pycom02g08310

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0002047
Physical Location & Seq
Forward (+)
5792511 .. 5793850
1340 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g08310.4

Sequence Viewer

Length: 903 bp
ATGGCAACTATGATGGGACAAAAATACCTTCCAAACAAAAAAGTTCAAAAACAGCCCAAAATAATGCAAGAATATTTTCGTAATATAAACATTGTGTTTTTACAATTAATTTTTTTTATACACAATAATCTGATTCAATTATCAAAACATTTTAACAATCGCGTAATGCTGATTATAAAAATGATGTTTCGCGTGCGTATTTACACTAAAGAAAAGGGGATAGACTTATGTGACAGCCCGTCCCGAATTAACATTTTCATATGTGTGAAGTTGTGTGGAATGATTAAAGACAGGGCACTGTGCATACACGGGTTGCACGTTTCTTCTCCTTTTTCTTTTCCTTTCCGTGATTTCCTCAAACCTGTTTTTCATTCTTCCTCTCTCCCCGTCAAGCTCTCTCTCTCTCTCCCTCACTCTCAAACACACGGCAACAACACCAATTCTTCAAAACTTCACGGATCGACGAAAATAAAATCACCATCTTCTTCGTTTCGACGATACGAGTCCAACCATACCAATTTCAGGACTTTTCAAGCTTATATGAAGCTTCTTGAGGTCCTAAGGAGGCTTGAAGTAGTTCGTTGGAAGTTTTTGGACGTAAGAACACGGAGAACGACAAGTTCAAAGTTTGTCCGGAGCTTTCGGGGCTTTTTCTGGTGTTCGTCTCGTCAATACCTTCATTTTGATATAAATTTACCCAGAAATTCCGGCAAAACACCCACCTCCGGCGAAACCAGTCCGACGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTATACAGGGCGCGTGAGAGCTCCAAATTGAGTTCTAAAAGTTGTCACGTGTTCGTGACGTCGTGTAGATCGCCGTGGTTTGTGTATGTGCGTTTAAATGATGATTTTATAATTATTCTTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

301

Amino Acids

35.28

Weight (kDa)

10.92

Isoelectric Point (pI)

