Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome
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| Species | Orthologous Gene IDs |
|---|---|
| malus_domestica | MD06G1065100.v1.1 |
| pyrus_communis | pycom04g07360 |
| rosa_chinensis | RchiOBHm_Chr2g0151271 |
| rosa_laevigata | RLG00000029209 RLG00000029405 |
| rosa_multiflora | Rmu_sc0003498.1_g000002 Rmu_sc0009409.1_g000008 |
| rosa_roxburghii | Rroxscaffold_1G00017800 Rroxscaffold_1G00024970 Rroxscaffold_1G00030460 Rroxscaffold_2G00106410 Rroxscaffold_2G00133320 Rroxscaffold_3G00220170 Rroxscaffold_4G00313270 Rroxscaffold_5G00339850 Rroxscaffold_6G00418020 Rroxscaffold_7G00175880 |
| rosa_rugosa | Rorug01G0017700 Rorug05G0318400 Rorug07G0300800 |
| rosa_samantha | Rh1CG004700 Rh1CG043500 Rh1DG112600 Rh1DG195700 Rh2AG218100 Rh2AG300700 Rh2AG454300 Rh2AG454400 Rh2AG459600 Rh2AG581500 Rh2BG119400 Rh2BG123700 Rh2BG174600 Rh2BG524000 Rh2CG125300 Rh2CG441400 Rh2CG441500 Rh2DG120900 Rh2DG125900 Rh2DG297900 Rh2DG324900 Rh2DG339600 Rh3AG238400 Rh3BG207600 Rh5AG278100 Rh5AG507700 Rh5BG529200 Rh5CG324500 Rh7BG426800 Rh7BG450300 |
| rosa_wichuraiana | Rw5G015030 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 327 |
| AciI | CCGC | 1 cut(s) | 325 |
| AcuI | CTGAAG | 1 cut(s) | 378 |
| AgsI | TTSAA | 2 cut(s) | 43, 283 |
| AluBI | AGCT | 2 cut(s) | 39, 83 |
| AluI | AGCT | 2 cut(s) | 39, 83 |
| Alw26I | GTCTC | 3 cut(s) | 54, 182, 396 |
| ApeKI | GCWGC | 2 cut(s) | 413, 416 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 190, 222 |
| AspA2I | CCTAGG | 1 cut(s) | 437 |
| AsuHPI | GGTGA | 2 cut(s) | 130, 172 |
| AvrII | CCTAGG | 1 cut(s) | 437 |
| BbsI | GAAGAC | 1 cut(s) | 89 |
| BbvCI | CCTCAGC | 1 cut(s) | 376 |
| BbvI | GCAGC | 2 cut(s) | 400, 403 |
| BccI | CCATC | 3 cut(s) | 74, 323, 339 |
| BcoDI | GTCTC | 3 cut(s) | 54, 182, 396 |
| BfaI | CTAG | 1 cut(s) | 438 |
| BfmI | CTRYAG | 1 cut(s) | 6 |
| BisI | GCNGC | 3 cut(s) | 325, 414, 417 |
| BlnI | CCTAGG | 1 cut(s) | 437 |
| BlsI | GCNGC | 3 cut(s) | 326, 415, 418 |
| BmiI | GGNNCC | 1 cut(s) | 323 |
| BmrI | ACTGGG | 1 cut(s) | 327 |
| BmsI | GCATC | 2 cut(s) | 296, 352 |
| BmuI | ACTGGG | 1 cut(s) | 327 |
| BpiI | GAAGAC | 1 cut(s) | 89 |
| Bpu10I | CCTNAGC | 1 cut(s) | 376 |
| BpuEI | CTTGAG | 1 cut(s) | 435 |
| BsaI | GGTCTC | 1 cut(s) | 54 |
| BsaJI | CCNNGG | 2 cut(s) | 246, 437 |
| Bse1I | ACTGG | 1 cut(s) | 322 |
