pycom07g11300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0002047
Physical Location & Seq
Forward (+)
11948519 .. 11949223
705 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g11300.4

Sequence Viewer

Length: 396 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGTCGGGATCACGAACTATTACGTGAAAAATGGACGTAAGAACAAGGAGAATGACAAGTTCAAAGTTTGGCCGGAGCTTTCGGGGCTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCAAGAAATTCCGGCAAAACACCCACCGCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCACGCGTGGGCAGCGCGTGGGGGCGCGTGAACAGGGCGCGTGAGAGCTCCAAATTGAGTTCTGAAAATTGTCACGCGTTCGTGACGTCGTGTAGATCAACGTGGTATATTCATACACCCAAATTGAGCAACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

132

Amino Acids

14.95

Weight (kDa)

10.28

Isoelectric Point (pI)

53.16

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 81, 347
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
AciI CCGC 1 cut(s) 221
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 201
AcyI GRCGYC 2 cut(s) 78, 344
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 223
AflIII ACRYGT 2 cut(s) 262, 333
AgsI TTSAA 2 cut(s) 67, 139
AluBI AGCT 5 cut(s) 17, 28, 49, 154, 306
AluI AGCT 5 cut(s) 17, 28, 49, 154, 306
Alw21I GWGCWC 1 cut(s) 308
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 270
ApoI RAATTY 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 275, 285, 298
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 308
BbsI GAAGAC 1 cut(s) 261
Bbv12I GWGCWC 1 cut(s) 308
BbvI GCAGC 1 cut(s) 282
BceAI ACGGC 1 cut(s) 197
BisI GCNGC 1 cut(s) 271
BlsI GCNGC 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 261
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 78, 344
BsaJI CCNNGG 1 cut(s) 179
Bsc4I CCNNNNNNNGG 1 cut(s) 223
Bse118I RCCGGY 1 cut(s) 223
Bse1I ACTGG 1 cut(s) 233
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 179
BseLI CCNNNNNNNGG 1 cut(s) 223
BseNI ACTGG 1 cut(s) 233
BseXI GCAGC 1 cut(s) 282
Bsh1236I CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 308
BsiSI CCGG 5 cut(s) 149, 170, 206, 224, 238
BslI CCNNNNNNNGG 1 cut(s) 223
BsnI GGCC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 308
Bsp143I GATC 2 cut(s) 84, 353
BspACI CCGC 1 cut(s) 221
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 1 cut(s) 223
BsrI ACTGG 1 cut(s) 233
BssAI RCCGGY 1 cut(s) 223
BssECI CCNNGG 1 cut(s) 179
BssMI GATC 2 cut(s) 84, 353
BssNI GRCGYC 2 cut(s) 78, 344
BstACI GRCGYC 2 cut(s) 78, 344
BstBAI YACGTR 1 cut(s) 100
BstC8I GCNNGC 2 cut(s) 225, 262
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 179
BstFNI CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
BstHHI GCGC 3 cut(s) 275, 285, 298
BstKTI GATC 2 cut(s) 87, 356
BstMBI GATC 2 cut(s) 84, 353
BstMWI GCNNNNNNNGC 3 cut(s) 55, 160, 270
BstUI CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
BstV1I GCAGC 1 cut(s) 282
BstV2I GAAGAC 1 cut(s) 261
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 225, 262
CfoI GCGC 3 cut(s) 275, 285, 298
Cfr10I RCCGGY 1 cut(s) 223
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 252, 266
Csp6I GTAC 1 cut(s) 5
CviJI RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 306
