pycom03g06560

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0002047
Physical Location & Seq
Reverse (-)
5112175 .. 5112609
435 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g06560.2

Sequence Viewer

Length: 246 bp
ATGTCGATTCGAGCATGTTTTTGGGATTTTGAAGCTTGTAGGAAGCTTGGTGAGGTCCTAAGGAAGCTCGGAGTGCTTCGTTTGAAGGAATTGGACGTCGGGATCATCGTGGTCAAAGGGTCAAGGCAGAAATTCCGGCAAAACACCCACCGCCGGCGAAACCAGTCCGGCGACGCGAGGAAGAAGACGCGCGCGTGGGCAGCGCGTGAACAGGGCGCGTGGGGGCCCGTGAGAGCTCCAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

82

Amino Acids

9.38

Weight (kDa)

11.52

Isoelectric Point (pI)

50.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccII CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
AciI CCGC 1 cut(s) 151
AclWI GGATC 1 cut(s) 110
AcsI RAATTY 1 cut(s) 131
AcyI GRCGYC 1 cut(s) 96
AfiI CCNNNNNNNGG 1 cut(s) 153
AgsI TTSAA 2 cut(s) 32, 85
AluBI AGCT 4 cut(s) 35, 46, 67, 236
AluI AGCT 4 cut(s) 35, 46, 67, 236
Alw21I GWGCWC 1 cut(s) 238
AlwI GGATC 1 cut(s) 110
AoxI GGCC 1 cut(s) 224
ApaI GGGCCC 1 cut(s) 228
ApeKI GCWGC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 131
AspLEI GCGC 4 cut(s) 192, 194, 205, 218
AspS9I GGNCC 3 cut(s) 55, 224, 225
AsuHPI GGTGA 1 cut(s) 62
AvaII GGWCC 1 cut(s) 55
AxyI CCTNAGG 1 cut(s) 59
BaeGI GKGCMC 1 cut(s) 228
BanII GRGCYC 2 cut(s) 228, 238
BbsI GAAGAC 1 cut(s) 191
Bbv12I GWGCWC 1 cut(s) 238
BbvI GCAGC 1 cut(s) 212
BisI GCNGC 1 cut(s) 201
BlsI GCNGC 1 cut(s) 202
Bme18I GGWCC 1 cut(s) 55
BmgT120I GGNCC 3 cut(s) 55, 224, 225
BmiI GGNNCC 2 cut(s) 225, 226
BpiI GAAGAC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 96
Bsc4I CCNNNNNNNGG 1 cut(s) 153
Bse118I RCCGGY 1 cut(s) 153
Bse1I ACTGG 1 cut(s) 163
Bse21I CCTNAGG 1 cut(s) 59
BseLI CCNNNNNNNGG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 163
BsePI GCGCGC 1 cut(s) 190
BseSI GKGCMC 1 cut(s) 228
BseXI GCAGC 1 cut(s) 212
Bsh1236I CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
BshFI GGCC 1 cut(s) 226
BsiHKAI GWGCWC 1 cut(s) 238
BsiSI CCGG 3 cut(s) 136, 154, 168
BslI CCNNNNNNNGG 1 cut(s) 153
BsnI GGCC 1 cut(s) 226
Bsp120I GGGCCC 1 cut(s) 224
Bsp1286I GDGCHC 2 cut(s) 228, 238
Bsp143I GATC 1 cut(s) 102
BspACI CCGC 1 cut(s) 151
BspANI GGCC 1 cut(s) 226
BspFNI CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
BspLI GGNNCC 2 cut(s) 225, 226
BspPI GGATC 1 cut(s) 110
BsrFI RCCGGY 1 cut(s) 153
BsrI ACTGG 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 153
BssHII GCGCGC 1 cut(s) 190
BssMI GATC 1 cut(s) 102
BssNI GRCGYC 1 cut(s) 96
BstACI GRCGYC 1 cut(s) 96
BstC8I GCNNGC 2 cut(s) 155, 192
BstDEI CTNAG 1 cut(s) 59
BstFNI CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
BstHHI GCGC 4 cut(s) 192, 194, 205, 218
BstKTI GATC 1 cut(s) 105
BstMBI GATC 1 cut(s) 102
BstMWI GCNNNNNNNGC 2 cut(s) 73, 200
BstNSI RCATGY 1 cut(s) 18
BstSLI GKGCMC 1 cut(s) 228
BstUI CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
BstV1I GCAGC 1 cut(s) 212
BstV2I GAAGAC 1 cut(s) 191
Bsu36I CCTNAGG 1 cut(s) 59
BsuRI GGCC 1 cut(s) 226
Cac8I GCNNGC 2 cut(s) 155, 192
CfoI GCGC 4 cut(s) 192, 194, 205, 218
Cfr10I RCCGGY 1 cut(s) 153
