MD17G1024000.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002047
Physical Location & Seq
Forward (+)
1760820 .. 1762763
1944 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1024000.v1.1.491

Sequence Viewer

Length: 450 bp
ATGACGAAAATGCCCTTGACGTTACTAAGGTGCGTTGGATTGAAAAATGGATGGTGTGGATCAATTGGGACCACACAGGACCCTCCTTCCCTTTCCTTTCCCCGTGCATTCCCTGTCTGTCTCTCCTTCACGATCTCACACCCACGCATACACCCAAATACGTACGGCACACCCCAAACCCTCCAAAAACTCGACGGATCGAGGCAAATAAGGGAATTTCAAGCTTGTAGTGGGCTTTATGAGGTCCTAAGGAAGCTCGGGGACGTTCGTTGGACAGTTTTGGACGTCGGGATCGTGGGTTTCAAAGTTGGCCGAATTCTTGGATTTTTCTCGGTGAGACGTATCCCGAGGACGACCGCATCCAGGGACGCCACGGGGGTTACGACCCGTCGACATATCAGTTCTAGTTTAGCGGTGGTTGGCTCACGAGGTCCTTTTCCGGCTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

150

Amino Acids

16.39

Weight (kDa)

11.07

Isoelectric Point (pI)

46.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 288
AccI GTMKAC 1 cut(s) 391
AciI CCGC 2 cut(s) 357, 413
AclWI GGATC 3 cut(s) 67, 205, 299
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 2 cut(s) 215, 315
AcyI GRCGYC 2 cut(s) 285, 369
AfaI GTAC 1 cut(s) 164
AfiI CCNNNNNNNGG 1 cut(s) 363
AgsI TTSAA 3 cut(s) 43, 221, 304
AjnI CCWGG 1 cut(s) 362
AluBI AGCT 2 cut(s) 224, 256
AluI AGCT 2 cut(s) 224, 256
Alw26I GTCTC 2 cut(s) 125, 331
AlwI GGATC 3 cut(s) 67, 205, 299
Ama87I CYCGRG 2 cut(s) 257, 346
AoxI GGCC 1 cut(s) 310
ApoI RAATTY 2 cut(s) 215, 315
AspS9I GGNCC 4 cut(s) 69, 79, 244, 431
AsuHPI GGTGA 1 cut(s) 346
AvaI CYCGRG 2 cut(s) 257, 346
AvaII GGWCC 4 cut(s) 69, 79, 244, 431
AxyI CCTNAGG 1 cut(s) 248
BauI CACGAG 1 cut(s) 426
BccI CCATC 1 cut(s) 45
BceAI ACGGC 1 cut(s) 181
BciT130I CCWGG 1 cut(s) 364
BciVI GTATCC 1 cut(s) 353
BcoDI GTCTC 2 cut(s) 125, 331
BfaI CTAG 1 cut(s) 405
BfuI GTATCC 1 cut(s) 353
Bme1390I CCNGG 1 cut(s) 364
Bme18I GGWCC 4 cut(s) 69, 79, 244, 431
BmeT110I CYCGRG 2 cut(s) 257, 346
BmgT120I GGNCC 4 cut(s) 69, 79, 244, 431
BmiI GGNNCC 2 cut(s) 70, 81
BmrFI CCNGG 1 cut(s) 364
BmsI GCATC 1 cut(s) 368
BsaAI YACGTR 1 cut(s) 162
BsaHI GRCGYC 2 cut(s) 285, 369
BsaJI CCNNGG 3 cut(s) 347, 363, 372
Bsc4I CCNNNNNNNGG 1 cut(s) 363
Bse21I CCTNAGG 1 cut(s) 248
BseBI CCWGG 1 cut(s) 364
BseDI CCNNGG 3 cut(s) 347, 363, 372
BseGI GGATG 2 cut(s) 56, 359
