Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0001543

Physical Location & Seq

Genomic Coordinates Forward (+)

13522014 .. 13523508

Gene Structure 1495 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1065100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 654 bp

ATGGAGGTTCTCAGACGCGGCCGGCCATTCCTCTTTCCCCAGCTCGCTTTCTGGGTCTTCGTCATACCCTCTTCGTCTCTCACTTCGTCTTCGCTTTCCCACTTCTTTGGGAATGTTCGTATCCGCTCCAACTCCTCAAAGTTCTCTAATTTGCGCCAACAGAGCCGAAGAAATCTTTCTGTTATTGCCATGGCTGCTGAGGACGGAAAGCGTGCAGTACCATTAGAAGATTATCGCAATATAGGGATTATGGCTCACATAGATGCAGGGAAGACTACTACAACGGAACAAATTTTGTTTTATACTGGAAGAAACTATAAAATAGGGGAGGTACACGAAGGAACGGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACATCTGCTGCAACTACAACATTTTGGGACAACCATAGGATTAACATCATTGACACTCCTGGCCACGTTGACTTTACCCTTGAAGTGGAGCGGGCTCTCAGGGTGCTGGATGGCACTATATGCTTGTTTGACAGTGTTGCTGGTGTGGAACGTCAATCTGAAACTGTGTGGAGGCAAGCTGATAGATATGGGGTGCCCAGGATTTGCTTTGTCAATAAAATGGATCGTCTTGGAGCAAACTTTTTCCGACAAGGGACATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0005488 GO:0005524 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006996 GO:0007275 GO:0008144 GO:0008150 GO:0009507 GO:0009526 GO:0009532 GO:0009536 GO:0009570 GO:0009657 GO:0009658 GO:0009791 GO:0009845 GO:0009941 GO:0009987 GO:0016043 GO:0017076 GO:0030554 GO:0031967 GO:0031975 GO:0032501 GO:0032502 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044444 GO:0044446 GO:0044464 GO:0048046 GO:0048856 GO:0071840 GO:0090351 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

EFG_C EFG_II EFG_IV GTP_EFTU GTP_EFTU_D2

Protein Analysis

218

Amino Acids

24.73

Weight (kDa)

8.66

Isoelectric Point (pI)

54.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
GTP_EFTU	PF00009	77 - 213	8.9e-56	Elongation factor Tu GTP binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001543)

Species	Orthologous Gene IDs
malus_domestica	MD06G1065100.v1.1
pyrus_communis	pycom04g07360
rosa_chinensis	RchiOBHm_Chr2g0151271
rosa_laevigata	RLG00000029209 RLG00000029405
rosa_multiflora	Rmu_sc0003498.1_g000002 Rmu_sc0009409.1_g000008
rosa_roxburghii	Rroxscaffold_1G00017800 Rroxscaffold_1G00024970 Rroxscaffold_1G00030460 Rroxscaffold_2G00106410 Rroxscaffold_2G00133320 Rroxscaffold_3G00220170 Rroxscaffold_4G00313270 Rroxscaffold_5G00339850 Rroxscaffold_6G00418020 Rroxscaffold_7G00175880
rosa_rugosa	Rorug01G0017700 Rorug05G0318400 Rorug07G0300800
rosa_samantha	Rh1CG004700 Rh1CG043500 Rh1DG112600 Rh1DG195700 Rh2AG218100 Rh2AG300700 Rh2AG454300 Rh2AG454400 Rh2AG459600 Rh2AG581500 Rh2BG119400 Rh2BG123700 Rh2BG174600 Rh2BG524000 Rh2CG125300 Rh2CG441400 Rh2CG441500 Rh2DG120900 Rh2DG125900 Rh2DG297900 Rh2DG324900 Rh2DG339600 Rh3AG238400 Rh3BG207600 Rh5AG278100 Rh5AG507700 Rh5BG529200 Rh5CG324500 Rh7BG426800 Rh7BG450300
rosa_wichuraiana	Rw5G015030

