I | GATNNNNATC | 1 cut(s) | 632 |
| BseBI | CCWGG | 2 cut(s) | 530, 690 |
| BseDI | CCNNGG | 5 cut(s) | 387, 528, 689, 700, 706 |
| BseGI | GGATG | 3 cut(s) | 100, 658, 685 |
| BseJI | GATNNNNATC | 1 cut(s) | 632 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 283, 447, 529, 701 |
| BseMII | CTCAG | 2 cut(s) | 258, 749 |
| BseNI | ACTGG | 1 cut(s) | 241 |
| BseSI | GKGCMC | 1 cut(s) | 9 |
| BseXI | GCAGC | 3 cut(s) | 252, 299, 481 |
| BshFI | GGCC | 5 cut(s) | 17, 416, 694, 705, 733 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BsiSI | CCGG | 1 cut(s) | 326 |
| BslFI | GGGAC | 1 cut(s) | 511 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 283, 447, 529, 701 |
| BsmAI | GTCTC | 1 cut(s) | 741 |
| BsmFI | GGGAC | 1 cut(s) | 511 |
| BsnI | GGCC | 5 cut(s) | 17, 416, 694, 705, 733 |
| Bso31I | GGTCTC | 1 cut(s) | 741 |
| Bsp1286I | GDGCHC | 1 cut(s) | 9 |
| Bsp143I | GATC | 2 cut(s) | 430, 633 |
| BspACI | CCGC | 4 cut(s) | 204, 219, 356, 417 |
| BspANI | GGCC | 5 cut(s) | 17, 416, 694, 705, 733 |
| BspCNI | CTCAG | 2 cut(s) | 257, 748 |
| BspLI | GGNNCC | 2 cut(s) | 6, 522 |
| BspPI | GGATC | 1 cut(s) | 425 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BspTNI | GGTCTC | 1 cut(s) | 741 |
| BsrI | ACTGG | 1 cut(s) | 241 |
| BssECI | CCNNGG | 5 cut(s) | 387, 528, 689, 700, 706 |
| BssMI | GATC | 2 cut(s) | 430, 633 |
| BssNI | GRCGYC | 1 cut(s) | 261 |
| BssSI | CACGAG | 2 cut(s) | 168, 476 |
| BssT1I | CCWWGG | 3 cut(s) | 387, 700, 706 |
| Bst2BI | CACGAG | 2 cut(s) | 168, 476 |
| Bst2UI | CCWGG | 2 cut(s) | 530, 690 |
| Bst4CI | ACNGT | 1 cut(s) | 424 |
| Bst6I | CTCTTC | 1 cut(s) | 642 |
| BstACI | GRCGYC | 1 cut(s) | 261 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 383 |
| BstC8I | GCNNGC | 3 cut(s) | 165, 449, 663 |
| BstDEI | CTNAG | 3 cut(s) | 244, 728, 735 |
| BstF5I | GGATG | 3 cut(s) | 100, 658, 685 |
| BstH2I | RGCGCY | 1 cut(s) | 225 |
| BstHHI | GCGC | 1 cut(s) | 224 |
| BstKTI | GATC | 2 cut(s) | 433, 636 |
| BstMAI | GTCTC | 1 cut(s) | 741 |
| BstMBI | GATC | 2 cut(s) | 430, 633 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 383, 717 |
| BstNI | CCWGG | 2 cut(s) | 530, 690 |
| BstNSI | RCATGY | 1 cut(s) | 258 |
| BstSCI | CCNGG | 3 cut(s) | 324, 528, 688 |
| BstSLI | GKGCMC | 1 cut(s) | 9 |
| BstV1I | GCAGC | 3 cut(s) | 252, 299, 481 |
| Bsu36I | CCTNAGG | 2 cut(s) | 728, 735 |
| BsuRI | GGCC | 5 cut(s) | 17, 416, 694, 705, 733 |
| BtrI | CACGTC | 1 cut(s) | 516 |
| BtsCI | GGATG | 3 cut(s) | 100, 658, 685 |
| Cac8I | GCNNGC | 3 cut(s) | 165, 449, 663 |
| CfoI | GCGC | 1 cut(s) | 224 |
| Cfr13I | GGNCC | 4 cut(s) | 38, 274, 520, 692 |
| CseI | GACGC | 1 cut(s) | 250 |