Cfr13I GGNCC 3 cut(s) 55, 224, 225
CseI GACGC 2 cut(s) 182, 196
CviAII CATG 1 cut(s) 15
CviJI RGCY 5 cut(s) 35, 46, 67, 226, 236
CviKI_1 RGCY 5 cut(s) 35, 46, 67, 226, 236
DdeI CTNAG 1 cut(s) 59
DpnI GATC 1 cut(s) 104
DpnII GATC 1 cut(s) 102
Ecl136II GAGCTC 1 cut(s) 236
Eco24I GRGCYC 2 cut(s) 228, 238
Eco47I GGWCC 1 cut(s) 55
Eco53kI GAGCTC 1 cut(s) 236
Eco81I CCTNAGG 1 cut(s) 59
EcoICRI GAGCTC 1 cut(s) 236
EcoO109I RGGNCCY 2 cut(s) 55, 224
EcoT38I GRGCYC 2 cut(s) 228, 238
FaeI CATG 1 cut(s) 18
FaiI YATR 1 cut(s) 16
FatI CATG 1 cut(s) 14
Fnu4HI GCNGC 1 cut(s) 201
FriOI GRGCYC 2 cut(s) 228, 238
Fsp4HI GCNGC 1 cut(s) 201
GlaI GCGC 4 cut(s) 191, 193, 204, 217
GluI GCNGC 1 cut(s) 201
HaeIII GGCC 1 cut(s) 226
HapII CCGG 3 cut(s) 136, 154, 168
HgaI GACGC 2 cut(s) 182, 196
HhaI GCGC 4 cut(s) 192, 194, 205, 218
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 1 cut(s) 18
Hin6I GCGC 4 cut(s) 190, 192, 203, 216
HinP1I GCGC 4 cut(s) 190, 192, 203, 216
HindIII AAGCTT 2 cut(s) 33, 44
HinfI GANTC 1 cut(s) 7
HpaII CCGG 3 cut(s) 136, 154, 168
HphI GGTGA 1 cut(s) 62
Hpy166II GTNNAC 1 cut(s) 209
Hpy188I TCNGA 1 cut(s) 71
Hpy188III TCNNGA 1 cut(s) 100
Hpy8I GTNNAC 1 cut(s) 209
Hpy99I CGWCG 2 cut(s) 101, 176
HpyAV CCTTC 1 cut(s) 79
HpyCH4IV ACGT 1 cut(s) 96
HpyF10VI GCNNNNNNNGC 2 cut(s) 73, 200
HpyF3I CTNAG 1 cut(s) 59
HpySE526I ACGT 1 cut(s) 96
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 1 cut(s) 18
HspAI GCGC 4 cut(s) 190, 192, 203, 216
KroI GCCGGC 1 cut(s) 153
KroNI GCCGGC 1 cut(s) 155
Kzo9I GATC 1 cut(s) 102
LmnI GCTCC 1 cut(s) 241
LpnPI CCDG 5 cut(s) 149, 167, 176, 181, 197
Lsp1109I GCAGC 1 cut(s) 212
MaeII ACGT 1 cut(s) 96
MalI GATC 1 cut(s) 104
MauBI CGCGCGCG 1 cut(s) 190
MboI GATC 1 cut(s) 102
MboII GAAGA 2 cut(s) 193, 196
MhlI GDGCHC 2 cut(s) 228, 238
MluCI AATT 3 cut(s) 89, 131, 241
MnlI CCTC 2 cut(s) 46, 171
MreI CGCCGGCG 1 cut(s) 153
MroNI GCCGGC 1 cut(s) 153
MspI CCGG 3 cut(s) 136, 154, 168
MvnI CGCG 6 cut(s) 176, 190, 192, 194, 205, 218
MwoI GCNNNNNNNGC 2 cut(s) 73, 200
NaeI GCCGGC 1 cut(s) 155
NdeII GATC 1 cut(s) 102
NgoMIV GCCGGC 1 cut(s) 153
NlaIII CATG 1 cut(s) 18
NlaIV GGNNCC 2 cut(s) 225, 226
NspI RCATGY 1 cut(s) 18
PauI GCGCGC 1 cut(s) 190
PcsI WCGNNNNNNNCGW 1 cut(s) 105
PdiI GCCGGC 1 cut(s) 155
PfeI GAWTC 1 cut(s) 7
PkrI GCNGC 1 cut(s) 202
PpuMI RGGWCCY 1 cut(s) 55
Psp124BI GAGCTC 1 cut(s) 238
Psp5II RGGWCCY 1 cut(s) 55
PspN4I GGNNCC 2 cut(s) 225, 226
PspOMI GGGCCC 1 cut(s) 224
PspPI GGNCC 3 cut(s) 55, 224, 225
PspPPI RGGWCCY 1 cut(s) 55
PteI GCGCGC 1 cut(s) 190
SacI GAGCTC 1 cut(s) 238
SatI GCNGC 1 cut(s) 201
Sau3AI GATC 1 cut(s) 102
Sau96I GGNCC 3 cut(s) 55, 224, 225
SduI GDGCHC 2 cut(s) 228, 238
SetI ASST 6 cut(s) 37, 48, 57, 69, 99, 238
SgrAI CRCCGGYG 1 cut(s) 153
SinI GGWCC 1 cut(s) 55
Sse9I AATT 3 cut(s) 89, 131, 241
SsiI CCGC 1 cut(s) 151
SstI GAGCTC 1 cut(s) 238
TaiI ACGT 1 cut(s) 99
TaqI TCGA 2 cut(s) 5, 10
TasI AATT 3 cut(s) 89, 131, 241
TfiI GAWTC 1 cut(s) 7
TseI GCWGC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 131
XceI RCATGY 1 cut(s) 18
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.