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	586
AccBSI	CCGCTC	2 cut(s)	126, 484
AccII	CGCG	1 cut(s)	18
AciI	CCGC	3 cut(s)	18, 124, 484
AclWI	GGATC	1 cut(s)	624
AcoI	YGGCCR	3 cut(s)	19, 23, 454
AcsI	RAATTY	1 cut(s)	291
AfaI	GTAC	2 cut(s)	219, 333
AfiI	CCNNNNNNNGG	1 cut(s)	478
AgsI	TTSAA	1 cut(s)	476
AjnI	CCWGG	2 cut(s)	451, 590
AluBI	AGCT	2 cut(s)	43, 572
AluI	AGCT	2 cut(s)	43, 572
Alw26I	GTCTC	1 cut(s)	81
AlwI	GGATC	1 cut(s)	624
AoxI	GGCC	3 cut(s)	19, 23, 454
ApeKI	GCWGC	2 cut(s)	194, 401
ApoI	RAATTY	1 cut(s)	291
Asp700I	GAANNNNTTC	1 cut(s)	175
AspLEI	GCGC	1 cut(s)	156
BaeGI	GKGCMC	1 cut(s)	591
BalI	TGGCCA	1 cut(s)	456
BanI	GGYRCC	1 cut(s)	586
BanII	GRGCYC	1 cut(s)	490
BbsI	GAAGAC	3 cut(s)	49, 81, 278
BbvCI	CCTCAGC	1 cut(s)	198
BbvI	GCAGC	2 cut(s)	181, 388
BccI	CCATC	2 cut(s)	355, 497
BceAI	ACGGC	1 cut(s)	360
BciT130I	CCWGG	2 cut(s)	453, 592
BciVI	GTATCC	1 cut(s)	131
BcoDI	GTCTC	1 cut(s)	81
BfuI	GTATCC	1 cut(s)	131
BisI	GCNGC	3 cut(s)	19, 195, 402
BlsI	GCNGC	3 cut(s)	20, 196, 403
Bme1390I	CCNGG	2 cut(s)	453, 592
BmiI	GGNNCC	1 cut(s)	588
BmrFI	CCNGG	2 cut(s)	453, 592
BmsI	GCATC	1 cut(s)	253
BpiI	GAAGAC	3 cut(s)	49, 81, 278
Bpu10I	CCTNAGC	1 cut(s)	198
BsaBI	GATNNNNATC	1 cut(s)	437
BsaJI	CCNNGG	2 cut(s)	189, 590
Bsc4I	CCNNNNNNNGG	1 cut(s)	478
Bse118I	RCCGGY	1 cut(s)	21
Bse1I	ACTGG	2 cut(s)	310, 362
Bse8I	GATNNNNATC	1 cut(s)	437
BseBI	CCWGG	2 cut(s)	453, 592
BseDI	CCNNGG	2 cut(s)	189, 590
BseGI	GGATG	2 cut(s)	366, 508
BseJI	GATNNNNATC	1 cut(s)	437
BseLI	CCNNNNNNNGG	1 cut(s)	478
BseMII	CTCAG	3 cut(s)	25, 189, 505
BseNI	ACTGG	2 cut(s)	310, 362
BseRI	GAGGAG	1 cut(s)	124
BseSI	GKGCMC	1 cut(s)	591
BseX3I	CGGCCG	1 cut(s)	19
BseXI	GCAGC	2 cut(s)	181, 388
BseYI	CCCAGC	1 cut(s)	39
BsgI	GTGCAG	1 cut(s)	234
Bsh1236I	CGCG	1 cut(s)	18
Bsh1285I	CGRYCG	1 cut(s)	22
BshFI	GGCC	3 cut(s)	21, 25, 456
BshNI	GGYRCC	1 cut(s)	586
BsiEI	CGRYCG	1 cut(s)	22
BsiSI	CCGG	1 cut(s)	22
BslFI	GGGAC	1 cut(s)	434
BslI	CCNNNNNNNGG	1 cut(s)	478
BsmAI	GTCTC	1 cut(s)	81
BsmBI	CGTCTC	1 cut(s)	81
BsmFI	GGGAC	1 cut(s)	434
BsnI	GGCC	3 cut(s)	21, 25, 456
Bsp1286I	GDGCHC	2 cut(s)	490, 591
Bsp143I	GATC	1 cut(s)	616
Bsp19I	CCATGG	1 cut(s)	189
BspACI	CCGC	3 cut(s)	18, 124, 484
BspANI	GGCC	3 cut(s)	21, 25, 456
BspCNI	CTCAG	3 cut(s)	24, 190, 504
BspFNI	CGCG	1 cut(s)	18
BspLI	GGNNCC	1 cut(s)	588
BspPI	GGATC	1 cut(s)	624
BspT107I	GGYRCC	1 cut(s)	586
BsrBI	CCGCTC	2 cut(s)	126, 484
