Belongs to the cysteine synthase cystathionine beta- synthase family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 301 |
| Acc36I | ACCTGC | 2 cut(s) | 301, 1627 |
| AccB1I | GGYRCC | 2 cut(s) | 44, 491 |
| AccI | GTMKAC | 1 cut(s) | 6 |
| AciI | CCGC | 1 cut(s) | 117 |
| AclI | AACGTT | 1 cut(s) | 1476 |
| AclWI | GGATC | 6 cut(s) | 876, 1157, 1163, 1767, 2117, 2172 |
| AcoI | YGGCCR | 1 cut(s) | 114 |
| AcsI | RAATTY | 6 cut(s) | 129, 263, 485, 780, 1450, 1910 |
| AcuI | CTGAAG | 2 cut(s) | 792, 1764 |
| AcyI | GRCGYC | 2 cut(s) | 45, 401 |
| AfaI | GTAC | 6 cut(s) | 168, 540, 1599, 1654, 1749, 1976 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 184, 776, 1808 |
| AgsI | TTSAA | 7 cut(s) | 73, 218, 347, 377, 626, 1616, 1888 |
| AjnI | CCWGG | 1 cut(s) | 1020 |
| AluBI | AGCT | 9 cut(s) | 18, 79, 314, 596, 974, 1213, 1489, 1907, 2120 |
| AluI | AGCT | 9 cut(s) | 18, 79, 314, 596, 974, 1213, 1489, 1907, 2120 |
| Alw26I | GTCTC | 7 cut(s) | 95, 408, 1079, 1769, 2012, 2069, 2220 |
| AlwI | GGATC | 6 cut(s) | 876, 1157, 1163, 1767, 2117, 2172 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 104, 770 |
| Ama87I | CYCGRG | 2 cut(s) | 205, 601 |
| AoxI | GGCC | 3 cut(s) | 35, 114, 1044 |
| ApeKI | GCWGC | 4 cut(s) | 59, 311, 1210, 1327 |
| ApoI | RAATTY | 6 cut(s) | 129, 263, 485, 780, 1450, 1910 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 94, 126 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2200 |
| AspLEI | GCGC | 1 cut(s) | 47 |
| AspS9I | GGNCC | 4 cut(s) | 35, 1024, 1044, 1494 |
| AsuHPI | GGTGA | 5 cut(s) | 401, 542, 728, 875, 2095 |
| AsuII | TTCGAA | 1 cut(s) | 196 |
| AvaI | CYCGRG | 2 cut(s) | 205, 601 |
| AvaII | GGWCC | 2 cut(s) | 1024, 1494 |
| BanI | GGYRCC | 2 cut(s) | 44, 491 |
| BbsI | GAAGAC | 3 cut(s) | 92, 806, 1632 |
| BbvI | GCAGC | 4 cut(s) | 46, 323, 1222, 1314 |
| BccI | CCATC | 7 cut(s) | 143, 550, 770, 785, 889, 1184, 1934 |
| BceAI | ACGGC | 1 cut(s) | 1263 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1960, 1994 |
| BciT130I | CCWGG | 1 cut(s) | 1022 |
| BclI | TGATCA | 1 cut(s) | 475 |
| BcoDI | GTCTC | 7 cut(s) | 95, 408, 1079, 1769, 2012, 2069, 2220 |
| BfaI | CTAG | 8 cut(s) | 170, 251, 1214, 1223, 1290, 1365, 1862, 2094 |
| BfmI | CTRYAG | 3 cut(s) | 120, 294, 1620 |
| BfoI | RGCGCY | 1 cut(s) | 48 |
| BfuAI | ACCTGC | 2 cut(s) | 301, 1627 |
