Belongs to the cysteine synthase cystathionine beta- synthase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1028 |
| AccB1I | GGYRCC | 1 cut(s) | 1014 |
| AciI | CCGC | 5 cut(s) | 185, 242, 816, 907, 1428 |
| AclWI | GGATC | 3 cut(s) | 433, 669, 1484 |
| AcoI | YGGCCR | 1 cut(s) | 1425 |
| AcsI | RAATTY | 2 cut(s) | 474, 1316 |
| AcuI | CTGAAG | 1 cut(s) | 143 |
| AfaI | GTAC | 1 cut(s) | 1070 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 463, 874 |
| AlwI | GGATC | 3 cut(s) | 433, 669, 1484 |
| AoxI | GGCC | 8 cut(s) | 295, 607, 803, 976, 1055, 1129, 1143, 1425 |
| ApeKI | GCWGC | 4 cut(s) | 314, 533, 1472, 1475 |
| ApoI | RAATTY | 2 cut(s) | 474, 1316 |
| Asp700I | GAANNNNTTC | 3 cut(s) | 8, 456, 501 |
| AspA2I | CCTAGG | 1 cut(s) | 868 |
| AspLEI | GCGC | 1 cut(s) | 17 |
| AspS9I | GGNCC | 4 cut(s) | 608, 766, 976, 1056 |
| AsuC2I | CCSGG | 1 cut(s) | 388 |
| AsuHPI | GGTGA | 3 cut(s) | 344, 955, 1496 |
| AvaII | GGWCC | 1 cut(s) | 766 |
| AvrII | CCTAGG | 1 cut(s) | 868 |
| BaeGI | GKGCMC | 1 cut(s) | 1019 |
| BanI | GGYRCC | 1 cut(s) | 1014 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1171, 1203 |
| BbvCI | CCTCAGC | 2 cut(s) | 299, 807 |
| BbvI | GCAGC | 4 cut(s) | 301, 520, 1459, 1462 |
| BccI | CCATC | 1 cut(s) | 706 |
| BceAI | ACGGC | 1 cut(s) | 1070 |
| BclI | TGATCA | 2 cut(s) | 349, 667 |
| BcnI | CCSGG | 1 cut(s) | 388 |
| BfaI | CTAG | 6 cut(s) | 138, 363, 395, 461, 591, 869 |
| BfmI | CTRYAG | 1 cut(s) | 712 |
| BglII | AGATCT | 1 cut(s) | 159 |
| BisI | GCNGC | 6 cut(s) | 243, 315, 534, 1428, 1473, 1476 |
| BlnI | CCTAGG | 1 cut(s) | 868 |
| BlsI | GCNGC | 6 cut(s) | 244, 316, 535, 1429, 1474, 1477 |
| BmcAI | AGTACT | 1 cut(s) | 1070 |
| Bme1390I | CCNGG | 1 cut(s) | 388 |
| Bme18I | GGWCC | 1 cut(s) | 766 |
| BmgT120I | GGNCC | 4 cut(s) | 608, 766, 976, 1056 |
| BmiI | GGNNCC | 4 cut(s) | 767, 768, 1016, 1207 |
| BmrFI | CCNGG | 1 cut(s) | 388 |
| BmrI | ACTGGG | 1 cut(s) | 1091 |
| BmsI | GCATC | 2 cut(s) | 499, 1251 |
| BmuI | ACTGGG | 1 cut(s) | 1091 |
| BpmI | CTGGAG | 1 cut(s) | 213 |
| Bpu10I | CCTNAGC | 2 cut(s) | 299, 807 |
| BpuEI | CTTGAG | 1 cut(s) | 440 |
| BpuMI | CCSGG | 1 cut(s) | 388 |
| BsaBI | GATNNNNATC | 3 cut(s) | 164, 666, 697 |
| BsaJI | CCNNGG | 2 cut(s) | 868, 1287 |
| BsaWI | WCCGGW | 1 cut(s) | 65 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 463, 874 |
| Bse1I | ACTGG | 4 cut(s) | 515, 539, 1086, 1408 |
| Bse3DI | GCAATG | 1 cut(s) | 85 |
| Bse8I | GATNNNNATC | 3 cut(s) | 164, 666, 697 |
