pycom09g11650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002047
Physical Location & Seq
Reverse (-)
9866819 .. 9867443
625 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g11650.2

Sequence Viewer

Length: 315 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGTCGGGATCACGAACTATTACATGAAAAATGGACGTAAGAACAAGGAGAATGACAAGTTCAAAGTTTGGCCGGAGCTTTCGGGGCTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCAAGAAATTCCGGCAAAACACTATTTCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTGAACAGGGCGCGTGGGGGCGCGTGAGAGCTCCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

105

Amino Acids

12.03

Weight (kDa)

11.0

Isoelectric Point (pI)

45.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 201
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 67, 139
AluBI AGCT 5 cut(s) 17, 28, 49, 154, 305
AluI AGCT 5 cut(s) 17, 28, 49, 154, 305
Alw21I GWGCWC 1 cut(s) 307
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 269
ApoI RAATTY 1 cut(s) 201
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 5 cut(s) 261, 263, 274, 287, 297
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 307
BbsI GAAGAC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 307
BbvI GCAGC 1 cut(s) 281
BceAI ACGGC 1 cut(s) 197
BisI GCNGC 1 cut(s) 270
BlsI GCNGC 1 cut(s) 271
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 260
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 179
Bse1I ACTGG 1 cut(s) 232
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 232
BsePI GCGCGC 1 cut(s) 259
BseXI GCAGC 1 cut(s) 281
Bsh1236I CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 5 cut(s) 149, 170, 206, 223, 237
BsnI GGCC 1 cut(s) 148
Bsp1286I GDGCHC 1 cut(s) 307
Bsp143I GATC 1 cut(s) 84
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 232
BssECI CCNNGG 1 cut(s) 179
BssHII GCGCGC 1 cut(s) 259
BssMI GATC 1 cut(s) 84
BssNI GRCGYC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 261
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 179
BstFNI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BstHHI GCGC 5 cut(s) 261, 263, 274, 287, 297
BstKTI GATC 1 cut(s) 87
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 55, 160, 269
BstUI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
BstV1I GCAGC 1 cut(s) 281
BstV2I GAAGAC 1 cut(s) 260
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 261
CfoI GCGC 5 cut(s) 261, 263, 274, 287, 297
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 251, 265
Csp6I GTAC 1 cut(s) 5
CviAII CATG 1 cut(s) 100
CviJI RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 305
CviKI_1 RGCY 7 cut(s) 17, 28, 49, 148, 154, 163, 305
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 146
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 307
Eco47I GGWCC 1 cut(s) 37
Eco53kI GAGCTC 1 cut(s) 305
Eco81I CCTNAGG 1 cut(s) 41
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 37
EcoT38I GRGCYC 1 cut(s) 307
FaeI CATG 1 cut(s) 103
FaiI YATR 1 cut(s) 101
FatI CATG 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 270
FriOI GRGCYC 1 cut(s) 307
Fsp4HI GCNGC 1 cut(s) 270
GlaI GCGC 5 cut(s) 260, 262, 273, 286, 296
GluI GCNGC 1 cut(s) 270
HaeIII GGCC 1 cut(s) 148
HapII CCGG 5 cut(s) 149, 170, 206, 223, 237
HgaI GACGC 2 cut(s) 251, 265
HhaI GCGC 5 cut(s) 261, 263, 274, 287, 297
Hin1I GRCGYC 1 cut(s) 78
Hin1II CATG 1 cut(s) 103
Hin6I GCGC 5 cut(s) 259, 261, 272, 285, 295
HinP1I GCGC 5 cut(s) 259, 261, 272, 285, 295
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 175
HpaII CCGG 5 cut(s) 149, 170, 206, 223, 237
HphI GGTGA 1 cut(s) 44
Hpy166II GTNNAC 1 cut(s) 278
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 3 cut(s) 82, 88, 197
Hpy8I GTNNAC 1 cut(s) 278
Hpy99I CGWCG 2 cut(s) 83, 245
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 2 cut(s) 78, 112
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 160, 269
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 2 cut(s) 78, 112
Hsp92I GRCGYC 1 cut(s) 78
Hsp92II CATG 1 cut(s) 103
HspAI GCGC 5 cut(s) 259, 261, 272, 285, 295
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 2 cut(s) 151, 310
LpnPI CCDG 7 cut(s) 162, 183, 219, 236, 245, 250, 266
Lsp1109I GCAGC 1 cut(s) 281
MaeII ACGT 2 cut(s) 78, 112
MalI GATC 1 cut(s) 86
MauBI CGCGCGCG 1 cut(s) 259
MboI GATC 1 cut(s) 84
MboII GAAGA 2 cut(s) 262, 265
MhlI GDGCHC 1 cut(s) 307
MluCI AATT 3 cut(s) 9, 201, 310
MnlI CCTC 2 cut(s) 28, 240
MspI CCGG 5 cut(s) 149, 170, 206, 223, 237
MvnI CGCG 7 cut(s) 245, 259, 261, 263, 274, 287, 297
MwoI GCNNNNNNNGC 3 cut(s) 55, 160, 269
NdeII GATC 1 cut(s) 84
NlaIII CATG 1 cut(s) 103
NlaIV GGNNCC 1 cut(s) 6
PauI GCGCGC 1 cut(s) 259
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 271
PpuMI RGGWCCY 1 cut(s) 37
Psp124BI GAGCTC 1 cut(s) 307
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 259
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SacI GAGCTC 1 cut(s) 307
SatI GCNGC 1 cut(s) 270
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 37
SduI GDGCHC 1 cut(s) 307
SetI ASST 8 cut(s) 19, 30, 39, 51, 81, 115, 156, 307
SinI GGWCC 1 cut(s) 37
Sse9I AATT 3 cut(s) 9, 201, 310
SstI GAGCTC 1 cut(s) 307
TaiI ACGT 2 cut(s) 81, 115
TasI AATT 3 cut(s) 9, 201, 310
TfiI GAWTC 1 cut(s) 175
TseI GCWGC 1 cut(s) 269
TspDTI ATGAA 1 cut(s) 116
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 201
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.