Rorug02G0562200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001371
Physical Location & Seq
Forward (+)
68749031 .. 68749472
442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0562200.1

Sequence Viewer

Length: 351 bp
ATGGCTGCGAAAGACGAGCTCGCCGAGTCTCTCAATGACCTCTTCACCAGCGTCTCCACCATGGTCAAATCGGAGCTCCAGGGAACAACCAATCACATGGTGCTCTTAGAGAAGATGAATCTGAGAGTGGCCGAGGAATACAAAGGCTTCGGCGACGTGGCCTCCGGGCTAAGCGTTTTCGTGGAGCAGCTCAAGTCCAAAGCCGGCAGCTTCGACGAGTATGTGAAGCAGATTGATGTCATAGACCAGCAAGTCACCGAATTCGAAGCTGTGGTTTCGGTGCTTGATCGATATGTGGCCATGTTAGAGTCCAAAGTCCAATCCGTGTATCAAAATCCATCGTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.85

Weight (kDa)

4.51

Isoelectric Point (pI)

34.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BLOC1_2 PF10046 13 - 106 4e-29 Biogenesis of lysosome-related organelles complex-1 subunit 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 251
AcoI YGGCCR 2 cut(s) 129, 297
AcsI RAATTY 1 cut(s) 260
AdeI CACNNNGTG 1 cut(s) 100
AjiI CACGTC 1 cut(s) 157
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 5 cut(s) 19, 76, 190, 210, 269
AluI AGCT 5 cut(s) 19, 76, 190, 210, 269
Alw21I GWGCWC 3 cut(s) 21, 78, 105
Alw26I GTCTC 2 cut(s) 33, 58
AoxI GGCC 3 cut(s) 129, 159, 297
ApeKI GCWGC 3 cut(s) 5, 187, 207
ApoI RAATTY 1 cut(s) 260
AsuC2I CCSGG 1 cut(s) 166
AsuHPI GGTGA 2 cut(s) 37, 247
AsuII TTCGAA 1 cut(s) 264
BalI TGGCCA 1 cut(s) 299
BanII GRGCYC 2 cut(s) 21, 78
BbsI GAAGAC 1 cut(s) 336
Bbv12I GWGCWC 3 cut(s) 21, 78, 105
BbvI GCAGC 2 cut(s) 199, 219
BccI CCATC 1 cut(s) 346
BciT130I CCWGG 1 cut(s) 80
BcnI CCSGG 1 cut(s) 166
BcoDI GTCTC 2 cut(s) 33, 58
BisI GCNGC 3 cut(s) 6, 188, 208
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 3 cut(s) 7, 189, 209
Bme1390I CCNGG 2 cut(s) 80, 166
BmgBI CACGTC 1 cut(s) 157
BmrFI CCNGG 2 cut(s) 80, 166
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 62
Bpu1102I GCTNAGC 1 cut(s) 170
Bpu14I TTCGAA 1 cut(s) 264
BpuEI CTTGAG 1 cut(s) 176
BpuMI CCSGG 1 cut(s) 166
Bsa29I ATCGAT 1 cut(s) 289
BsaJI CCNNGG 3 cut(s) 60, 79, 132
BsaXI ACNNNNNCTCC 2 cut(s) 146, 176
Bse118I RCCGGY 1 cut(s) 203
BseBI CCWGG 1 cut(s) 80
BseCI ATCGAT 1 cut(s) 289
BseDI CCNNGG 3 cut(s) 60, 79, 132
BseMII CTCAG 1 cut(s) 113
BseXI GCAGC 2 cut(s) 199, 219
