pycom09g12460

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0002047
Physical Location & Seq
Reverse (-)
10985613 .. 10986199
587 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g12460.1

Sequence Viewer

Length: 282 bp
ATGGTACCAATTTCAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGATTTGGACGTCGGGATCACGAACGATTATGTGAAAAATGGACGTAAGAACAAGGAGAATGACAAGTTCAAAGTTTGGCCGGAGCTTTCGGGGCTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCGAGGAAGAAATTCCGGCAAAACACCATCTCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

94

Amino Acids

10.45

Weight (kDa)

10.75

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 81
Acc65I GGTACC 1 cut(s) 4
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 5 cut(s) 249, 263, 265, 267, 278
AclWI GGATC 1 cut(s) 92
AcoI YGGCCR 1 cut(s) 146
AcsI RAATTY 1 cut(s) 205
AcyI GRCGYC 1 cut(s) 78
AfaI GTAC 1 cut(s) 6
AgsI TTSAA 2 cut(s) 67, 139
AluBI AGCT 4 cut(s) 17, 28, 49, 154
AluI AGCT 4 cut(s) 17, 28, 49, 154
AlwI GGATC 1 cut(s) 92
AoxI GGCC 1 cut(s) 146
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 1 cut(s) 205
Asp700I GAANNNNTTC 1 cut(s) 205
Asp718I GGTACC 1 cut(s) 4
AspLEI GCGC 3 cut(s) 265, 267, 278
AspS9I GGNCC 1 cut(s) 37
AsuHPI GGTGA 1 cut(s) 44
AvaII GGWCC 1 cut(s) 37
AxyI CCTNAGG 1 cut(s) 41
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 264
BccI CCATC 1 cut(s) 229
BceAI ACGGC 1 cut(s) 197
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme18I GGWCC 1 cut(s) 37
BmgT120I GGNCC 1 cut(s) 37
BmiI GGNNCC 1 cut(s) 6
BpiI GAAGAC 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 78
BsaJI CCNNGG 1 cut(s) 179
BsaXI ACNNNNNCTCC 2 cut(s) 208, 238
Bse1I ACTGG 1 cut(s) 236
Bse21I CCTNAGG 1 cut(s) 41
BseDI CCNNGG 1 cut(s) 179
BseNI ACTGG 1 cut(s) 236
BsePI GCGCGC 1 cut(s) 263
Bsh1236I CGCG 5 cut(s) 249, 263, 265, 267, 278
BshFI GGCC 1 cut(s) 148
BshNI GGYRCC 1 cut(s) 4
BsiSI CCGG 5 cut(s) 149, 170, 210, 227, 241
BsnI GGCC 1 cut(s) 148
Bsp143I GATC 1 cut(s) 84
BspANI GGCC 1 cut(s) 148
BspFNI CGCG 5 cut(s) 249, 263, 265, 267, 278
BspLI GGNNCC 1 cut(s) 6
BspPI GGATC 1 cut(s) 92
BspT107I GGYRCC 1 cut(s) 4
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 179
BssHII GCGCGC 1 cut(s) 263
BssMI GATC 1 cut(s) 84
BssNI GRCGYC 1 cut(s) 78
BstACI GRCGYC 1 cut(s) 78
BstC8I GCNNGC 1 cut(s) 265
BstDEI CTNAG 1 cut(s) 41
BstDSI CCRYGG 1 cut(s) 179
BstFNI CGCG 5 cut(s) 249, 263, 265, 267, 278
BstHHI GCGC 3 cut(s) 265, 267, 278
BstKTI GATC 1 cut(s) 87
BstMBI GATC 1 cut(s) 84
BstMWI GCNNNNNNNGC 3 cut(s) 55, 160, 273
BstUI CGCG 5 cut(s) 249, 263, 265, 267, 278
BstV2I GAAGAC 1 cut(s) 264
Bsu36I CCTNAGG 1 cut(s) 41
BsuRI GGCC 1 cut(s) 148
