MD08G1024900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0002047
Physical Location & Seq
Forward (+)
1794364 .. 1796416
2053 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1024900.v1.1.491

Sequence Viewer

Length: 264 bp
ATGACGGAAATACCCTTGACGTTACTAAGGAAGCTTGGTGAGGTCCTAACGAGGCTCGGAGTGCTTCGTTTGGAAGAATTGGACGTCGGGATCACCTGGTTCGAGTTTGGCCGGTTTTCTTGGATTTTTGGAAGGTATGATCCCGTGATTTTTGGTTCTAGTTTAGCGGTGGGTGGCTCACGAGGTCATTTTCCGGCTTCTAACAGAATTTCGACATGTAGGACTCACCTGAGGGTGATGTATTTTTATTTCAGTCCTACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

88

Amino Acids

9.99

Weight (kDa)

9.61

Isoelectric Point (pI)

41.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 87
AciI CCGC 1 cut(s) 167
AclWI GGATC 2 cut(s) 98, 134
AcoI YGGCCR 1 cut(s) 109
AcsI RAATTY 1 cut(s) 207
AcyI GRCGYC 1 cut(s) 84
AflIII ACRYGT 1 cut(s) 215
AjnI CCWGG 1 cut(s) 95
AluBI AGCT 1 cut(s) 34
AluI AGCT 1 cut(s) 34
AlwI GGATC 2 cut(s) 98, 134
AoxI GGCC 1 cut(s) 109
ApoI RAATTY 1 cut(s) 207
AspS9I GGNCC 1 cut(s) 43
AsuHPI GGTGA 4 cut(s) 50, 85, 218, 247
AvaII GGWCC 1 cut(s) 43
AxyI CCTNAGG 1 cut(s) 230
BauI CACGAG 1 cut(s) 180
BciT130I CCWGG 1 cut(s) 97
BfaI CTAG 1 cut(s) 159
Bme1390I CCNGG 1 cut(s) 97
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 97
BsaHI GRCGYC 1 cut(s) 84
Bse118I RCCGGY 1 cut(s) 111
Bse21I CCTNAGG 1 cut(s) 230
BseBI CCWGG 1 cut(s) 97
BseMII CTCAG 1 cut(s) 221
BshFI GGCC 1 cut(s) 111
BsiSI CCGG 2 cut(s) 112, 194
BsnI GGCC 1 cut(s) 111
Bsp143I GATC 2 cut(s) 90, 139
BspACI CCGC 1 cut(s) 167
BspANI GGCC 1 cut(s) 111
BspCNI CTCAG 1 cut(s) 222
BspPI GGATC 2 cut(s) 98, 134
BsrFI RCCGGY 1 cut(s) 111
BssAI RCCGGY 1 cut(s) 111
BssMI GATC 2 cut(s) 90, 139
BssNI GRCGYC 1 cut(s) 84
BssSI CACGAG 1 cut(s) 180
Bst2BI CACGAG 1 cut(s) 180
Bst2UI CCWGG 1 cut(s) 97
BstACI GRCGYC 1 cut(s) 84
BstDEI CTNAG 2 cut(s) 26, 230
BstKTI GATC 2 cut(s) 93, 142
BstMBI GATC 2 cut(s) 90, 139
BstMWI GCNNNNNNNGC 1 cut(s) 61
BstNI CCWGG 1 cut(s) 97
BstNSI RCATGY 1 cut(s) 219
BstSCI CCNGG 1 cut(s) 95
Bsu36I CCTNAGG 1 cut(s) 230
BsuRI GGCC 1 cut(s) 111
Cfr10I RCCGGY 1 cut(s) 111
Cfr13I GGNCC 1 cut(s) 43
CsiI ACCWGGT 1 cut(s) 95
CviAII CATG 1 cut(s) 216
CviJI RGCY 5 cut(s) 34, 55, 111, 177, 197
CviKI_1 RGCY 5 cut(s) 34, 55, 111, 177, 197
