pycom08g12360

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0002047
Physical Location & Seq
Reverse (-)
10753953 .. 10754539
587 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g12360.1

Sequence Viewer

Length: 252 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGGACGTAGGAACACGGAGAACGATAAGTTCAAAGTTTGGCCGGAGCTGTCGGGGCTTTTTCCGGCGAAACCACGGCGAGTTAGGGGTCGAGACAGAAATTCCGGCAAAACACCATCTCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

84

Amino Acids

9.42

Weight (kDa)

11.11

Isoelectric Point (pI)

68.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 5 cut(s) 219, 233, 235, 237, 248
AcoI YGGCCR 1 cut(s) 116
AcsI RAATTY 1 cut(s) 175
AfaI GTAC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 67, 109
AluBI AGCT 4 cut(s) 17, 28, 49, 124
AluI AGCT 4 cut(s) 17, 28, 49, 124
Alw26I GTCTC 1 cut(s) 162
AoxI GGCC 1 cut(s) 116
ApeKI GCWGC 1 cut(s) 243
ApoI RAATTY 1 cut(s) 175
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 235, 237, 248
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 234
BccI CCATC 1 cut(s) 199
BceAI ACGGC 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 162
BisI GCNGC 1 cut(s) 244
BlsI GCNGC 1 cut(s) 245
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 234
BsaJI CCNNGG 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 178, 208
Bse1I ACTGG 1 cut(s) 206
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 149
BseNI ACTGG 1 cut(s) 206
BsePI GCGCGC 1 cut(s) 233
Bsh1236I CGCG 5 cut(s) 219, 233, 235, 237, 248
BshFI GGCC 1 cut(s) 118
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 5 cut(s) 119, 140, 180, 197, 211
BsmAI GTCTC 1 cut(s) 162
BsnI GGCC 1 cut(s) 118
BspANI GGCC 1 cut(s) 118
BspFNI CGCG 5 cut(s) 219, 233, 235, 237, 248
BspLI GGNNCC 1 cut(s) 6
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 206
BssECI CCNNGG 1 cut(s) 149
BssHII GCGCGC 1 cut(s) 233
BstC8I GCNNGC 1 cut(s) 235
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 149
BstFNI CGCG 5 cut(s) 219, 233, 235, 237, 248
BstHHI GCGC 3 cut(s) 235, 237, 248
BstMAI GTCTC 1 cut(s) 162
BstMWI GCNNNNNNNGC 3 cut(s) 55, 130, 243
BstUI CGCG 5 cut(s) 219, 233, 235, 237, 248
BstV2I GAAGAC 1 cut(s) 234
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 118
BtgI CCRYGG 1 cut(s) 149
Cac8I GCNNGC 1 cut(s) 235
CfoI GCGC 3 cut(s) 235, 237, 248
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 225, 239
Csp6I GTAC 1 cut(s) 5
CviJI RGCY 6 cut(s) 17, 28, 49, 118, 124, 133
CviKI_1 RGCY 6 cut(s) 17, 28, 49, 118, 124, 133
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 116
Eco47I GGWCC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 244
Fsp4HI GCNGC 1 cut(s) 244
GlaI GCGC 3 cut(s) 234, 236, 247
GluI GCNGC 1 cut(s) 244
HaeIII GGCC 1 cut(s) 118
HapII CCGG 5 cut(s) 119, 140, 180, 197, 211
HgaI GACGC 2 cut(s) 225, 239
HhaI GCGC 3 cut(s) 235, 237, 248
Hin6I GCGC 3 cut(s) 233, 235, 246
HinP1I GCGC 3 cut(s) 233, 235, 246
HindIII AAGCTT 1 cut(s) 26
HpaII CCGG 5 cut(s) 119, 140, 180, 197, 211
HphI GGTGA 1 cut(s) 44
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 1 cut(s) 167
Hpy99I CGWCG 1 cut(s) 219
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 1 cut(s) 82
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 130, 243
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 82
HspAI GCGC 3 cut(s) 233, 235, 246
KpnI GGTACC 1 cut(s) 8
LmnI GCTCC 1 cut(s) 121
LpnPI CCDG 6 cut(s) 132, 153, 193, 210, 219, 224
MaeII ACGT 1 cut(s) 82
MauBI CGCGCGCG 1 cut(s) 233
MboII GAAGA 2 cut(s) 236, 239
MluCI AATT 2 cut(s) 9, 175
MnlI CCTC 2 cut(s) 28, 214
MspI CCGG 5 cut(s) 119, 140, 180, 197, 211
MvnI CGCG 5 cut(s) 219, 233, 235, 237, 248
MwoI GCNNNNNNNGC 3 cut(s) 55, 130, 243
NlaIV GGNNCC 1 cut(s) 6
PauI GCGCGC 1 cut(s) 233
PkrI GCNGC 1 cut(s) 245
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 233
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SatI GCNGC 1 cut(s) 244
Sau96I GGNCC 1 cut(s) 37
SetI ASST 6 cut(s) 19, 30, 39, 51, 85, 126
SinI GGWCC 1 cut(s) 37
Sse9I AATT 2 cut(s) 9, 175
TaiI ACGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 166
TasI AATT 2 cut(s) 9, 175
TseI GCWGC 1 cut(s) 243
TspGWI ACGGA 2 cut(s) 93, 107
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 175
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.