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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 4 cut(s) | 4253, 6151, 7993, 11462 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 8344 |
| AatII | GACGTC | 1 cut(s) | 4653 |
| Acc16I | TGCGCA | 1 cut(s) | 7191 |
| Acc65I | GGTACC | 2 cut(s) | 8206, 8941 |
| AccB1I | GGYRCC | 5 cut(s) | 7108, 8206, 8941, 10390, 15016 |
| AccBSI | CCGCTC | 1 cut(s) | 3647 |
| AccII | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| AccIII | TCCGGA | 1 cut(s) | 13984 |
| AciI | CCGC | 6 cut(s) | 3647, 10475, 11927, 13205, 13383, 15581 |
| AclI | AACGTT | 1 cut(s) | 14634 |
| AclWI | GGATC | 9 cut(s) | 150, 1617, 1929, 3402, 11191, 11362, 13118, 14642, 14655 |
| AcoI | YGGCCR | 1 cut(s) | 295 |
| AcvI | CACGTG | 1 cut(s) | 2253 |
| AcyI | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| AdeI | CACNNNGTG | 4 cut(s) | 12113, 12641, 13160, 14999 |
| AflII | CTTAAG | 2 cut(s) | 3994, 4195 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 5401, 14587 |
| AhlI | ACTAGT | 1 cut(s) | 11558 |
| AjiI | CACGTC | 2 cut(s) | 9022, 10471 |
| AjnI | CCWGG | 7 cut(s) | 4011, 10335, 11547, 14422, 14621, 15249, 15357 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 13560, 13592 |
| Alw44I | GTGCAC | 1 cut(s) | 12949 |
| AlwI | GGATC | 9 cut(s) | 150, 1617, 1929, 3402, 11191, 11362, 13118, 14642, 14655 |
| AlwNI | CAGNNNCTG | 6 cut(s) | 389, 2099, 3122, 8918, 10367, 14423 |
| Ama87I | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| Aor13HI | TCCGGA | 1 cut(s) | 13984 |
| ApaI | GGGCCC | 1 cut(s) | 4012 |
| ApaLI | GTGCAC | 1 cut(s) | 12949 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 158, 190 |
| AseI | ATTAAT | 2 cut(s) | 3354, 13671 |
| Asp718I | GGTACC | 2 cut(s) | 8206, 8941 |
| AspLEI | GCGC | 2 cut(s) | 340, 7192 |
| AsuC2I | CCSGG | 3 cut(s) | 10597, 12004, 12278 |
| AsuII | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| AsuNHI | GCTAGC | 1 cut(s) | 9317 |
| AvaI | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| AvaII | GGWCC | 8 cut(s) | 268, 1885, 2681, 4015, 6232, 6380, 15094, 15361 |
| AxyI | CCTNAGG | 1 cut(s) | 14307 |
| BaeGI | GKGCMC | 2 cut(s) | 4012, 12953 |
| BamHI | GGATCC | 1 cut(s) | 14647 |
| BanI | GGYRCC | 5 cut(s) | 7108, 8206, 8941, 10390, 15016 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 327, 359, 682, 714, 6354, 6386 |
| BauI | CACGAG | 4 cut(s) | 4905, 9949, 13424, 14077 |
| BbrPI | CACGTG | 1 cut(s) | 2253 |
| BbvCI | CCTCAGC | 1 cut(s) | 15114 |
| BceAI | ACGGC | 4 cut(s) | 2166, 3027, 6650, 15150 |
| BciT130I | CCWGG | 7 cut(s) | 4013, 10337, 11549, 14424, 14623, 15251, 15359 |
| BciVI | GTATCC | 2 cut(s) | 5647, 8419 |
| BcnI | CCSGG | 3 cut(s) | 10597, 12004, 12278 |
| BcuI | ACTAGT | 1 cut(s) | 11558 |
| BfrI | CTTAAG | 2 cut(s) | 3994, 4195 |
| BfuI | GTATCC | 2 cut(s) | 5647, 8419 |
| BglII | AGATCT | 6 cut(s) | 5297, 6944, 8777, 10226, 13097, 13243 |
| BlpI | GCTNAGC | 1 cut(s) | 11859 |
