Belongs to the cysteine synthase cystathionine beta- synthase family
| Domain Name | Pfam ID | Position | E-value | Description |
|---|---|---|---|---|
| DAO | PF01266 | 15 - 49 | 4.4e-08 | FAD dependent oxidoreductase |
| FAD_binding_2 | PF00890 | 16 - 62 | 3.3e-06 | FAD binding domain |
| NAD_binding_8 | PF13450 | 18 - 82 | 8e-15 | NAD(P)-binding Rossmann-like domain |
| Amino_oxidase | PF01593 | 23 - 489 | 4.5e-80 | Flavin containing amine oxidoreductase |
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 1385 |
| AccBSI | CCGCTC | 1 cut(s) | 1265 |
| AccII | CGCG | 1 cut(s) | 696 |
| AccIII | TCCGGA | 1 cut(s) | 568 |
| AciI | CCGC | 5 cut(s) | 83, 131, 696, 995, 1265 |
| AclI | AACGTT | 1 cut(s) | 1032 |
| AclWI | GGATC | 3 cut(s) | 500, 530, 1519 |
| AcuI | CTGAAG | 2 cut(s) | 1470, 1487 |
| AdeI | CACNNNGTG | 1 cut(s) | 1148 |
| AfaI | GTAC | 2 cut(s) | 123, 1108 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 17, 1049, 1176, 1322 |
| AgsI | TTSAA | 6 cut(s) | 97, 230, 1021, 1212, 1225, 1349 |
| AjnI | CCWGG | 3 cut(s) | 16, 638, 1378 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1044, 1050, 1076, 1082 |
| Alw21I | GWGCWC | 1 cut(s) | 1144 |
| Alw26I | GTCTC | 2 cut(s) | 430, 550 |
| Alw44I | GTGCAC | 1 cut(s) | 1140 |
| AlwI | GGATC | 3 cut(s) | 500, 530, 1519 |
| Ama87I | CYCGRG | 1 cut(s) | 940 |
| Aor13HI | TCCGGA | 1 cut(s) | 568 |
| AoxI | GGCC | 3 cut(s) | 3, 84, 1040 |
| ApaLI | GTGCAC | 1 cut(s) | 1140 |
| ApeKI | GCWGC | 4 cut(s) | 80, 265, 322, 1451 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 776, 808 |
| AspLEI | GCGC | 2 cut(s) | 62, 696 |
| AsuC2I | CCSGG | 2 cut(s) | 520, 1441 |
| AsuHPI | GGTGA | 1 cut(s) | 994 |
| AvaI | CYCGRG | 1 cut(s) | 940 |
| AxyI | CCTNAGG | 1 cut(s) | 978 |
| BaeGI | GKGCMC | 1 cut(s) | 1144 |
| Bbv12I | GWGCWC | 1 cut(s) | 1144 |
| BbvCI | CCTCAGC | 1 cut(s) | 222 |
| BbvI | GCAGC | 4 cut(s) | 67, 277, 309, 1463 |
| BccI | CCATC | 3 cut(s) | 806, 962, 1484 |
| BceAI | ACGGC | 1 cut(s) | 921 |
| BciT130I | CCWGG | 3 cut(s) | 18, 640, 1380 |
| BclI | TGATCA | 2 cut(s) | 348, 433 |
| BcnI | CCSGG | 2 cut(s) | 520, 1441 |
| BcoDI | GTCTC | 2 cut(s) | 430, 550 |
| BfaI | CTAG | 2 cut(s) | 1103, 1146 |
| BfmI | CTRYAG | 2 cut(s) | 672, 916 |
| BfoI | RGCGCY | 1 cut(s) | 63 |
| BfuAI | ACCTGC | 1 cut(s) | 1385 |
| BglII | AGATCT | 1 cut(s) | 936 |
| BisI | GCNGC | 6 cut(s) | 81, 84, 266, 323, 995, 1452 |
| BlsI | GCNGC | 6 cut(s) | 82, 85, 267, 324, 996, 1453 |
| Bme1390I | CCNGG | 5 cut(s) | 18, 520, 640, 1380, 1441 |
| BmeT110I | CYCGRG | 1 cut(s) | 940 |
| BmiI | GGNNCC | 3 cut(s) | 399, 909, 952 |
