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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 163, 353 |
| AcuI | CTGAAG | 1 cut(s) | 231 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 364 |
| AgsI | TTSAA | 3 cut(s) | 46, 170, 413 |
| AjnI | CCWGG | 1 cut(s) | 104 |
| AluBI | AGCT | 2 cut(s) | 21, 337 |
| AluI | AGCT | 2 cut(s) | 21, 337 |
| Alw26I | GTCTC | 3 cut(s) | 140, 373, 391 |
| AspLEI | GCGC | 1 cut(s) | 206 |
| AspS9I | GGNCC | 2 cut(s) | 109, 355 |
| AvaII | GGWCC | 2 cut(s) | 109, 355 |
| BauI | CACGAG | 1 cut(s) | 271 |
| BbsI | GAAGAC | 1 cut(s) | 34 |
| BciT130I | CCWGG | 1 cut(s) | 106 |
| BciVI | GTATCC | 1 cut(s) | 387 |
| BcoDI | GTCTC | 3 cut(s) | 140, 373, 391 |
| BfaI | CTAG | 1 cut(s) | 224 |
| BfuI | GTATCC | 1 cut(s) | 387 |
| Bme1390I | CCNGG | 1 cut(s) | 106 |
| Bme18I | GGWCC | 2 cut(s) | 109, 355 |
| BmgT120I | GGNCC | 2 cut(s) | 109, 355 |
| BmiI | GGNNCC | 1 cut(s) | 110 |
| BmrFI | CCNGG | 1 cut(s) | 106 |
| BoxI | GACNNNNGTC | 1 cut(s) | 114 |
| BpiI | GAAGAC | 1 cut(s) | 34 |
| BsaBI | GATNNNNATC | 1 cut(s) | 261 |
| BsaI | GGTCTC | 1 cut(s) | 373 |
| BsaJI | CCNNGG | 1 cut(s) | 105 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 364 |
| Bse3DI | GCAATG | 1 cut(s) | 55 |
| Bse8I | GATNNNNATC | 1 cut(s) | 261 |
| BseBI | CCWGG | 1 cut(s) | 106 |
| BseDI | CCNNGG | 1 cut(s) | 105 |
| BseJI | GATNNNNATC | 1 cut(s) | 261 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 364 |
| BseMI | GCAATG | 1 cut(s) | 55 |
| BslFI | GGGAC | 1 cut(s) | 122 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 364 |
| BsmAI | GTCTC | 3 cut(s) | 140, 373, 391 |
| BsmFI | GGGAC | 1 cut(s) | 122 |
| Bso31I | GGTCTC | 1 cut(s) | 373 |
| BspACI | CCGC | 2 cut(s) | 163, 353 |
| BspLI | GGNNCC | 1 cut(s) | 110 |
| BspTNI | GGTCTC | 1 cut(s) | 373 |
| BsrDI | GCAATG | 1 cut(s) | 55 |
| BssECI | CCNNGG | 1 cut(s) | 105 |
| BssSI | CACGAG | 1 cut(s) | 271 |
| Bst2BI | CACGAG | 1 cut(s) | 271 |
| Bst2UI | CCWGG | 1 cut(s) | 106 |
| Bst4CI | ACNGT | 1 cut(s) | 194 |
| BstDEI | CTNAG | 1 cut(s) | 67 |
| BstHHI | GCGC | 1 cut(s) | 206 |
| BstMAI | GTCTC | 3 cut(s) | 140, 373, 391 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 275 |
| BstNI | CCWGG | 1 cut(s) | 106 |
| BstNSI | RCATGY | 2 cut(s) | 301, 305 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 114 |
| BstSCI | CCNGG | 1 cut(s) | 104 |
| BstV2I | GAAGAC | 1 cut(s) | 34 |
| BsuI | GTATCC | 1 cut(s) | 387 |
| CfoI | GCGC | 1 cut(s) | 206 |
| Cfr13I | GGNCC | 2 cut(s) | 109, 355 |
| CpoI | CGGWCCG | 1 cut(s) | 355 |
| CspI | CGGWCCG | 1 cut(s) | 355 |
| CviAII | CATG | 2 cut(s) | 298, 302 |
| CviJI | RGCY | 3 cut(s) | 21, 75, 337 |
| CviKI_1 | RGCY | 3 cut(s) | 21, 75, 337 |
| DdeI | CTNAG | 1 cut(s) | 67 |
| Eco31I | GGTCTC | 1 cut(s) | 373 |
| Eco47I | GGWCC | 2 cut(s) | 109, 355 |
| Eco57I | CTGAAG | 1 cut(s) | 231 |
| EcoRII | CCWGG | 1 cut(s) | 104 |
