Rorug02G0562100

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001371
Physical Location & Seq
Reverse (-)
68748956 .. 68752179
3224 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0562100.1

Sequence Viewer

Length: 1767 bp
ATGGGTGAGGTTCTGAAACCTTCACTTTCCCTTATGTTAATCTCAACTAGTTCTGGGTTTCGGTATCGCCATTCTAGCCAGCCTTTATATTATGATGTGCCTAAAACATGTTCAAGTACACATAACCTTAGCTTAGTTTCCATGTGGGTACCCAGTGTGATTAGAATGAAGGCAAAATCCATGTCTGTGCCAAACGTGGTGCTTGGTGTGCAATCTGGGTTGGTTTGTGAAAATGAAGAAGATTCTTTGGTTGAGCAGATATTGCCTCCATGGGGAGATATAGCAATTGATAAGGACCCGGTCCAGCCGGAGATTGAACCGGGTGTTGCCGTCCGGCCAGAGGTGACTTTAATTTGGAAGGATGAGTTGAATGTAGATAGGGTAAGTTTTTTAGAGGAGGTGGATGAAGAGACATTGTCAAAGCGGATTTTGGTGCTTAGTAGAACTAATAAAATTAGGAGTGCGTTGGAGCTGTTTACTTCCATGGAGTTATCAGGTCTTGTTCCTAGTTTACATGCGTGTAATTCACTGTTATCTTGTCTGTTGAGAAATGGGTTTTTTGATAATGCCTTGAGAGTGTTTGAGTTTATGAAGAGAAAGAAGTTAACTACGGGGCATAGTTATAGCCTGATACTTAAAGCAGTTTCAGATGCTTGTGGCTGCGACTCTGCCGTTGAAATGTTTAGGGCAATGGAAGGGGAGAGTGGAGTGAGGGATGGTTTTGATACTGTTGTCTATAACACTATGATATCGGTTTGTGGTAAAGTGAACAATTGGCGTGAAACTGAGAGATTGTGGAGAGATATCAAGGAGAATGGTCATACTGGAACACAAGTTACTTATTGCCTCTTAGTTAGCATTTTTGTTCGGTGTGGTGAGCATGAGCTAGCCCTTGATGCTTACAATGAGATGATTGAAAACAAGTTTCAACCAGGGAATGATACAATGCAAGCTATAATTGGTGCATGCTCGAAGGATGGAAAGTGGGATCTGGCACTAAACATCTTTCAGAATATGTTGGACAGTGGGCTCAAGCCAAATAGAATTGCATTCAATGCATTGATAAATTCGCTTGGAAAGGCTGCGGAGGTTGAACTAGCTTTTAGGGTATATAATATCATGAAATCTATGGATCATTCACCTGATCCTTATACATGGAAGGCGTTACTCAGTGCACTGTATAGGGCAAATCGACATGATGATGCTATTAAGCTCTTTGAGAGCATCAAGAGAAGTCGGGGCTCTCAATTGAACTTGCACTTGTACAATATGGCTTTAATGTCTTGCTCGAAGCTTGGGCTATGGGATAAATCTTTGCAGCTTTTGTGGCAATTAGAAGCTTCTGAATTGTCAGTTTCAACGGCATCGTATAACCTTGTAATCAGTGCTTGCGAGATGGCAAGGAAGCCTGAAGTGGCATTGCAAGTGTATGAGCACATGGTTCACCAGAAGTGCACCCCAGACACATTTACTCATCTGTCACTAATAAGGAGTTGCATTTGGGGTTCTTTCTGGGAGGAAGTGGAGGAAATCCTGAATCACGCTGCACCAGATGCATCTCTTTATAACGCTGCCATTCAAGGAATGAGCTTTCAAGGCAAAACCGAAGTAGCAAAGAAACTTTACACGAATATGCGTAAGAGTGGCCTCCAACCAGATGGGAAAACACGTGCCGTTATGCTGCAAAACTTACGAAGACCTAGGAAGAAGCAGCCTTCTCGTTACAGAACAAGTAGGAGCAAGCTGTCCTCCAATCGCCGTTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

