pycom04g07360

Chloroplast-localized elongation factor EF-G involved in protein synthesis in plastids. Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A- site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0001543
Physical Location & Seq
Forward (+)
7840339 .. 7842073
1735 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g07360.1

Sequence Viewer

Length: 951 bp
ATGGCTACAGAGTCAGTGAGAGTGTACAGTTTTAGCTTCAATGGAGGTTCTCAGACTCGGCCGGCCATTTCTCCCTCCCCAGCTCGCTTTCTCGGTCTTCGCCATCGACCCTCTTCTTCCCTCACTTCGTCTTCGCTTTCCCACTTCTTTGGGAATGTTCGTATCAGCTCCCACTCCTCAAAGCTCTCGAATTTGCGCCAGCAGAGCCGAAGAAACCTCTCTGTTTTTGCCATGGCCGCAGAGGATGGAAGGCGTGCTGTAGCTTTAGAAGATTATCGCAACATAGGAATTATGGCTCACATAGATGCAGGGAAGACGACTACAACTGAAAGAATCTTGTTTTATACTGGAAGAAACTATAAAATAGGGGAGGTACATGAAGGAACAGCAACTATGGACTGGATGGAGCAGGAACAAGAAAGAGGCATTACCATAACTTCTGCTGCAACTACAACATTTTGGGACAATCACAGGATTAACATCATTGACACTCCTGGCCACGTTGACTTCACCCTTGAAGTGGAGCGGGCTCTAAGGGTGCTGGATGGTGCTATATGCTTGTTTGACAGTGTTGCTGGTGTGGAACCTCAATCTGAAACTGTGTGGAGGCAAGCTGATAGATATGGAGTGCCCAGGATTTGCTTTGTCAATAAAATGGATCGTCTTGGAGCAAACTTTTTCCGAACAAGAGACATGATAGTGACAAATTTGGGTGCCAAACCACTTGTACTTCAAATTCCAGTTGGTGCAGAAGATAATTTTAAAGGAGTTATTGATCTTGTGAAGATGAGAGCTATACTTTGGTCTGGCGAAGAGTTGGGTGCTAAATTTGTTTACGAGGAGATTCCATCTGATCTTCTGGAGCTGGCTCAAGAATACCGGGCAGAGATGATAGAAACCATAGTTGAGTTGGATCGATCAAGCAATGGAAGGCTATCTAGAAGGAGTTGA

Protein Analysis

317

Amino Acids

35.49

Weight (kDa)

6.68

Isoelectric Point (pI)