| BseDI | CCNNGG | 2 cut(s) | 246, 437 |
| BseGI | GGATG | 2 cut(s) | 66, 447 |
| BseMII | CTCAG | 2 cut(s) | 65, 390 |
| BseNI | ACTGG | 1 cut(s) | 322 |
| BseRI | GAGGAG | 3 cut(s) | 96, 181, 364 |
| BseXI | GCAGC | 2 cut(s) | 400, 403 |
| BseYI | CCCAGC | 1 cut(s) | 79 |
| Bsh1236I | CGCG | 1 cut(s) | 327 |
| BsiSI | CCGG | 1 cut(s) | 223 |
| BsmAI | GTCTC | 3 cut(s) | 54, 182, 396 |
| BsmBI | CGTCTC | 1 cut(s) | 182 |
| Bso31I | GGTCTC | 1 cut(s) | 54 |
| Bsp19I | CCATGG | 1 cut(s) | 246 |
| BspACI | CCGC | 1 cut(s) | 325 |
| BspCNI | CTCAG | 2 cut(s) | 64, 389 |
| BspFNI | CGCG | 1 cut(s) | 327 |
| BspLI | GGNNCC | 1 cut(s) | 323 |
| BspTNI | GGTCTC | 1 cut(s) | 54 |
| BsrI | ACTGG | 1 cut(s) | 322 |
| BssECI | CCNNGG | 2 cut(s) | 246, 437 |
| BssT1I | CCWWGG | 2 cut(s) | 246, 437 |
| Bst4CI | ACNGT | 2 cut(s) | 32, 277 |
| BstC8I | GCNNGC | 1 cut(s) | 85 |
| BstDEI | CTNAG | 2 cut(s) | 51, 376 |
| BstDSI | CCRYGG | 1 cut(s) | 246 |
| BstF5I | GGATG | 2 cut(s) | 66, 447 |
| BstFNI | CGCG | 1 cut(s) | 327 |
| BstMAI | GTCTC | 3 cut(s) | 54, 182, 396 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 219 |
| BstSFI | CTRYAG | 1 cut(s) | 6 |
| BstUI | CGCG | 1 cut(s) | 327 |
| BstV1I | GCAGC | 2 cut(s) | 400, 403 |
| BstV2I | GAAGAC | 1 cut(s) | 89 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 164 |
| BtgI | CCRYGG | 1 cut(s) | 246 |
| BtgZI | GCGATG | 1 cut(s) | 342 |
| BtsCI | GGATG | 2 cut(s) | 66, 447 |
| Cac8I | GCNNGC | 1 cut(s) | 85 |
| CseI | GACGC | 1 cut(s) | 164 |
| CviAII | CATG | 2 cut(s) | 247, 395 |
| CviJI | RGCY | 7 cut(s) | 5, 39, 83, 222, 251, 324, 476 |
| CviKI_1 | RGCY | 7 cut(s) | 5, 39, 83, 222, 251, 324, 476 |
| DdeI | CTNAG | 2 cut(s) | 51, 376 |
| Eco130I | CCWWGG | 2 cut(s) | 246, 437 |
| Eco31I | GGTCTC | 1 cut(s) | 54 |
| Eco57I | CTGAAG | 1 cut(s) | 378 |
| EcoT14I | CCWWGG | 2 cut(s) | 246, 437 |
| ErhI | CCWWGG | 2 cut(s) | 246, 437 |
| Esp3I | CGTCTC | 1 cut(s) | 182 |
| FaeI | CATG | 2 cut(s) | 250, 398 |
| FaiI | YATR | 5 cut(s) | 63, 248, 263, 396, 425 |
| FatI | CATG | 2 cut(s) | 246, 394 |
| Fnu4HI | GCNGC | 3 cut(s) | 325, 414, 417 |
| FokI | GGATG | 2 cut(s) | 53, 454 |
| Fsp4HI | GCNGC | 3 cut(s) | 325, 414, 417 |
| FspBI | CTAG | 1 cut(s) | 438 |
| GluI | GCNGC | 3 cut(s) | 325, 414, 417 |
| GsaI | CCCAGC | 1 cut(s) | 83 |
| HapII | CCGG | 1 cut(s) | 223 |
| HgaI | GACGC | 1 cut(s) | 164 |
| Hin1II | CATG | 2 cut(s) | 250, 398 |