BshFI GGCC 3 cut(s) 131, 161, 299
BshVI ATCGAT 1 cut(s) 289
BsiHKAI GWGCWC 3 cut(s) 21, 78, 105
BsiSI CCGG 2 cut(s) 165, 204
BsmAI GTCTC 2 cut(s) 33, 58
BsmBI CGTCTC 1 cut(s) 58
BsnI GGCC 3 cut(s) 131, 161, 299
Bsp119I TTCGAA 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 21, 78, 105
Bsp143I GATC 1 cut(s) 286
Bsp1720I GCTNAGC 1 cut(s) 170
Bsp19I CCATGG 1 cut(s) 60
BspANI GGCC 3 cut(s) 131, 161, 299
BspCNI CTCAG 1 cut(s) 114
BspDI ATCGAT 1 cut(s) 289
BspT104I TTCGAA 1 cut(s) 264
BsrFI RCCGGY 1 cut(s) 203
BssAI RCCGGY 1 cut(s) 203
BssECI CCNNGG 3 cut(s) 60, 79, 132
BssMI GATC 1 cut(s) 286
BssT1I CCWWGG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 80
Bst6I CTCTTC 1 cut(s) 47
BstBI TTCGAA 1 cut(s) 264
BstC8I GCNNGC 2 cut(s) 21, 205
BstDEI CTNAG 4 cut(s) 106, 122, 170, 348
BstDSI CCRYGG 1 cut(s) 60
BstKTI GATC 1 cut(s) 289
BstMAI GTCTC 2 cut(s) 33, 58
BstMBI GATC 1 cut(s) 286
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 2 cut(s) 78, 164
BstV1I GCAGC 2 cut(s) 199, 219
BstV2I GAAGAC 1 cut(s) 336
BstXI CCANNNNNNTGG 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 289
BsuRI GGCC 3 cut(s) 131, 161, 299
BsuTUI ATCGAT 1 cut(s) 289
BtgI CCRYGG 1 cut(s) 60
BtrI CACGTC 1 cut(s) 157
Cac8I GCNNGC 2 cut(s) 21, 205
Cfr10I RCCGGY 1 cut(s) 203
ClaI ATCGAT 1 cut(s) 289
CseI GACGC 1 cut(s) 40
CviAII CATG 3 cut(s) 61, 97, 301
DdeI CTNAG 4 cut(s) 106, 122, 170, 348
DpnI GATC 1 cut(s) 288
DpnII GATC 1 cut(s) 286
DraIII CACNNNGTG 1 cut(s) 100
DrdI GACNNNNNNGTC 1 cut(s) 251
DseDI GACNNNNNNGTC 1 cut(s) 251
EaeI YGGCCR 2 cut(s) 129, 297
Eam1104I CTCTTC 1 cut(s) 47
EarI CTCTTC 1 cut(s) 47
Ecl136II GAGCTC 2 cut(s) 19, 76
Eco130I CCWWGG 1 cut(s) 60
Eco24I GRGCYC 2 cut(s) 21, 78
Eco53kI GAGCTC 2 cut(s) 19, 76
EcoICRI GAGCTC 2 cut(s) 19, 76
EcoRI GAATTC 1 cut(s) 260
EcoRII CCWGG 1 cut(s) 78
EcoT14I CCWWGG 1 cut(s) 60
EcoT38I GRGCYC 2 cut(s) 21, 78
ErhI CCWWGG 1 cut(s) 60
Esp3I CGTCTC 1 cut(s) 58
FaeI CATG 3 cut(s) 64, 100, 304
FaiI YATR 6 cut(s) 62, 98, 222, 242, 294, 302
FatI CATG 3 cut(s) 60, 96, 300
Fnu4HI GCNGC 3 cut(s) 6, 188, 208
FriOI GRGCYC 2 cut(s) 21, 78
Fsp4HI GCNGC 3 cut(s) 6, 188, 208
GluI GCNGC 3 cut(s) 6, 188, 208
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 3 cut(s) 131, 161, 299
