MD09G1027400.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001371
Physical Location & Seq
Forward (+)
1702725 .. 1716429
13705 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1027400.v1.1.491

Sequence Viewer

Length: 9024 bp
ATGGCAGCGGAAGCTGATGTTCCGGAATCCATATCCGTCACTACACAAGCGGGAGACGGATATGTGTCGACGGGGAAGTTTGACATAACTGTCGACCAATGTTCCCCATTACAGTTGAAAAAATCCGAAGGCCAGGAGGATCAACATCCTGAACCCGTGAAATGCTTTGTAGAGGGTGACATTGCGTACGTTGAAAGTAAGAACGGAATCTCAGAGTGCGGAAATCCCTTGGAACCACTCTCAGATCCAAAGGTTGGCAAACAAGAGACCTGCGTAGCGGAGAGTTTCCAAGTTGATGAATCAAACCAAGCAGAAACAGGGATTGCAGATAGGGAAAATGGAGAAAAAGGAAGTGAAGAAAAGGAAGCCGTGGAGGCAACGGAGAAAAAGTCTGAACAACTTGAGAGTAATGGCATTGTAAATGCTGAAGCAGTGGAAACATCGAGAAGTAAGATAAACCTTGATGAACATGTTCAAGTAATCTCGGATCACCAGGATTCCGGGGATGAAATTCCAACGGAGAAGTCCTTTGCCATGGTTTATGAGGAAAAGGGTCCCGAAGTAACCTCGGAAGCTAAAGAAAGCACTGAGGACAAGCTTGAAAGTGCATCCGTGTTAGATGACCACGAAGAAAGAGCTGGACTTGGAAAGGAAATCCAAGAGAAGAACATCCTAGATACTCCATTAGCCACACAAACAGAAGATATCATTGAGGAGAGTCCAAAAGAAGTTAAGAACCAATATGAAACCGTTGAGGAAACTCCCGAAGCAAGTGGCACTGCAAGTGAAGCAGTTAAGGAGGATGAAAATGCAGAATGCCAGGTTGAAATTCCGTGCGTGAAACACGAGGAAGATACACTGAAAAAAGGTGAGGCACTGGATACAGGAGAGGATTCAGTGGCGAAGGAGGAAGATAGCGAAGGATTTTCTGCTGTAGAGCACATCAAAACAAATTCAACAGATCGCGAAGAAGAAGGCGAGACTAACTTAAAGGATTTTCTGCTTGCTAGATCAAATTTAAATTCTGTGGAGGACGAAGCAGCAACTTCAAGAGAAAGTTCAGAAACAATCATCACTGCTGAAGATGAGAGACAAGTTGTAAACGTAGAACAAAACCGTGAGGAAGAGGGGAAGGCAGAGAAAGCAATTACTAGTTCTGACACCATTGAAGATGAAACTCCTGATGAACAGAATCTCCCGGCGGCAAGTATCTTGATGCGAACTGTTACAGAGTCTGAGAGTGCTGAAGTAAGTGAGAGCACCATATCGGAGCCAAATGAAAATGTTGAAAGATCCGAAGAAGTTCTTAATGTTTCAACCGTGAAATGTGATGAAGATGATCAGGGAGATCGTACCAACCCAACTAAGGATGACAAAGATGTAGAAACATTTACCGCCAAAGCGGCTGAAGATGATGACTATACTAGTCCAGTCACGATTACAGAAACGAGGGCGACAGTAACACCAAATGATGAAGAAAGTTCACCACAGACCTTGCAGGAATGTGATTCAGGAGAGGAGCTTCAACATTTGCGGGATGTGATGCCTGAGGAAACTAAGACAGAAACACCCTGGGAAAATGCAGAAGAAATTACCTGCACGAAAGAGGAAACAACAACTCAGAACACGCAAGAATCCTTGGACGAAGAAAAGAAAGAAGCGACTGAAAACACAAACAATTTAGACGATGACAATGCAAGGGACAAGGTGCTTGAGACTGCCGGTACAGAACAAGAAACTGGAGAACACGTGGTTAGGGAAATTCATACGCTCGCAGAACCACCTCAAGATTTCGTTGATAAAGAAACAAATGAAGAAACCTCCACGGAATATCAGGCCATTCCGGAACTCTCTGCTCCGTTCGTAGTAGAAGAAACAGCTAAGGAAATCCCAAAGGACGAAGAAACCGATGTCTTGATTCTAAAAGAAGAGAAAAAGGAAGAAGATGCAGAATGTAACGAGCCCGCTGAGGTAAAGAGTATGACACGTGAAGCACTACCTGAAACCACAGAGGACATAACAGAGCCAAACAAGGACCTCAATGCTCCAATTTCAGAAGAAATCAACAACACATTTGTTGAAAATATTTCCTCTCTCGCTGAGGTATCTGAGACGGAGACTGAGAAAAATATTCAGGAGAAAGATGGCCTCGCGGTTAGCAATTTTGACTCCGTGTCAGGTGAAGTGAATCATGTAACAGGTTTGAAGGAAGGTGAAACAGAAAATGAGGAACAGAAAGAAAACATTGATACTGCTCCGGAAGAGAAGGCTCAAGCAACCATCTTAAAAGATGAAACGGCCTCGGAAGTTGAAGATATTGGTGTACAAGCAGAGGAAAATTGTGAATCAAAAGAGAAGTTGGAGGAGCATATTCCAACAGCAGCAGATGAGGGTGAAACATTATCGAAGGAAGCCGAGGCAGAAAAGGAGGAGCAGATAAAATATAGTGATATTGCTGCTGAAGAGAAGGGAGCAAACATCTTAACAGATGAGACACCCTTGGAAGATACCGACGTGCAAGCAGAGAAAAATGTCAAACCAATAGAGAACTTGGAGGAGCAAATCCCAATACAAGCAGATGAAGGAGAAACATTCCTGAAGGAAGCCGAAGCAGAAAGCAAGGATGTGATAAAATATACTGATGGTTCTCATGAAGATGTTGGTGGACAAGGGAAGGAAGTTGTCAATTCTGAGGAGAACTTGGACAAGCAGATTCCAACAGGCGACTGTGAAACAAGCTTGAAAGAAGCCAAAGCAGAAAAGGAGGAGCAGGGCAAAAGTACGGATATTGTTCCGGAAGAGAAGGGTCCGGAAACCATCTTAACAGATGCAACATCCTTGGACGTCGAAGATGTTGAGGTAAAAGCAAAGGACTATGCTGAATCAAAAGAGAACGTGGAGGAGCAGATTCCAATAGCAGCAGATGAAGAAAAACTTACTTATATTTCTCTTGAAGATATTGATGTACAAGCAGAGGAAAATTTCATTACACAAATGAATTTGGAGTACCCGATTCTAACAACCTCAGATGAACTAGAAACAGGCTCGGAAGAAACCAAAGAAGAAATTAAGGCGCAGGTGAAAAGTATTGATATTGTTCCTGAAGAGAAGGGTCTAGAAACCATCTCAACAGCTGAGATACCCTTGGTCGTTGAAGATATAGGTGTAAAATCAGAGGAAAGTTTCAAATCAAAAGAGAATGTGGAGGAGCAGATTCCAACAGCAGCAGATGAAGGTGAAAAATTCTTGAAGGAAGCCGAAGCAGAAAACAAGGAGCAGATCGACGAGGAAAATTTAAATTCACAAGAATCCTCAGACAAAGAAAAGGAAGAAGTGACTGAAATTACACACACAAAAGAAGATGACACTGTGCTTGAGACTACCAGTACGGAGCAAGAAACTGGAGAACATGTGGTGTACGAAATTCATACTGTCGCAAAACCCCCTCAAGATTCCATTGATAAAGAAACAAATGAAGAGAACTCCGCGGAACATCAGGCATGTACTGATGCGGCTGATTTGAGTACAACTGCAGAGGAAAAGAACTTAGAGGAACATGCCATTCAGGAACTCTCTTCTCCGCTGGTAGTAGAAGAAGCAACTAATGAAATCCCGAAGGAAGAAGAAACCTACACCACGCTTACCACGCTTCTAGAAGAAGAGGAAAAGGAAGGGCATGCAACATGTAATGAGGCCACTGAGGAATATGCGGCTGGGATTGAGAAAAACATTCAGGAGAAAGATGGCACCACACCTAGCAATTCTGAATCTGTGTCCGGAGAACTGAGTCATGTAACAATTTTGAAGGAATCTGAAACAAAGATCGAAATGCAGGACAAAGACATGGATACTGTTCCTGAAGAGAGGGGTCGAGCAACTATCTTAGCAAATGAAACGACCTTGGAAGTTGAAGATATTGATGTACAATTAAAGAAGAATTTTGAGTCAAAAGAGAACTTGGAGGAACAATTTCCTATAGCTGCAGATGAAGGTGAAATATTCTTGAAAGAAGCCGAAGCAGAAAACAAGGAGCAGATAAAAAATGCTGATATTGCTCATGAAGATGTTGATGTACAAGCGGAGAAAACTGTCAATTCAGAAGAGGACATAGATAAGCAGATTCCACCAATAGCAGATGAAGGCGAAACAAGCTTGAAAGAAGCTGAAGCAGAAATTAAGGAGCAGGTCGCATATGCTGATATTGTTCCTGAAGATAAGGGTCTAGCAGCCATCTTAACAGATGAGACACCCTTGGAAGCAGAAGATACTGGTGTGCAAGCGGAGGAAAGTTTCGAATCAAAAGAGAAGTTCAAGGAGAAAAATCCAATCCCAACTGATGAAGATGAAACATTCTTGAAGGAAGCCGAAGTAGAAAACAAGGAGCAGATAAAATATAGTGATATTGCTCCTGAAGACAAGGGTCTAGCAACCATCTTAACAGGAGAGGCAAATTCCGACACAAAAGAGTTCTCAGACGAGCAGATTCCAACAGCAGTGGATGAAGGTGAAAGGAAGATAGCACATGGAAATGTAGTTTTGAATGACACACCTGGAGTGCAAATTCTTGAAGAAGCAGAACCGGAAGTGGGAGTCGAAGAGGAGAGTTGCAAGTCAGAAGATCAACCTGAAAAGACTAACACGATAGTTGAAGAGGCCTTTGATGAGCCAAAGATCACAGGCCGAGATTTCAACAAAGAAGAAATCGTAGAAGGTAATGTAGCATGTTTTGCACAAGAATCAATTGAAGAGGATACAGTTAACAAATATCAAGAAAACGAAAATGAGAAGAAAAAATTAAAGGAAGAGGGAAGCTGTATAGAGAGCTTAAAAGAAGGCAATGGTGAGTACATTCGAGAAGATGAAAGTGAAACGTTCAAAGATGACGAAAAGGATGCAGTGAAGTCCGGAGGAGAGATTCTTAAAGAGCCACAAACTGGAGTAGATGAGAGCATCACATGTGAGACAACTACAATTGCTGAAAGGTCTGAAGAAATTGTTAGTACTTCACCGGTGAAATATGAAGAAGACGAGCAGGGAGAAAGTAGCAGTCCGGCTTCAAAGGAGGAGGGTAGCAATGAGGACACGTTTTCTATCAAGGTGGCTGAAGAGGGCAATGGCGATAATGGTGCAGTCACAGTCACAGAAACAAGTGCAAACGTAAATGGTAAAGAAGGTTTACCACAGGCCGTGCAGGAATATGAACCAGTAGAGGGCCATGAAAAATTGCTGGATGAGATGCCTGAAGAAAGTAAGACAGAATCATCGAGCAAAAAGGCCGAAGTAGTTACCTGCACAAAAGAGGAAGAGACAACACCCATGAATGATACAAAAGAAAATGAAACTGAAGTAGAGGAGGTGTTTGAGATAGCAAGTATGGAACAAGAAACAGGAGAACATGCAACACGGGAAATTCATACAGTTGAGGACAACTCTCAAGAGTTCCATGATAAAGAAACGACTGTAGAAAATTTGAACGAACATCAGGTGTCATCTGAAGCAGCAGATTCGAGCACAGATTTTGTTGGAAATAACTCAGATGAACAGGCCAGTAGAGAACTCTCTTCTTCGCTGGTAGGAGACGATACAAGTAACGAAAGCTCAAAGCAAGAAGAAAACTATATTTCGAGTCTGAAAGAAGAGGAAAATGAAGTGAATGTGGAATGTAAAGAGCCGGTTGAGGGAGCAAATACGACACACGAAACAAAATTTGAAACGTTAGAGGACAGTATAGAGCCAAAGGAGGACCTGTATGCTCATATTTCAGAGGAAGAAACCCACACAGAAGAAAGCAGTAAAGTAGTTAATTCGCTGCAGTCTGAGGTTTCTGAGAAAGATGTCTGTACAGATTTGGAGGGAGCTGAAGCAGAAACCAAAGAGCAGGTCAAGGATATTGAAATTGATCATGAAGAGAAGGGTCTAACAACCATCTTAACAGATGGAGCCGCCTTGGAAAATGTTAACTCAGAAGTGTTTCCAGAGAACCAGATTCCAAGAGTAGCAGATGGAGGCGAATGCAACACTGAGCTGGCGGGAGATAAGGCTGCAGAGGAAAGCCTTCAAGTGCGACGCCTTGAGGAAGCAGAACCAAAATTGAGGGTTGAAGATGAGAGTCGTGAATCAGAAGACCATCCCAAGAATAAGAACATGATAGTTGAAGAGCCTCAACTGCTAGAGCAGGTCCTGGATGAGCCAAATAGCGCTGACGGAGATTTCAGTGAAGGACAAACCACAGAAGGCAATGCCGCAATTTGTGCACCAGAAATAATTCAAGAAGAAACTGCTAAGAACTCTCAAGACAGTGACAAAGAGACAGAAAATTTACAGAAAGAGGGCAGTGGTATCGAGAGCGTAAAAGCATACAATACTACTGACTTGTCACGAGAGTTTACTCCGGAAGATGGAAGCAAAAAGTTCGATGATAACAAACAGGATACAGTGAAGTTCGAAGGAGAGATACTTAAAGAGCCAGAGATTGATGGAGTAGGTGAGAGCACCACATATGAAACAAATCAGATCGTTGAAACATCTGAAGAAATTTTGGATGCTTCACCAGTGAAGTATGAAGAAGGTCAACCGGGAGAAGGTAGCAGTCAAGATTCAGCAAAAGCTTCAAAGGAGGAGGATGTTAGTGAAGTCGATGACAGAAATGGTGCAGTCACAGTTACTGAGTCAAGTCCAGTGGTAACATCAAAAGAAGAAGAAGATTCAGCAGAGGCTTCAGAGAAATATGAACCGGAAGAAGAGAAGGTACCGGTCAAAATTAGTGATGTAGCAGATGCGAAAGAGGTTTGCAACTTGGAAGATAAAGCTTCTTTTGCTGAAAAGACAACAGAAGAGACAAGCTTGCAGAAGGAAGGGCATGCAGAGGTTTCTGAATTGTCTCCAGAAAAGCTAGATGTTGATGCTACTGAAGAAGAATTCAAAGAAGCTTCTGAAATAGTTCCTGAATGTGATTCTCGGAGCATTGATGTAGTTTCGAAAGATGAGATCATTACTAGTCAGACACTCGACAAAGGGATATTACAAAAGCAAGTTGAAATACCATCTTGTACATTGCTTACCGAGGAAAAGGAGATCGAAGCTTCTCAACATGAGCATGGAACTCCAACCAAAGACAAGAACATACAAGAAGAGCCCGAAAAGGAAGTGCCAGCTGATGGAAACGTGGGAGATTTATTTGCTGTGAGGTCAGAAGACAAGACACCGTTGCAAAAGGAAGAGTCCGAAGAGCTCAAAGCTTCTGAGTTGGCTCTGGAAAAGCTGGATGCAGGTGATACAGAAAAAGAATTCAAAGAAACTTTTAAGACAATCTCTATACCCGATTCTCAAAGCACTGATCCAGTTTCGAAAGATGAAATTGTTGCTGATCAAACACACTTTGAGGAATCTTATGCTTTGATTCCCAAGGAAGAGGAGCTCAGAACTTATCAAGACGATCGTGAAACTAAAACCCAAGACAAAAGTATAGAGGAGGAGTATGCCAAGGAAGCAGAAGTGCCAGCTGATGAAAATGGCAGAGATTTAGTTGCTGCAACGTTGGCAGAAGAGACACTGTTGCAAAAGGAAGAGCCCAGGGAGCTCAAAGTTTCTGAATTGGCTCCAAAAAATCTGGATGCTGGTGAAACGGAAGAAACCAAAGAACCTTTTGAAAAGGTGTCCAAATCTGATTCTCAAAGCGCTGAAGTTGTTTCGGGAGATGAGACGACGGAGATGAAAATTTCTCAATGTGTGCATGAAGCTACAACCCAAGACAAGAATAAAGAAGATGAGAGCACAGAGAAAAATGAAGTTCCAGATGGAAACACTAGCGGTATATTTTTTGCTGCAACTTCTGAAGAAGAGACACGTTTGCAAATGGAAGAGCCTAGAGAGTTTAAAGCTTGTGGGTTGGCTCCGGAAAAGCTAGATGCTGGTGAAACTGAAGAAGAAATCAAAGAAACTTTGGAAACGATCTCTAAATCTGATTCTCAAAGCATTGATACAGTTTCCAAAGATAATATTGTTGCTGATCAAACTCTTGTTGAGGCATCTTACACTTCACTTCCCGAGGAAAAGGAGTACAAGGCAGGTGAAAGTGAAGTGCCAGCTGCTGAAAATGGGGGAGATTTAATTTCTGCAAGGTCAGTCGAAGAGACAATCTTGAAAAAGGAAGAGCCCGGGGAGCTCAAAGTGTCTGAATTGGCTCCGGAAGAGCTGAATGCTGGCGAAACTGAAGAAGAAATCAAGAAAACTCCTGAAACAGTATCTGAACCTGATTCTCCAAGCACTGACGTGGTTTCGAGAGATGAGGCCATTTCTGATCAGACACTCCATGAAGGAATATCAACAGAGAAACTTCAAATACCATCTTCTATAATGCTTCCTGAAGAAAAGGAGATCAAAGCTTCTGAAGAGCATAGAACTACTTTCTGTGACAAGAATATAGAAGAAAACGCAAGCAATTTGCAAATGCAAGTTGATGAGAACGTAAAAGCTACAAGCTTGTTAAAGGAAGAGCCGAGGCAACTCAAAGCTTCTGATTTTGAGCTTCAACTTAAAGCAGAGGTACCAGGGAAAAGTTCAAATGAAGAGATCAAGGAGGATGAGAAACTTCCAGAAGAGTCGGAGCCATATTATGTCAGAGAAGAGAGCCATGATGAAAACAAAGCAAAAGCTGAAAAACAAGGAGAGCAGATTGATGAAACATCTACGATCAAAACAAATGAACATCAGAACGAGTTGTCTGAGGTAGATGAAAAGGGAATTGCTAGTTACAAGTATGAAAGACAGCTTACATTTGAAGGCCTTAGTATCAAGGAGAATGACCCACGCTTTATCGAGGATAAGACAACACATGACGGCTTTCCAGAAGAAGAGAAGAGAGTTGAGGAGGCAGATTTTGAATTTGGTCTTAAAAATCAGGGAGATGAAATAAACTCTAAGAGCAGCATAGCATCAACCCAAAGGGAAAAGGTCAGATTGCATGAGACCCTCACTTCTTTCTTGCCACTCAAGCTATTTATTGCACACAAAAATTCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

3008

Amino Acids

333.46

Weight (kDa)

4.17

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 3 cut(s) 3067, 7259, 8057
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 1 cut(s) 2847
Acc36I ACCTGC 9 cut(s) 278, 1604, 3067, 4174, 5297, 5869, 6166, 7259, 8057
Acc65I GGTACC 2 cut(s) 6748, 8545
AccB1I GGYRCC 3 cut(s) 3746, 6748, 8545
AccB7I CCANNNNNTGG 3 cut(s) 254, 4934, 7157
AccI GTMKAC 2 cut(s) 68, 93
AccII CGCG 3 cut(s) 966, 2153, 3488
AclI AACGTT 3 cut(s) 4871, 5714, 7535
AclWI GGATC 5 cut(s) 147, 239, 495, 1287, 7331
AcvI CACGTG 2 cut(s) 1750, 1988
AcyI GRCGYC 2 cut(s) 2844, 6067
AfeI AGCGCT 2 cut(s) 6199, 7678
AfiI CCNNNNNNNGG 9 cut(s) 254, 1366, 4586, 4934, 5210, 5678, 6398, 6581, 7157
AflIII ACRYGT 8 cut(s) 469, 1747, 1985, 3409, 3683, 4955, 5082, 7844
AgeI ACCGGT 2 cut(s) 5008, 6751
AhdI GACNNNNNGTC 1 cut(s) 2173
AhlI ACTAGT 3 cut(s) 1151, 1424, 6995
AjiI CACGTC 2 cut(s) 2515, 8272
AjnI CCWGG 8 cut(s) 132, 492, 819, 1571, 4549, 6180, 7571, 8548
AleI CACNNNNGTG 1 cut(s) 8270
AloI GAACNNNNNNTCC 2 cut(s) 1179, 1211
Alw44I GTGCAC 1 cut(s) 6252
AlwI GGATC 5 cut(s) 147, 239, 495, 1287, 7331
AlwNI CAGNNNCTG 5 cut(s) 1235, 6181, 6665, 8090, 8246
Ama87I CYCGRG 2 cut(s) 8045, 8156
Aor51HI AGCGCT 2 cut(s) 6199, 7678
ApaLI GTGCAC 1 cut(s) 6252
ArsI GACNNNNNNTTYG 2 cut(s) 5865, 5897
AsiGI ACCGGT 2 cut(s) 5008, 6751
Asp700I GAANNNNTTC 7 cut(s) 471, 1302, 3969, 5970, 6054, 6264, 6939
Asp718I GGTACC 2 cut(s) 6748, 8545
AspLEI GCGC 3 cut(s) 3076, 6200, 7679
AspS9I GGNCC 6 cut(s) 554, 2035, 2807, 5212, 5743, 6178
AsuC2I CCSGG 5 cut(s) 502, 1199, 6576, 8157, 8158