CviKI_1 RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 306
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
DpnI GATC 2 cut(s) 86, 355
DpnII GATC 2 cut(s) 84, 353
EaeI YGGCCR 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 306
Eco24I GRGCYC 1 cut(s) 308
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 306
Eco81I CCTNAGG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 306
EcoO109I RGGNCCY 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 308
FaiI YATR 3 cut(s) 366, 372, 394
Fnu4HI GCNGC 1 cut(s) 271
FriOI GRGCYC 1 cut(s) 308
Fsp4HI GCNGC 1 cut(s) 271
GlaI GCGC 3 cut(s) 274, 284, 297
GluI GCNGC 1 cut(s) 271
HaeIII GGCC 1 cut(s) 148
HapII CCGG 5 cut(s) 149, 170, 206, 224, 238
HgaI GACGC 2 cut(s) 252, 266
HhaI GCGC 3 cut(s) 275, 285, 298
Hin1I GRCGYC 2 cut(s) 78, 344
Hin6I GCGC 3 cut(s) 273, 283, 296
HinP1I GCGC 3 cut(s) 273, 283, 296
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 175
HpaII CCGG 5 cut(s) 149, 170, 206, 224, 238
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 289
Hpy188I TCNGA 2 cut(s) 53, 322
Hpy188III TCNNGA 4 cut(s) 82, 88, 197, 340
Hpy8I GTNNAC 1 cut(s) 289
Hpy99I CGWCG 3 cut(s) 83, 246, 349
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 6 cut(s) 78, 99, 112, 344, 359, 390
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 160, 270
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 6 cut(s) 78, 99, 112, 344, 359, 390
Hsp92I GRCGYC 2 cut(s) 78, 344
HspAI GCGC 3 cut(s) 273, 283, 296
KpnI GGTACC 1 cut(s) 8
KroI GCCGGC 1 cut(s) 223
KroNI GCCGGC 1 cut(s) 225
Kzo9I GATC 2 cut(s) 84, 353
LmnI GCTCC 2 cut(s) 151, 311
LpnPI CCDG 7 cut(s) 162, 183, 219, 237, 246, 251, 277
Lsp1109I GCAGC 1 cut(s) 282
MaeII ACGT 6 cut(s) 78, 99, 112, 344, 359, 390
MaeIII GTNAC 2 cut(s) 329, 340
MalI GATC 2 cut(s) 86, 355
MboI GATC 2 cut(s) 84, 353
MboII GAAGA 2 cut(s) 263, 266
MhlI GDGCHC 1 cut(s) 308
MluCI AATT 5 cut(s) 9, 201, 311, 325, 380
MluI ACGCGT 2 cut(s) 262, 333
MnlI CCTC 2 cut(s) 28, 241
MreI CGCCGGCG 1 cut(s) 223
MroNI GCCGGC 1 cut(s) 223
MspI CCGG 5 cut(s) 149, 170, 206, 224, 238
MvnI CGCG 6 cut(s) 246, 264, 275, 285, 298, 335
MwoI GCNNNNNNNGC 3 cut(s) 55, 160, 270
NaeI GCCGGC 1 cut(s) 225
NdeII GATC 2 cut(s) 84, 353
NgoMIV GCCGGC 1 cut(s) 223
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 329, 340
PdiI GCCGGC 1 cut(s) 225
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 308
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SacI GAGCTC 1 cut(s) 308
SatI GCNGC 1 cut(s) 271
Sau3AI GATC 2 cut(s) 84, 353
Sau96I GGNCC 1 cut(s) 37
SduI GDGCHC 1 cut(s) 308
SgrAI CRCCGGYG 1 cut(s) 223
SinI GGWCC 1 cut(s) 37
Sse9I AATT 5 cut(s) 9, 201, 311, 325, 380
SsiI CCGC 1 cut(s) 221
SstI GAGCTC 1 cut(s) 308
TaiI ACGT 6 cut(s) 81, 102, 115, 347, 362, 393
TasI AATT 5 cut(s) 9, 201, 311, 325, 380
TfiI GAWTC 1 cut(s) 175
TseFI GTSAC 2 cut(s) 329, 340
TseI GCWGC 1 cut(s) 270
Tsp45I GTSAC 2 cut(s) 329, 340
TspDTI ATGAA 1 cut(s) 359
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 201
ZraI GACGTC 2 cut(s) 79, 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.