| Csp6I | GTAC | 2 cut(s) | 62, 303 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 409, 444 |
| CviAII | CATG | 4 cut(s) | 187, 255, 282, 291 |
| CviQI | GTAC | 2 cut(s) | 62, 303 |
| DdeI | CTNAG | 3 cut(s) | 244, 728, 735 |
| DpnI | GATC | 2 cut(s) | 432, 635 |
| DpnII | GATC | 2 cut(s) | 430, 633 |
| EaeI | YGGCCR | 1 cut(s) | 414 |
| Eam1104I | CTCTTC | 1 cut(s) | 642 |
| EarI | CTCTTC | 1 cut(s) | 642 |
| Eco130I | CCWWGG | 3 cut(s) | 387, 700, 706 |
| Eco147I | AGGCCT | 1 cut(s) | 733 |
| Eco31I | GGTCTC | 1 cut(s) | 741 |
| Eco47I | GGWCC | 3 cut(s) | 38, 274, 520 |
| Eco81I | CCTNAGG | 2 cut(s) | 728, 735 |
| EcoRII | CCWGG | 2 cut(s) | 528, 688 |
| EcoT14I | CCWWGG | 3 cut(s) | 387, 700, 706 |
| ErhI | CCWWGG | 3 cut(s) | 387, 700, 706 |
| FaeI | CATG | 4 cut(s) | 190, 258, 285, 294 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 371, 403 |
| FaqI | GGGAC | 1 cut(s) | 511 |
| FatI | CATG | 4 cut(s) | 186, 254, 281, 290 |
| FblI | GTMKAC | 1 cut(s) | 174 |
| Fnu4HI | GCNGC | 5 cut(s) | 220, 241, 288, 417, 470 |
| FokI | GGATG | 3 cut(s) | 107, 665, 672 |
| Fsp4HI | GCNGC | 5 cut(s) | 220, 241, 288, 417, 470 |
| FspBI | CTAG | 1 cut(s) | 701 |
| GlaI | GCGC | 1 cut(s) | 223 |
| GluI | GCNGC | 5 cut(s) | 220, 241, 288, 417, 470 |
| HaeII | RGCGCY | 1 cut(s) | 225 |
| HaeIII | GGCC | 5 cut(s) | 17, 416, 694, 705, 733 |
| HapII | CCGG | 1 cut(s) | 326 |
| HgaI | GACGC | 1 cut(s) | 250 |
| HhaI | GCGC | 1 cut(s) | 224 |
| Hin1I | GRCGYC | 1 cut(s) | 261 |
| Hin1II | CATG | 4 cut(s) | 190, 258, 285, 294 |
| Hin6I | GCGC | 1 cut(s) | 222 |
| HinP1I | GCGC | 1 cut(s) | 222 |
| HinfI | GANTC | 3 cut(s) | 86, 346, 576 |
| HpaII | CCGG | 1 cut(s) | 326 |
| HphI | GGTGA | 3 cut(s) | 419, 511, 629 |
| Hpy166II | GTNNAC | 2 cut(s) | 175, 502 |
| Hpy188I | TCNGA | 5 cut(s) | 121, 351, 404, 520, 638 |
| Hpy188III | TCNNGA | 1 cut(s) | 476 |
| Hpy8I | GTNNAC | 2 cut(s) | 175, 502 |
| Hpy99I | CGWCG | 1 cut(s) | 520 |
| HpyAV | CCTTC | 1 cut(s) | 134 |
| HpyCH4III | ACNGT | 1 cut(s) | 424 |
| HpyCH4IV | ACGT | 1 cut(s) | 515 |
| HpyCH4V | TGCA | 1 cut(s) | 377 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 383, 717 |
| HpyF3I | CTNAG | 3 cut(s) | 244, 728, 735 |
| HpySE526I | ACGT | 1 cut(s) | 515 |
| Hsp92I | GRCGYC | 1 cut(s) | 261 |
| Hsp92II | CATG | 4 cut(s) | 190, 258, 285, 294 |
| HspAI | GCGC | 1 cut(s) | 222 |
| Kzo9I | GATC | 2 cut(s) | 430, 633 |
| Lsp1109I | GCAGC | 3 cut(s) | 252, 299, 481 |
| LweI | GCATC | 3 cut(s) | 85, 364, 460 |
| MaeI | CTAG | 1 cut(s) | 701 |
| MaeII | ACGT | 1 cut(s) | 515 |
| MalI | GATC | 2 cut(s) | 432, 635 |
| MboI | GATC | 2 cut(s) | 430, 633 |