| BseDI | CCNNGG | 2 cut(s) | 868, 1287 |
| BseGI | GGATG | 2 cut(s) | 633, 1096 |
| BseJI | GATNNNNATC | 3 cut(s) | 164, 666, 697 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 463, 874 |
| BseMI | GCAATG | 1 cut(s) | 85 |
| BseMII | CTCAG | 5 cut(s) | 237, 313, 566, 821, 1056 |
| BseNI | ACTGG | 4 cut(s) | 515, 539, 1086, 1408 |
| BseRI | GAGGAG | 4 cut(s) | 33, 479, 911, 1058 |
| BseSI | GKGCMC | 1 cut(s) | 1019 |
| BseX3I | CGGCCG | 1 cut(s) | 1425 |
| BseXI | GCAGC | 4 cut(s) | 301, 520, 1459, 1462 |
| BseYI | CCCAGC | 2 cut(s) | 610, 614 |
| BsgI | GTGCAG | 1 cut(s) | 519 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1428 |
| BshFI | GGCC | 8 cut(s) | 297, 609, 805, 978, 1057, 1131, 1145, 1427 |
| BshNI | GGYRCC | 1 cut(s) | 1014 |
| BsiEI | CGRYCG | 1 cut(s) | 1428 |
| BsiSI | CCGG | 2 cut(s) | 66, 388 |
| BslFI | GGGAC | 1 cut(s) | 752 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 463, 874 |
| BsmFI | GGGAC | 1 cut(s) | 752 |
| BsnI | GGCC | 8 cut(s) | 297, 609, 805, 978, 1057, 1131, 1145, 1427 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1019 |
| Bsp143I | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| Bsp19I | CCATGG | 1 cut(s) | 1287 |
| BspACI | CCGC | 5 cut(s) | 185, 242, 816, 907, 1428 |
| BspANI | GGCC | 8 cut(s) | 297, 609, 805, 978, 1057, 1131, 1145, 1427 |
| BspCNI | CTCAG | 5 cut(s) | 238, 312, 565, 820, 1057 |
| BspLI | GGNNCC | 4 cut(s) | 767, 768, 1016, 1207 |
| BspPI | GGATC | 3 cut(s) | 433, 669, 1484 |
| BspT107I | GGYRCC | 1 cut(s) | 1014 |
| BsrDI | GCAATG | 1 cut(s) | 85 |
| BsrI | ACTGG | 4 cut(s) | 515, 539, 1086, 1408 |
| BssECI | CCNNGG | 2 cut(s) | 868, 1287 |
| BssMI | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| BssT1I | CCWWGG | 2 cut(s) | 868, 1287 |
| Bst4CI | ACNGT | 5 cut(s) | 716, 742, 955, 1037, 1123 |
| Bst6I | CTCTTC | 1 cut(s) | 981 |
| BstC8I | GCNNGC | 2 cut(s) | 531, 688 |
| BstDEI | CTNAG | 8 cut(s) | 246, 299, 552, 807, 859, 1065, 1106, 1133 |
| BstDSI | CCRYGG | 1 cut(s) | 1287 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 461 |
| BstF5I | GGATG | 2 cut(s) | 633, 1096 |
| BstHHI | GCGC | 1 cut(s) | 17 |
| BstKTI | GATC | 9 cut(s) | 162, 352, 441, 664, 670, 760, 993, 1115, 1492 |
| BstMBI | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| BstMCI | CGRYCG | 1 cut(s) | 1428 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 14, 35, 182, 239 |
| BstSCI | CCNGG | 1 cut(s) | 386 |
| BstSFI | CTRYAG | 1 cut(s) | 712 |
| BstSLI | GKGCMC | 1 cut(s) | 1019 |