AsuII TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
AvaI CYCGRG 2 cut(s) 8045, 8156
AvaII GGWCC 5 cut(s) 554, 2035, 2807, 5743, 6178
AxyI CCTNAGG 1 cut(s) 1548
BaeGI GKGCMC 1 cut(s) 6256
BaeI ACNNNNGTAYC 4 cut(s) 2499, 2532, 4257, 4290
BanI GGYRCC 3 cut(s) 3746, 6748, 8545
BanII GRGCYC 8 cut(s) 1965, 7137, 7233, 7419, 7572, 7581, 8157, 8166
BarI GAAGNNNNNNTAC 4 cut(s) 6438, 6470, 6732, 6764
BauI CACGAG 2 cut(s) 845, 6378
BbrPI CACGTG 2 cut(s) 1750, 1988
BbsI GAAGAC 4 cut(s) 4419, 5031, 6129, 7200
BbvCI CCTCAGC 2 cut(s) 1968, 2100
BceAI ACGGC 4 cut(s) 353, 2313, 5171, 8854
BciT130I CCWGG 8 cut(s) 134, 494, 821, 1573, 4551, 6182, 7573, 8550
BciVI GTATCC 4 cut(s) 874, 3841, 4744, 6424
BclI TGATCA 5 cut(s) 1339, 5899, 7366, 8008, 8299
BcnI CCSGG 5 cut(s) 502, 1199, 6576, 8157, 8158
BcuI ACTAGT 3 cut(s) 1151, 1424, 6995
BfmI CTRYAG 7 cut(s) 933, 3531, 3975, 3981, 5460, 5810, 6042
BfoI RGCGCY 2 cut(s) 6201, 7680
BfuAI ACCTGC 9 cut(s) 278, 1604, 3067, 4174, 5297, 5869, 6166, 7259, 8057
BfuI GTATCC 4 cut(s) 874, 3841, 4744, 6424
BglI GCCNNNNNGGC 2 cut(s) 374, 1403
BmcAI AGTACT 1 cut(s) 5002
Bme18I GGWCC 5 cut(s) 554, 2035, 2807, 5743, 6178
BmeRI GACNNNNNGTC 1 cut(s) 2173
BmeT110I CYCGRG 2 cut(s) 8045, 8156
BmgBI CACGTC 2 cut(s) 2515, 8272
BmgT120I GGNCC 6 cut(s) 554, 2035, 2807, 5212, 5743, 6178
BoxI GACNNNNGTC 1 cut(s) 4419
BpiI GAAGAC 4 cut(s) 4419, 5031, 6129, 7200
BplI GAGNNNNNCTC 2 cut(s) 5414, 5446
BpmI CTGGAG 5 cut(s) 1761, 3423, 4572, 4956, 6867
Bpu10I CCTNAGC 3 cut(s) 1881, 1968, 2100
Bpu14I TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
BpuMI CCSGG 5 cut(s) 502, 1199, 6576, 8157, 8158
BsaAI YACGTR 2 cut(s) 1750, 1988
BsaBI GATNNNNATC 1 cut(s) 144
BsaHI GRCGYC 2 cut(s) 2844, 6067
BsaI GGTCTC 2 cut(s) 260, 8960
Bsc4I CCNNNNNNNGG 9 cut(s) 254, 1366, 4586, 4934, 5210, 5678, 6398, 6581, 7157
Bse118I RCCGGY 4 cut(s) 1721, 5008, 5671, 6751
Bse21I CCTNAGG 1 cut(s) 1548
Bse3DI GCAATG 6 cut(s) 180, 4843, 5080, 5119, 6244, 7052
Bse8I GATNNNNATC 1 cut(s) 144
BseBI CCWGG 8 cut(s) 134, 494, 821, 1573, 4551, 6182, 7573, 8550
BseJI GATNNNNATC 1 cut(s) 144
BseLI CCNNNNNNNGG 9 cut(s) 254, 1366, 4586, 4934, 5210, 5678, 6398, 6581, 7157
BseMI GCAATG 6 cut(s) 180, 4843, 5080, 5119, 6244, 7052
BseSI GKGCMC 1 cut(s) 6256
BseYI CCCAGC 1 cut(s) 3713
BsgI GTGCAG 5 cut(s) 1582, 5148, 5210, 5275, 6672
Bsh1236I CGCG 3 cut(s) 966, 2153, 3488
Bsh1285I CGRYCG 1 cut(s) 7438
BshNI GGYRCC 3 cut(s) 3746, 6748, 8545
BshTI ACCGGT 2 cut(s) 5008, 6751
BsiEI CGRYCG 1 cut(s) 7438
BsiHKCI CYCGRG 2 cut(s) 8045, 8156
BsiWI CGTACG 1 cut(s) 186
BslFI GGGAC 2 cut(s) 540, 1715
BslI CCNNNNNNNGG 9 cut(s) 254, 1366, 4586, 4934, 5210, 5678, 6398, 6581, 7157
BsmBI CGTCTC 4 cut(s) 48, 2105, 5571, 7694
BsmFI GGGAC 2 cut(s) 540, 1715