| Bme18I | GGWCC | 8 cut(s) | 268, 1885, 2681, 4015, 6232, 6380, 15094, 15361 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 5401, 14587 |
| BmeT110I | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| BmgBI | CACGTC | 2 cut(s) | 9022, 10471 |
| BmrI | ACTGGG | 1 cut(s) | 11540 |
| BmtI | GCTAGC | 1 cut(s) | 9321 |
| BmuI | ACTGGG | 1 cut(s) | 11540 |
| BoxI | GACNNNNGTC | 1 cut(s) | 15099 |
| Bpu10I | CCTNAGC | 1 cut(s) | 15114 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 11859 |
| Bpu14I | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| BpuMI | CCSGG | 3 cut(s) | 10597, 12004, 12278 |
| Bsa29I | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| BsaAI | YACGTR | 2 cut(s) | 2253, 11105 |
| BsaBI | GATNNNNATC | 5 cut(s) | 147, 825, 7785, 11067, 14478 |
| BsaHI | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| BsaI | GGTCTC | 1 cut(s) | 12049 |
| BsaWI | WCCGGW | 2 cut(s) | 5716, 13984 |
| Bse118I | RCCGGY | 2 cut(s) | 293, 12530 |
| Bse21I | CCTNAGG | 1 cut(s) | 14307 |
| Bse8I | GATNNNNATC | 5 cut(s) | 147, 825, 7785, 11067, 14478 |
| BseAI | TCCGGA | 1 cut(s) | 13984 |
| BseBI | CCWGG | 7 cut(s) | 4013, 10337, 11549, 14424, 14623, 15251, 15359 |
| BseCI | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| BseJI | GATNNNNATC | 5 cut(s) | 147, 825, 7785, 11067, 14478 |
| BseSI | GKGCMC | 2 cut(s) | 4012, 12953 |
| BseYI | CCCAGC | 1 cut(s) | 5365 |
| Bsh1236I | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1758 |
| BshNI | GGYRCC | 5 cut(s) | 7108, 8206, 8941, 10390, 15016 |
| BshVI | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| BsiEI | CGRYCG | 1 cut(s) | 1758 |
| BsiHKCI | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| BsiSI | CCGG | 7 cut(s) | 294, 5717, 10596, 12003, 12278, 12531, 13985 |
| BslFI | GGGAC | 3 cut(s) | 1740, 8602, 10051 |
| BsmBI | CGTCTC | 7 cut(s) | 1745, 6497, 9012, 10461, 11229, 14591, 15125 |
| BsmFI | GGGAC | 3 cut(s) | 1740, 8602, 10051 |
| BsmI | GAATGC | 8 cut(s) | 997, 4630, 6620, 8453, 9902, 13028, 14604, 15772 |
| Bso31I | GGTCTC | 1 cut(s) | 12049 |
| BsoBI | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| Bsp119I | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| Bsp120I | GGGCCC | 1 cut(s) | 4008 |
| Bsp13I | TCCGGA | 1 cut(s) | 13984 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 11859 |
| Bsp19I | CCATGG | 1 cut(s) | 15090 |
| Bsp68I | TCGCGA | 3 cut(s) | 41, 488, 11589 |
| BspACI | CCGC | 6 cut(s) | 3647, 10475, 11927, 13205, 13383, 15581 |
| BspDI | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| BspEI | TCCGGA | 1 cut(s) | 13984 |
| BspFNI | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| BspHI | TCATGA | 5 cut(s) | 3568, 4372, 7855, 13210, 15010 |
| BspMAI | CTGCAG | 5 cut(s) | 469, 1150, 2038, 5615, 5746 |
| BspOI | GCTAGC | 1 cut(s) | 9321 |
| BspPI | GGATC | 9 cut(s) | 150, 1617, 1929, 3402, 11191, 11362, 13118, 14642, 14655 |