| BstV1I | GCAGC | 4 cut(s) | 301, 520, 1459, 1462 |
| BstX2I | RGATCY | 2 cut(s) | 159, 661 |
| BstYI | RGATCY | 2 cut(s) | 159, 661 |
| BstZI | CGGCCG | 1 cut(s) | 1425 |
| BsuRI | GGCC | 8 cut(s) | 297, 609, 805, 978, 1057, 1131, 1145, 1427 |
| BtgI | CCRYGG | 1 cut(s) | 1287 |
| BtsCI | GGATG | 2 cut(s) | 633, 1096 |
| BtsIMutI | CAGTG | 3 cut(s) | 119, 1042, 1079 |
| Cac8I | GCNNGC | 2 cut(s) | 531, 688 |
| CfoI | GCGC | 1 cut(s) | 17 |
| Cfr13I | GGNCC | 4 cut(s) | 608, 766, 976, 1056 |
| Csp6I | GTAC | 1 cut(s) | 1069 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1108, 1143 |
| CviAII | CATG | 4 cut(s) | 470, 850, 1210, 1288 |
| CviQI | GTAC | 1 cut(s) | 1069 |
| DdeI | CTNAG | 8 cut(s) | 246, 299, 552, 807, 859, 1065, 1106, 1133 |
| DpnI | GATC | 9 cut(s) | 161, 351, 440, 663, 669, 759, 992, 1114, 1491 |
| DpnII | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| EaeI | YGGCCR | 1 cut(s) | 1425 |
| EagI | CGGCCG | 1 cut(s) | 1425 |
| Eam1104I | CTCTTC | 1 cut(s) | 981 |
| EarI | CTCTTC | 1 cut(s) | 981 |
| EclXI | CGGCCG | 1 cut(s) | 1425 |
| Eco130I | CCWWGG | 2 cut(s) | 868, 1287 |
| Eco147I | AGGCCT | 2 cut(s) | 297, 805 |
| Eco47I | GGWCC | 1 cut(s) | 766 |
| Eco52I | CGGCCG | 1 cut(s) | 1425 |
| Eco57I | CTGAAG | 1 cut(s) | 143 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 461 |
| EcoO109I | RGGNCCY | 1 cut(s) | 766 |
| EcoT14I | CCWWGG | 2 cut(s) | 868, 1287 |
| ErhI | CCWWGG | 2 cut(s) | 868, 1287 |
| FaeI | CATG | 4 cut(s) | 473, 853, 1213, 1291 |
| FaqI | GGGAC | 1 cut(s) | 752 |
| FatI | CATG | 4 cut(s) | 469, 849, 1209, 1287 |
| FbaI | TGATCA | 2 cut(s) | 349, 667 |
| Fnu4HI | GCNGC | 6 cut(s) | 243, 315, 534, 1428, 1473, 1476 |
| FokI | GGATG | 2 cut(s) | 620, 1103 |
| Fsp4HI | GCNGC | 6 cut(s) | 243, 315, 534, 1428, 1473, 1476 |
| FspBI | CTAG | 6 cut(s) | 138, 363, 395, 461, 591, 869 |
| GlaI | GCGC | 1 cut(s) | 16 |
| GluI | GCNGC | 6 cut(s) | 243, 315, 534, 1428, 1473, 1476 |
| GsaI | CCCAGC | 2 cut(s) | 614, 618 |
| GsuI | CTGGAG | 1 cut(s) | 213 |
| HaeIII | GGCC | 8 cut(s) | 297, 609, 805, 978, 1057, 1131, 1145, 1427 |
| HapII | CCGG | 2 cut(s) | 66, 388 |
| HhaI | GCGC | 1 cut(s) | 17 |
| Hin1II | CATG | 4 cut(s) | 473, 853, 1213, 1291 |
| Hin6I | GCGC | 1 cut(s) | 15 |
| HinP1I | GCGC | 1 cut(s) | 15 |
| HincII | GTYRAC | 2 cut(s) | 105, 565 |
| HindII | GTYRAC | 2 cut(s) | 105, 565 |
| HindIII | AAGCTT | 3 cut(s) | 6, 94, 499 |
| HinfI | GANTC | 5 cut(s) | 89, 188, 452, 819, 855 |
| HpaII | CCGG | 2 cut(s) | 66, 388 |
| HphI | GGTGA | 3 cut(s) | 344, 955, 1496 |