BsrFI	RCCGGY	1 cut(s)	21
BsrI	ACTGG	2 cut(s)	310, 362
BssAI	RCCGGY	1 cut(s)	21
BssECI	CCNNGG	2 cut(s)	189, 590
BssMI	GATC	1 cut(s)	616
BssT1I	CCWWGG	1 cut(s)	189
Bst2UI	CCWGG	2 cut(s)	453, 592
Bst4CI	ACNGT	2 cut(s)	527, 559
Bst6I	CTCTTC	1 cut(s)	76
BstAPI	GCANNNNNTGC	1 cut(s)	513
BstC8I	GCNNGC	5 cut(s)	23, 45, 213, 486, 570
BstDEI	CTNAG	3 cut(s)	11, 198, 491
BstDSI	CCRYGG	1 cut(s)	189
BstF5I	GGATG	2 cut(s)	366, 508
BstFNI	CGCG	1 cut(s)	18
BstHHI	GCGC	1 cut(s)	156
BstKTI	GATC	1 cut(s)	619
BstMAI	GTCTC	1 cut(s)	81
BstMBI	GATC	1 cut(s)	616
BstMCI	CGRYCG	1 cut(s)	22
BstMWI	GCNNNNNNNGC	3 cut(s)	162, 194, 513
BstNI	CCWGG	2 cut(s)	453, 592
BstSCI	CCNGG	2 cut(s)	451, 590
BstSLI	GKGCMC	1 cut(s)	591
BstUI	CGCG	1 cut(s)	18
BstV1I	GCAGC	2 cut(s)	181, 388
BstV2I	GAAGAC	3 cut(s)	49, 81, 278
BstXI	CCANNNNNNTGG	1 cut(s)	107
BstZI	CGGCCG	1 cut(s)	19
BsuI	GTATCC	1 cut(s)	131
BsuRI	GGCC	3 cut(s)	21, 25, 456
BtgI	CCRYGG	1 cut(s)	189
BtsCI	GGATG	2 cut(s)	366, 508
BtsIMutI	CAGTG	1 cut(s)	532
Cac8I	GCNNGC	5 cut(s)	23, 45, 213, 486, 570
CfoI	GCGC	1 cut(s)	156
Cfr10I	RCCGGY	1 cut(s)	21
CseI	GACGC	1 cut(s)	24
Csp6I	GTAC	2 cut(s)	218, 332
CviAII	CATG	2 cut(s)	190, 651
CviJI	RGCY	9 cut(s)	21, 25, 43, 165, 194, 254, 456, 488, 572
CviKI_1	RGCY	9 cut(s)	21, 25, 43, 165, 194, 254, 456, 488, 572
CviQI	GTAC	2 cut(s)	218, 332
DdeI	CTNAG	3 cut(s)	11, 198, 491
DpnI	GATC	1 cut(s)	618
DpnII	GATC	1 cut(s)	616
EaeI	YGGCCR	3 cut(s)	19, 23, 454
EagI	CGGCCG	1 cut(s)	19
Eam1104I	CTCTTC	1 cut(s)	76
EarI	CTCTTC	1 cut(s)	76
EclXI	CGGCCG	1 cut(s)	19
Eco130I	CCWWGG	1 cut(s)	189
Eco24I	GRGCYC	1 cut(s)	490
Eco52I	CGGCCG	1 cut(s)	19
EcoRII	CCWGG	2 cut(s)	451, 590
EcoT14I	CCWWGG	1 cut(s)	189
EcoT38I	GRGCYC	1 cut(s)	490
ErhI	CCWWGG	1 cut(s)	189
Esp3I	CGTCTC	1 cut(s)	81
FaeI	CATG	2 cut(s)	193, 654
FaqI	GGGAC	1 cut(s)	434
FatI	CATG	2 cut(s)	189, 650
FauI	CCCGC	1 cut(s)	477
Fnu4HI	GCNGC	3 cut(s)	19, 195, 402
FokI	GGATG	2 cut(s)	373, 515
FriOI	GRGCYC	1 cut(s)	490
FseI	GGCCGGCC	1 cut(s)	25
Fsp4HI	GCNGC	3 cut(s)	19, 195, 402
GlaI	GCGC	1 cut(s)	155
GluI	GCNGC	3 cut(s)	19, 195, 402
GsaI	CCCAGC	1 cut(s)	43
HaeIII	GGCC	3 cut(s)	21, 25, 456
HapII	CCGG	1 cut(s)	22
HgaI	GACGC	1 cut(s)	24
HhaI	GCGC	1 cut(s)	156
Hin1II	CATG	2 cut(s)	193, 654
Hin6I	GCGC	1 cut(s)	154
HinP1I	GCGC	1 cut(s)	154
HincII	GTYRAC	1 cut(s)	463
HindII	GTYRAC	1 cut(s)	463
HpaII	CCGG	1 cut(s)	22
Hpy166II	GTNNAC	2 cut(s)	334, 463
Hpy188I	TCNGA	3 cut(s)	14, 553, 641