588

Amino Acids

66.41

Weight (kDa)

8.27

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 242 - 286 7.6e-08 PPR repeat family
PPR_long PF17177 250 - 406 4e-07 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 278 - 325 7.9e-06 PPR repeat family
PPR_3 PF13812 304 - 360 2.6e-07 Pentatricopeptide repeat domain
TPR_24 PF23276 316 - 436 1.1e-09 Fungal tetratrico peptide repeats
PPR_2 PF13041 346 - 394 3.5e-10 PPR repeat family
PPR_2 PF13041 453 - 499 2.2e-08 PPR repeat family
PPR_2 PF13041 517 - 564 3.3e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1566
Acc65I GGTACC 1 cut(s) 148
AccB1I GGYRCC 1 cut(s) 148
AciI CCGC 2 cut(s) 424, 1085
AclWI GGATC 3 cut(s) 996, 1139, 1140
AcoI YGGCCR 1 cut(s) 335
AcsI RAATTY 1 cut(s) 1066
AcuI CTGAAG 1 cut(s) 1431
AcvI CACGTG 1 cut(s) 1670
AfaI GTAC 3 cut(s) 118, 150, 1265
AfiI CCNNNNNNNGG 2 cut(s) 272, 340
AflIII ACRYGT 2 cut(s) 107, 1667
AhlI ACTAGT 1 cut(s) 47
AjnI CCWGG 1 cut(s) 931
Alw21I GWGCWC 3 cut(s) 1177, 1437, 1457
Alw26I GTCTC 1 cut(s) 404
Alw44I GTGCAC 2 cut(s) 1173, 1453
AlwI GGATC 3 cut(s) 996, 1139, 1140
AoxI GGCC 2 cut(s) 335, 1645
ApaLI GTGCAC 2 cut(s) 1173, 1453
ApeKI GCWGC 7 cut(s) 660, 1082, 1318, 1544, 1571, 1681, 1711
ApoI RAATTY 1 cut(s) 1066
ArsI GACNNNNNNTTYG 2 cut(s) 167, 199
Asp718I GGTACC 1 cut(s) 148
AspA2I CCTAGG 1 cut(s) 1700
AspS9I GGNCC 2 cut(s) 295, 301
AsuC2I CCSGG 2 cut(s) 299, 321
AsuHPI GGTGA 5 cut(s) 17, 355, 887, 1131, 1436
AsuNHI GCTAGC 1 cut(s) 886
AvaII GGWCC 2 cut(s) 295, 301
AvrII CCTAGG 1 cut(s) 1700
BaeGI GKGCMC 2 cut(s) 1177, 1457
BaeI ACNNNNGTAYC 2 cut(s) 717, 750
BanI GGYRCC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 1032, 1244
BbrPI CACGTG 1 cut(s) 1670
BbsI GAAGAC 1 cut(s) 1702
Bbv12I GWGCWC 3 cut(s) 1177, 1437, 1457
BbvI GCAGC 7 cut(s) 647, 1069, 1330, 1531, 1558, 1668, 1723
BccI CCATC 4 cut(s) 710, 971, 1390, 1652
BceAI ACGGC 5 cut(s) 314, 656, 1377, 1658, 1743
BcgI CGANNNNNNTGC 2 cut(s) 1700, 1734
BciT130I CCWGG 1 cut(s) 933
BcnI CCSGG 2 cut(s) 299, 321
BcoDI GTCTC 1 cut(s) 404
BcuI ACTAGT 1 cut(s) 47
BfaI CTAG 6 cut(s) 48, 75, 507, 887, 1097, 1701