| BspT104I | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| BspT107I | GGYRCC | 5 cut(s) | 7108, 8206, 8941, 10390, 15016 |
| BspTI | CTTAAG | 2 cut(s) | 3994, 4195 |
| BspTNI | GGTCTC | 1 cut(s) | 12049 |
| BsrBI | CCGCTC | 1 cut(s) | 3647 |
| BsrFI | RCCGGY | 2 cut(s) | 293, 12530 |
| BssAI | RCCGGY | 2 cut(s) | 293, 12530 |
| BssNI | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| BssSI | CACGAG | 4 cut(s) | 4905, 9949, 13424, 14077 |
| Bst2BI | CACGAG | 4 cut(s) | 4905, 9949, 13424, 14077 |
| Bst2UI | CCWGG | 7 cut(s) | 4013, 10337, 11549, 14424, 14623, 15251, 15359 |
| BstACI | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| BstAFI | CTTAAG | 2 cut(s) | 3994, 4195 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 907, 1154, 7892 |
| BstBAI | YACGTR | 2 cut(s) | 2253, 11105 |
| BstBI | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| BstDSI | CCRYGG | 3 cut(s) | 2787, 4344, 15090 |
| BstEII | GGTNACC | 3 cut(s) | 2494, 3346, 4339 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 779 |
| BstFNI | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| BstHHI | GCGC | 2 cut(s) | 340, 7192 |
| BstMCI | CGRYCG | 1 cut(s) | 1758 |
| BstNI | CCWGG | 7 cut(s) | 4013, 10337, 11549, 14424, 14623, 15251, 15359 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 15099 |
| BstPI | GGTNACC | 3 cut(s) | 2494, 3346, 4339 |
| BstSLI | GKGCMC | 2 cut(s) | 4012, 12953 |
| BstUI | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 12068 |
| Bsu15I | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| Bsu36I | CCTNAGG | 1 cut(s) | 14307 |
| BsuI | GTATCC | 2 cut(s) | 5647, 8419 |
| BsuTUI | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| BtgI | CCRYGG | 3 cut(s) | 2787, 4344, 15090 |
| BtgZI | GCGATG | 1 cut(s) | 4014 |
| BtrI | CACGTC | 2 cut(s) | 9022, 10471 |
| BtsI | GCAGTG | 4 cut(s) | 5739, 9844, 11772, 13140 |
| BtuMI | TCGCGA | 3 cut(s) | 41, 488, 11589 |
| CaiI | CAGNNNCTG | 6 cut(s) | 389, 2099, 3122, 8918, 10367, 14423 |
| CciI | TCATGA | 5 cut(s) | 3568, 4372, 7855, 13210, 15010 |
| CfoI | GCGC | 2 cut(s) | 340, 7192 |
| Cfr10I | RCCGGY | 2 cut(s) | 293, 12530 |
| ClaI | ATCGAT | 3 cut(s) | 2172, 3429, 13890 |
| CseI | GACGC | 4 cut(s) | 964, 7313, 12008, 12280 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 11403, 11438 |
| DraI | TTTAAA | 1 cut(s) | 2856 |
| DraIII | CACNNNGTG | 4 cut(s) | 12113, 12641, 13160, 14999 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 8344 |
| DriI | GACNNNNNGTC | 2 cut(s) | 5401, 14587 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 8344 |
| EaeI | YGGCCR | 1 cut(s) | 295 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 5401, 14587 |
| Ecl136II | GAGCTC | 8 cut(s) | 2211, 4566, 5149, 7488, 10707, 11965, 13999, 14143 |
| Eco31I | GGTCTC | 1 cut(s) | 12049 |
| Eco32I | GATATC | 4 cut(s) | 2170, 3310, 3427, 12256 |
| Eco47I | GGWCC | 8 cut(s) | 268, 1885, 2681, 4015, 6232, 6380, 15094, 15361 |
| Eco53kI | GAGCTC | 8 cut(s) | 2211, 4566, 5149, 7488, 10707, 11965, 13999, 14143 |