BsmI GAATGC 3 cut(s) 821, 6017, 8203
Bso31I GGTCTC 2 cut(s) 260, 8960
BsoBI CYCGRG 2 cut(s) 8045, 8156
Bsp119I TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
Bsp1407I TGTACA 6 cut(s) 2323, 2965, 3922, 4072, 5840, 7049
Bsp19I CCATGG 1 cut(s) 534
Bsp68I TCGCGA 1 cut(s) 966
BspFNI CGCG 3 cut(s) 966, 2153, 3488
BspHI TCATGA 3 cut(s) 2650, 4057, 5902
BspMAI CTGCAG 4 cut(s) 3535, 3985, 5814, 6046
BspMI ACCTGC 9 cut(s) 278, 1604, 3067, 4174, 5297, 5869, 6166, 7259, 8057
BspPI GGATC 5 cut(s) 147, 239, 495, 1287, 7331
BspQI GCTCTTC 9 cut(s) 6150, 7125, 7221, 7560, 7854, 8145, 8184, 8385, 8487
BspT104I TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
BspT107I GGYRCC 3 cut(s) 3746, 6748, 8545
BspTNI GGTCTC 2 cut(s) 260, 8960
BsrDI GCAATG 6 cut(s) 180, 4843, 5080, 5119, 6244, 7052
BsrFI RCCGGY 4 cut(s) 1721, 5008, 5671, 6751
BsrGI TGTACA 6 cut(s) 2323, 2965, 3922, 4072, 5840, 7049
BssAI RCCGGY 4 cut(s) 1721, 5008, 5671, 6751
BssNI GRCGYC 2 cut(s) 2844, 6067
BssSI CACGAG 2 cut(s) 845, 6378
Bst2BI CACGAG 2 cut(s) 845, 6378
Bst2UI CCWGG 8 cut(s) 134, 494, 821, 1573, 4551, 6182, 7573, 8550
BstACI GRCGYC 2 cut(s) 2844, 6067
BstAPI GCANNNNNTGC 2 cut(s) 4727, 6251
BstAUI TGTACA 6 cut(s) 2323, 2965, 3922, 4072, 5840, 7049
BstBAI YACGTR 2 cut(s) 1750, 1988
BstBI TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
BstDSI CCRYGG 4 cut(s) 369, 534, 1824, 3486
BstFNI CGCG 3 cut(s) 966, 2153, 3488
BstH2I RGCGCY 2 cut(s) 6201, 7680
BstHHI GCGC 3 cut(s) 3076, 6200, 7679
BstMCI CGRYCG 1 cut(s) 7438
BstNI CCWGG 8 cut(s) 134, 494, 821, 1573, 4551, 6182, 7573, 8550
BstPAI GACNNNNGTC 1 cut(s) 4419
BstSFI CTRYAG 7 cut(s) 933, 3531, 3975, 3981, 5460, 5810, 6042
BstSLI GKGCMC 1 cut(s) 6256
BstUI CGCG 3 cut(s) 966, 2153, 3488
BstV2I GAAGAC 4 cut(s) 4419, 5031, 6129, 7200
BstX2I RGATCY 2 cut(s) 244, 1292
BstYI RGATCY 2 cut(s) 244, 1292
Bsu36I CCTNAGG 1 cut(s) 1548
BsuI GTATCC 4 cut(s) 874, 3841, 4744, 6424
BtgI CCRYGG 4 cut(s) 369, 534, 1824, 3486
BtrI CACGTC 2 cut(s) 2515, 8272
BtsI GCAGTG 6 cut(s) 438, 777, 1074, 4500, 4902, 6340
BtuMI TCGCGA 1 cut(s) 966
BveI ACCTGC 9 cut(s) 278, 1604, 3067, 4174, 5297, 5869, 6166, 7259, 8057
CaiI CAGNNNCTG 5 cut(s) 1235, 6181, 6665, 8090, 8246
CciI TCATGA 3 cut(s) 2650, 4057, 5902
CfoI GCGC 3 cut(s) 3076, 6200, 7679
Cfr10I RCCGGY 4 cut(s) 1721, 5008, 5671, 6751
Cfr13I GGNCC 6 cut(s) 554, 2035, 2807, 5212, 5743, 6178
Cfr42I CCGCGG 1 cut(s) 3489
Cfr9I CCCGGG 1 cut(s) 8156
CseI GACGC 1 cut(s) 6075
CspAI ACCGGT 2 cut(s) 5008, 6751
CspCI CAANNNNNGTGG 6 cut(s) 1476, 1511, 4476, 4511, 6660, 6695
DraI TTTAAA 3 cut(s) 1020, 3297, 7876
DrdI GACNNNNNNGTC 1 cut(s) 89
DriI GACNNNNNGTC 1 cut(s) 2173
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1105I GACNNNNNGTC 1 cut(s) 2173