BisI GCNGC 7 cut(s) 661, 1083, 1319, 1545, 1572, 1682, 1712
BlnI CCTAGG 1 cut(s) 1700
BlsI GCNGC 7 cut(s) 662, 1084, 1320, 1546, 1573, 1683, 1713
Bme1390I CCNGG 3 cut(s) 299, 321, 933
Bme18I GGWCC 2 cut(s) 295, 301
BmgT120I GGNCC 2 cut(s) 295, 301
BmiI GGNNCC 2 cut(s) 150, 297
BmrFI CCNGG 3 cut(s) 299, 321, 933
BmrI ACTGGG 1 cut(s) 147
BmsI GCATC 7 cut(s) 640, 886, 1192, 1233, 1373, 1543, 1565
BmtI GCTAGC 1 cut(s) 890
BmuI ACTGGG 1 cut(s) 147
BpiI GAAGAC 1 cut(s) 1702
Bpu10I CCTNAGC 1 cut(s) 128
BpuEI CTTGAG 2 cut(s) 592, 1016
BpuMI CCSGG 2 cut(s) 299, 321
BsaAI YACGTR 1 cut(s) 1670
BsaJI CCNNGG 4 cut(s) 269, 483, 932, 1700
Bsc4I CCNNNNNNNGG 2 cut(s) 272, 340
Bse1I ACTGG 2 cut(s) 153, 829
Bse3DI GCAATG 2 cut(s) 696, 1418
BseBI CCWGG 1 cut(s) 933
BseDI CCNNGG 4 cut(s) 269, 483, 932, 1700
BseGI GGATG 4 cut(s) 367, 409, 721, 982
BseLI CCNNNNNNNGG 2 cut(s) 272, 340
BseMI GCAATG 2 cut(s) 696, 1418
BseMII CTCAG 2 cut(s) 777, 1183
BseNI ACTGG 2 cut(s) 153, 829
BseRI GAGGAG 1 cut(s) 410
BseSI GKGCMC 2 cut(s) 1177, 1457
BseXI GCAGC 7 cut(s) 647, 1069, 1330, 1531, 1558, 1668, 1723
BsgI GTGCAG 1 cut(s) 1530
BshFI GGCC 2 cut(s) 337, 1647
BshNI GGYRCC 1 cut(s) 148
BsiHKAI GWGCWC 3 cut(s) 1177, 1437, 1457
BsiSI CCGG 4 cut(s) 299, 308, 320, 334
BslI CCNNNNNNNGG 2 cut(s) 272, 340
BsmAI GTCTC 1 cut(s) 404
BsmI GAATGC 1 cut(s) 1049
BsnI GGCC 2 cut(s) 337, 1647
Bsp1286I GDGCHC 5 cut(s) 1032, 1177, 1244, 1437, 1457
Bsp1407I TGTACA 1 cut(s) 1263
Bsp143I GATC 3 cut(s) 988, 1132, 1144
Bsp19I CCATGG 2 cut(s) 269, 483
BspACI CCGC 2 cut(s) 424, 1085
BspANI GGCC 2 cut(s) 337, 1647
BspCNI CTCAG 2 cut(s) 778, 1182
BspHI TCATGA 1 cut(s) 1119
BspLI GGNNCC 2 cut(s) 150, 297
BspOI GCTAGC 1 cut(s) 890
BspPI GGATC 3 cut(s) 996, 1139, 1140
BspT107I GGYRCC 1 cut(s) 148
BsrDI GCAATG 2 cut(s) 696, 1418
BsrGI TGTACA 1 cut(s) 1263
BsrI ACTGG 2 cut(s) 153, 829
BssECI CCNNGG 4 cut(s) 269, 483, 932, 1700
BssMI GATC 3 cut(s) 988, 1132, 1144
BssT1I CCWWGG 3 cut(s) 269, 483, 1700
Bst2UI CCWGG 1 cut(s) 933
Bst4CI ACNGT 4 cut(s) 531, 730, 1025, 1179
Bst6I CTCTTC 2 cut(s) 402, 587
BstAPI GCANNNNNTGC 3 cut(s) 262, 1055, 1553
BstAUI TGTACA 1 cut(s) 1263