55.1

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 176, 887
AatII GACGTC 1 cut(s) 839
AccI GTMKAC 1 cut(s) 780
AccII CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
AccIII TCCGGA 1 cut(s) 633
AclWI GGATC 1 cut(s) 466
AcsI RAATTY 2 cut(s) 691, 703
AcvI CACGTG 1 cut(s) 826
AcyI GRCGYC 1 cut(s) 836
AfiI CCNNNNNNNGG 2 cut(s) 522, 725
AflIII ACRYGT 1 cut(s) 825
AgsI TTSAA 6 cut(s) 47, 137, 447, 533, 572, 624
AhdI GACNNNNNGTC 1 cut(s) 238
AluBI AGCT 5 cut(s) 394, 536, 547, 639, 798
AluI AGCT 5 cut(s) 394, 536, 547, 639, 798
Alw21I GWGCWC 1 cut(s) 800
Alw26I GTCTC 1 cut(s) 669
AlwI GGATC 1 cut(s) 466
Aor13HI TCCGGA 1 cut(s) 633
ApeKI GCWGC 1 cut(s) 772
ApoI RAATTY 2 cut(s) 691, 703
AseI ATTAAT 1 cut(s) 107
Asp700I GAANNNNTTC 2 cut(s) 75, 576
AspLEI GCGC 4 cut(s) 764, 766, 777, 790
AspS9I GGNCC 1 cut(s) 556
AsuHPI GGTGA 1 cut(s) 468
AvaII GGWCC 1 cut(s) 556
AxyI CCTNAGG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 298
BanII GRGCYC 1 cut(s) 800
BbrPI CACGTG 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 763
Bbv12I GWGCWC 1 cut(s) 800
BbvI GCAGC 1 cut(s) 784
BccI CCATC 2 cut(s) 7, 487
BceAI ACGGC 2 cut(s) 442, 835
BcoDI GTCTC 1 cut(s) 669
BisI GCNGC 1 cut(s) 773
BlsI GCNGC 1 cut(s) 774
Bme18I GGWCC 1 cut(s) 556
BmeRI GACNNNNNGTC 1 cut(s) 238
BmgT120I GGNCC 1 cut(s) 556
BpiI GAAGAC 1 cut(s) 763
BpuEI CTTGAG 1 cut(s) 572
BsaAI YACGTR 1 cut(s) 826
BsaHI GRCGYC 1 cut(s) 836
BsaJI CCNNGG 1 cut(s) 851
BsaWI WCCGGW 1 cut(s) 633
Bsc4I CCNNNNNNNGG 2 cut(s) 522, 725
Bse1I ACTGG 1 cut(s) 735
Bse21I CCTNAGG 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 633
BseDI CCNNGG 1 cut(s) 851
BseLI CCNNNNNNNGG 2 cut(s) 522, 725
BseNI ACTGG 1 cut(s) 735
BsePI GCGCGC 1 cut(s) 762
BseSI GKGCMC 1 cut(s) 298
BseXI GCAGC 1 cut(s) 784
Bsh1236I CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
BsiHKAI GWGCWC 1 cut(s) 800
BsiSI CCGG 3 cut(s) 634, 708, 726
BslFI GGGAC 2 cut(s) 30, 226
BslI CCNNNNNNNGG 2 cut(s) 522, 725
BsmAI GTCTC 1 cut(s) 669
BsmBI CGTCTC 1 cut(s) 669
BsmFI GGGAC 2 cut(s) 30, 226
Bsp1286I GDGCHC 2 cut(s) 298, 800
Bsp13I TCCGGA 1 cut(s) 633
Bsp143I GATC 2 cut(s) 458, 845
BspEI TCCGGA 1 cut(s) 633
BspFNI CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
BspPI GGATC 1 cut(s) 466
BsrI ACTGG 1 cut(s) 735
BssECI CCNNGG 1 cut(s) 851
BssHII GCGCGC 1 cut(s) 762
BssMI GATC 2 cut(s) 458, 845
BssNAI GTATAC 1 cut(s) 781
BssNI GRCGYC 1 cut(s) 836
Bst1107I GTATAC 1 cut(s) 781
Bst4CI ACNGT 1 cut(s) 300
BstACI GRCGYC 1 cut(s) 836
BstBAI YACGTR 1 cut(s) 826
BstC8I GCNNGC 2 cut(s) 194, 764
BstDEI CTNAG 1 cut(s) 560
BstDSI CCRYGG 1 cut(s) 851
BstFNI CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
BstHHI GCGC 4 cut(s) 764, 766, 777, 790
BstKTI GATC 2 cut(s) 461, 848
BstMAI GTCTC 1 cut(s) 669
BstMBI GATC 2 cut(s) 458, 845
BstMWI GCNNNNNNNGC 2 cut(s) 645, 772
BstSLI GKGCMC 1 cut(s) 298
BstUI CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
BstV1I GCAGC 1 cut(s) 784
BstV2I GAAGAC 1 cut(s) 763
BstZ17I GTATAC 1 cut(s) 781
Bsu36I CCTNAGG 1 cut(s) 560
BtgI CCRYGG 1 cut(s) 851
BtsIMutI CAGTG 1 cut(s) 296
Cac8I GCNNGC 2 cut(s) 194, 764
CfoI GCGC 4 cut(s) 764, 766, 777, 790
Cfr13I GGNCC 1 cut(s) 556
CseI GACGC 2 cut(s) 754, 768
CviJI RGCY 9 cut(s) 55, 237, 394, 536, 547, 568, 639, 648, 798
CviKI_1 RGCY 9 cut(s) 55, 237, 394, 536, 547, 568, 639, 648, 798
DdeI CTNAG 1 cut(s) 560
DpnI GATC 2 cut(s) 460, 847
DpnII GATC 2 cut(s) 458, 845
DraI TTTAAA 1 cut(s) 873
DriI GACNNNNNGTC 1 cut(s) 238
Eam1105I GACNNNNNGTC 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 798
Eco24I GRGCYC 1 cut(s) 800
Eco47I GGWCC 1 cut(s) 556
Eco53kI GAGCTC 1 cut(s) 798
Eco72I CACGTG 1 cut(s) 826