| BmrFI | CCNGG | 5 cut(s) | 18, 520, 640, 1380, 1441 |
| BmsI | GCATC | 2 cut(s) | 880, 1488 |
| BpmI | CTGGAG | 3 cut(s) | 165, 416, 552 |
| Bpu10I | CCTNAGC | 1 cut(s) | 222 |
| BpuEI | CTTGAG | 1 cut(s) | 1067 |
| BpuMI | CCSGG | 2 cut(s) | 520, 1441 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1516 |
| BsaJI | CCNNGG | 1 cut(s) | 16 |
| BsaWI | WCCGGW | 1 cut(s) | 568 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1050, 1080 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 17, 1049, 1176, 1322 |
| Bse1I | ACTGG | 1 cut(s) | 1203 |
| Bse21I | CCTNAGG | 1 cut(s) | 978 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1516 |
| BseAI | TCCGGA | 1 cut(s) | 568 |
| BseBI | CCWGG | 3 cut(s) | 18, 640, 1380 |
| BseDI | CCNNGG | 1 cut(s) | 16 |
| BseGI | GGATG | 2 cut(s) | 193, 1222 |
| BseJI | GATNNNNATC | 1 cut(s) | 1516 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 17, 1049, 1176, 1322 |
| BseMII | CTCAG | 3 cut(s) | 201, 213, 969 |
| BseNI | ACTGG | 1 cut(s) | 1203 |
| BseSI | GKGCMC | 1 cut(s) | 1144 |
| BseXI | GCAGC | 4 cut(s) | 67, 277, 309, 1463 |
| BseYI | CCCAGC | 1 cut(s) | 611 |
| Bsh1236I | CGCG | 1 cut(s) | 696 |
| BshFI | GGCC | 3 cut(s) | 5, 86, 1042 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1144 |
| BsiHKCI | CYCGRG | 1 cut(s) | 940 |
| BsiSI | CCGG | 3 cut(s) | 519, 569, 1440 |
| BslFI | GGGAC | 1 cut(s) | 535 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 17, 1049, 1176, 1322 |
| BsmAI | GTCTC | 2 cut(s) | 430, 550 |
| BsmFI | GGGAC | 1 cut(s) | 535 |
| BsmI | GAATGC | 1 cut(s) | 868 |
| BsnI | GGCC | 3 cut(s) | 5, 86, 1042 |
| BsoBI | CYCGRG | 1 cut(s) | 940 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1144 |
| Bsp13I | TCCGGA | 1 cut(s) | 568 |
| Bsp143I | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| BspACI | CCGC | 5 cut(s) | 83, 131, 696, 995, 1265 |
| BspANI | GGCC | 3 cut(s) | 5, 86, 1042 |
| BspCNI | CTCAG | 3 cut(s) | 202, 214, 970 |
| BspEI | TCCGGA | 1 cut(s) | 568 |
| BspFNI | CGCG | 1 cut(s) | 696 |
| BspHI | TCATGA | 1 cut(s) | 808 |
| BspLI | GGNNCC | 3 cut(s) | 399, 909, 952 |
| BspMAI | CTGCAG | 1 cut(s) | 920 |
| BspMI | ACCTGC | 1 cut(s) | 1385 |
| BspPI | GGATC | 3 cut(s) | 500, 530, 1519 |
| BspQI | GCTCTTC | 1 cut(s) | 133 |
| BsrBI | CCGCTC | 1 cut(s) | 1265 |
| BsrI | ACTGG | 1 cut(s) | 1203 |
| BssECI | CCNNGG | 1 cut(s) | 16 |
| BssMI | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| Bst2UI | CCWGG | 3 cut(s) | 18, 640, 1380 |
| Bst4CI | ACNGT | 4 cut(s) | 121, 294, 806, 1416 |
| Bst6I | CTCTTC | 2 cut(s) | 133, 940 |
| BstC8I | GCNNGC | 2 cut(s) | 78, 1274 |
| BstDEI | CTNAG | 4 cut(s) | 210, 222, 978, 1521 |