| Hpy166II | GTNNAC | 3 cut(s) | 105, 565, 877 |
| Hpy188I | TCNGA | 5 cut(s) | 337, 438, 778, 985, 1306 |
| Hpy188III | TCNNGA | 7 cut(s) | 283, 419, 461, 506, 591, 665, 1493 |
| Hpy8I | GTNNAC | 3 cut(s) | 105, 565, 877 |
| HpyAV | CCTTC | 6 cut(s) | 63, 194, 467, 934, 1173, 1372 |
| HpyCH4III | ACNGT | 5 cut(s) | 716, 742, 955, 1037, 1123 |
| HpyCH4IV | ACGT | 2 cut(s) | 107, 1503 |
| HpyCH4V | TGCA | 6 cut(s) | 49, 78, 512, 536, 686, 1370 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 14, 35, 182, 239 |
| HpyF3I | CTNAG | 8 cut(s) | 246, 299, 552, 807, 859, 1065, 1106, 1133 |
| HpySE526I | ACGT | 2 cut(s) | 107, 1503 |
| Hsp92II | CATG | 4 cut(s) | 473, 853, 1213, 1291 |
| HspAI | GCGC | 1 cut(s) | 15 |
| KflI | GGGWCCC | 1 cut(s) | 766 |
| Ksp22I | TGATCA | 2 cut(s) | 349, 667 |
| Kzo9I | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| LmnI | GCTCC | 4 cut(s) | 406, 466, 627, 1226 |
| Lsp1109I | GCAGC | 4 cut(s) | 301, 520, 1459, 1462 |
| LweI | GCATC | 2 cut(s) | 499, 1251 |
| MaeI | CTAG | 6 cut(s) | 138, 363, 395, 461, 591, 869 |
| MaeII | ACGT | 2 cut(s) | 107, 1503 |
| MaeIII | GTNAC | 5 cut(s) | 1031, 1050, 1159, 1395, 1484 |
| MalI | GATC | 9 cut(s) | 161, 351, 440, 663, 669, 759, 992, 1114, 1491 |
| MboI | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| MboII | GAAGA | 4 cut(s) | 266, 298, 998, 1094 |
| MfeI | CAATTG | 1 cut(s) | 309 |
| MflI | RGATCY | 2 cut(s) | 159, 661 |
| MhlI | GDGCHC | 1 cut(s) | 1019 |
| MluCI | AATT | 5 cut(s) | 309, 474, 1200, 1316, 1458 |
| MlyI | GAGTC | 1 cut(s) | 182 |
| MroXI | GAANNNNTTC | 3 cut(s) | 8, 456, 501 |
| MseI | TTAA | 8 cut(s) | 215, 519, 546, 569, 798, 1314, 1356, 1449 |
| MspA1I | CMGCKG | 4 cut(s) | 533, 614, 811, 1064 |
| MspI | CCGG | 2 cut(s) | 66, 388 |
| MspR9I | CCNGG | 1 cut(s) | 388 |
| MunI | CAATTG | 1 cut(s) | 309 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 14, 35, 182, 239 |
| NciI | CCSGG | 1 cut(s) | 388 |
| NcoI | CCATGG | 1 cut(s) | 1287 |
| NdeII | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| NlaIII | CATG | 4 cut(s) | 473, 853, 1213, 1291 |
| NlaIV | GGNNCC | 4 cut(s) | 767, 768, 1016, 1207 |
| NmuCI | GTSAC | 2 cut(s) | 1159, 1484 |
| PceI | AGGCCT | 2 cut(s) | 297, 805 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1016 |
| PdmI | GAANNNNTTC | 3 cut(s) | 8, 456, 501 |
| PfeI | GAWTC | 4 cut(s) | 89, 452, 819, 855 |
| PkrI | GCNGC | 6 cut(s) | 244, 316, 535, 1429, 1474, 1477 |
| PleI | GAGTC | 1 cut(s) | 182 |
| PpsI | GAGTC | 1 cut(s) | 182 |