53.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 91 - 246 5.4e-59 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 713
AccBSI CCGCTC 1 cut(s) 526
AciI CCGC 2 cut(s) 237, 526
AclWI GGATC 2 cut(s) 666, 921
AcoI YGGCCR 4 cut(s) 59, 63, 234, 496
AcsI RAATTY 4 cut(s) 190, 706, 735, 827
AfaI GTAC 3 cut(s) 26, 375, 729
AfiI CCNNNNNNNGG 1 cut(s) 520
AgsI TTSAA 3 cut(s) 40, 518, 734
AjnI CCWGG 2 cut(s) 493, 632
AluBI AGCT 8 cut(s) 36, 83, 168, 184, 263, 614, 794, 865
AluI AGCT 8 cut(s) 36, 83, 168, 184, 263, 614, 794, 865
Alw26I GTCTC 1 cut(s) 684
AlwI GGATC 2 cut(s) 666, 921
AoxI GGCC 4 cut(s) 59, 63, 234, 496
ApeKI GCWGC 1 cut(s) 443
ApoI RAATTY 4 cut(s) 190, 706, 735, 827
AspLEI GCGC 1 cut(s) 198
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 1 cut(s) 502
BaeGI GKGCMC 1 cut(s) 633
BalI TGGCCA 1 cut(s) 498
BanI GGYRCC 1 cut(s) 713
BanII GRGCYC 1 cut(s) 532
BbsI GAAGAC 3 cut(s) 89, 123, 320
BbvI GCAGC 1 cut(s) 430
BccI CCATC 5 cut(s) 111, 239, 397, 539, 856
BciT130I CCWGG 2 cut(s) 495, 634
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 1 cut(s) 684
BfaI CTAG 1 cut(s) 939
BfmI CTRYAG 2 cut(s) 6, 258
BisI GCNGC 2 cut(s) 237, 444
BlsI GCNGC 2 cut(s) 238, 445
Bme1390I CCNGG 3 cut(s) 495, 634, 881
BmiI GGNNCC 2 cut(s) 585, 715
BmrFI CCNGG 3 cut(s) 495, 634, 881
BmsI GCATC 1 cut(s) 295
BpiI GAAGAC 3 cut(s) 89, 123, 320
BpmI CTGGAG 1 cut(s) 881
BpuEI CTTGAG 1 cut(s) 855
BpuMI CCSGG 1 cut(s) 881
Bsa29I ATCGAT 1 cut(s) 916
BsaBI GATNNNNATC 1 cut(s) 479
BsaJI CCNNGG 2 cut(s) 231, 632
Bsc4I CCNNNNNNNGG 1 cut(s) 520
Bse118I RCCGGY 1 cut(s) 61
Bse1I ACTGG 3 cut(s) 352, 404, 740
Bse3DI GCAATG 1 cut(s) 931
Bse8I GATNNNNATC 1 cut(s) 479
BseBI CCWGG 2 cut(s) 495, 634
BseCI ATCGAT 1 cut(s) 916
BseDI CCNNGG 2 cut(s) 231, 632
BseGI GGATG 3 cut(s) 250, 408, 550
BseJI GATNNNNATC 1 cut(s) 479
BseLI CCNNNNNNNGG 1 cut(s) 520
BseMI GCAATG 1 cut(s) 931
BseMII CTCAG 1 cut(s) 65
BseNI ACTGG 3 cut(s) 352, 404, 740
BseRI GAGGAG 2 cut(s) 166, 854
BseSI GKGCMC 1 cut(s) 633
BseX3I CGGCCG 1 cut(s) 59
BseXI GCAGC 1 cut(s) 430
BseYI CCCAGC 1 cut(s) 79
BsgI GTGCAG 1 cut(s) 768
Bsh1285I CGRYCG 1 cut(s) 62
BshFI GGCC 4 cut(s) 61, 65, 236, 498
BshNI GGYRCC 1 cut(s) 713
BshVI ATCGAT 1 cut(s) 916
BsiEI CGRYCG 1 cut(s) 62
BsiSI CCGG 2 cut(s) 62, 880
BslFI GGGAC 1 cut(s) 476
BslI CCNNNNNNNGG 1 cut(s) 520
BsmAI GTCTC 1 cut(s) 684
BsmFI GGGAC 1 cut(s) 476
BsnI GGCC 4 cut(s) 61, 65, 236, 498
Bsp1286I GDGCHC 2 cut(s) 532, 633
Bsp1407I TGTACA 1 cut(s) 24
Bsp143I GATC 5 cut(s) 658, 775, 853, 913, 917
Bsp19I CCATGG 1 cut(s) 231
BspACI CCGC 2 cut(s) 237, 526
BspANI GGCC 4 cut(s) 61, 65, 236, 498
BspCNI CTCAG 1 cut(s) 64
BspDI ATCGAT 1 cut(s) 916
BspLI GGNNCC 2 cut(s) 585, 715
BspPI GGATC 2 cut(s) 666, 921
BspT107I GGYRCC 1 cut(s) 713
BsrBI CCGCTC 1 cut(s) 526
BsrDI GCAATG 1 cut(s) 931