| BstEII | GGTNACC | 1 cut(s) | 1397 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 15 |
| BstF5I | GGATG | 2 cut(s) | 193, 1222 |
| BstFNI | CGCG | 1 cut(s) | 696 |
| BstH2I | RGCGCY | 1 cut(s) | 63 |
| BstHHI | GCGC | 2 cut(s) | 62, 696 |
| BstKTI | GATC | 7 cut(s) | 351, 436, 508, 538, 939, 1138, 1514 |
| BstMAI | GTCTC | 2 cut(s) | 430, 550 |
| BstMBI | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 322 |
| BstNI | CCWGG | 3 cut(s) | 18, 640, 1380 |
| BstPI | GGTNACC | 1 cut(s) | 1397 |
| BstSCI | CCNGG | 5 cut(s) | 16, 518, 638, 1378, 1439 |
| BstSFI | CTRYAG | 2 cut(s) | 672, 916 |
| BstSLI | GKGCMC | 1 cut(s) | 1144 |
| BstUI | CGCG | 1 cut(s) | 696 |
| BstV1I | GCAGC | 4 cut(s) | 67, 277, 309, 1463 |
| BstX2I | RGATCY | 2 cut(s) | 535, 936 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 408, 1409 |
| BstYI | RGATCY | 2 cut(s) | 535, 936 |
| Bsu36I | CCTNAGG | 1 cut(s) | 978 |
| BsuRI | GGCC | 3 cut(s) | 5, 86, 1042 |
| BtsCI | GGATG | 2 cut(s) | 193, 1222 |
| BtsIMutI | CAGTG | 2 cut(s) | 732, 1008 |
| BveI | ACCTGC | 1 cut(s) | 1385 |
| Cac8I | GCNNGC | 2 cut(s) | 78, 1274 |
| CciI | TCATGA | 1 cut(s) | 808 |
| CfoI | GCGC | 2 cut(s) | 62, 696 |
| CseI | GACGC | 2 cut(s) | 458, 677 |
| Csp6I | GTAC | 2 cut(s) | 122, 1107 |
| CviAII | CATG | 4 cut(s) | 104, 764, 809, 1123 |
| CviQI | GTAC | 2 cut(s) | 122, 1107 |
| DdeI | CTNAG | 4 cut(s) | 210, 222, 978, 1521 |
| DpnI | GATC | 7 cut(s) | 350, 435, 507, 537, 938, 1137, 1513 |
| DpnII | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| DraIII | CACNNNGTG | 1 cut(s) | 1148 |
| Eam1104I | CTCTTC | 2 cut(s) | 133, 940 |
| EarI | CTCTTC | 2 cut(s) | 133, 940 |
| Eco147I | AGGCCT | 1 cut(s) | 1042 |
| Eco57I | CTGAAG | 2 cut(s) | 1470, 1487 |
| Eco81I | CCTNAGG | 1 cut(s) | 978 |
| Eco88I | CYCGRG | 1 cut(s) | 940 |
| Eco91I | GGTNACC | 1 cut(s) | 1397 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 15 |
| EcoO65I | GGTNACC | 1 cut(s) | 1397 |
| EcoRII | CCWGG | 3 cut(s) | 16, 638, 1378 |
| FaeI | CATG | 4 cut(s) | 107, 767, 812, 1126 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 344, 376 |
| FaqI | GGGAC | 1 cut(s) | 535 |
| FatI | CATG | 4 cut(s) | 103, 763, 808, 1122 |
| FbaI | TGATCA | 2 cut(s) | 348, 433 |
| Fnu4HI | GCNGC | 6 cut(s) | 81, 84, 266, 323, 995, 1452 |
| FokI | GGATG | 2 cut(s) | 200, 1229 |
| Fsp4HI | GCNGC | 6 cut(s) | 81, 84, 266, 323, 995, 1452 |
| FspBI | CTAG | 2 cut(s) | 1103, 1146 |
| GlaI | GCGC | 2 cut(s) | 61, 695 |
| GluI | GCNGC | 6 cut(s) | 81, 84, 266, 323, 995, 1452 |
| GsaI | CCCAGC | 1 cut(s) | 615 |
| GsuI | CTGGAG | 3 cut(s) | 165, 416, 552 |