Hpy8I	GTNNAC	2 cut(s)	334, 463
HpyAV	CCTTC	1 cut(s)	332
HpyCH4III	ACNGT	2 cut(s)	527, 559
HpyCH4IV	ACGT	2 cut(s)	459, 544
HpyCH4V	TGCA	3 cut(s)	215, 266, 404
HpyF10VI	GCNNNNNNNGC	3 cut(s)	162, 194, 513
HpyF3I	CTNAG	3 cut(s)	11, 198, 491
HpySE526I	ACGT	2 cut(s)	459, 544
Hsp92II	CATG	2 cut(s)	193, 654
HspAI	GCGC	1 cut(s)	154
KroI	GCCGGC	1 cut(s)	21
KroNI	GCCGGC	1 cut(s)	23
Kzo9I	GATC	1 cut(s)	616
LmnI	GCTCC	4 cut(s)	131, 364, 481, 626
Lsp1109I	GCAGC	2 cut(s)	181, 388
LweI	GCATC	1 cut(s)	253
MaeII	ACGT	2 cut(s)	459, 544
MalI	GATC	1 cut(s)	618
MbiI	CCGCTC	2 cut(s)	126, 484
MboI	GATC	1 cut(s)	616
MboII	GAAGA	7 cut(s)	49, 63, 81, 180, 239, 283, 321
MhlI	GDGCHC	2 cut(s)	490, 591
MlsI	TGGCCA	1 cut(s)	456
MluCI	AATT	2 cut(s)	148, 291
MluNI	TGGCCA	1 cut(s)	456
MmeI	TCCRAC	1 cut(s)	153
MnlI	CCTC	7 cut(s)	41, 79, 145, 193, 322, 374, 558
Mox20I	TGGCCA	1 cut(s)	456
MroNI	GCCGGC	1 cut(s)	21
MroXI	GAANNNNTTC	1 cut(s)	175
MscI	TGGCCA	1 cut(s)	456
MseI	TTAA	1 cut(s)	435
MslI	CAYNNNNRTG	1 cut(s)	261
Msp20I	TGGCCA	1 cut(s)	456
MspI	CCGG	1 cut(s)	22
MspR9I	CCNGG	2 cut(s)	453, 592
MvaI	CCWGG	2 cut(s)	453, 592
MvnI	CGCG	1 cut(s)	18
MwoI	GCNNNNNNNGC	3 cut(s)	162, 194, 513
NaeI	GCCGGC	1 cut(s)	23
NcoI	CCATGG	1 cut(s)	189
NdeII	GATC	1 cut(s)	616
NgoMIV	GCCGGC	1 cut(s)	21
NlaIII	CATG	2 cut(s)	193, 654
NlaIV	GGNNCC	1 cut(s)	588
PdiI	GCCGGC	1 cut(s)	23
PdmI	GAANNNNTTC	1 cut(s)	175
PkrI	GCNGC	3 cut(s)	20, 196, 403
Psp6I	CCWGG	2 cut(s)	451, 590
PspFI	CCCAGC	1 cut(s)	39
PspGI	CCWGG	2 cut(s)	451, 590
PspN4I	GGNNCC	1 cut(s)	588
RigI	GGCCGGCC	1 cut(s)	25
RsaI	GTAC	2 cut(s)	219, 333
RsaNI	GTAC	2 cut(s)	218, 332
RseI	CAYNNNNRTG	1 cut(s)	261
SaqAI	TTAA	1 cut(s)	435
SatI	GCNGC	3 cut(s)	19, 195, 402
Sau3AI	GATC	1 cut(s)	616
ScrFI	CCNGG	2 cut(s)	453, 592
SduI	GDGCHC	2 cut(s)	490, 591
SetI	ASST	6 cut(s)	9, 45, 333, 462, 547, 574
SfaNI	GCATC	1 cut(s)	253
SmiMI	CAYNNNNRTG	1 cut(s)	261
Sse9I	AATT	2 cut(s)	148, 291
SsiI	CCGC	3 cut(s)	18, 124, 484
StyD4I	CCNGG	2 cut(s)	451, 590
StyI	CCWWGG	1 cut(s)	189
TaaI	ACNGT	2 cut(s)	527, 559
TaiI	ACGT	2 cut(s)	462, 547
TasI	AATT	2 cut(s)	148, 291
TauI	GCSGC	1 cut(s)	21
Tru1I	TTAA	1 cut(s)	435
Tru9I	TTAA	1 cut(s)	435
TscAI	CASTG	1 cut(s)	532
TseI	GCWGC	2 cut(s)	194, 401
TspGWI	ACGGA	2 cut(s)	219, 299
TspRI	CASTG	1 cut(s)	532
XapI	RAATTY	1 cut(s)	291
XmnI	GAANNNNTTC	1 cut(s)	175

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.