DdeI CTNAG 2 cut(s) 26, 230
DpnI GATC 2 cut(s) 92, 141
DpnII GATC 2 cut(s) 90, 139
EaeI YGGCCR 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 43
Eco81I CCTNAGG 1 cut(s) 230
EcoO109I RGGNCCY 1 cut(s) 43
EcoRII CCWGG 1 cut(s) 95
FaeI CATG 1 cut(s) 219
FaiI YATR 2 cut(s) 138, 217
FatI CATG 1 cut(s) 215
FspBI CTAG 1 cut(s) 159
HaeIII GGCC 1 cut(s) 111
HapII CCGG 2 cut(s) 112, 194
Hin1I GRCGYC 1 cut(s) 84
Hin1II CATG 1 cut(s) 219
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 1 cut(s) 223
HpaII CCGG 2 cut(s) 112, 194
HphI GGTGA 4 cut(s) 50, 85, 218, 247
Hpy188I TCNGA 1 cut(s) 59
Hpy188III TCNNGA 2 cut(s) 88, 180
Hpy99I CGWCG 1 cut(s) 89
HpyAV CCTTC 1 cut(s) 126
HpyCH4IV ACGT 2 cut(s) 20, 84
HpyF10VI GCNNNNNNNGC 1 cut(s) 61
HpyF3I CTNAG 2 cut(s) 26, 230
HpySE526I ACGT 2 cut(s) 20, 84
Hsp92I GRCGYC 1 cut(s) 84
Hsp92II CATG 1 cut(s) 219
Kzo9I GATC 2 cut(s) 90, 139
LpnPI CCDG 5 cut(s) 82, 109, 125, 207, 242
MabI ACCWGGT 1 cut(s) 95
MaeI CTAG 1 cut(s) 159
MaeII ACGT 2 cut(s) 20, 84
MaeIII GTNAC 1 cut(s) 21
MalI GATC 2 cut(s) 92, 141
MboI GATC 2 cut(s) 90, 139
MboII GAAGA 1 cut(s) 86
MluCI AATT 2 cut(s) 77, 207
MlyI GAGTC 1 cut(s) 217
MnlI CCTC 4 cut(s) 34, 45, 176, 225
MspI CCGG 2 cut(s) 112, 194
MspR9I CCNGG 1 cut(s) 97
MvaI CCWGG 1 cut(s) 97
MwoI GCNNNNNNNGC 1 cut(s) 61
NdeII GATC 2 cut(s) 90, 139
NlaIII CATG 1 cut(s) 219
NspI RCATGY 1 cut(s) 219
PciI ACATGT 1 cut(s) 215
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 1 cut(s) 43
PscI ACATGT 1 cut(s) 215
Psp5II RGGWCCY 1 cut(s) 43
Psp6I CCWGG 1 cut(s) 95
PspGI CCWGG 1 cut(s) 95
PspPI GGNCC 1 cut(s) 43
PspPPI RGGWCCY 1 cut(s) 43
Sau3AI GATC 2 cut(s) 90, 139
Sau96I GGNCC 1 cut(s) 43
SchI GAGTC 1 cut(s) 217
ScrFI CCNGG 1 cut(s) 97
SetI ASST 8 cut(s) 23, 36, 45, 87, 98, 137, 187, 231
SexAI ACCWGGT 1 cut(s) 95
SinI GGWCC 1 cut(s) 43
Sse9I AATT 2 cut(s) 77, 207
SsiI CCGC 1 cut(s) 167
SspMI CTAG 1 cut(s) 159
StyD4I CCNGG 1 cut(s) 95
TaiI ACGT 2 cut(s) 23, 87
TaqI TCGA 2 cut(s) 102, 212
TasI AATT 2 cut(s) 77, 207
TspGWI ACGGA 1 cut(s) 20
VpaK11BI GGWCC 1 cut(s) 43
XapI RAATTY 1 cut(s) 207
XceI RCATGY 1 cut(s) 219
XspI CTAG 1 cut(s) 159
ZraI GACGTC 1 cut(s) 85
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.