| Eco72I | CACGTG | 1 cut(s) | 2253 |
| Eco81I | CCTNAGG | 1 cut(s) | 14307 |
| Eco88I | CYCGRG | 3 cut(s) | 3658, 5832, 12875 |
| Eco91I | GGTNACC | 3 cut(s) | 2494, 3346, 4339 |
| EcoICRI | GAGCTC | 8 cut(s) | 2211, 4566, 5149, 7488, 10707, 11965, 13999, 14143 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 779 |
| EcoO109I | RGGNCCY | 4 cut(s) | 1885, 4008, 4009, 15361 |
| EcoO65I | GGTNACC | 3 cut(s) | 2494, 3346, 4339 |
| EcoRI | GAATTC | 1 cut(s) | 4287 |
| EcoRII | CCWGG | 7 cut(s) | 4011, 10335, 11547, 14422, 14621, 15249, 15357 |
| EcoRV | GATATC | 4 cut(s) | 2170, 3310, 3427, 12256 |
| EcoT22I | ATGCAT | 6 cut(s) | 1159, 6620, 6859, 8453, 9902, 10789 |
| Esp3I | CGTCTC | 7 cut(s) | 1745, 6497, 9012, 10461, 11229, 14591, 15125 |
| FaqI | GGGAC | 3 cut(s) | 1740, 8602, 10051 |
| FspI | TGCGCA | 1 cut(s) | 7191 |
| GlaI | GCGC | 2 cut(s) | 339, 7191 |
| GsaI | CCCAGC | 1 cut(s) | 5369 |
| HapII | CCGG | 7 cut(s) | 294, 5717, 10596, 12003, 12278, 12531, 13985 |
| HgaI | GACGC | 4 cut(s) | 964, 7313, 12008, 12280 |
| HhaI | GCGC | 2 cut(s) | 340, 7192 |
| Hin1I | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| Hin6I | GCGC | 2 cut(s) | 338, 7190 |
| HinP1I | GCGC | 2 cut(s) | 338, 7190 |
| HincII | GTYRAC | 5 cut(s) | 97, 1705, 1996, 3253, 13725 |
| HindII | GTYRAC | 5 cut(s) | 97, 1705, 1996, 3253, 13725 |
| HpaI | GTTAAC | 1 cut(s) | 13725 |
| HpaII | CCGG | 7 cut(s) | 294, 5717, 10596, 12003, 12278, 12531, 13985 |
| Hpy99I | CGWCG | 1 cut(s) | 6437 |
| Hsp92I | GRCGYC | 3 cut(s) | 956, 4650, 12000 |
| HspAI | GCGC | 2 cut(s) | 338, 7190 |
| Kpn2I | TCCGGA | 1 cut(s) | 13984 |
| KpnI | GGTACC | 2 cut(s) | 8210, 8945 |
| KroI | GCCGGC | 1 cut(s) | 293 |
| KroNI | GCCGGC | 1 cut(s) | 295 |
| KspAI | GTTAAC | 1 cut(s) | 13725 |
| MbiI | CCGCTC | 1 cut(s) | 3647 |
| MfeI | CAATTG | 8 cut(s) | 5928, 8289, 9738, 9969, 11490, 13182, 14610, 14814 |
| Mph1103I | ATGCAT | 6 cut(s) | 1159, 6620, 6859, 8453, 9902, 10789 |
| MroI | TCCGGA | 1 cut(s) | 13984 |
| MroNI | GCCGGC | 1 cut(s) | 293 |
| MslI | CAYNNNNRTG | 4 cut(s) | 12363, 13428, 14265, 15609 |
| MspCI | CTTAAG | 2 cut(s) | 3994, 4195 |
| MspI | CCGG | 7 cut(s) | 294, 5717, 10596, 12003, 12278, 12531, 13985 |
| MunI | CAATTG | 8 cut(s) | 5928, 8289, 9738, 9969, 11490, 13182, 14610, 14814 |
| Mva1269I | GAATGC | 8 cut(s) | 997, 4630, 6620, 8453, 9902, 13028, 14604, 15772 |
| MvaI | CCWGG | 7 cut(s) | 4013, 10337, 11549, 14424, 14623, 15251, 15359 |
| MvnI | CGCG | 4 cut(s) | 41, 488, 11589, 11867 |
| NaeI | GCCGGC | 1 cut(s) | 295 |
| NciI | CCSGG | 3 cut(s) | 10597, 12004, 12278 |
| NcoI | CCATGG | 1 cut(s) | 15090 |
| NgoMIV | GCCGGC | 1 cut(s) | 293 |
| NheI | GCTAGC | 1 cut(s) | 9317 |
| NmeAIII | GCCGAG | 2 cut(s) | 3838, 6517 |
| NruI | TCGCGA | 3 cut(s) | 41, 488, 11589 |
| NsbI | TGCGCA | 1 cut(s) | 7191 |
| NsiI | ATGCAT | 6 cut(s) | 1159, 6620, 6859, 8453, 9902, 10789 |
