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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 26 |
| AflIII | ACRYGT | 1 cut(s) | 74 |
| AspS9I | GGNCC | 1 cut(s) | 44 |
| AsuHPI | GGTGA | 2 cut(s) | 77, 106 |
| AvaII | GGWCC | 1 cut(s) | 44 |
| AxyI | CCTNAGG | 1 cut(s) | 89 |
| BauI | CACGAG | 1 cut(s) | 39 |
| BfaI | CTAG | 1 cut(s) | 18 |
| Bme18I | GGWCC | 1 cut(s) | 44 |
| BmgT120I | GGNCC | 1 cut(s) | 44 |
| Bse21I | CCTNAGG | 1 cut(s) | 89 |
| BseMII | CTCAG | 1 cut(s) | 80 |
| BsiSI | CCGG | 1 cut(s) | 52 |
| BslFI | GGGAC | 1 cut(s) | 85 |
| BsmFI | GGGAC | 1 cut(s) | 85 |
| BspACI | CCGC | 1 cut(s) | 26 |
| BspCNI | CTCAG | 1 cut(s) | 81 |
| BssSI | CACGAG | 1 cut(s) | 39 |
| Bst2BI | CACGAG | 1 cut(s) | 39 |
| BstDEI | CTNAG | 1 cut(s) | 89 |
| BstNSI | RCATGY | 1 cut(s) | 78 |
| Bsu36I | CCTNAGG | 1 cut(s) | 89 |
| Cfr13I | GGNCC | 1 cut(s) | 44 |
| CviAII | CATG | 1 cut(s) | 75 |
| CviJI | RGCY | 2 cut(s) | 36, 55 |
| CviKI_1 | RGCY | 2 cut(s) | 36, 55 |
| DdeI | CTNAG | 1 cut(s) | 89 |
| Eco47I | GGWCC | 1 cut(s) | 44 |
| Eco81I | CCTNAGG | 1 cut(s) | 89 |
| EcoO109I | RGGNCCY | 1 cut(s) | 44 |
| EcoT22I | ATGCAT | 1 cut(s) | 6 |
| FaeI | CATG | 1 cut(s) | 78 |
| FaiI | YATR | 1 cut(s) | 76 |
| FaqI | GGGAC | 1 cut(s) | 85 |
| FatI | CATG | 1 cut(s) | 74 |
| FspBI | CTAG | 1 cut(s) | 18 |
| HapII | CCGG | 1 cut(s) | 52 |
| Hin1II | CATG | 1 cut(s) | 78 |
| HinfI | GANTC | 1 cut(s) | 82 |
| HpaII | CCGG | 1 cut(s) | 52 |
| HphI | GGTGA | 2 cut(s) | 77, 106 |
| Hpy188I | TCNGA | 2 cut(s) | 61, 117 |
| Hpy188III | TCNNGA | 1 cut(s) | 39 |
| HpyCH4V | TGCA | 1 cut(s) | 4 |
| HpyF3I | CTNAG | 1 cut(s) | 89 |
| Hsp92II | CATG | 1 cut(s) | 78 |
| LpnPI | CCDG | 2 cut(s) | 65, 101 |
| MaeI | CTAG | 1 cut(s) | 18 |
| MluCI | AATT | 1 cut(s) | 9 |
| MlyI | GAGTC | 1 cut(s) | 76 |
| MnlI | CCTC | 2 cut(s) | 35, 84 |
| Mph1103I | ATGCAT | 1 cut(s) | 6 |
| MseI | TTAA | 1 cut(s) | 8 |
| MspI | CCGG | 1 cut(s) | 52 |
| NlaIII | CATG | 1 cut(s) | 78 |
| NsiI | ATGCAT | 1 cut(s) | 6 |
| NspI | RCATGY | 1 cut(s) | 78 |
| PciI | ACATGT | 1 cut(s) | 74 |
| PleI | GAGTC | 1 cut(s) | 76 |
| PpsI | GAGTC | 1 cut(s) | 76 |
| PpuMI | RGGWCCY | 1 cut(s) | 44 |
| PscI | ACATGT | 1 cut(s) | 74 |
| Psp5II | RGGWCCY | 1 cut(s) | 44 |
| PspPI | GGNCC | 1 cut(s) | 44 |
| PspPPI | RGGWCCY | 1 cut(s) | 44 |
| SaqAI | TTAA | 1 cut(s) | 8 |
| Sau96I | GGNCC | 1 cut(s) | 44 |
| SchI | GAGTC | 1 cut(s) | 76 |
| SetI | ASST | 2 cut(s) | 46, 90 |
| SgeI | CNNG | 6 cut(s) | 30, 51, 53, 64, 87, 100 |
| SinI | GGWCC | 1 cut(s) | 44 |
| Sse9I | AATT | 1 cut(s) | 9 |
| SsiI | CCGC | 1 cut(s) | 26 |
| SspMI | CTAG | 1 cut(s) | 18 |
| TasI | AATT | 1 cut(s) | 9 |
| Tru1I | TTAA | 1 cut(s) | 8 |
| Tru9I | TTAA | 1 cut(s) | 8 |
| VpaK11BI | GGWCC | 1 cut(s) | 44 |
| XceI | RCATGY | 1 cut(s) | 78 |
| XspI | CTAG | 1 cut(s) | 18 |
| Zsp2I | ATGCAT | 1 cut(s) | 6 |