| EcoT22I | ATGCAT | 1 cut(s) | 303 |
| FaeI | CATG | 2 cut(s) | 301, 305 |
| FaqI | GGGAC | 1 cut(s) | 122 |
| FatI | CATG | 2 cut(s) | 297, 301 |
| FauNDI | CATATG | 2 cut(s) | 37, 419 |
| FspBI | CTAG | 1 cut(s) | 224 |
| GlaI | GCGC | 1 cut(s) | 205 |
| HhaI | GCGC | 1 cut(s) | 206 |
| Hin1II | CATG | 2 cut(s) | 301, 305 |
| Hin6I | GCGC | 1 cut(s) | 204 |
| HinP1I | GCGC | 1 cut(s) | 204 |
| HinfI | GANTC | 4 cut(s) | 139, 250, 286, 360 |
| Hpy188I | TCNGA | 2 cut(s) | 86, 359 |
| Hpy188III | TCNNGA | 3 cut(s) | 29, 143, 254 |
| HpyAV | CCTTC | 3 cut(s) | 206, 400, 407 |
| HpyCH4III | ACNGT | 1 cut(s) | 194 |
| HpyCH4V | TGCA | 3 cut(s) | 60, 278, 301 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 275 |
| HpyF3I | CTNAG | 1 cut(s) | 67 |
| Hsp92II | CATG | 2 cut(s) | 301, 305 |
| HspAI | GCGC | 1 cut(s) | 204 |
| LpnPI | CCDG | 6 cut(s) | 14, 91, 118, 128, 161, 296 |
| MaeI | CTAG | 1 cut(s) | 224 |
| MaeIII | GTNAC | 1 cut(s) | 287 |
| MboII | GAAGA | 1 cut(s) | 34 |
| MluCI | AATT | 1 cut(s) | 242 |
| MlyI | GAGTC | 2 cut(s) | 295, 369 |
| MnlI | CCTC | 3 cut(s) | 65, 240, 404 |
| Mph1103I | ATGCAT | 1 cut(s) | 303 |
| MseI | TTAA | 2 cut(s) | 77, 261 |
| MslI | CAYNNNNRTG | 1 cut(s) | 302 |
| MspR9I | CCNGG | 1 cut(s) | 106 |
| MvaI | CCWGG | 1 cut(s) | 106 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 275 |
| NdeI | CATATG | 2 cut(s) | 37, 419 |
| NlaIII | CATG | 2 cut(s) | 301, 305 |
| NlaIV | GGNNCC | 1 cut(s) | 110 |
| NmuCI | GTSAC | 1 cut(s) | 287 |
| NsiI | ATGCAT | 1 cut(s) | 303 |
| NspI | RCATGY | 2 cut(s) | 301, 305 |
| PfeI | GAWTC | 2 cut(s) | 139, 250 |
| PleI | GAGTC | 2 cut(s) | 294, 368 |
| PpsI | GAGTC | 2 cut(s) | 294, 368 |
| PshAI | GACNNNNGTC | 1 cut(s) | 114 |
| Psp6I | CCWGG | 1 cut(s) | 104 |
| PspGI | CCWGG | 1 cut(s) | 104 |
| PspN4I | GGNNCC | 1 cut(s) | 110 |
| PspPI | GGNCC | 2 cut(s) | 109, 355 |
| RseI | CAYNNNNRTG | 1 cut(s) | 302 |
| Rsr2I | CGGWCCG | 1 cut(s) | 355 |
| RsrII | CGGWCCG | 1 cut(s) | 355 |
| SaqAI | TTAA | 2 cut(s) | 77, 261 |
| Sau96I | GGNCC | 2 cut(s) | 109, 355 |
| SchI | GAGTC | 2 cut(s) | 295, 369 |
| ScrFI | CCNGG | 1 cut(s) | 106 |
| SetI | ASST | 2 cut(s) | 23, 339 |
| SinI | GGWCC | 2 cut(s) | 109, 355 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 302 |
| Sse9I | AATT | 1 cut(s) | 242 |
| SsiI | CCGC | 2 cut(s) | 163, 353 |
| SspMI | CTAG | 1 cut(s) | 224 |
| StyD4I | CCNGG | 1 cut(s) | 104 |
| TaaI | ACNGT | 1 cut(s) | 194 |
| TaqI | TCGA | 1 cut(s) | 317 |
| TasI | AATT | 1 cut(s) | 242 |
| TfiI | GAWTC | 2 cut(s) | 139, 250 |
| Tru1I | TTAA | 2 cut(s) | 77, 261 |
| Tru9I | TTAA | 2 cut(s) | 77, 261 |
| TseFI | GTSAC | 1 cut(s) | 287 |
| Tsp45I | GTSAC | 1 cut(s) | 287 |
| TspDTI | ATGAA | 3 cut(s) | 138, 146, 203 |
| VpaK11BI | GGWCC | 2 cut(s) | 109, 355 |
| XceI | RCATGY | 2 cut(s) | 301, 305 |
| XspI | CTAG | 1 cut(s) | 224 |
| Zsp2I | ATGCAT | 1 cut(s) | 303 |