| PpuMI | RGGWCCY | 1 cut(s) | 766 |
| PsiI | TTATAA | 1 cut(s) | 1028 |
| Psp5II | RGGWCCY | 1 cut(s) | 766 |
| PspFI | CCCAGC | 2 cut(s) | 610, 614 |
| PspN4I | GGNNCC | 4 cut(s) | 767, 768, 1016, 1207 |
| PspPI | GGNCC | 4 cut(s) | 608, 766, 976, 1056 |
| PspPPI | RGGWCCY | 1 cut(s) | 766 |
| PsuI | RGATCY | 2 cut(s) | 159, 661 |
| PvuII | CAGCTG | 4 cut(s) | 533, 614, 811, 1064 |
| RsaI | GTAC | 1 cut(s) | 1070 |
| RsaNI | GTAC | 1 cut(s) | 1069 |
| SaqAI | TTAA | 8 cut(s) | 215, 519, 546, 569, 798, 1314, 1356, 1449 |
| SatI | GCNGC | 6 cut(s) | 243, 315, 534, 1428, 1473, 1476 |
| Sau3AI | GATC | 9 cut(s) | 159, 349, 438, 661, 667, 757, 990, 1112, 1489 |
| Sau96I | GGNCC | 4 cut(s) | 608, 766, 976, 1056 |
| ScaI | AGTACT | 1 cut(s) | 1070 |
| SchI | GAGTC | 1 cut(s) | 182 |
| ScrFI | CCNGG | 1 cut(s) | 388 |
| SduI | GDGCHC | 1 cut(s) | 1019 |
| SfaNI | GCATC | 2 cut(s) | 499, 1251 |
| SfcI | CTRYAG | 1 cut(s) | 712 |
| SinI | GGWCC | 1 cut(s) | 766 |
| SmlI | CTYRAG | 1 cut(s) | 419 |
| SmoI | CTYRAG | 1 cut(s) | 419 |
| Sse9I | AATT | 5 cut(s) | 309, 474, 1200, 1316, 1458 |
| SseBI | AGGCCT | 2 cut(s) | 297, 805 |
| SsiI | CCGC | 5 cut(s) | 185, 242, 816, 907, 1428 |
| SspI | AATATT | 1 cut(s) | 838 |
| SspMI | CTAG | 6 cut(s) | 138, 363, 395, 461, 591, 869 |
| StuI | AGGCCT | 2 cut(s) | 297, 805 |
| StyD4I | CCNGG | 1 cut(s) | 386 |
| StyI | CCWWGG | 2 cut(s) | 868, 1287 |
| TaaI | ACNGT | 5 cut(s) | 716, 742, 955, 1037, 1123 |
| TaiI | ACGT | 2 cut(s) | 110, 1506 |
| TaqI | TCGA | 3 cut(s) | 744, 1041, 1111 |
| TasI | AATT | 5 cut(s) | 309, 474, 1200, 1316, 1458 |
| TatI | WGTACW | 1 cut(s) | 1068 |
| TauI | GCSGC | 2 cut(s) | 245, 1430 |
| TfiI | GAWTC | 4 cut(s) | 89, 452, 819, 855 |
| Tru1I | TTAA | 8 cut(s) | 215, 519, 546, 569, 798, 1314, 1356, 1449 |
| Tru9I | TTAA | 8 cut(s) | 215, 519, 546, 569, 798, 1314, 1356, 1449 |
| TscAI | CASTG | 3 cut(s) | 126, 1042, 1086 |
| TseFI | GTSAC | 2 cut(s) | 1159, 1484 |
| TseI | GCWGC | 4 cut(s) | 314, 533, 1472, 1475 |
| Tsp45I | GTSAC | 2 cut(s) | 1159, 1484 |
| TspDTI | ATGAA | 7 cut(s) | 17, 334, 359, 368, 846, 984, 1391 |
| TspRI | CASTG | 3 cut(s) | 126, 1042, 1086 |
| VpaK11BI | GGWCC | 1 cut(s) | 766 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 461 |
| XapI | RAATTY | 2 cut(s) | 474, 1316 |
| XbaI | TCTAGA | 2 cut(s) | 460, 590 |
| XmaJI | CCTAGG | 1 cut(s) | 868 |
| XmnI | GAANNNNTTC | 3 cut(s) | 8, 456, 501 |
| XspI | CTAG | 6 cut(s) | 138, 363, 395, 461, 591, 869 |
| ZrmI | AGTACT | 1 cut(s) | 1070 |