BsrFI RCCGGY 1 cut(s) 61
BsrGI TGTACA 1 cut(s) 24
BsrI ACTGG 3 cut(s) 352, 404, 740
BssAI RCCGGY 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 231, 632
BssMI GATC 5 cut(s) 658, 775, 853, 913, 917
BssT1I CCWWGG 1 cut(s) 231
Bst2UI CCWGG 2 cut(s) 495, 634
Bst4CI ACNGT 3 cut(s) 29, 569, 601
Bst6I CTCTTC 2 cut(s) 118, 807
BstAUI TGTACA 1 cut(s) 24
BstC8I GCNNGC 7 cut(s) 63, 85, 200, 255, 528, 612, 867
BstDEI CTNAG 2 cut(s) 51, 533
BstDSI CCRYGG 1 cut(s) 231
BstF5I GGATG 3 cut(s) 250, 408, 550
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 5 cut(s) 661, 778, 856, 916, 920
BstMAI GTCTC 1 cut(s) 684
BstMBI GATC 5 cut(s) 658, 775, 853, 913, 917
BstMCI CGRYCG 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 204, 236
BstNI CCWGG 2 cut(s) 495, 634
BstSCI CCNGG 3 cut(s) 493, 632, 879
BstSFI CTRYAG 2 cut(s) 6, 258
BstSLI GKGCMC 1 cut(s) 633
BstV1I GCAGC 1 cut(s) 430
BstV2I GAAGAC 3 cut(s) 89, 123, 320
BstXI CCANNNNNNTGG 1 cut(s) 149
BstZI CGGCCG 1 cut(s) 59
Bsu15I ATCGAT 1 cut(s) 916
BsuRI GGCC 4 cut(s) 61, 65, 236, 498
BsuTUI ATCGAT 1 cut(s) 916
BtgI CCRYGG 1 cut(s) 231
BtsCI GGATG 3 cut(s) 250, 408, 550
BtsIMutI CAGTG 2 cut(s) 21, 574
Cac8I GCNNGC 7 cut(s) 63, 85, 200, 255, 528, 612, 867
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 61
ClaI ATCGAT 1 cut(s) 916
Csp6I GTAC 3 cut(s) 25, 374, 728
CviAII CATG 3 cut(s) 232, 377, 694
CviQI GTAC 3 cut(s) 25, 374, 728
DdeI CTNAG 2 cut(s) 51, 533
DpnI GATC 5 cut(s) 660, 777, 855, 915, 919
DpnII GATC 5 cut(s) 658, 775, 853, 913, 917
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 4 cut(s) 59, 63, 234, 496
EagI CGGCCG 1 cut(s) 59
Eam1104I CTCTTC 2 cut(s) 118, 807
EarI CTCTTC 2 cut(s) 118, 807
EclXI CGGCCG 1 cut(s) 59
Eco130I CCWWGG 1 cut(s) 231
Eco24I GRGCYC 1 cut(s) 532
Eco52I CGGCCG 1 cut(s) 59
EcoRII CCWGG 2 cut(s) 493, 632
EcoT14I CCWWGG 1 cut(s) 231
EcoT38I GRGCYC 1 cut(s) 532
ErhI CCWWGG 1 cut(s) 231
FaeI CATG 3 cut(s) 235, 380, 697
FaqI GGGAC 1 cut(s) 476
FatI CATG 3 cut(s) 231, 376, 693
FauI CCCGC 1 cut(s) 519
Fnu4HI GCNGC 2 cut(s) 237, 444
FokI GGATG 3 cut(s) 257, 415, 557
FriOI GRGCYC 1 cut(s) 532
FseI GGCCGGCC 1 cut(s) 65
Fsp4HI GCNGC 2 cut(s) 237, 444
FspBI CTAG 1 cut(s) 939
GlaI GCGC 1 cut(s) 197
GluI GCNGC 2 cut(s) 237, 444
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 881
HaeIII GGCC 4 cut(s) 61, 65, 236, 498
HapII CCGG 2 cut(s) 62, 880
HhaI GCGC 1 cut(s) 198
Hin1II CATG 3 cut(s) 235, 380, 697
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 11, 55, 333, 844
HpaII CCGG 2 cut(s) 62, 880
HphI GGTGA 1 cut(s) 502
Hpy166II GTNNAC 3 cut(s) 25, 505, 835
Hpy188I TCNGA 4 cut(s) 54, 595, 683, 853
Hpy188III TCNNGA 4 cut(s) 187, 860, 872, 939
Hpy8I GTNNAC 3 cut(s) 25, 505, 835
HpyAV CCTTC 4 cut(s) 243, 374, 924, 936
HpyCH4III ACNGT 3 cut(s) 29, 569, 601
HpyCH4IV ACGT 1 cut(s) 501