| HinfI | GANTC | 5 cut(s) | 11, 169, 389, 433, 445 |
| HpaII | CCGG | 1 cut(s) | 223 |
| HphI | GGTGA | 2 cut(s) | 130, 172 |
| Hpy166II | GTNNAC | 1 cut(s) | 28 |
| Hpy188I | TCNGA | 1 cut(s) | 54 |
| Hpy188III | TCNNGA | 1 cut(s) | 452 |
| Hpy8I | GTNNAC | 1 cut(s) | 28 |
| HpyAV | CCTTC | 2 cut(s) | 277, 421 |
| HpyCH4III | ACNGT | 2 cut(s) | 32, 277 |
| HpyCH4V | TGCA | 1 cut(s) | 419 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 219 |
| HpyF3I | CTNAG | 2 cut(s) | 51, 376 |
| Hsp92II | CATG | 2 cut(s) | 250, 398 |
| LmnI | GCTCC | 1 cut(s) | 321 |
| LpnPI | CCDG | 5 cut(s) | 77, 93, 236, 282, 303 |
| Lsp1109I | GCAGC | 2 cut(s) | 400, 403 |
| LweI | GCATC | 2 cut(s) | 296, 352 |
| MaeI | CTAG | 1 cut(s) | 438 |
| MaeIII | GTNAC | 1 cut(s) | 352 |
| MluCI | AATT | 1 cut(s) | 158 |
| MlyI | GAGTC | 3 cut(s) | 20, 398, 442 |
| MmeI | TCCRAC | 1 cut(s) | 210 |
| MseI | TTAA | 1 cut(s) | 471 |
| MspI | CCGG | 1 cut(s) | 223 |
| MvnI | CGCG | 1 cut(s) | 327 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 219 |
| NcoI | CCATGG | 1 cut(s) | 246 |
| NlaIII | CATG | 2 cut(s) | 250, 398 |
| NlaIV | GGNNCC | 1 cut(s) | 323 |
| NmuCI | GTSAC | 1 cut(s) | 352 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 106 |
| PfeI | GAWTC | 2 cut(s) | 169, 445 |
| PkrI | GCNGC | 3 cut(s) | 326, 415, 418 |
| PleI | GAGTC | 3 cut(s) | 19, 397, 441 |
| PpsI | GAGTC | 3 cut(s) | 19, 397, 441 |
| PspFI | CCCAGC | 1 cut(s) | 79 |
| PspN4I | GGNNCC | 1 cut(s) | 323 |
| SaqAI | TTAA | 1 cut(s) | 471 |
| SatI | GCNGC | 3 cut(s) | 325, 414, 417 |
| SchI | GAGTC | 3 cut(s) | 20, 398, 442 |
| SetI | ASST | 9 cut(s) | 41, 85, 143, 185, 203, 234, 312, 374, 386 |
| SfaNI | GCATC | 2 cut(s) | 296, 352 |
| SfcI | CTRYAG | 1 cut(s) | 6 |
| SmlI | CTYRAG | 1 cut(s) | 450 |
| SmoI | CTYRAG | 1 cut(s) | 450 |
| Sse9I | AATT | 1 cut(s) | 158 |
| SsiI | CCGC | 1 cut(s) | 325 |
| SspMI | CTAG | 1 cut(s) | 438 |
| StyI | CCWWGG | 2 cut(s) | 246, 437 |
| TaaI | ACNGT | 2 cut(s) | 32, 277 |
| TasI | AATT | 1 cut(s) | 158 |
| TauI | GCSGC | 1 cut(s) | 327 |
| TfiI | GAWTC | 2 cut(s) | 169, 445 |
| Tru1I | TTAA | 1 cut(s) | 471 |
| Tru9I | TTAA | 1 cut(s) | 471 |
| TseFI | GTSAC | 1 cut(s) | 352 |
| TseI | GCWGC | 2 cut(s) | 413, 416 |
| Tsp45I | GTSAC | 1 cut(s) | 352 |
| TspDTI | ATGAA | 1 cut(s) | 440 |
| XmaJI | CCTAGG | 1 cut(s) | 437 |
| XspI | CTAG | 1 cut(s) | 438 |