| HaeII | RGCGCY | 1 cut(s) | 63 |
| HaeIII | GGCC | 3 cut(s) | 5, 86, 1042 |
| HapII | CCGG | 3 cut(s) | 519, 569, 1440 |
| HgaI | GACGC | 2 cut(s) | 458, 677 |
| HhaI | GCGC | 2 cut(s) | 62, 696 |
| Hin1II | CATG | 4 cut(s) | 107, 767, 812, 1126 |
| Hin6I | GCGC | 2 cut(s) | 60, 694 |
| HinP1I | GCGC | 2 cut(s) | 60, 694 |
| HindIII | AAGCTT | 1 cut(s) | 1206 |
| HinfI | GANTC | 5 cut(s) | 388, 423, 542, 1337, 1478 |
| HpaII | CCGG | 3 cut(s) | 519, 569, 1440 |
| HphI | GGTGA | 1 cut(s) | 994 |
| Hpy166II | GTNNAC | 4 cut(s) | 1109, 1142, 1288, 1412 |
| Hpy188I | TCNGA | 7 cut(s) | 276, 339, 387, 430, 829, 1243, 1489 |
| Hpy8I | GTNNAC | 4 cut(s) | 1109, 1142, 1288, 1412 |
| Hpy99I | CGWCG | 1 cut(s) | 116 |
| HpyAV | CCTTC | 5 cut(s) | 185, 520, 637, 1018, 1262 |
| HpyCH4III | ACNGT | 4 cut(s) | 121, 294, 806, 1416 |
| HpyCH4IV | ACGT | 2 cut(s) | 114, 1032 |
| HpyCH4V | TGCA | 7 cut(s) | 302, 724, 747, 918, 1142, 1394, 1446 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 322 |
| HpyF3I | CTNAG | 4 cut(s) | 210, 222, 978, 1521 |
| HpySE526I | ACGT | 2 cut(s) | 114, 1032 |
| Hsp92II | CATG | 4 cut(s) | 107, 767, 812, 1126 |
| HspAI | GCGC | 2 cut(s) | 60, 694 |
| Kpn2I | TCCGGA | 1 cut(s) | 568 |
| Ksp22I | TGATCA | 2 cut(s) | 348, 433 |
| Kzo9I | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| LguI | GCTCTTC | 1 cut(s) | 133 |
| LmnI | GCTCC | 5 cut(s) | 194, 397, 913, 1262, 1302 |
| Lsp1109I | GCAGC | 4 cut(s) | 67, 277, 309, 1463 |
| LweI | GCATC | 2 cut(s) | 880, 1488 |
| MaeI | CTAG | 2 cut(s) | 1103, 1146 |
| MaeII | ACGT | 2 cut(s) | 114, 1032 |
| MaeIII | GTNAC | 5 cut(s) | 115, 982, 1148, 1234, 1397 |
| MalI | GATC | 7 cut(s) | 350, 435, 507, 537, 938, 1137, 1513 |
| MbiI | CCGCTC | 1 cut(s) | 1265 |
| MboI | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| MflI | RGATCY | 2 cut(s) | 535, 936 |
| MhlI | GDGCHC | 1 cut(s) | 1144 |
| MluCI | AATT | 5 cut(s) | 269, 618, 629, 832, 1230 |
| MlyI | GAGTC | 1 cut(s) | 432 |
| MroI | TCCGGA | 1 cut(s) | 568 |
| MseI | TTAA | 3 cut(s) | 771, 1035, 1094 |
| MslI | CAYNNNNRTG | 2 cut(s) | 406, 1407 |
| MspA1I | CMGCKG | 1 cut(s) | 1454 |
| MspI | CCGG | 3 cut(s) | 519, 569, 1440 |
| MspR9I | CCNGG | 5 cut(s) | 18, 520, 640, 1380, 1441 |
| Mva1269I | GAATGC | 1 cut(s) | 868 |
| MvaI | CCWGG | 3 cut(s) | 18, 640, 1380 |
| MvnI | CGCG | 1 cut(s) | 696 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 322 |
| NciI | CCSGG | 2 cut(s) | 520, 1441 |
| NdeII | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| NlaIII | CATG | 4 cut(s) | 107, 767, 812, 1126 |
| NlaIV | GGNNCC | 3 cut(s) | 399, 909, 952 |
