pycom09g13910

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002047
Physical Location & Seq
Forward (+)
13072253 .. 13072876
624 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g13910.3

Sequence Viewer

Length: 315 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGTCGGGATCACGAATTATTACGTGAAAAATGGACGTAAAACAAGGAGAATGACAAGTTCAAAGTTTGGCCGGATCTTTCGGGGCTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCAAGAAATTCCGGCAAAACACCCACCGCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTGAACAGGGCGCGTGGGGGCGCGTGAGAGCTCCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

12.14

Weight (kDa)

11.97

Isoelectric Point (pI)

41.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
AciI CCGC 1 cut(s) 220
AclWI GGATC 2 cut(s) 92, 158
AcoI YGGCCR 1 cut(s) 145
AcsI RAATTY 1 cut(s) 200
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 222
AgsI TTSAA 2 cut(s) 67, 138
AluBI AGCT 4 cut(s) 17, 28, 49, 305
AluI AGCT 4 cut(s) 17, 28, 49, 305
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 2 cut(s) 92, 158
AoxI GGCC 1 cut(s) 145
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 200
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 5 cut(s) 261, 263, 274, 287, 297
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 196
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 178
Bsc4I CCNNNNNNNGG 1 cut(s) 222
Bse118I RCCGGY 1 cut(s) 222
Bse1I ACTGG 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 178
BseLI CCNNNNNNNGG 1 cut(s) 222
BseNI ACTGG 1 cut(s) 232
BsePI GCGCGC 1 cut(s) 259
BseXI GCAGC 1 cut(s) 281
Bsh1236I CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BshFI GGCC 1 cut(s) 147
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 5 cut(s) 148, 169, 205, 223, 237
BslI CCNNNNNNNGG 1 cut(s) 222
BsnI GGCC 1 cut(s) 147
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 2 cut(s) 84, 150
BspACI CCGC 1 cut(s) 220
BspANI GGCC 1 cut(s) 147
BspFNI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 2 cut(s) 92, 158
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 222
BsrI ACTGG 1 cut(s) 232
BssAI RCCGGY 1 cut(s) 222
BssECI CCNNGG 1 cut(s) 178
BssHII GCGCGC 1 cut(s) 259
BssMI GATC 2 cut(s) 84, 150
BssNI GRCGYC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 78
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 224, 261
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 178
BstFNI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BstHHI GCGC 5 cut(s) 261, 263, 274, 287, 297
BstKTI GATC 2 cut(s) 87, 153
BstMBI GATC 2 cut(s) 84, 150
BstMWI GCNNNNNNNGC 2 cut(s) 55, 269
BstUI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 260
BstX2I RGATCY 1 cut(s) 150
BstYI RGATCY 1 cut(s) 150
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 147
BtgI CCRYGG 1 cut(s) 178
Cac8I GCNNGC 2 cut(s) 224, 261
CfoI GCGC 5 cut(s) 261, 263, 274, 287, 297
Cfr10I RCCGGY 1 cut(s) 222
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 251, 265
Csp6I GTAC 1 cut(s) 5
CviJI RGCY 6 cut(s) 17, 28, 49, 147, 162, 305
CviKI_1 RGCY 6 cut(s) 17, 28, 49, 147, 162, 305
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
DpnI GATC 2 cut(s) 86, 152
DpnII GATC 2 cut(s) 84, 150
EaeI YGGCCR 1 cut(s) 145
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 305
Eco81I CCTNAGG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 307
Fnu4HI GCNGC 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 1 cut(s) 270
GlaI GCGC 5 cut(s) 260, 262, 273, 286, 296
GluI GCNGC 1 cut(s) 270
HaeIII GGCC 1 cut(s) 147
HapII CCGG 5 cut(s) 148, 169, 205, 223, 237
HgaI GACGC 2 cut(s) 251, 265
HhaI GCGC 5 cut(s) 261, 263, 274, 287, 297
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 5 cut(s) 259, 261, 272, 285, 295
HinP1I GCGC 5 cut(s) 259, 261, 272, 285, 295
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 174
HpaII CCGG 5 cut(s) 148, 169, 205, 223, 237
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 3 cut(s) 82, 88, 196
Hpy8I GTNNAC 1 cut(s) 278
Hpy99I CGWCG 2 cut(s) 83, 245
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 3 cut(s) 78, 99, 112
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 269
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 3 cut(s) 78, 99, 112
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 5 cut(s) 259, 261, 272, 285, 295
KpnI GGTACC 1 cut(s) 8
KroI GCCGGC 1 cut(s) 222
KroNI GCCGGC 1 cut(s) 224
Kzo9I GATC 2 cut(s) 84, 150
LmnI GCTCC 1 cut(s) 310
LpnPI CCDG 7 cut(s) 161, 182, 218, 236, 245, 250, 266
Lsp1109I GCAGC 1 cut(s) 281
MaeII ACGT 3 cut(s) 78, 99, 112
MalI GATC 2 cut(s) 86, 152
MauBI CGCGCGCG 1 cut(s) 259
MboI GATC 2 cut(s) 84, 150
MboII GAAGA 2 cut(s) 262, 265
MflI RGATCY 1 cut(s) 150
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 4 cut(s) 9, 91, 200, 310
MnlI CCTC 2 cut(s) 28, 240
MreI CGCCGGCG 1 cut(s) 222
MroNI GCCGGC 1 cut(s) 222
MspI CCGG 5 cut(s) 148, 169, 205, 223, 237
MvnI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
MwoI GCNNNNNNNGC 2 cut(s) 55, 269
NaeI GCCGGC 1 cut(s) 224
NdeII GATC 2 cut(s) 84, 150
NgoMIV GCCGGC 1 cut(s) 222
NlaIV GGNNCC 1 cut(s) 6
PauI GCGCGC 1 cut(s) 259
PdiI GCCGGC 1 cut(s) 224
PfeI GAWTC 1 cut(s) 174
PkrI GCNGC 1 cut(s) 271
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PsuI RGATCY 1 cut(s) 150
PteI GCGCGC 1 cut(s) 259
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SacI GAGCTC 1 cut(s) 307
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 2 cut(s) 84, 150
Sau96I GGNCC 1 cut(s) 37
SduI GDGCHC 1 cut(s) 307
SetI ASST 8 cut(s) 19, 30, 39, 51, 81, 102, 115, 307
SgrAI CRCCGGYG 1 cut(s) 222
SinI GGWCC 1 cut(s) 37
Sse9I AATT 4 cut(s) 9, 91, 200, 310
SsiI CCGC 1 cut(s) 220
SstI GAGCTC 1 cut(s) 307
TaiI ACGT 3 cut(s) 81, 102, 115
TasI AATT 4 cut(s) 9, 91, 200, 310
TfiI GAWTC 1 cut(s) 174
TseI GCWGC 1 cut(s) 269
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 200
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.