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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 20 |
| AgsI | TTSAA | 2 cut(s) | 62, 173 |
| AspA2I | CCTAGG | 1 cut(s) | 77 |
| AspS9I | GGNCC | 1 cut(s) | 124 |
| AsuHPI | GGTGA | 1 cut(s) | 176 |
| AvaII | GGWCC | 1 cut(s) | 124 |
| AvrII | CCTAGG | 1 cut(s) | 77 |
| BccI | CCATC | 1 cut(s) | 41 |
| BceAI | ACGGC | 1 cut(s) | 125 |
| BfaI | CTAG | 1 cut(s) | 78 |
| BglII | AGATCT | 1 cut(s) | 183 |
| BlnI | CCTAGG | 1 cut(s) | 77 |
| Bme18I | GGWCC | 1 cut(s) | 124 |
| BmgT120I | GGNCC | 1 cut(s) | 124 |
| BpuEI | CTTGAG | 1 cut(s) | 144 |
| BsaJI | CCNNGG | 1 cut(s) | 77 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 14, 44 |
| BseDI | CCNNGG | 1 cut(s) | 77 |
| BseGI | GGATG | 1 cut(s) | 52 |
| BseMII | CTCAG | 1 cut(s) | 111 |
| BseRI | GAGGAG | 2 cut(s) | 106, 143 |
| Bsp143I | GATC | 1 cut(s) | 183 |
| BspACI | CCGC | 1 cut(s) | 20 |
| BspCNI | CTCAG | 1 cut(s) | 112 |
| BssECI | CCNNGG | 1 cut(s) | 77 |
| BssMI | GATC | 1 cut(s) | 183 |
| BssT1I | CCWWGG | 1 cut(s) | 77 |
| Bst6I | CTCTTC | 1 cut(s) | 246 |
| BstDEI | CTNAG | 1 cut(s) | 120 |
| BstF5I | GGATG | 1 cut(s) | 52 |
| BstKTI | GATC | 1 cut(s) | 186 |
| BstMBI | GATC | 1 cut(s) | 183 |
| BstX2I | RGATCY | 1 cut(s) | 183 |
| BstYI | RGATCY | 1 cut(s) | 183 |
| BtsCI | GGATG | 1 cut(s) | 52 |
| Cfr13I | GGNCC | 1 cut(s) | 124 |
| CviJI | RGCY | 3 cut(s) | 140, 205, 228 |
| CviKI_1 | RGCY | 3 cut(s) | 140, 205, 228 |
| DdeI | CTNAG | 1 cut(s) | 120 |
| DpnI | GATC | 1 cut(s) | 185 |
| DpnII | GATC | 1 cut(s) | 183 |
| Eam1104I | CTCTTC | 1 cut(s) | 246 |
| EarI | CTCTTC | 1 cut(s) | 246 |
| Eco130I | CCWWGG | 1 cut(s) | 77 |
| Eco47I | GGWCC | 1 cut(s) | 124 |
| EcoO109I | RGGNCCY | 1 cut(s) | 124 |
| EcoT14I | CCWWGG | 1 cut(s) | 77 |
| ErhI | CCWWGG | 1 cut(s) | 77 |
| FaiI | YATR | 1 cut(s) | 237 |
| FokI | GGATG | 1 cut(s) | 59 |
| FspBI | CTAG | 1 cut(s) | 78 |
| HphI | GGTGA | 1 cut(s) | 176 |
| Hpy188I | TCNGA | 1 cut(s) | 121 |
| HpyAV | CCTTC | 1 cut(s) | 167 |
| HpyCH4V | TGCA | 1 cut(s) | 217 |
| HpyF3I | CTNAG | 1 cut(s) | 120 |
| Kzo9I | GATC | 1 cut(s) | 183 |
| LmnI | GCTCC | 1 cut(s) | 76 |
| LpnPI | CCDG | 1 cut(s) | 242 |
| MaeI | CTAG | 1 cut(s) | 78 |
| MalI | GATC | 1 cut(s) | 185 |
| MboI | GATC | 1 cut(s) | 183 |
| MboII | GAAGA | 1 cut(s) | 263 |
| MflI | RGATCY | 1 cut(s) | 183 |
| MluCI | AATT | 4 cut(s) | 15, 168, 209, 244 |
| MslI | CAYNNNNRTG | 1 cut(s) | 195 |
| NdeII | GATC | 1 cut(s) | 183 |
| PpuMI | RGGWCCY | 1 cut(s) | 124 |
| Psp5II | RGGWCCY | 1 cut(s) | 124 |
| PspPI | GGNCC | 1 cut(s) | 124 |
| PspPPI | RGGWCCY | 1 cut(s) | 124 |
| PsuI | RGATCY | 1 cut(s) | 183 |
| RseI | CAYNNNNRTG | 1 cut(s) | 195 |
| Sau3AI | GATC | 1 cut(s) | 183 |
| Sau96I | GGNCC | 1 cut(s) | 124 |
| SetI | ASST | 4 cut(s) | 79, 126, 166, 178 |
| SgeI | CNNG | 6 cut(s) | 90, 101, 167, 173, 226, 241 |
| SinI | GGWCC | 1 cut(s) | 124 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 195 |
| SmlI | CTYRAG | 1 cut(s) | 159 |
| SmoI | CTYRAG | 1 cut(s) | 159 |
| Sse9I | AATT | 4 cut(s) | 15, 168, 209, 244 |
| SsiI | CCGC | 1 cut(s) | 20 |
| SspMI | CTAG | 1 cut(s) | 78 |
| StyI | CCWWGG | 1 cut(s) | 77 |
| TaqI | TCGA | 1 cut(s) | 100 |
| TasI | AATT | 4 cut(s) | 15, 168, 209, 244 |
| VpaK11BI | GGWCC | 1 cut(s) | 124 |
| XmaJI | CCTAGG | 1 cut(s) | 77 |
| XspI | CTAG | 1 cut(s) | 78 |