HapII CCGG 2 cut(s) 165, 204
HgaI GACGC 1 cut(s) 40
Hin1II CATG 3 cut(s) 64, 100, 304
HinfI GANTC 3 cut(s) 26, 118, 308
HpaII CCGG 2 cut(s) 165, 204
HphI GGTGA 2 cut(s) 37, 247
Hpy188I TCNGA 2 cut(s) 73, 123
Hpy99I CGWCG 2 cut(s) 158, 218
HpyCH4IV ACGT 1 cut(s) 156
HpyF3I CTNAG 4 cut(s) 106, 122, 170, 348
HpySE526I ACGT 1 cut(s) 156
Hsp92II CATG 3 cut(s) 64, 100, 304
KroI GCCGGC 1 cut(s) 203
KroNI GCCGGC 1 cut(s) 205
Kzo9I GATC 1 cut(s) 286
LmnI GCTCC 3 cut(s) 73, 81, 184
LpnPI CCDG 6 cut(s) 61, 65, 92, 178, 217, 260
Lsp1109I GCAGC 2 cut(s) 199, 219
MaeII ACGT 1 cut(s) 156
MaeIII GTNAC 1 cut(s) 253
MalI GATC 1 cut(s) 288
MboI GATC 1 cut(s) 286
MboII GAAGA 3 cut(s) 34, 124, 336
MhlI GDGCHC 3 cut(s) 21, 78, 105
MlsI TGGCCA 1 cut(s) 299
MluCI AATT 1 cut(s) 260
MluNI TGGCCA 1 cut(s) 299
MlyI GAGTC 2 cut(s) 35, 317
MnlI CCTC 3 cut(s) 50, 127, 172
Mox20I TGGCCA 1 cut(s) 299
MroNI GCCGGC 1 cut(s) 203
MscI TGGCCA 1 cut(s) 299
Msp20I TGGCCA 1 cut(s) 299
MspI CCGG 2 cut(s) 165, 204
MspR9I CCNGG 2 cut(s) 80, 166
MvaI CCWGG 1 cut(s) 80
NaeI GCCGGC 1 cut(s) 205
NciI CCSGG 1 cut(s) 166
NcoI CCATGG 1 cut(s) 60
NdeII GATC 1 cut(s) 286
NgoMIV GCCGGC 1 cut(s) 203
NlaIII CATG 3 cut(s) 64, 100, 304
NmeAIII GCCGAG 2 cut(s) 49, 157
NmuCI GTSAC 1 cut(s) 253
NspV TTCGAA 1 cut(s) 264
PcsI WCGNNNNNNNCGW 1 cut(s) 21
PdiI GCCGGC 1 cut(s) 205
PfeI GAWTC 1 cut(s) 118
PkrI GCNGC 3 cut(s) 7, 189, 209
PleI GAGTC 2 cut(s) 34, 316
PpsI GAGTC 2 cut(s) 34, 316
Psp124BI GAGCTC 2 cut(s) 21, 78
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
SacI GAGCTC 2 cut(s) 21, 78
SatI GCNGC 3 cut(s) 6, 188, 208
Sau3AI GATC 1 cut(s) 286
SchI GAGTC 2 cut(s) 35, 317
ScrFI CCNGG 2 cut(s) 80, 166
SduI GDGCHC 3 cut(s) 21, 78, 105
SetI ASST 7 cut(s) 21, 42, 78, 159, 192, 212, 271
SfuI TTCGAA 1 cut(s) 264
SmlI CTYRAG 1 cut(s) 191
SmoI CTYRAG 1 cut(s) 191
Sse9I AATT 1 cut(s) 260
SstI GAGCTC 2 cut(s) 21, 78
StyD4I CCNGG 2 cut(s) 78, 164
StyI CCWWGG 1 cut(s) 60
TaiI ACGT 1 cut(s) 159
TaqI TCGA 3 cut(s) 213, 264, 289
TasI AATT 1 cut(s) 260
TfiI GAWTC 1 cut(s) 118
TseFI GTSAC 1 cut(s) 253
TseI GCWGC 3 cut(s) 5, 187, 207
Tsp45I GTSAC 1 cut(s) 253
TspDTI ATGAA 1 cut(s) 131
TspGWI ACGGA 1 cut(s) 313
XapI RAATTY 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.