| NspV | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| PaeI | GCATGC | 1 cut(s) | 3239 |
| PaeR7I | CTCGAG | 1 cut(s) | 5832 |
| PagI | TCATGA | 5 cut(s) | 3568, 4372, 7855, 13210, 15010 |
| PaqCI | CACCTGC | 4 cut(s) | 4253, 6151, 7993, 11462 |
| PasI | CCCWGGG | 1 cut(s) | 14622 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1764, 11217 |
| PctI | GAATGC | 8 cut(s) | 997, 4630, 6620, 8453, 9902, 13028, 14604, 15772 |
| PdiI | GCCGGC | 1 cut(s) | 295 |
| PflFI | GACNNNGTC | 1 cut(s) | 3023 |
| PfoI | TCCNGGA | 3 cut(s) | 10335, 12276, 15357 |
| PmaCI | CACGTG | 1 cut(s) | 2253 |
| PmlI | CACGTG | 1 cut(s) | 2253 |
| Ppu21I | YACGTR | 2 cut(s) | 2253, 11105 |
| PpuMI | RGGWCCY | 2 cut(s) | 1885, 15361 |
| PshAI | GACNNNNGTC | 1 cut(s) | 15099 |
| PshBI | ATTAAT | 2 cut(s) | 3354, 13671 |
| Psp124BI | GAGCTC | 8 cut(s) | 2213, 4568, 5151, 7490, 10709, 11967, 14001, 14145 |
| Psp1406I | AACGTT | 1 cut(s) | 14634 |
| Psp5II | RGGWCCY | 2 cut(s) | 1885, 15361 |
| Psp6I | CCWGG | 7 cut(s) | 4011, 10335, 11547, 14422, 14621, 15249, 15357 |
| PspCI | CACGTG | 1 cut(s) | 2253 |
| PspEI | GGTNACC | 3 cut(s) | 2494, 3346, 4339 |
| PspFI | CCCAGC | 1 cut(s) | 5365 |
| PspGI | CCWGG | 7 cut(s) | 4011, 10335, 11547, 14422, 14621, 15249, 15357 |
| PspOMI | GGGCCC | 1 cut(s) | 4008 |
| PspPPI | RGGWCCY | 2 cut(s) | 1885, 15361 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1143, 1175 |
| PstI | CTGCAG | 5 cut(s) | 469, 1150, 2038, 5615, 5746 |
| PstNI | CAGNNNCTG | 6 cut(s) | 389, 2099, 3122, 8918, 10367, 14423 |
| PsyI | GACNNNGTC | 1 cut(s) | 3023 |
| RruI | TCGCGA | 3 cut(s) | 41, 488, 11589 |
| RseI | CAYNNNNRTG | 4 cut(s) | 12363, 13428, 14265, 15609 |
| SacI | GAGCTC | 8 cut(s) | 2213, 4568, 5151, 7490, 10709, 11967, 14001, 14145 |
| SalI | GTCGAC | 1 cut(s) | 95 |
| Sfr274I | CTCGAG | 1 cut(s) | 5832 |
| SfuI | TTCGAA | 6 cut(s) | 429, 7016, 8849, 10298, 13340, 14492 |
| SinI | GGWCC | 8 cut(s) | 268, 1885, 2681, 4015, 6232, 6380, 15094, 15361 |
| SlaI | CTCGAG | 1 cut(s) | 5832 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 12363, 13428, 14265, 15609 |
| SpeI | ACTAGT | 1 cut(s) | 11558 |
| SphI | GCATGC | 1 cut(s) | 3239 |
| SsiI | CCGC | 6 cut(s) | 3647, 10475, 11927, 13205, 13383, 15581 |
| SspI | AATATT | 4 cut(s) | 7030, 7179, 9012, 10461 |
| SstI | GAGCTC | 8 cut(s) | 2213, 4568, 5151, 7490, 10709, 11967, 14001, 14145 |
| TauI | GCSGC | 1 cut(s) | 10477 |
| Tth111I | GACNNNGTC | 1 cut(s) | 3023 |
| Vha464I | CTTAAG | 2 cut(s) | 3994, 4195 |
| VneI | GTGCAC | 1 cut(s) | 12949 |
| VpaK11BI | GGWCC | 8 cut(s) | 268, 1885, 2681, 4015, 6232, 6380, 15094, 15361 |
| VspI | ATTAAT | 2 cut(s) | 3354, 13671 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 779 |
| XbaI | TCTAGA | 5 cut(s) | 2125, 11665, 13094, 13246, 15704 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 136 |
| XhoI | CTCGAG | 1 cut(s) | 5832 |
| ZraI | GACGTC | 1 cut(s) | 4651 |
| Zsp2I | ATGCAT | 6 cut(s) | 1159, 6620, 6859, 8453, 9902, 10789 |