Eco81I CCTNAGG 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 798
EcoO109I RGGNCCY 1 cut(s) 556
EcoT38I GRGCYC 1 cut(s) 800
Esp3I CGTCTC 1 cut(s) 669
FaqI GGGAC 2 cut(s) 30, 226
FauNDI CATATG 1 cut(s) 260
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 1 cut(s) 773
FriOI GRGCYC 1 cut(s) 800
Fsp4HI GCNGC 1 cut(s) 773
GlaI GCGC 4 cut(s) 763, 765, 776, 789
GluI GCNGC 1 cut(s) 773
HapII CCGG 3 cut(s) 634, 708, 726
HgaI GACGC 2 cut(s) 754, 768
HhaI GCGC 4 cut(s) 764, 766, 777, 790
Hin1I GRCGYC 1 cut(s) 836
Hin6I GCGC 4 cut(s) 762, 764, 775, 788
HinP1I GCGC 4 cut(s) 762, 764, 775, 788
HindIII AAGCTT 2 cut(s) 534, 545
HinfI GANTC 2 cut(s) 133, 503
HpaII CCGG 3 cut(s) 634, 708, 726
HphI GGTGA 1 cut(s) 468
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 2 cut(s) 132, 741
Hpy188III TCNNGA 5 cut(s) 243, 523, 551, 634, 832
Hpy8I GTNNAC 1 cut(s) 781
Hpy99I CGWCG 5 cut(s) 466, 498, 745, 748, 841
HpyAV CCTTC 2 cut(s) 38, 686
HpyCH4III ACNGT 1 cut(s) 300
HpyCH4IV ACGT 4 cut(s) 318, 597, 825, 836
HpyCH4V TGCA 3 cut(s) 67, 303, 316
HpyF10VI GCNNNNNNNGC 2 cut(s) 645, 772
HpyF3I CTNAG 1 cut(s) 560
HpySE526I ACGT 4 cut(s) 318, 597, 825, 836
Hsp92I GRCGYC 1 cut(s) 836
HspAI GCGC 4 cut(s) 762, 764, 775, 788
Kpn2I TCCGGA 1 cut(s) 633
Kzo9I GATC 2 cut(s) 458, 845
LmnI GCTCC 2 cut(s) 636, 803
Lsp1109I GCAGC 1 cut(s) 784
MaeII ACGT 4 cut(s) 318, 597, 825, 836
MaeIII GTNAC 3 cut(s) 230, 821, 832
MalI GATC 2 cut(s) 460, 847
MauBI CGCGCGCG 1 cut(s) 762
MboI GATC 2 cut(s) 458, 845
MboII GAAGA 7 cut(s) 315, 366, 435, 474, 477, 765, 768
MhlI GDGCHC 2 cut(s) 298, 800
MlyI GAGTC 1 cut(s) 512
MmeI TCCRAC 3 cut(s) 531, 563, 764
MnlI CCTC 7 cut(s) 365, 388, 420, 547, 558, 733, 743
MroI TCCGGA 1 cut(s) 633
MroXI GAANNNNTTC 2 cut(s) 75, 576
MseI TTAA 5 cut(s) 107, 153, 249, 285, 872
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 3 cut(s) 634, 708, 726
MvnI CGCG 8 cut(s) 162, 192, 748, 762, 764, 766, 777, 790
MwoI GCNNNNNNNGC 2 cut(s) 645, 772
NdeI CATATG 1 cut(s) 260
NdeII GATC 2 cut(s) 458, 845
NmuCI GTSAC 3 cut(s) 230, 821, 832
PauI GCGCGC 1 cut(s) 762
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PdmI GAANNNNTTC 2 cut(s) 75, 576
PfeI GAWTC 1 cut(s) 133
PkrI GCNGC 1 cut(s) 774
PleI GAGTC 1 cut(s) 511
PmaCI CACGTG 1 cut(s) 826
PmlI CACGTG 1 cut(s) 826
PpsI GAGTC 1 cut(s) 511
Ppu21I YACGTR 1 cut(s) 826
PpuMI RGGWCCY 1 cut(s) 556
PshBI ATTAAT 1 cut(s) 107
PsiI TTATAA 2 cut(s) 176, 887
Psp124BI GAGCTC 1 cut(s) 800
Psp5II RGGWCCY 1 cut(s) 556
PspCI CACGTG 1 cut(s) 826
PspPI GGNCC 1 cut(s) 556
PspPPI RGGWCCY 1 cut(s) 556
PteI GCGCGC 1 cut(s) 762
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 800
SaqAI TTAA 5 cut(s) 107, 153, 249, 285, 872
SatI GCNGC 1 cut(s) 773
Sau3AI GATC 2 cut(s) 458, 845
Sau96I GGNCC 1 cut(s) 556
SchI GAGTC 1 cut(s) 512
SduI GDGCHC 2 cut(s) 298, 800
SinI GGWCC 1 cut(s) 556
SmiMI CAYNNNNRTG 1 cut(s) 263
SmlI CTYRAG 1 cut(s) 551
SmoI CTYRAG 1 cut(s) 551
SspI AATATT 1 cut(s) 74
SstI GAGCTC 1 cut(s) 800
TaaI ACNGT 1 cut(s) 300
TaiI ACGT 4 cut(s) 321, 600, 828, 839
TaqI TCGA 2 cut(s) 461, 493
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 5 cut(s) 107, 153, 249, 285, 872
Tru9I TTAA 5 cut(s) 107, 153, 249, 285, 872
TscAI CASTG 1 cut(s) 303
TseFI GTSAC 3 cut(s) 230, 821, 832
TseI GCWGC 1 cut(s) 772
Tsp45I GTSAC 3 cut(s) 230, 821, 832
TspDTI ATGAA 4 cut(s) 247, 359, 557, 668
TspGWI ACGGA 3 cut(s) 335, 471, 622
TspRI CASTG 1 cut(s) 303
VpaK11BI GGWCC 1 cut(s) 556
VspI ATTAAT 1 cut(s) 107
XapI RAATTY 2 cut(s) 691, 703
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 2 cut(s) 75, 576
ZraI GACGTC 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.