HpyCH4V TGCA 3 cut(s) 308, 446, 749
HpyF10VI GCNNNNNNNGC 2 cut(s) 204, 236
HpyF3I CTNAG 2 cut(s) 51, 533
HpySE526I ACGT 1 cut(s) 501
Hsp92II CATG 3 cut(s) 235, 380, 697
HspAI GCGC 1 cut(s) 196
KroI GCCGGC 1 cut(s) 61
KroNI GCCGGC 1 cut(s) 63
Kzo9I GATC 5 cut(s) 658, 775, 853, 913, 917
LmnI GCTCC 5 cut(s) 173, 406, 523, 668, 862
Lsp1109I GCAGC 1 cut(s) 430
LweI GCATC 1 cut(s) 295
MaeI CTAG 1 cut(s) 939
MaeII ACGT 1 cut(s) 501
MaeIII GTNAC 1 cut(s) 700
MalI GATC 5 cut(s) 660, 777, 855, 915, 919
MbiI CCGCTC 1 cut(s) 526
MboI GATC 5 cut(s) 658, 775, 853, 913, 917
MhlI GDGCHC 2 cut(s) 532, 633
MlsI TGGCCA 1 cut(s) 498
MluCI AATT 6 cut(s) 190, 288, 706, 735, 757, 827
MluNI TGGCCA 1 cut(s) 498
MlyI GAGTC 2 cut(s) 20, 49
MmeI TCCRAC 1 cut(s) 891
Mox20I TGGCCA 1 cut(s) 498
MroNI GCCGGC 1 cut(s) 61
MscI TGGCCA 1 cut(s) 498
MseI TTAA 2 cut(s) 477, 762
MslI CAYNNNNRTG 2 cut(s) 303, 698
Msp20I TGGCCA 1 cut(s) 498
MspI CCGG 2 cut(s) 62, 880
MspR9I CCNGG 3 cut(s) 495, 634, 881
MvaI CCWGG 2 cut(s) 495, 634
MwoI GCNNNNNNNGC 2 cut(s) 204, 236
NaeI GCCGGC 1 cut(s) 63
NciI CCSGG 1 cut(s) 881
NcoI CCATGG 1 cut(s) 231
NdeII GATC 5 cut(s) 658, 775, 853, 913, 917
NgoMIV GCCGGC 1 cut(s) 61
NlaIII CATG 3 cut(s) 235, 380, 697
NlaIV GGNNCC 2 cut(s) 585, 715
NmeAIII GCCGAG 1 cut(s) 37
NmuCI GTSAC 1 cut(s) 700
PdiI GCCGGC 1 cut(s) 63
PfeI GAWTC 2 cut(s) 333, 844
PkrI GCNGC 2 cut(s) 238, 445
PleI GAGTC 2 cut(s) 19, 49
PpsI GAGTC 2 cut(s) 19, 49
Psp6I CCWGG 2 cut(s) 493, 632
PspFI CCCAGC 1 cut(s) 79
PspGI CCWGG 2 cut(s) 493, 632
PspN4I GGNNCC 2 cut(s) 585, 715
RigI GGCCGGCC 1 cut(s) 65
RsaI GTAC 3 cut(s) 26, 375, 729
RsaNI GTAC 3 cut(s) 25, 374, 728
RseI CAYNNNNRTG 2 cut(s) 303, 698
SaqAI TTAA 2 cut(s) 477, 762
SatI GCNGC 2 cut(s) 237, 444
Sau3AI GATC 5 cut(s) 658, 775, 853, 913, 917
SchI GAGTC 2 cut(s) 20, 49
ScrFI CCNGG 3 cut(s) 495, 634, 881
SduI GDGCHC 2 cut(s) 532, 633
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 2 cut(s) 6, 258
SmiMI CAYNNNNRTG 2 cut(s) 303, 698
SmlI CTYRAG 1 cut(s) 870
SmoI CTYRAG 1 cut(s) 870
Sse9I AATT 6 cut(s) 190, 288, 706, 735, 757, 827
SsiI CCGC 2 cut(s) 237, 526
SspMI CTAG 1 cut(s) 939
StyD4I CCNGG 3 cut(s) 493, 632, 879
StyI CCWWGG 1 cut(s) 231
TaaI ACNGT 3 cut(s) 29, 569, 601
TaiI ACGT 1 cut(s) 504
TaqI TCGA 3 cut(s) 106, 188, 916
TaqII GACCGA 1 cut(s) 83
TasI AATT 6 cut(s) 190, 288, 706, 735, 757, 827
TatI WGTACW 2 cut(s) 24, 727
TauI GCSGC 1 cut(s) 239
TfiI GAWTC 2 cut(s) 333, 844
Tru1I TTAA 2 cut(s) 477, 762
Tru9I TTAA 2 cut(s) 477, 762
TscAI CASTG 2 cut(s) 21, 574
TseFI GTSAC 1 cut(s) 700
TseI GCWGC 1 cut(s) 443
Tsp45I GTSAC 1 cut(s) 700
TspDTI ATGAA 1 cut(s) 393
TspRI CASTG 2 cut(s) 21, 574
XapI RAATTY 4 cut(s) 190, 706, 735, 827
XbaI TCTAGA 1 cut(s) 938
XcmI CCANNNNNNNNNTGG 1 cut(s) 907
XspI CTAG 1 cut(s) 939
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.