Ecl136II GAGCTC 4 cut(s) 7231, 7417, 7579, 8164
Eco147I AGGCCT 2 cut(s) 4655, 8784
Eco24I GRGCYC 8 cut(s) 1965, 7137, 7233, 7419, 7572, 7581, 8157, 8166
Eco31I GGTCTC 2 cut(s) 260, 8960
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 5 cut(s) 554, 2035, 2807, 5743, 6178
Eco47III AGCGCT 2 cut(s) 6199, 7678
Eco53kI GAGCTC 4 cut(s) 7231, 7417, 7579, 8164
Eco72I CACGTG 2 cut(s) 1750, 1988
Eco81I CCTNAGG 1 cut(s) 1548
Eco88I CYCGRG 2 cut(s) 8045, 8156
EcoICRI GAGCTC 4 cut(s) 7231, 7417, 7579, 8164
EcoO109I RGGNCCY 4 cut(s) 554, 2035, 5743, 6178
EcoRI GAATTC 2 cut(s) 6917, 7286
EcoRII CCWGG 8 cut(s) 132, 492, 819, 1571, 4549, 6180, 7571, 8548
EcoRV GATATC 1 cut(s) 706
EcoT38I GRGCYC 8 cut(s) 1965, 7137, 7233, 7419, 7572, 7581, 8157, 8166
Esp3I CGTCTC 4 cut(s) 48, 2105, 5571, 7694
FalI AAGNNNNNCTT 4 cut(s) 1290, 1322, 2919, 2951
FaqI GGGAC 2 cut(s) 540, 1715
FauI CCCGC 4 cut(s) 43, 1527, 1972, 6022
FauNDI CATATG 2 cut(s) 4192, 6499
FbaI TGATCA 5 cut(s) 1339, 5899, 7366, 8008, 8299
FblI GTMKAC 2 cut(s) 68, 93
FriOI GRGCYC 8 cut(s) 1965, 7137, 7233, 7419, 7572, 7581, 8157, 8166
GlaI GCGC 3 cut(s) 3075, 6199, 7678
GsaI CCCAGC 1 cut(s) 3717
GsuI CTGGAG 5 cut(s) 1761, 3423, 4572, 4956, 6867
HaeII RGCGCY 2 cut(s) 6201, 7680
HgaI GACGC 1 cut(s) 6075
HhaI GCGC 3 cut(s) 3076, 6200, 7679
Hin1I GRCGYC 2 cut(s) 2844, 6067
Hin6I GCGC 3 cut(s) 3074, 6198, 7677
HinP1I GCGC 3 cut(s) 3074, 6198, 7677
HincII GTYRAC 5 cut(s) 69, 94, 4759, 5959, 6572
HindII GTYRAC 5 cut(s) 69, 94, 4759, 5959, 6572
HpaI GTTAAC 2 cut(s) 4759, 5959
Hpy99I CGWCG 6 cut(s) 73, 2516, 2849, 3287, 6069, 7708
Hsp92I GRCGYC 2 cut(s) 2844, 6067
HspAI GCGC 3 cut(s) 3074, 6198, 7677
KflI GGGWCCC 1 cut(s) 554
KpnI GGTACC 2 cut(s) 6752, 8549
Ksp22I TGATCA 5 cut(s) 1339, 5899, 7366, 8008, 8299
KspAI GTTAAC 2 cut(s) 4759, 5959
KspI CCGCGG 1 cut(s) 3489
LguI GCTCTTC 9 cut(s) 6150, 7125, 7221, 7560, 7854, 8145, 8184, 8385, 8487
MfeI CAATTG 2 cut(s) 4740, 4971
MflI RGATCY 2 cut(s) 244, 1292
MroXI GAANNNNTTC 7 cut(s) 471, 1302, 3969, 5970, 6054, 6264, 6939
MslI CAYNNNNRTG 5 cut(s) 2655, 4062, 4527, 7095, 8270
MspA1I CMGCKG 8 cut(s) 8, 1967, 3134, 3488, 3583, 7154, 7502, 8087
MunI CAATTG 2 cut(s) 4740, 4971
Mva1269I GAATGC 3 cut(s) 821, 6017, 8203
MvaI CCWGG 8 cut(s) 134, 494, 821, 1573, 4551, 6182, 7573, 8550
MvnI CGCG 3 cut(s) 966, 2153, 3488
NciI CCSGG 5 cut(s) 502, 1199, 6576, 8157, 8158
NcoI CCATGG 1 cut(s) 534
NdeI CATATG 2 cut(s) 4192, 6499
NmeAIII GCCGAG 3 cut(s) 2440, 4706, 8523
NruI TCGCGA 1 cut(s) 966
NspV TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
OliI CACNNNNGTG 1 cut(s) 8270
PaeI GCATGC 2 cut(s) 3680, 6863
PagI TCATGA 3 cut(s) 2650, 4057, 5902
PaqCI CACCTGC 3 cut(s) 3067, 7259, 8057
PasI CCCWGGG 2 cut(s) 1572, 7572