BstBAI YACGTR 1 cut(s) 1670
BstC8I GCNNGC 6 cut(s) 80, 888, 951, 967, 1390, 1742
BstDEI CTNAG 6 cut(s) 128, 133, 437, 786, 850, 1169
BstDSI CCRYGG 2 cut(s) 269, 483
BstF5I GGATG 4 cut(s) 367, 409, 721, 982
BstKTI GATC 3 cut(s) 991, 1135, 1147
BstMAI GTCTC 1 cut(s) 404
BstMBI GATC 3 cut(s) 988, 1132, 1144
BstMWI GCNNNNNNNGC 8 cut(s) 75, 208, 262, 896, 1055, 1327, 1553, 1596
BstNI CCWGG 1 cut(s) 933
BstNSI RCATGY 3 cut(s) 111, 518, 969
BstSCI CCNGG 3 cut(s) 297, 319, 931
BstSLI GKGCMC 2 cut(s) 1177, 1457
BstV1I GCAGC 7 cut(s) 647, 1069, 1330, 1531, 1558, 1668, 1723
BstV2I GAAGAC 1 cut(s) 1702
BstX2I RGATCY 1 cut(s) 988
BstXI CCANNNNNNTGG 1 cut(s) 1658
BstYI RGATCY 1 cut(s) 988
BsuRI GGCC 2 cut(s) 337, 1647
BtgI CCRYGG 2 cut(s) 269, 483
BtsCI GGATG 4 cut(s) 367, 409, 721, 982
BtsIMutI CAGTG 6 cut(s) 160, 527, 1030, 1175, 1177, 1390
Cac8I GCNNGC 6 cut(s) 80, 888, 951, 967, 1390, 1742
CciI TCATGA 1 cut(s) 1119
Cfr13I GGNCC 2 cut(s) 295, 301
Csp6I GTAC 3 cut(s) 117, 149, 1264
CviQI GTAC 3 cut(s) 117, 149, 1264
DdeI CTNAG 6 cut(s) 128, 133, 437, 786, 850, 1169
DpnI GATC 3 cut(s) 990, 1134, 1146
DpnII GATC 3 cut(s) 988, 1132, 1144
EaeI YGGCCR 1 cut(s) 335
Eam1104I CTCTTC 2 cut(s) 402, 587
EarI CTCTTC 2 cut(s) 402, 587
Eco130I CCWWGG 3 cut(s) 269, 483, 1700
Eco24I GRGCYC 2 cut(s) 1032, 1244
Eco32I GATATC 2 cut(s) 750, 805
Eco47I GGWCC 2 cut(s) 295, 301
Eco57I CTGAAG 1 cut(s) 1431
Eco72I CACGTG 1 cut(s) 1670
EcoO109I RGGNCCY 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 931
EcoRV GATATC 2 cut(s) 750, 805
EcoT14I CCWWGG 3 cut(s) 269, 483, 1700
EcoT22I ATGCAT 2 cut(s) 1060, 1558
EcoT38I GRGCYC 2 cut(s) 1032, 1244
ErhI CCWWGG 3 cut(s) 269, 483, 1700
Fnu4HI GCNGC 7 cut(s) 661, 1083, 1319, 1545, 1572, 1682, 1712
FokI GGATG 4 cut(s) 374, 416, 728, 989
FriOI GRGCYC 2 cut(s) 1032, 1244
Fsp4HI GCNGC 7 cut(s) 661, 1083, 1319, 1545, 1572, 1682, 1712
FspBI CTAG 6 cut(s) 48, 75, 507, 887, 1097, 1701
GluI GCNGC 7 cut(s) 661, 1083, 1319, 1545, 1572, 1682, 1712
HaeIII GGCC 2 cut(s) 337, 1647
HapII CCGG 4 cut(s) 299, 308, 320, 334
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HindIII AAGCTT 2 cut(s) 1292, 1338
