pycom12g11120

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0002047
Physical Location & Seq
Forward (+)
13219405 .. 13220148
744 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g11120.3

Sequence Viewer

Length: 522 bp
ATGATTAAAGACAGGGCACTGTGCATACATGGGTTGCACATTTCTTCTCCTTTTCCTTTTCCTTTCCGTGATTTCCTCAAACCTGTTTTTCATTCTTCCTCTCTCCCCGTCAAGCTCTCTCTTTCTCCCTCACTCTCAAACACACGGCAACAACACCAATTCTTCAAAACTTCACGGATCGATGAAAATAAAATCACCATCTTCTTCATTTCGACGATACGAGTCCAACCATACCAATTTCAGGAGGCTTGGAGTAATTCGTTGGAAGTTTTTGGACAAAAAAACACGGAGAAAGACAAGTTCAAAGTTTGGCCGGAGCTTTCGAGGTTTTTTCCGGCGAATCCACGGCGAGTTAGGGGTCGAGGCAGAAATTCCGGCAAAACACCCACCTCCGGCGAAACCAGTCCGGCGACGCGAAGAAGAAGACGCGCGCGTGGGCAGCGCGTATACAGGGCGCGTGAGAGCTCTAAATTGAGTTCTAAAAATTGTCATGTGTTCGTGACGTCGTGTAGAGCACCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

174

Amino Acids

20.07

Weight (kDa)

11.44

Isoelectric Point (pI)