| BglII | AGATCT | 1 cut(s) | 816 |
| BisI | GCNGC | 5 cut(s) | 60, 117, 312, 1211, 1328 |
| BlsI | GCNGC | 5 cut(s) | 61, 118, 313, 1212, 1329 |
| BmcAI | AGTACT | 1 cut(s) | 168 |
| Bme1390I | CCNGG | 1 cut(s) | 1022 |
| Bme18I | GGWCC | 2 cut(s) | 1024, 1494 |
| BmeT110I | CYCGRG | 2 cut(s) | 205, 601 |
| BmgT120I | GGNCC | 4 cut(s) | 35, 1024, 1044, 1494 |
| BmiI | GGNNCC | 6 cut(s) | 46, 371, 493, 1045, 1461, 1496 |
| BmrFI | CCNGG | 1 cut(s) | 1022 |
| BmrI | ACTGGG | 1 cut(s) | 521 |
| BmsI | GCATC | 3 cut(s) | 343, 1207, 1351 |
| BmuI | ACTGGG | 1 cut(s) | 521 |
| BpiI | GAAGAC | 3 cut(s) | 92, 806, 1632 |
| BpmI | CTGGAG | 2 cut(s) | 738, 1521 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1490 |
| Bpu14I | TTCGAA | 1 cut(s) | 196 |
| BpuEI | CTTGAG | 4 cut(s) | 65, 627, 993, 1041 |
| BsaBI | GATNNNNATC | 2 cut(s) | 1167, 1758 |
| BsaHI | GRCGYC | 2 cut(s) | 45, 401 |
| BsaJI | CCNNGG | 2 cut(s) | 1020, 1733 |
| BsaWI | WCCGGW | 1 cut(s) | 334 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 184, 776, 1808 |
| Bse1I | ACTGG | 5 cut(s) | 527, 865, 1262, 1576, 2085 |
| Bse3DI | GCAATG | 2 cut(s) | 244, 1886 |
| Bse8I | GATNNNNATC | 2 cut(s) | 1167, 1758 |
| BseBI | CCWGG | 1 cut(s) | 1022 |
| BseDI | CCNNGG | 2 cut(s) | 1020, 1733 |
| BseGI | GGATG | 5 cut(s) | 145, 154, 1639, 1876, 2113 |
| BseJI | GATNNNNATC | 2 cut(s) | 1167, 1758 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 184, 776, 1808 |
| BseMI | GCAATG | 2 cut(s) | 244, 1886 |
| BseMII | CTCAG | 3 cut(s) | 818, 1508, 1764 |
| BseNI | ACTGG | 5 cut(s) | 527, 865, 1262, 1576, 2085 |
| BseRI | GAGGAG | 1 cut(s) | 902 |
| BseXI | GCAGC | 4 cut(s) | 46, 323, 1222, 1314 |
| BsgI | GTGCAG | 2 cut(s) | 330, 453 |
| BshFI | GGCC | 3 cut(s) | 37, 116, 1046 |
| BshNI | GGYRCC | 2 cut(s) | 44, 491 |
| BsiHKCI | CYCGRG | 2 cut(s) | 205, 601 |
| BsiSI | CCGG | 1 cut(s) | 335 |
| BslFI | GGGAC | 1 cut(s) | 1480 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 184, 776, 1808 |
| BsmAI | GTCTC | 7 cut(s) | 95, 408, 1079, 1769, 2012, 2069, 2220 |
| BsmBI | CGTCTC | 1 cut(s) | 408 |
| BsmFI | GGGAC | 1 cut(s) | 1480 |
| BsnI | GGCC | 3 cut(s) | 37, 116, 1046 |
| BsoBI | CYCGRG | 2 cut(s) | 205, 601 |
| Bsp119I | TTCGAA | 1 cut(s) | 196 |
| Bsp1407I | TGTACA | 1 cut(s) | 1597 |
| BspACI | CCGC | 1 cut(s) | 117 |
| BspANI | GGCC | 3 cut(s) | 37, 116, 1046 |
