Rh5BG529200

Methyltransferase-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001543
Physical Location & Seq
Forward (+)
83329473 .. 83335365
5893 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG529200.1

Sequence Viewer

Length: 675 bp
ATGTATCATCAATTTCAGCAAGCCTCACTGTTCTTGCTGCACTCCTGTAGATATCGAACACCAGCTCATCGATTCGTATCACAACAAGTGGTACTCCTCTCGATAAAGCCATCGTCCCAATCCTCAATCTCTGAGATTAGATTCGGTACTGTGCACGAATTGAAAAGCTTGACCATGGTGGATTCGGATTCTTCTCTGATGCAAATGGAGATTTTGCTCGGGAAAATTGTTATAGCCCAAAAGTTATTAGCACTACAAAATTCAGGGGTGCAGTTGCAAACGACGCTCTTGATGTGTAGCAATGATGCTCCAATTAGTGCTGCGCAAATGCTAGGGGAAGTGAGTAGGCTTCTTAAACATGGAGGGGTCTATATTTTGATAACTTATGGTGATCCTAAAGTTAGAATGCCTCATTTGAGCCGGCCAATGTACAACTGGAGTACTGTTCTGTGTATCCAACGTAAGTGGAATTGTATGTCCTGTCATTTCAATTCCTCTTTTTCTAGCTGCTCAAATATGTTTAGTTTTATTTGTTCAAATTTCTATAAGTGGTTTTGTTTCCATATTGTGATTCAATTATATTTACTAATTTATGGTGAATTTAGTTATTGCATCTCAAGTTGTTTTCTTCTTTTGGTAGCAACTGTTTATATTTTGTTTGTTTCTATTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltransf_11

Protein Analysis

224

Amino Acids

25.55

Weight (kDa)

8.93

Isoelectric Point (pI)