59.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 506
AccI GTMKAC 1 cut(s) 447
AccII CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
AclWI GGATC 1 cut(s) 185
AcoI YGGCCR 1 cut(s) 311
AcsI RAATTY 1 cut(s) 370
AcyI GRCGYC 1 cut(s) 503
AfiI CCNNNNNNNGG 2 cut(s) 241, 392
AgsI TTSAA 2 cut(s) 166, 304
AluBI AGCT 3 cut(s) 115, 319, 465
AluI AGCT 3 cut(s) 115, 319, 465
Alw21I GWGCWC 2 cut(s) 467, 517
AlwI GGATC 1 cut(s) 185
AoxI GGCC 1 cut(s) 311
ApeKI GCWGC 1 cut(s) 439
ApoI RAATTY 1 cut(s) 370
AspLEI GCGC 4 cut(s) 431, 433, 444, 457
AsuHPI GGTGA 1 cut(s) 187
BaeGI GKGCMC 1 cut(s) 19
BanII GRGCYC 1 cut(s) 467
BbsI GAAGAC 1 cut(s) 430
Bbv12I GWGCWC 2 cut(s) 467, 517
BbvI GCAGC 1 cut(s) 451
BccI CCATC 1 cut(s) 206
BceAI ACGGC 2 cut(s) 161, 362
BisI GCNGC 1 cut(s) 440
BlsI GCNGC 1 cut(s) 441
BpiI GAAGAC 1 cut(s) 430
Bsa29I ATCGAT 1 cut(s) 180
BsaHI GRCGYC 1 cut(s) 503
BsaJI CCNNGG 1 cut(s) 344
Bsc4I CCNNNNNNNGG 2 cut(s) 241, 392
Bse1I ACTGG 1 cut(s) 402
BseCI ATCGAT 1 cut(s) 180
BseDI CCNNGG 1 cut(s) 344
BseLI CCNNNNNNNGG 2 cut(s) 241, 392
BseNI ACTGG 1 cut(s) 402
BsePI GCGCGC 1 cut(s) 429
BseSI GKGCMC 1 cut(s) 19
BseXI GCAGC 1 cut(s) 451
Bsh1236I CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
BshFI GGCC 1 cut(s) 313
BshVI ATCGAT 1 cut(s) 180
BsiHKAI GWGCWC 2 cut(s) 467, 517
BsiSI CCGG 5 cut(s) 314, 335, 375, 393, 407
BslI CCNNNNNNNGG 2 cut(s) 241, 392
BsnI GGCC 1 cut(s) 313
Bsp1286I GDGCHC 3 cut(s) 19, 467, 517
Bsp143I GATC 1 cut(s) 177
BspANI GGCC 1 cut(s) 313
BspDI ATCGAT 1 cut(s) 180
BspFNI CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
BspPI GGATC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 402
BssECI CCNNGG 1 cut(s) 344
BssHII GCGCGC 1 cut(s) 429
BssMI GATC 1 cut(s) 177
BssNAI GTATAC 1 cut(s) 448
BssNI GRCGYC 1 cut(s) 503
Bst1107I GTATAC 1 cut(s) 448
Bst4CI ACNGT 2 cut(s) 21, 519
BstACI GRCGYC 1 cut(s) 503
BstC8I GCNNGC 1 cut(s) 431
BstDSI CCRYGG 1 cut(s) 344
BstFNI CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
BstHHI GCGC 4 cut(s) 431, 433, 444, 457
BstKTI GATC 1 cut(s) 180
BstMBI GATC 1 cut(s) 177
BstMWI GCNNNNNNNGC 1 cut(s) 439
BstSLI GKGCMC 1 cut(s) 19
BstUI CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
BstV1I GCAGC 1 cut(s) 451
BstV2I GAAGAC 1 cut(s) 430
BstZ17I GTATAC 1 cut(s) 448
Bsu15I ATCGAT 1 cut(s) 180
BsuRI GGCC 1 cut(s) 313
BsuTUI ATCGAT 1 cut(s) 180
BtgI CCRYGG 1 cut(s) 344
BtsIMutI CAGTG 1 cut(s) 17
Cac8I GCNNGC 1 cut(s) 431
CfoI GCGC 4 cut(s) 431, 433, 444, 457
ClaI ATCGAT 1 cut(s) 180
CseI GACGC 2 cut(s) 421, 435
CviAII CATG 2 cut(s) 29, 491
CviJI RGCY 5 cut(s) 115, 248, 313, 319, 465
CviKI_1 RGCY 5 cut(s) 115, 248, 313, 319, 465
DpnI GATC 1 cut(s) 179
DpnII GATC 1 cut(s) 177
EaeI YGGCCR 1 cut(s) 311
Ecl136II GAGCTC 1 cut(s) 465
Eco24I GRGCYC 1 cut(s) 467
Eco53kI GAGCTC 1 cut(s) 465
EcoICRI GAGCTC 1 cut(s) 465
EcoT38I GRGCYC 1 cut(s) 467
FaeI CATG 2 cut(s) 32, 494
FaiI YATR 5 cut(s) 26, 30, 232, 448, 492
FatI CATG 2 cut(s) 28, 490
FblI GTMKAC 1 cut(s) 447
Fnu4HI GCNGC 1 cut(s) 440
FriOI GRGCYC 1 cut(s) 467
Fsp4HI GCNGC 1 cut(s) 440
GlaI GCGC 4 cut(s) 430, 432, 443, 456
GluI GCNGC 1 cut(s) 440
HaeIII GGCC 1 cut(s) 313
HapII CCGG 5 cut(s) 314, 335, 375, 393, 407
HgaI GACGC 2 cut(s) 421, 435
HhaI GCGC 4 cut(s) 431, 433, 444, 457
Hin1I GRCGYC 1 cut(s) 503
Hin1II CATG 2 cut(s) 32, 494
Hin6I GCGC 4 cut(s) 429, 431, 442, 455
HinP1I GCGC 4 cut(s) 429, 431, 442, 455
HinfI GANTC 2 cut(s) 222, 340
HpaII CCGG 5 cut(s) 314, 335, 375, 393, 407
HphI GGTGA 1 cut(s) 187
Hpy166II GTNNAC 1 cut(s) 448
Hpy188III TCNNGA 2 cut(s) 242, 499
Hpy8I GTNNAC 1 cut(s) 448
Hpy99I CGWCG 3 cut(s) 217, 415, 508
HpyCH4III ACNGT 2 cut(s) 21, 519
HpyCH4IV ACGT 1 cut(s) 503
HpyCH4V TGCA 2 cut(s) 24, 37
HpyF10VI GCNNNNNNNGC 1 cut(s) 439
HpySE526I ACGT 1 cut(s) 503
Hsp92I GRCGYC 1 cut(s) 503
Hsp92II CATG 2 cut(s) 32, 494
HspAI GCGC 4 cut(s) 429, 431, 442, 455
Kzo9I GATC 1 cut(s) 177
LmnI GCTCC 1 cut(s) 316
LpnPI CCDG 9 cut(s) 96, 227, 327, 348, 388, 406, 415, 420, 436
Lsp1109I GCAGC 1 cut(s) 451
MaeII ACGT 1 cut(s) 503
MaeIII GTNAC 1 cut(s) 499
MalI GATC 1 cut(s) 179
MauBI CGCGCGCG 1 cut(s) 429
MboI GATC 1 cut(s) 177
MboII GAAGA 8 cut(s) 36, 87, 154, 193, 196, 429, 432, 435
MhlI GDGCHC 3 cut(s) 19, 467, 517
MluCI AATT 6 cut(s) 158, 236, 256, 370, 470, 484
MlyI GAGTC 1 cut(s) 231
MmeI TCCRAC 2 cut(s) 243, 250
MnlI CCTC 7 cut(s) 86, 109, 139, 238, 318, 356, 400
MseI TTAA 1 cut(s) 6
MspI CCGG 5 cut(s) 314, 335, 375, 393, 407
MvnI CGCG 6 cut(s) 415, 429, 431, 433, 444, 457
MwoI GCNNNNNNNGC 1 cut(s) 439
NdeII GATC 1 cut(s) 177
NlaIII CATG 2 cut(s) 32, 494
NmuCI GTSAC 1 cut(s) 499
PauI GCGCGC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 340
PkrI GCNGC 1 cut(s) 441
PleI GAGTC 1 cut(s) 230
PpsI GAGTC 1 cut(s) 230
Psp124BI GAGCTC 1 cut(s) 467
PteI GCGCGC 1 cut(s) 429
SacI GAGCTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 6
SatI GCNGC 1 cut(s) 440
Sau3AI GATC 1 cut(s) 177
SchI GAGTC 1 cut(s) 231
SduI GDGCHC 3 cut(s) 19, 467, 517
SetI ASST 7 cut(s) 85, 117, 321, 329, 392, 467, 506
Sse9I AATT 6 cut(s) 158, 236, 256, 370, 470, 484
SstI GAGCTC 1 cut(s) 467
TaaI ACNGT 2 cut(s) 21, 519
TaiI ACGT 1 cut(s) 506
TaqI TCGA 4 cut(s) 180, 212, 323, 361
TasI AATT 6 cut(s) 158, 236, 256, 370, 470, 484
TfiI GAWTC 1 cut(s) 340
Tru1I TTAA 1 cut(s) 6
Tru9I TTAA 1 cut(s) 6
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 499
TseI GCWGC 1 cut(s) 439
Tsp45I GTSAC 1 cut(s) 499
TspDTI ATGAA 3 cut(s) 80, 196, 198
TspGWI ACGGA 3 cut(s) 56, 190, 302
TspRI CASTG 1 cut(s) 24
XapI RAATTY 1 cut(s) 370
XmiI GTMKAC 1 cut(s) 447
ZraI GACGTC 1 cut(s) 504
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.