BseLI CCNNNNNNNGG 1 cut(s) 363
Bsh1285I CGRYCG 1 cut(s) 357
BshFI GGCC 1 cut(s) 312
BsiEI CGRYCG 1 cut(s) 357
BsiHKCI CYCGRG 2 cut(s) 257, 346
BsiSI CCGG 1 cut(s) 440
BsiWI CGTACG 1 cut(s) 162
BslFI GGGAC 3 cut(s) 82, 275, 380
BslI CCNNNNNNNGG 1 cut(s) 363
BsmAI GTCTC 2 cut(s) 125, 331
BsmBI CGTCTC 1 cut(s) 331
BsmFI GGGAC 3 cut(s) 82, 275, 380
BsmI GAATGC 1 cut(s) 107
BsnI GGCC 1 cut(s) 312
BsoBI CYCGRG 2 cut(s) 257, 346
Bsp143I GATC 4 cut(s) 59, 132, 197, 291
BspACI CCGC 2 cut(s) 357, 413
BspANI GGCC 1 cut(s) 312
BspLI GGNNCC 2 cut(s) 70, 81
BspPI GGATC 3 cut(s) 67, 205, 299
BssECI CCNNGG 3 cut(s) 347, 363, 372
BssMI GATC 4 cut(s) 59, 132, 197, 291
BssNI GRCGYC 2 cut(s) 285, 369
BssSI CACGAG 1 cut(s) 426
Bst2BI CACGAG 1 cut(s) 426
Bst2UI CCWGG 1 cut(s) 364
Bst4CI ACNGT 1 cut(s) 277
BstACI GRCGYC 2 cut(s) 285, 369
BstBAI YACGTR 1 cut(s) 162
BstDEI CTNAG 2 cut(s) 26, 248
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 2 cut(s) 56, 359
BstKTI GATC 4 cut(s) 62, 135, 200, 294
BstMAI GTCTC 2 cut(s) 125, 331
BstMBI GATC 4 cut(s) 59, 132, 197, 291
BstMCI CGRYCG 1 cut(s) 357
BstNI CCWGG 1 cut(s) 364
BstSCI CCNGG 1 cut(s) 362
BstSNI TACGTA 1 cut(s) 162
Bsu36I CCTNAGG 1 cut(s) 248
BsuI GTATCC 1 cut(s) 353
BsuRI GGCC 1 cut(s) 312
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 2 cut(s) 56, 359
Cfr13I GGNCC 4 cut(s) 69, 79, 244, 431
CseI GACGC 1 cut(s) 377
Csp6I GTAC 1 cut(s) 163
CviJI RGCY 6 cut(s) 224, 235, 256, 312, 423, 443
CviKI_1 RGCY 6 cut(s) 224, 235, 256, 312, 423, 443
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 26, 248
DpnI GATC 4 cut(s) 61, 134, 199, 293
DpnII GATC 4 cut(s) 59, 132, 197, 291
EaeI YGGCCR 1 cut(s) 310
Eco105I TACGTA 1 cut(s) 162
Eco47I GGWCC 4 cut(s) 69, 79, 244, 431
Eco81I CCTNAGG 1 cut(s) 248
Eco88I CYCGRG 2 cut(s) 257, 346
EcoO109I RGGNCCY 3 cut(s) 79, 244, 431
EcoRI GAATTC 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 362
Esp3I CGTCTC 1 cut(s) 331
FaiI YATR 3 cut(s) 149, 240, 396
FaqI GGGAC 3 cut(s) 82, 275, 380
FblI GTMKAC 1 cut(s) 391
FokI GGATG 2 cut(s) 63, 346
FspBI CTAG 1 cut(s) 405
HaeIII GGCC 1 cut(s) 312
HapII CCGG 1 cut(s) 440
HgaI GACGC 1 cut(s) 377
Hin1I GRCGYC 2 cut(s) 285, 369
HincII GTYRAC 1 cut(s) 392