| BspCNI | CTCAG | 3 cut(s) | 817, 1509, 1765 |
| BspLI | GGNNCC | 6 cut(s) | 46, 371, 493, 1045, 1461, 1496 |
| BspMAI | CTGCAG | 2 cut(s) | 298, 1624 |
| BspMI | ACCTGC | 2 cut(s) | 301, 1627 |
| BspPI | GGATC | 6 cut(s) | 876, 1157, 1163, 1767, 2117, 2172 |
| BspQI | GCTCTTC | 3 cut(s) | 942, 1095, 1869 |
| BspT104I | TTCGAA | 1 cut(s) | 196 |
| BspT107I | GGYRCC | 2 cut(s) | 44, 491 |
| BsrDI | GCAATG | 2 cut(s) | 244, 1886 |
| BsrGI | TGTACA | 1 cut(s) | 1597 |
| BsrI | ACTGG | 5 cut(s) | 527, 865, 1262, 1576, 2085 |
| BssECI | CCNNGG | 2 cut(s) | 1020, 1733 |
| BssNI | GRCGYC | 2 cut(s) | 45, 401 |
| BssT1I | CCWWGG | 1 cut(s) | 1733 |
| Bst2UI | CCWGG | 1 cut(s) | 1022 |
| Bst4CI | ACNGT | 7 cut(s) | 68, 503, 1562, 1612, 1657, 1803, 2006 |
| Bst6I | CTCTTC | 5 cut(s) | 25, 942, 1077, 1095, 1869 |
| BstACI | GRCGYC | 2 cut(s) | 45, 401 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1679 |
| BstAUI | TGTACA | 1 cut(s) | 1597 |
| BstBI | TTCGAA | 1 cut(s) | 196 |
| BstC8I | GCNNGC | 2 cut(s) | 496, 2118 |
| BstDEI | CTNAG | 7 cut(s) | 804, 959, 967, 1110, 1490, 1517, 1773 |
| BstF5I | GGATG | 5 cut(s) | 145, 154, 1639, 1876, 2113 |
| BstH2I | RGCGCY | 1 cut(s) | 48 |
| BstHHI | GCGC | 1 cut(s) | 47 |
| BstMAI | GTCTC | 7 cut(s) | 95, 408, 1079, 1769, 2012, 2069, 2220 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 1679 |
| BstNI | CCWGG | 1 cut(s) | 1022 |
| BstSCI | CCNGG | 1 cut(s) | 1020 |
| BstSFI | CTRYAG | 3 cut(s) | 120, 294, 1620 |
| BstV1I | GCAGC | 4 cut(s) | 46, 323, 1222, 1314 |
| BstV2I | GAAGAC | 3 cut(s) | 92, 806, 1632 |
| BstX2I | RGATCY | 4 cut(s) | 816, 1162, 1168, 1759 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1966 |
| BstYI | RGATCY | 4 cut(s) | 816, 1162, 1168, 1759 |
| BsuRI | GGCC | 3 cut(s) | 37, 116, 1046 |
| BtsCI | GGATG | 5 cut(s) | 145, 154, 1639, 1876, 2113 |
| BtsI | GCAGTG | 1 cut(s) | 771 |
| BtsIMutI | CAGTG | 4 cut(s) | 391, 771, 1956, 2078 |
| BveI | ACCTGC | 2 cut(s) | 301, 1627 |
| Cac8I | GCNNGC | 2 cut(s) | 496, 2118 |
| CaiI | CAGNNNCTG | 2 cut(s) | 104, 770 |
| CfoI | GCGC | 1 cut(s) | 47 |
| Cfr13I | GGNCC | 4 cut(s) | 35, 1024, 1044, 1494 |
| CseI | GACGC | 1 cut(s) | 390 |
| Csp6I | GTAC | 6 cut(s) | 167, 539, 1598, 1653, 1748, 1975 |
| CviAII | CATG | 9 cut(s) | 479, 812, 851, 979, 1141, 1358, 1409, 1650, 2012 |
| CviQI | GTAC | 6 cut(s) | 167, 539, 1598, 1653, 1748, 1975 |