PceI AGGCCT 2 cut(s) 4655, 8784
PciI ACATGT 4 cut(s) 469, 3409, 3683, 4955
PciSI GCTCTTC 9 cut(s) 6150, 7125, 7221, 7560, 7854, 8145, 8184, 8385, 8487
PcsI WCGNNNNNNNCGW 1 cut(s) 1905
PctI GAATGC 3 cut(s) 821, 6017, 8203
PdmI GAANNNNTTC 7 cut(s) 471, 1302, 3969, 5970, 6054, 6264, 6939
Pfl23II CGTACG 1 cut(s) 186
PflMI CCANNNNNTGG 3 cut(s) 254, 4934, 7157
PfoI TCCNGGA 1 cut(s) 6180
PinAI ACCGGT 2 cut(s) 5008, 6751
Ple19I CGATCG 1 cut(s) 7438
PmaCI CACGTG 2 cut(s) 1750, 1988
PmlI CACGTG 2 cut(s) 1750, 1988
Ppu21I YACGTR 2 cut(s) 1750, 1988
PpuMI RGGWCCY 4 cut(s) 554, 2035, 5743, 6178
PscI ACATGT 4 cut(s) 469, 3409, 3683, 4955
PshAI GACNNNNGTC 1 cut(s) 4419
Psp124BI GAGCTC 4 cut(s) 7233, 7419, 7581, 8166
Psp1406I AACGTT 3 cut(s) 4871, 5714, 7535
Psp5II RGGWCCY 4 cut(s) 554, 2035, 5743, 6178
Psp6I CCWGG 8 cut(s) 132, 492, 819, 1571, 4549, 6180, 7571, 8548
PspCI CACGTG 2 cut(s) 1750, 1988
PspFI CCCAGC 1 cut(s) 3713
PspGI CCWGG 8 cut(s) 132, 492, 819, 1571, 4549, 6180, 7571, 8548
PspLI CGTACG 1 cut(s) 186
PspPI GGNCC 6 cut(s) 554, 2035, 2807, 5212, 5743, 6178
PspPPI RGGWCCY 4 cut(s) 554, 2035, 5743, 6178
PstI CTGCAG 4 cut(s) 3535, 3985, 5814, 6046
PstNI CAGNNNCTG 5 cut(s) 1235, 6181, 6665, 8090, 8246
PsuI RGATCY 2 cut(s) 244, 1292
PvuI CGATCG 1 cut(s) 7438
PvuII CAGCTG 4 cut(s) 3134, 7154, 7502, 8087
RruI TCGCGA 1 cut(s) 966
RseI CAYNNNNRTG 5 cut(s) 2655, 4062, 4527, 7095, 8270
SacI GAGCTC 4 cut(s) 7233, 7419, 7581, 8166
SacII CCGCGG 1 cut(s) 3489
SalI GTCGAC 2 cut(s) 67, 92
SapI GCTCTTC 9 cut(s) 6150, 7125, 7221, 7560, 7854, 8145, 8184, 8385, 8487
Sau96I GGNCC 6 cut(s) 554, 2035, 2807, 5212, 5743, 6178
ScaI AGTACT 1 cut(s) 5002
SfcI CTRYAG 7 cut(s) 933, 3531, 3975, 3981, 5460, 5810, 6042
Sfr303I CCGCGG 1 cut(s) 3489
SfuI TTCGAA 4 cut(s) 4293, 6444, 6977, 7346
SgrAI CRCCGGYG 1 cut(s) 5008
SgrBI CCGCGG 1 cut(s) 3489
SinI GGWCC 5 cut(s) 554, 2035, 2807, 5743, 6178
SmaI CCCGGG 1 cut(s) 8158
SmiI ATTTAAAT 2 cut(s) 1020, 3297
SmiMI CAYNNNNRTG 5 cut(s) 2655, 4062, 4527, 7095, 8270
SpeI ACTAGT 3 cut(s) 1151, 1424, 6995
SphI GCATGC 2 cut(s) 3680, 6863
SseBI AGGCCT 2 cut(s) 4655, 8784
SspI AATATT 4 cut(s) 2086, 2131, 3999, 7999
SstI GAGCTC 4 cut(s) 7233, 7419, 7581, 8166
StuI AGGCCT 2 cut(s) 4655, 8784
SwaI ATTTAAAT 2 cut(s) 1020, 3297
TauI GCSGC 6 cut(s) 1205, 1406, 3515, 3713, 5945, 6245
TspMI CCCGGG 1 cut(s) 8156
Van91I CCANNNNNTGG 3 cut(s) 254, 4934, 7157
VneI GTGCAC 1 cut(s) 6252
VpaK11BI GGWCC 5 cut(s) 554, 2035, 2807, 5743, 6178
XbaI TCTAGA 2 cut(s) 3115, 3652
XmaI CCCGGG 1 cut(s) 8156
XmiI GTMKAC 2 cut(s) 68, 93
XmnI GAANNNNTTC 7 cut(s) 471, 1302, 3969, 5970, 6054, 6264, 6939
ZraI GACGTC 1 cut(s) 2845
ZrmI AGTACT 1 cut(s) 5002
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.