HindII GTYRAC 1 cut(s) 392
HindIII AAGCTT 1 cut(s) 222
HpaII CCGG 1 cut(s) 440
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 1 cut(s) 392
Hpy188I TCNGA 1 cut(s) 449
Hpy188III TCNNGA 4 cut(s) 130, 289, 346, 426
Hpy8I GTNNAC 1 cut(s) 392
Hpy99I CGWCG 3 cut(s) 197, 290, 393
HpyAV CCTTC 2 cut(s) 96, 136
HpyCH4III ACNGT 1 cut(s) 277
HpyCH4IV ACGT 5 cut(s) 20, 161, 264, 285, 340
HpyCH4V TGCA 1 cut(s) 107
HpyF3I CTNAG 2 cut(s) 26, 248
HpySE526I ACGT 5 cut(s) 20, 161, 264, 285, 340
Hsp92I GRCGYC 2 cut(s) 285, 369
Kzo9I GATC 4 cut(s) 59, 132, 197, 291
LpnPI CCDG 4 cut(s) 62, 126, 349, 376
LweI GCATC 1 cut(s) 368
MaeI CTAG 1 cut(s) 405
MaeII ACGT 5 cut(s) 20, 161, 264, 285, 340
MaeIII GTNAC 2 cut(s) 21, 379
MalI GATC 4 cut(s) 61, 134, 199, 293
MboI GATC 4 cut(s) 59, 132, 197, 291
MfeI CAATTG 1 cut(s) 63
MluCI AATT 3 cut(s) 63, 215, 315
MmeI TCCRAC 2 cut(s) 16, 251
MnlI CCTC 6 cut(s) 93, 191, 195, 235, 342, 422
MspI CCGG 1 cut(s) 440
MspR9I CCNGG 1 cut(s) 364
MunI CAATTG 1 cut(s) 63
Mva1269I GAATGC 1 cut(s) 107
MvaI CCWGG 1 cut(s) 364
NdeII GATC 4 cut(s) 59, 132, 197, 291
NlaIV GGNNCC 2 cut(s) 70, 81
PcsI WCGNNNNNNNCGW 2 cut(s) 291, 380
PctI GAATGC 1 cut(s) 107
Pfl23II CGTACG 1 cut(s) 162
Ppu21I YACGTR 1 cut(s) 162
PpuMI RGGWCCY 3 cut(s) 79, 244, 431
Psp5II RGGWCCY 3 cut(s) 79, 244, 431
Psp6I CCWGG 1 cut(s) 362
PspGI CCWGG 1 cut(s) 362
PspLI CGTACG 1 cut(s) 162
PspN4I GGNNCC 2 cut(s) 70, 81
PspPI GGNCC 4 cut(s) 69, 79, 244, 431
PspPPI RGGWCCY 3 cut(s) 79, 244, 431
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
SalI GTCGAC 1 cut(s) 390
Sau3AI GATC 4 cut(s) 59, 132, 197, 291
Sau96I GGNCC 4 cut(s) 69, 79, 244, 431
ScrFI CCNGG 1 cut(s) 364
SfaNI GCATC 1 cut(s) 368
SinI GGWCC 4 cut(s) 69, 79, 244, 431
SnaBI TACGTA 1 cut(s) 162
Sse9I AATT 3 cut(s) 63, 215, 315
SsiI CCGC 2 cut(s) 357, 413
SspMI CTAG 1 cut(s) 405
StyD4I CCNGG 1 cut(s) 362
TaaI ACNGT 1 cut(s) 277
TaiI ACGT 5 cut(s) 23, 164, 267, 288, 343
TaqI TCGA 3 cut(s) 192, 200, 391
TasI AATT 3 cut(s) 63, 215, 315
TspGWI ACGGA 1 cut(s) 210
VpaK11BI GGWCC 4 cut(s) 69, 79, 244, 431
XapI RAATTY 2 cut(s) 215, 315
XmiI GTMKAC 1 cut(s) 391
XspI CTAG 1 cut(s) 405
ZraI GACGTC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.