| NmuCI | GTSAC | 2 cut(s) | 982, 1148 |
| PaeR7I | CTCGAG | 1 cut(s) | 940 |
| PagI | TCATGA | 1 cut(s) | 808 |
| PceI | AGGCCT | 1 cut(s) | 1042 |
| PciSI | GCTCTTC | 1 cut(s) | 133 |
| PctI | GAATGC | 1 cut(s) | 868 |
| PfeI | GAWTC | 4 cut(s) | 388, 542, 1337, 1478 |
| PfoI | TCCNGGA | 1 cut(s) | 638 |
| PkrI | GCNGC | 6 cut(s) | 82, 85, 267, 324, 996, 1453 |
| PleI | GAGTC | 1 cut(s) | 431 |
| PpsI | GAGTC | 1 cut(s) | 431 |
| Psp1406I | AACGTT | 1 cut(s) | 1032 |
| Psp6I | CCWGG | 3 cut(s) | 16, 638, 1378 |
| PspEI | GGTNACC | 1 cut(s) | 1397 |
| PspFI | CCCAGC | 1 cut(s) | 611 |
| PspGI | CCWGG | 3 cut(s) | 16, 638, 1378 |
| PspN4I | GGNNCC | 3 cut(s) | 399, 909, 952 |
| PstI | CTGCAG | 1 cut(s) | 920 |
| PsuI | RGATCY | 2 cut(s) | 535, 936 |
| PvuII | CAGCTG | 1 cut(s) | 1454 |
| RsaI | GTAC | 2 cut(s) | 123, 1108 |
| RsaNI | GTAC | 2 cut(s) | 122, 1107 |
| RseI | CAYNNNNRTG | 2 cut(s) | 406, 1407 |
| SapI | GCTCTTC | 1 cut(s) | 133 |
| SaqAI | TTAA | 3 cut(s) | 771, 1035, 1094 |
| SatI | GCNGC | 6 cut(s) | 81, 84, 266, 323, 995, 1452 |
| Sau3AI | GATC | 7 cut(s) | 348, 433, 505, 535, 936, 1135, 1511 |
| SchI | GAGTC | 1 cut(s) | 432 |
| ScrFI | CCNGG | 5 cut(s) | 18, 520, 640, 1380, 1441 |
| SduI | GDGCHC | 1 cut(s) | 1144 |
| SfaNI | GCATC | 2 cut(s) | 880, 1488 |
| SfcI | CTRYAG | 2 cut(s) | 672, 916 |
| Sfr274I | CTCGAG | 1 cut(s) | 940 |
| SlaI | CTCGAG | 1 cut(s) | 940 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 406, 1407 |
| SmlI | CTYRAG | 2 cut(s) | 940, 1046 |
| SmoI | CTYRAG | 2 cut(s) | 940, 1046 |
| Sse9I | AATT | 5 cut(s) | 269, 618, 629, 832, 1230 |
| SseBI | AGGCCT | 1 cut(s) | 1042 |
| SsiI | CCGC | 5 cut(s) | 83, 131, 696, 995, 1265 |
| SspMI | CTAG | 2 cut(s) | 1103, 1146 |
| StuI | AGGCCT | 1 cut(s) | 1042 |
| StyD4I | CCNGG | 5 cut(s) | 16, 518, 638, 1378, 1439 |
| TaaI | ACNGT | 4 cut(s) | 121, 294, 806, 1416 |
| TaiI | ACGT | 2 cut(s) | 117, 1035 |
| TaqI | TCGA | 7 cut(s) | 111, 126, 256, 551, 941, 1186, 1356 |
| TasI | AATT | 5 cut(s) | 269, 618, 629, 832, 1230 |
| TauI | GCSGC | 2 cut(s) | 86, 997 |
| TfiI | GAWTC | 4 cut(s) | 388, 542, 1337, 1478 |
| Tru1I | TTAA | 3 cut(s) | 771, 1035, 1094 |
| Tru9I | TTAA | 3 cut(s) | 771, 1035, 1094 |
| TscAI | CASTG | 2 cut(s) | 739, 1008 |
| TseFI | GTSAC | 2 cut(s) | 982, 1148 |
| TseI | GCWGC | 4 cut(s) | 80, 265, 322, 1451 |
| Tsp45I | GTSAC | 2 cut(s) | 982, 1148 |
| TspDTI | ATGAA | 3 cut(s) | 204, 870, 947 |
| TspRI | CASTG | 2 cut(s) | 739, 1008 |
| VneI | GTGCAC | 1 cut(s) | 1140 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 15 |
| XhoI | CTCGAG | 1 cut(s) | 940 |
| XspI | CTAG | 2 cut(s) | 1103, 1146 |