| DdeI | CTNAG | 7 cut(s) | 804, 959, 967, 1110, 1490, 1517, 1773 |
| DinI | GGCGCC | 1 cut(s) | 46 |
| DraI | TTTAAA | 1 cut(s) | 135 |
| EaeI | YGGCCR | 1 cut(s) | 114 |
| Eam1104I | CTCTTC | 5 cut(s) | 25, 942, 1077, 1095, 1869 |
| EarI | CTCTTC | 5 cut(s) | 25, 942, 1077, 1095, 1869 |
| Eco130I | CCWWGG | 1 cut(s) | 1733 |
| Eco32I | GATATC | 1 cut(s) | 1470 |
| Eco47I | GGWCC | 2 cut(s) | 1024, 1494 |
| Eco57I | CTGAAG | 2 cut(s) | 792, 1764 |
| Eco88I | CYCGRG | 2 cut(s) | 205, 601 |
| EcoO109I | RGGNCCY | 2 cut(s) | 35, 1494 |
| EcoRI | GAATTC | 1 cut(s) | 780 |
| EcoRII | CCWGG | 1 cut(s) | 1020 |
| EcoRV | GATATC | 1 cut(s) | 1470 |
| EcoT14I | CCWWGG | 1 cut(s) | 1733 |
| EgeI | GGCGCC | 1 cut(s) | 46 |
| EheI | GGCGCC | 1 cut(s) | 46 |
| ErhI | CCWWGG | 1 cut(s) | 1733 |
| Esp3I | CGTCTC | 1 cut(s) | 408 |
| FaeI | CATG | 9 cut(s) | 482, 815, 854, 982, 1144, 1361, 1412, 1653, 2015 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 22, 54 |
| FaqI | GGGAC | 1 cut(s) | 1480 |
| FatI | CATG | 9 cut(s) | 478, 811, 850, 978, 1140, 1357, 1408, 1649, 2011 |
| FauNDI | CATATG | 3 cut(s) | 50, 1075, 1186 |
| FbaI | TGATCA | 1 cut(s) | 475 |
| FblI | GTMKAC | 1 cut(s) | 6 |
| Fnu4HI | GCNGC | 5 cut(s) | 60, 117, 312, 1211, 1328 |
| FokI | GGATG | 5 cut(s) | 152, 161, 1646, 1883, 2120 |
| Fsp4HI | GCNGC | 5 cut(s) | 60, 117, 312, 1211, 1328 |
| FspBI | CTAG | 8 cut(s) | 170, 251, 1214, 1223, 1290, 1365, 1862, 2094 |
| GlaI | GCGC | 1 cut(s) | 46 |
| GluI | GCNGC | 5 cut(s) | 60, 117, 312, 1211, 1328 |
| GsuI | CTGGAG | 2 cut(s) | 738, 1521 |
| HaeII | RGCGCY | 1 cut(s) | 48 |
| HaeIII | GGCC | 3 cut(s) | 37, 116, 1046 |
| HapII | CCGG | 1 cut(s) | 335 |
| HgaI | GACGC | 1 cut(s) | 390 |
| HhaI | GCGC | 1 cut(s) | 47 |
| Hin1I | GRCGYC | 2 cut(s) | 45, 401 |
| Hin1II | CATG | 9 cut(s) | 482, 815, 854, 982, 1144, 1361, 1412, 1653, 2015 |
| Hin6I | GCGC | 1 cut(s) | 45 |
| HinP1I | GCGC | 1 cut(s) | 45 |
| HinfI | GANTC | 5 cut(s) | 751, 802, 1016, 1087, 1738 |
| HpaII | CCGG | 1 cut(s) | 335 |
| HphI | GGTGA | 5 cut(s) | 401, 542, 728, 875, 2095 |
| Hpy166II | GTNNAC | 5 cut(s) | 7, 392, 539, 881, 2017 |
| Hpy188I | TCNGA | 9 cut(s) | 466, 533, 798, 807, 927, 936, 1582, 1834, 2206 |
| Hpy8I | GTNNAC | 5 cut(s) | 7, 392, 539, 881, 2017 |
| HpyAV | CCTTC | 5 cut(s) | 852, 1779, 1839, 1866, 1913 |