| MboII | GAAGA | 1 cut(s) | 659 |
| MhlI | GDGCHC | 1 cut(s) | 9 |
| MlyI | GAGTC | 1 cut(s) | 355 |
| MmeI | TCCRAC | 1 cut(s) | 498 |
| MnlI | CCTC | 8 cut(s) | 28, 265, 398, 445, 494, 643, 744, 761 |
| MseI | TTAA | 2 cut(s) | 309, 630 |
| MslI | CAYNNNNRTG | 1 cut(s) | 135 |
| MspA1I | CMGCKG | 1 cut(s) | 204 |
| MspI | CCGG | 1 cut(s) | 326 |
| MspR9I | CCNGG | 3 cut(s) | 326, 530, 690 |
| MvaI | CCWGG | 2 cut(s) | 530, 690 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 383, 717 |
| NciI | CCSGG | 1 cut(s) | 326 |
| NdeII | GATC | 2 cut(s) | 430, 633 |
| NlaIII | CATG | 4 cut(s) | 190, 258, 285, 294 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 522 |
| NspI | RCATGY | 1 cut(s) | 258 |
| PceI | AGGCCT | 1 cut(s) | 733 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 521 |
| PfeI | GAWTC | 2 cut(s) | 86, 576 |
| PfoI | TCCNGGA | 1 cut(s) | 324 |
| PkrI | GCNGC | 5 cut(s) | 221, 242, 289, 418, 471 |
| PleI | GAGTC | 1 cut(s) | 354 |
| PpsI | GAGTC | 1 cut(s) | 354 |
| Psp6I | CCWGG | 2 cut(s) | 528, 688 |
| PspGI | CCWGG | 2 cut(s) | 528, 688 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 522 |
| PspPI | GGNCC | 4 cut(s) | 38, 274, 520, 692 |
| RsaI | GTAC | 2 cut(s) | 63, 304 |
| RsaNI | GTAC | 2 cut(s) | 62, 303 |
| RseI | CAYNNNNRTG | 1 cut(s) | 135 |
| SaqAI | TTAA | 2 cut(s) | 309, 630 |
| SatI | GCNGC | 5 cut(s) | 220, 241, 288, 417, 470 |
| Sau3AI | GATC | 2 cut(s) | 430, 633 |
| Sau96I | GGNCC | 4 cut(s) | 38, 274, 520, 692 |
| SchI | GAGTC | 1 cut(s) | 355 |
| ScrFI | CCNGG | 3 cut(s) | 326, 530, 690 |
| SduI | GDGCHC | 1 cut(s) | 9 |
| SetI | ASST | 9 cut(s) | 180, 245, 315, 319, 409, 474, 486, 518, 753 |
| SfaNI | GCATC | 3 cut(s) | 85, 364, 460 |
| SinI | GGWCC | 3 cut(s) | 38, 274, 520 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 135 |
| SseBI | AGGCCT | 1 cut(s) | 733 |
| SsiI | CCGC | 4 cut(s) | 204, 219, 356, 417 |
| SspMI | CTAG | 1 cut(s) | 701 |
| StuI | AGGCCT | 1 cut(s) | 733 |
| StyD4I | CCNGG | 3 cut(s) | 324, 528, 688 |
| StyI | CCWWGG | 3 cut(s) | 387, 700, 706 |
| TaaI | ACNGT | 1 cut(s) | 424 |
| TaiI | ACGT | 1 cut(s) | 518 |
| TaqI | TCGA | 2 cut(s) | 454, 612 |
| TatI | WGTACW | 2 cut(s) | 61, 302 |
| TauI | GCSGC | 2 cut(s) | 222, 419 |
| TfiI | GAWTC | 2 cut(s) | 86, 576 |
| Tru1I | TTAA | 2 cut(s) | 309, 630 |
| Tru9I | TTAA | 2 cut(s) | 309, 630 |
| TseI | GCWGC | 3 cut(s) | 240, 287, 469 |
| TspDTI | ATGAA | 2 cut(s) | 119, 153 |
| TspGWI | ACGGA | 1 cut(s) | 117 |
| VpaK11BI | GGWCC | 3 cut(s) | 38, 274, 520 |
| XceI | RCATGY | 1 cut(s) | 258 |
| XmaJI | CCTAGG | 1 cut(s) | 700 |
| XmiI | GTMKAC | 1 cut(s) | 174 |
| XspI | CTAG | 1 cut(s) | 701 |