BtgI CCRYGG 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 265
CfoI GCGC 3 cut(s) 265, 267, 278
Cfr13I GGNCC 1 cut(s) 37
CseI GACGC 2 cut(s) 255, 269
Csp6I GTAC 1 cut(s) 5
CviJI RGCY 6 cut(s) 17, 28, 49, 148, 154, 163
CviKI_1 RGCY 6 cut(s) 17, 28, 49, 148, 154, 163
CviQI GTAC 1 cut(s) 5
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 86
DpnII GATC 1 cut(s) 84
EaeI YGGCCR 1 cut(s) 146
Eco47I GGWCC 1 cut(s) 37
Eco81I CCTNAGG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 37
FaiI YATR 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 274
Fsp4HI GCNGC 1 cut(s) 274
GlaI GCGC 3 cut(s) 264, 266, 277
GluI GCNGC 1 cut(s) 274
HaeIII GGCC 1 cut(s) 148
HapII CCGG 5 cut(s) 149, 170, 210, 227, 241
HgaI GACGC 2 cut(s) 255, 269
HhaI GCGC 3 cut(s) 265, 267, 278
Hin1I GRCGYC 1 cut(s) 78
Hin6I GCGC 3 cut(s) 263, 265, 276
HinP1I GCGC 3 cut(s) 263, 265, 276
HindIII AAGCTT 1 cut(s) 26
HinfI GANTC 1 cut(s) 175
HpaII CCGG 5 cut(s) 149, 170, 210, 227, 241
HphI GGTGA 1 cut(s) 44
Hpy188I TCNGA 1 cut(s) 53
Hpy188III TCNNGA 2 cut(s) 82, 88
Hpy99I CGWCG 2 cut(s) 83, 249
HpyAV CCTTC 1 cut(s) 61
HpyCH4IV ACGT 2 cut(s) 78, 112
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 160, 273
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 2 cut(s) 78, 112
Hsp92I GRCGYC 1 cut(s) 78
HspAI GCGC 3 cut(s) 263, 265, 276
KpnI GGTACC 1 cut(s) 8
Kzo9I GATC 1 cut(s) 84
LmnI GCTCC 1 cut(s) 151
LpnPI CCDG 6 cut(s) 162, 183, 223, 240, 249, 254
MaeII ACGT 2 cut(s) 78, 112
MalI GATC 1 cut(s) 86
MauBI CGCGCGCG 1 cut(s) 263
MboI GATC 1 cut(s) 84
MboII GAAGA 3 cut(s) 213, 266, 269
MluCI AATT 2 cut(s) 9, 205
MnlI CCTC 3 cut(s) 28, 191, 244
MroXI GAANNNNTTC 1 cut(s) 205
MspI CCGG 5 cut(s) 149, 170, 210, 227, 241
MvnI CGCG 5 cut(s) 249, 263, 265, 267, 278
MwoI GCNNNNNNNGC 3 cut(s) 55, 160, 273
NdeII GATC 1 cut(s) 84
NlaIV GGNNCC 1 cut(s) 6
PauI GCGCGC 1 cut(s) 263
PdmI GAANNNNTTC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 175
PkrI GCNGC 1 cut(s) 275
PpuMI RGGWCCY 1 cut(s) 37
Psp5II RGGWCCY 1 cut(s) 37
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 37
PspPPI RGGWCCY 1 cut(s) 37
PteI GCGCGC 1 cut(s) 263
RsaI GTAC 1 cut(s) 6
RsaNI GTAC 1 cut(s) 5
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 1 cut(s) 84
Sau96I GGNCC 1 cut(s) 37
SetI ASST 7 cut(s) 19, 30, 39, 51, 81, 115, 156
SinI GGWCC 1 cut(s) 37
Sse9I AATT 2 cut(s) 9, 205
TaiI ACGT 2 cut(s) 81, 115
TaqI TCGA 1 cut(s) 196
TasI AATT 2 cut(s) 9, 205
TfiI GAWTC 1 cut(s) 175
TseI GCWGC 1 cut(s) 273
VpaK11BI GGWCC 1 cut(s) 37
XapI RAATTY 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 205
ZraI GACGTC 1 cut(s) 79
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.