| HpyCH4III | ACNGT | 7 cut(s) | 68, 503, 1562, 1612, 1657, 1803, 2006 |
| HpyCH4IV | ACGT | 1 cut(s) | 1476 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 1679 |
| HpyF3I | CTNAG | 7 cut(s) | 804, 959, 967, 1110, 1490, 1517, 1773 |
| HpySE526I | ACGT | 1 cut(s) | 1476 |
| Hsp92I | GRCGYC | 2 cut(s) | 45, 401 |
| Hsp92II | CATG | 9 cut(s) | 482, 815, 854, 982, 1144, 1361, 1412, 1653, 2015 |
| HspAI | GCGC | 1 cut(s) | 45 |
| KasI | GGCGCC | 1 cut(s) | 44 |
| Ksp22I | TGATCA | 1 cut(s) | 475 |
| LguI | GCTCTTC | 3 cut(s) | 942, 1095, 1869 |
| LmnI | GCTCC | 4 cut(s) | 369, 593, 1459, 1811 |
| Lsp1109I | GCAGC | 4 cut(s) | 46, 323, 1222, 1314 |
| LweI | GCATC | 3 cut(s) | 343, 1207, 1351 |
| MaeI | CTAG | 8 cut(s) | 170, 251, 1214, 1223, 1290, 1365, 1862, 2094 |
| MaeII | ACGT | 1 cut(s) | 1476 |
| MaeIII | GTNAC | 1 cut(s) | 1113 |
| MflI | RGATCY | 4 cut(s) | 816, 1162, 1168, 1759 |
| Mly113I | GGCGCC | 1 cut(s) | 45 |
| MlyI | GAGTC | 4 cut(s) | 745, 796, 1081, 1747 |
| MmeI | TCCRAC | 3 cut(s) | 1536, 1639, 2004 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2200 |
| MseI | TTAA | 6 cut(s) | 134, 423, 575, 705, 1526, 1926 |
| MslI | CAYNNNNRTG | 2 cut(s) | 876, 1095 |
| MspI | CCGG | 1 cut(s) | 335 |
| MspR9I | CCNGG | 1 cut(s) | 1022 |
| MvaI | CCWGG | 1 cut(s) | 1022 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 1679 |
| NarI | GGCGCC | 1 cut(s) | 45 |
| NdeI | CATATG | 3 cut(s) | 50, 1075, 1186 |
| NlaIII | CATG | 9 cut(s) | 482, 815, 854, 982, 1144, 1361, 1412, 1653, 2015 |
| NlaIV | GGNNCC | 6 cut(s) | 46, 371, 493, 1045, 1461, 1496 |
| NspV | TTCGAA | 1 cut(s) | 196 |
| PaeR7I | CTCGAG | 1 cut(s) | 205 |
| PaqCI | CACCTGC | 1 cut(s) | 301 |
| PciSI | GCTCTTC | 3 cut(s) | 942, 1095, 1869 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2200 |
| PfeI | GAWTC | 1 cut(s) | 1016 |
| PkrI | GCNGC | 5 cut(s) | 61, 118, 313, 1212, 1329 |
| PleI | GAGTC | 4 cut(s) | 745, 796, 1081, 1746 |
| PluTI | GGCGCC | 1 cut(s) | 48 |
| PpsI | GAGTC | 4 cut(s) | 745, 796, 1081, 1746 |
| PpuMI | RGGWCCY | 1 cut(s) | 1494 |
| Psp1406I | AACGTT | 1 cut(s) | 1476 |
| Psp5II | RGGWCCY | 1 cut(s) | 1494 |
| Psp6I | CCWGG | 1 cut(s) | 1020 |
| PspGI | CCWGG | 1 cut(s) | 1020 |
| PspN4I | GGNNCC | 6 cut(s) | 46, 371, 493, 1045, 1461, 1496 |
| PspPI | GGNCC | 4 cut(s) | 35, 1024, 1044, 1494 |