43.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 324
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 422
AcsI RAATTY 3 cut(s) 259, 538, 599
AfaI GTAC 4 cut(s) 93, 148, 431, 442
AgsI TTSAA 4 cut(s) 163, 490, 537, 575
AluBI AGCT 3 cut(s) 65, 168, 507
AluI AGCT 3 cut(s) 65, 168, 507
Alw21I GWGCWC 1 cut(s) 156
Alw44I GTGCAC 1 cut(s) 152
AlwI GGATC 1 cut(s) 386
Ama87I CYCGRG 1 cut(s) 218
AoxI GGCC 1 cut(s) 422
ApaLI GTGCAC 1 cut(s) 152
ApeKI GCWGC 3 cut(s) 37, 320, 507
ApoI RAATTY 3 cut(s) 259, 538, 599
AspLEI GCGC 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 401, 608
AvaI CYCGRG 1 cut(s) 218
BaeGI GKGCMC 1 cut(s) 156
Bbv12I GWGCWC 1 cut(s) 156
BbvI GCAGC 3 cut(s) 24, 307, 494
BccI CCATC 1 cut(s) 118
BciVI GTATCC 1 cut(s) 464
BfaI CTAG 2 cut(s) 332, 504
BfmI CTRYAG 1 cut(s) 46
BfuI GTATCC 1 cut(s) 464
BisI GCNGC 3 cut(s) 38, 321, 508
BlsI GCNGC 3 cut(s) 39, 322, 509
BmcAI AGTACT 1 cut(s) 442
BmeT110I CYCGRG 1 cut(s) 218
BmsI GCATC 3 cut(s) 189, 295, 621
BpmI CTGGAG 1 cut(s) 457
BpuEI CTTGAG 1 cut(s) 601
Bsa29I ATCGAT 1 cut(s) 70
BsaBI GATNNNNATC 1 cut(s) 76
BsaJI CCNNGG 1 cut(s) 174
Bse118I RCCGGY 1 cut(s) 420
Bse1I ACTGG 1 cut(s) 440
Bse3DI GCAATG 1 cut(s) 307
Bse8I GATNNNNATC 1 cut(s) 76
BseCI ATCGAT 1 cut(s) 70
BseDI CCNNGG 1 cut(s) 174
BseJI GATNNNNATC 1 cut(s) 76
BseMI GCAATG 1 cut(s) 307
BseMII CTCAG 1 cut(s) 123
BseNI ACTGG 1 cut(s) 440
BseRI GAGGAG 1 cut(s) 86
BseSI GKGCMC 1 cut(s) 156
BseXI GCAGC 3 cut(s) 24, 307, 494
BsgI GTGCAG 2 cut(s) 23, 290
BshFI GGCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 70
BsiHKAI GWGCWC 1 cut(s) 156
BsiHKCI CYCGRG 1 cut(s) 218
BsiSI CCGG 1 cut(s) 421
BslFI GGGAC 1 cut(s) 100
BsmFI GGGAC 1 cut(s) 100
BsmI GAATGC 1 cut(s) 411
BsnI GGCC 1 cut(s) 424
BsoBI CYCGRG 1 cut(s) 218
Bsp1286I GDGCHC 1 cut(s) 156
Bsp1407I TGTACA 1 cut(s) 429
Bsp143I GATC 1 cut(s) 391
Bsp19I CCATGG 1 cut(s) 174
BspANI GGCC 1 cut(s) 424
BspCNI CTCAG 1 cut(s) 124
BspDI ATCGAT 1 cut(s) 70
BspPI GGATC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 307
BsrFI RCCGGY 1 cut(s) 420
BsrGI TGTACA 1 cut(s) 429
BsrI ACTGG 1 cut(s) 440
BssAI RCCGGY 1 cut(s) 420
BssECI CCNNGG 1 cut(s) 174
BssMI GATC 1 cut(s) 391
BssT1I CCWWGG 1 cut(s) 174
Bst4CI ACNGT 4 cut(s) 30, 151, 445, 646
BstAUI TGTACA 1 cut(s) 429
BstC8I GCNNGC 2 cut(s) 21, 422
BstDEI CTNAG 1 cut(s) 132
BstDSI CCRYGG 1 cut(s) 174
BstHHI GCGC 1 cut(s) 325
BstKTI GATC 1 cut(s) 394
BstMBI GATC 1 cut(s) 391
BstMWI GCNNNNNNNGC 1 cut(s) 283
BstSFI CTRYAG 1 cut(s) 46
BstSLI GKGCMC 1 cut(s) 156
BstV1I GCAGC 3 cut(s) 24, 307, 494
Bsu15I ATCGAT 1 cut(s) 70
BsuI GTATCC 1 cut(s) 464
BsuRI GGCC 1 cut(s) 424
BsuTUI ATCGAT 1 cut(s) 70
BtgI CCRYGG 1 cut(s) 174
BtsIMutI CAGTG 1 cut(s) 26
Cac8I GCNNGC 2 cut(s) 21, 422
CfoI GCGC 1 cut(s) 325
Cfr10I RCCGGY 1 cut(s) 420
ClaI ATCGAT 1 cut(s) 70
CseI GACGC 1 cut(s) 292
Csp6I GTAC 4 cut(s) 92, 147, 430, 441
CspCI CAANNNNNGTGG 2 cut(s) 446, 481
CviAII CATG 2 cut(s) 175, 359
CviJI RGCY 9 cut(s) 23, 65, 109, 168, 236, 349, 420, 424, 507
CviKI_1 RGCY 9 cut(s) 23, 65, 109, 168, 236, 349, 420, 424, 507
