pycom15g30190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0002047
Physical Location & Seq
Reverse (-)
27536775 .. 27537457
683 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g30190.5

Sequence Viewer

Length: 336 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGTCGGGATCACGAACTATTACGTGAAAAATGGATGTAGGAACACGGAGAATGACAAGTTCAAAGTTTGGCCGGAGCTTTCGGGGCTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCGAGGAAGAAATTCCGGCAAAACACCCACCGTCGGCGAAACCAGTCCGAGCTCCAAATTGAGTTCTGAAAATTGTCACGCGTTCGTGACGTCGTGTAGATCACCATGGTATATTCATACACCCAAATTGAGCAACGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

112

Amino Acids

12.39

Weight (kDa)

10.03

Isoelectric Point (pI)

40.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 81, 287
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 275
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 2 cut(s) 78, 284
AfaI GTAC 1 cut(s) 6
AfiI CCNNNNNNNGG 1 cut(s) 227
AflIII ACRYGT 1 cut(s) 273
AgsI TTSAA 2 cut(s) 67, 139
AluBI AGCT 5 cut(s) 17, 28, 49, 154, 246
AluI AGCT 5 cut(s) 17, 28, 49, 154, 246
Alw21I GWGCWC 1 cut(s) 248
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 146
ApoI RAATTY 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 205
Asp718I GGTACC 1 cut(s) 4
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 2 cut(s) 44, 288
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 248
Bbv12I GWGCWC 1 cut(s) 248
BceAI ACGGC 1 cut(s) 197
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BsaAI YACGTR 1 cut(s) 100
BsaHI GRCGYC 2 cut(s) 78, 284
BsaJI CCNNGG 2 cut(s) 179, 299
Bsc4I CCNNNNNNNGG 1 cut(s) 227
Bse1I ACTGG 1 cut(s) 237
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 2 cut(s) 179, 299
BseGI GGATG 1 cut(s) 116
BseLI CCNNNNNNNGG 1 cut(s) 227
BseNI ACTGG 1 cut(s) 237
Bsh1236I CGCG 1 cut(s) 275
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 248
BsiSI CCGG 3 cut(s) 149, 170, 210
BslI CCNNNNNNNGG 1 cut(s) 227
BsnI GGCC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 248
Bsp143I GATC 2 cut(s) 84, 293
Bsp19I CCATGG 1 cut(s) 299
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 1 cut(s) 275
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 237
BssECI CCNNGG 2 cut(s) 179, 299
BssMI GATC 2 cut(s) 84, 293
BssNI GRCGYC 2 cut(s) 78, 284
BssT1I CCWWGG 1 cut(s) 299
Bst4CI ACNGT 1 cut(s) 226
BstACI GRCGYC 2 cut(s) 78, 284
BstBAI YACGTR 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 2 cut(s) 179, 299
BstF5I GGATG 1 cut(s) 116
BstFNI CGCG 1 cut(s) 275
BstKTI GATC 2 cut(s) 87, 296
BstMBI GATC 2 cut(s) 84, 293
BstMWI GCNNNNNNNGC 2 cut(s) 55, 160
BstUI CGCG 1 cut(s) 275
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 2 cut(s) 179, 299
BtsCI GGATG 1 cut(s) 116
Cfr13I GGNCC 1 cut(s) 37
Csp6I GTAC 1 cut(s) 5
CviAII CATG 1 cut(s) 300
CviJI RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 246
CviKI_1 RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 246
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
DpnI GATC 2 cut(s) 86, 295
DpnII GATC 2 cut(s) 84, 293
EaeI YGGCCR 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 246
Eco130I CCWWGG 1 cut(s) 299
Eco24I GRGCYC 1 cut(s) 248
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 246
Eco81I CCTNAGG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 246
EcoO109I RGGNCCY 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 299
EcoT38I GRGCYC 1 cut(s) 248
ErhI CCWWGG 1 cut(s) 299
FaeI CATG 1 cut(s) 303
FaiI YATR 4 cut(s) 301, 306, 312, 334
FatI CATG 1 cut(s) 299
FokI GGATG 1 cut(s) 123
FriOI GRGCYC 1 cut(s) 248
HaeIII GGCC 1 cut(s) 148
HapII CCGG 3 cut(s) 149, 170, 210
Hin1I GRCGYC 2 cut(s) 78, 284
Hin1II CATG 1 cut(s) 303
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 175
HpaII CCGG 3 cut(s) 149, 170, 210
HphI GGTGA 2 cut(s) 44, 288
Hpy188I TCNGA 3 cut(s) 53, 243, 262
Hpy188III TCNNGA 3 cut(s) 82, 88, 280
Hpy99I CGWCG 3 cut(s) 83, 230, 289
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 226
HpyCH4IV ACGT 4 cut(s) 78, 99, 284, 330
HpyF10VI GCNNNNNNNGC 2 cut(s) 55, 160
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 4 cut(s) 78, 99, 284, 330
Hsp92I GRCGYC 2 cut(s) 78, 284
Hsp92II CATG 1 cut(s) 303
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 2 cut(s) 84, 293
LmnI GCTCC 2 cut(s) 151, 251
LpnPI CCDG 4 cut(s) 162, 183, 223, 250
MaeII ACGT 4 cut(s) 78, 99, 284, 330
MaeIII GTNAC 2 cut(s) 269, 280
MalI GATC 2 cut(s) 86, 295
MboI GATC 2 cut(s) 84, 293
MboII GAAGA 1 cut(s) 213
MhlI GDGCHC 1 cut(s) 248
MluCI AATT 5 cut(s) 9, 205, 251, 265, 320
MluI ACGCGT 1 cut(s) 273
MnlI CCTC 2 cut(s) 28, 191
MroXI GAANNNNTTC 1 cut(s) 205
MspI CCGG 3 cut(s) 149, 170, 210
MvnI CGCG 1 cut(s) 275
MwoI GCNNNNNNNGC 2 cut(s) 55, 160
NcoI CCATGG 1 cut(s) 299
NdeII GATC 2 cut(s) 84, 293
NlaIII CATG 1 cut(s) 303
NlaIV GGNNCC 1 cut(s) 6
NmuCI GTSAC 2 cut(s) 269, 280
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 100
PpuMI RGGWCCY 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 248
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SacI GAGCTC 1 cut(s) 248
Sau3AI GATC 2 cut(s) 84, 293
Sau96I GGNCC 1 cut(s) 37
SduI GDGCHC 1 cut(s) 248
SinI GGWCC 1 cut(s) 37
Sse9I AATT 5 cut(s) 9, 205, 251, 265, 320
SstI GAGCTC 1 cut(s) 248
StyI CCWWGG 1 cut(s) 299
TaaI ACNGT 1 cut(s) 226
TaiI ACGT 4 cut(s) 81, 102, 287, 333
TaqI TCGA 1 cut(s) 196
TasI AATT 5 cut(s) 9, 205, 251, 265, 320
TfiI GAWTC 1 cut(s) 175
TseFI GTSAC 2 cut(s) 269, 280
Tsp45I GTSAC 2 cut(s) 269, 280
TspDTI ATGAA 1 cut(s) 299
TspGWI ACGGA 1 cut(s) 137
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 205
ZraI GACGTC 2 cut(s) 79, 285
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.