| PspPPI | RGGWCCY | 1 cut(s) | 1494 |
| PstI | CTGCAG | 2 cut(s) | 298, 1624 |
| PstNI | CAGNNNCTG | 2 cut(s) | 104, 770 |
| PsuI | RGATCY | 4 cut(s) | 816, 1162, 1168, 1759 |
| RsaI | GTAC | 6 cut(s) | 168, 540, 1599, 1654, 1749, 1976 |
| RsaNI | GTAC | 6 cut(s) | 167, 539, 1598, 1653, 1748, 1975 |
| RseI | CAYNNNNRTG | 2 cut(s) | 876, 1095 |
| SapI | GCTCTTC | 3 cut(s) | 942, 1095, 1869 |
| SaqAI | TTAA | 6 cut(s) | 134, 423, 575, 705, 1526, 1926 |
| SatI | GCNGC | 5 cut(s) | 60, 117, 312, 1211, 1328 |
| Sau96I | GGNCC | 4 cut(s) | 35, 1024, 1044, 1494 |
| ScaI | AGTACT | 1 cut(s) | 168 |
| SchI | GAGTC | 4 cut(s) | 745, 796, 1081, 1747 |
| ScrFI | CCNGG | 1 cut(s) | 1022 |
| SfaNI | GCATC | 3 cut(s) | 343, 1207, 1351 |
| SfcI | CTRYAG | 3 cut(s) | 120, 294, 1620 |
| SfoI | GGCGCC | 1 cut(s) | 46 |
| Sfr274I | CTCGAG | 1 cut(s) | 205 |
| SfuI | TTCGAA | 1 cut(s) | 196 |
| SinI | GGWCC | 2 cut(s) | 1024, 1494 |
| SlaI | CTCGAG | 1 cut(s) | 205 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 876, 1095 |
| SmlI | CTYRAG | 5 cut(s) | 80, 205, 642, 1008, 1056 |
| SmoI | CTYRAG | 5 cut(s) | 80, 205, 642, 1008, 1056 |
| SsiI | CCGC | 1 cut(s) | 117 |
| SspDI | GGCGCC | 1 cut(s) | 44 |
| SspI | AATATT | 1 cut(s) | 1150 |
| SspMI | CTAG | 8 cut(s) | 170, 251, 1214, 1223, 1290, 1365, 1862, 2094 |
| StyD4I | CCNGG | 1 cut(s) | 1020 |
| StyI | CCWWGG | 1 cut(s) | 1733 |
| TaaI | ACNGT | 7 cut(s) | 68, 503, 1562, 1612, 1657, 1803, 2006 |
| TaiI | ACGT | 1 cut(s) | 1479 |
| TaqI | TCGA | 5 cut(s) | 196, 206, 584, 1938, 2224 |
| TatI | WGTACW | 4 cut(s) | 166, 538, 1597, 1652 |
| TauI | GCSGC | 1 cut(s) | 119 |
| TfiI | GAWTC | 1 cut(s) | 1016 |
| Tru1I | TTAA | 6 cut(s) | 134, 423, 575, 705, 1526, 1926 |
| Tru9I | TTAA | 6 cut(s) | 134, 423, 575, 705, 1526, 1926 |
| TscAI | CASTG | 4 cut(s) | 391, 771, 1956, 2085 |
| TseI | GCWGC | 4 cut(s) | 59, 311, 1210, 1327 |
| TspRI | CASTG | 4 cut(s) | 391, 771, 1956, 2085 |
| VpaK11BI | GGWCC | 2 cut(s) | 1024, 1494 |
| XapI | RAATTY | 6 cut(s) | 129, 263, 485, 780, 1450, 1910 |
| XbaI | TCTAGA | 1 cut(s) | 1861 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 857 |
| XhoI | CTCGAG | 1 cut(s) | 205 |
| XmiI | GTMKAC | 1 cut(s) | 6 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2200 |
| XspI | CTAG | 8 cut(s) | 170, 251, 1214, 1223, 1290, 1365, 1862, 2094 |
| ZrmI | AGTACT | 1 cut(s) | 168 |