CviQI GTAC 4 cut(s) 92, 147, 430, 441
DdeI CTNAG 1 cut(s) 132
DpnI GATC 1 cut(s) 393
DpnII GATC 1 cut(s) 391
EaeI YGGCCR 1 cut(s) 422
Eco130I CCWWGG 1 cut(s) 174
Eco32I GATATC 1 cut(s) 53
Eco88I CYCGRG 1 cut(s) 218
EcoRV GATATC 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 174
ErhI CCWWGG 1 cut(s) 174
FaeI CATG 2 cut(s) 178, 362
FaqI GGGAC 1 cut(s) 100
FatI CATG 2 cut(s) 174, 358
Fnu4HI GCNGC 3 cut(s) 38, 321, 508
Fsp4HI GCNGC 3 cut(s) 38, 321, 508
FspBI CTAG 2 cut(s) 332, 504
FspI TGCGCA 1 cut(s) 324
GlaI GCGC 1 cut(s) 324
GluI GCNGC 3 cut(s) 38, 321, 508
GsuI CTGGAG 1 cut(s) 457
HaeIII GGCC 1 cut(s) 424
HapII CCGG 1 cut(s) 421
HgaI GACGC 1 cut(s) 292
HhaI GCGC 1 cut(s) 325
Hin1II CATG 2 cut(s) 178, 362
Hin6I GCGC 1 cut(s) 323
HinP1I GCGC 1 cut(s) 323
HindIII AAGCTT 1 cut(s) 166
HinfI GANTC 5 cut(s) 72, 141, 182, 188, 571
HpaII CCGG 1 cut(s) 421
HphI GGTGA 2 cut(s) 401, 608
Hpy166II GTNNAC 1 cut(s) 154
Hpy188I TCNGA 3 cut(s) 133, 187, 198
Hpy188III TCNNGA 3 cut(s) 100, 220, 289
Hpy8I GTNNAC 1 cut(s) 154
Hpy99I CGWCG 1 cut(s) 286
HpyCH4III ACNGT 4 cut(s) 30, 151, 445, 646
HpyCH4IV ACGT 1 cut(s) 460
HpyCH4V TGCA 6 cut(s) 40, 154, 202, 271, 277, 612
HpyF10VI GCNNNNNNNGC 1 cut(s) 283
HpyF3I CTNAG 1 cut(s) 132
HpySE526I ACGT 1 cut(s) 460
Hsp92II CATG 2 cut(s) 178, 362
HspAI GCGC 1 cut(s) 323
KroI GCCGGC 1 cut(s) 420
KroNI GCCGGC 1 cut(s) 422
Kzo9I GATC 1 cut(s) 391
LmnI GCTCC 1 cut(s) 313
LpnPI CCDG 6 cut(s) 58, 75, 249, 421, 434, 493
Lsp1109I GCAGC 3 cut(s) 24, 307, 494
LweI GCATC 3 cut(s) 189, 295, 621
MaeI CTAG 2 cut(s) 332, 504
MaeII ACGT 1 cut(s) 460
MalI GATC 1 cut(s) 393
MboI GATC 1 cut(s) 391
MboII GAAGA 2 cut(s) 183, 620
MhlI GDGCHC 1 cut(s) 156
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 6 cut(s) 34, 107, 133, 356, 420, 505
MroNI GCCGGC 1 cut(s) 420
MseI TTAA 1 cut(s) 354
MspI CCGG 1 cut(s) 421
Mva1269I GAATGC 1 cut(s) 411
MwoI GCNNNNNNNGC 1 cut(s) 283
NaeI GCCGGC 1 cut(s) 422
NcoI CCATGG 1 cut(s) 174
NdeII GATC 1 cut(s) 391
NgoMIV GCCGGC 1 cut(s) 420
NlaIII CATG 2 cut(s) 178, 362
NsbI TGCGCA 1 cut(s) 324
PctI GAATGC 1 cut(s) 411
PdiI GCCGGC 1 cut(s) 422
PfeI GAWTC 5 cut(s) 72, 141, 182, 188, 571
PkrI GCNGC 3 cut(s) 39, 322, 509
RsaI GTAC 4 cut(s) 93, 148, 431, 442
RsaNI GTAC 4 cut(s) 92, 147, 430, 441
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 3 cut(s) 38, 321, 508
Sau3AI GATC 1 cut(s) 391
ScaI AGTACT 1 cut(s) 442
SduI GDGCHC 1 cut(s) 156
SetI ASST 4 cut(s) 67, 170, 463, 509
SfaNI GCATC 3 cut(s) 189, 295, 621
SfcI CTRYAG 1 cut(s) 46
SmlI CTYRAG 1 cut(s) 616
SmoI CTYRAG 1 cut(s) 616
SspMI CTAG 2 cut(s) 332, 504
StyI CCWWGG 1 cut(s) 174
TaaI ACNGT 4 cut(s) 30, 151, 445, 646
TaiI ACGT 1 cut(s) 463
TaqI TCGA 3 cut(s) 55, 70, 101
TatI WGTACW 2 cut(s) 429, 440
TfiI GAWTC 5 cut(s) 72, 141, 182, 188, 571
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 33
TseI GCWGC 3 cut(s) 37, 320, 507
TspRI CASTG 1 cut(s) 33
VneI GTGCAC 1 cut(s) 152
XapI RAATTY 3 cut(s) 259, 538, 599
XcmI CCANNNNNNNNNTGG 1 cut(s) 432
XspI CTAG 2 cut(s) 332, 504
ZrmI AGTACT 1 cut(s) 442
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.