HinfI GANTC 3 cut(s) 242, 665, 1537
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 4 cut(s) 299, 308, 320, 334
HphI GGTGA 5 cut(s) 17, 355, 887, 1131, 1436
Hpy166II GTNNAC 8 cut(s) 119, 477, 512, 606, 769, 1175, 1444, 1455
Hpy188I TCNGA 4 cut(s) 15, 649, 1011, 1345
Hpy188III TCNNGA 3 cut(s) 1120, 1228, 1534
Hpy8I GTNNAC 8 cut(s) 119, 477, 512, 606, 769, 1175, 1444, 1455
HpyAV CCTTC 7 cut(s) 30, 163, 352, 689, 967, 1153, 1725
HpyCH4III ACNGT 4 cut(s) 531, 730, 1025, 1179
HpyCH4IV ACGT 2 cut(s) 195, 1669
HpyF10VI GCNNNNNNNGC 8 cut(s) 75, 208, 262, 896, 1055, 1327, 1553, 1596
HpyF3I CTNAG 6 cut(s) 128, 133, 437, 786, 850, 1169
HpySE526I ACGT 2 cut(s) 195, 1669
KpnI GGTACC 1 cut(s) 152
KspAI GTTAAC 1 cut(s) 606
Kzo9I GATC 3 cut(s) 988, 1132, 1144
LmnI GCTCC 2 cut(s) 469, 1737
Lsp1109I GCAGC 7 cut(s) 647, 1069, 1330, 1531, 1558, 1668, 1723
LweI GCATC 7 cut(s) 640, 886, 1192, 1233, 1373, 1543, 1565
MaeI CTAG 6 cut(s) 48, 75, 507, 887, 1097, 1701
MaeII ACGT 2 cut(s) 195, 1669
MaeIII GTNAC 6 cut(s) 343, 835, 1164, 1479, 1721, 1760
MalI GATC 3 cut(s) 990, 1134, 1146
MboI GATC 3 cut(s) 988, 1132, 1144
MboII GAAGA 6 cut(s) 248, 251, 419, 604, 1707, 1717
MfeI CAATTG 3 cut(s) 285, 772, 1247
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 5 cut(s) 1032, 1177, 1244, 1437, 1457
MlyI GAGTC 1 cut(s) 659
MmeI TCCRAC 3 cut(s) 447, 999, 1675
Mph1103I ATGCAT 2 cut(s) 1060, 1558
MseI TTAA 6 cut(s) 38, 350, 605, 636, 1209, 1277
MslI CAYNNNNRTG 3 cut(s) 185, 1200, 1631
MspI CCGG 4 cut(s) 299, 308, 320, 334
MspR9I CCNGG 3 cut(s) 299, 321, 933
MunI CAATTG 3 cut(s) 285, 772, 1247
Mva1269I GAATGC 1 cut(s) 1049
MvaI CCWGG 1 cut(s) 933
MwoI GCNNNNNNNGC 8 cut(s) 75, 208, 262, 896, 1055, 1327, 1553, 1596
NciI CCSGG 2 cut(s) 299, 321
NcoI CCATGG 2 cut(s) 269, 483
NdeII GATC 3 cut(s) 988, 1132, 1144
NheI GCTAGC 1 cut(s) 886
NlaIV GGNNCC 2 cut(s) 150, 297
NmuCI GTSAC 2 cut(s) 343, 1479
NsiI ATGCAT 2 cut(s) 1060, 1558
NspI RCATGY 3 cut(s) 111, 518, 969
PaeI GCATGC 1 cut(s) 969
PagI TCATGA 1 cut(s) 1119
PciI ACATGT 1 cut(s) 107
PctI GAATGC 1 cut(s) 1049
PfeI GAWTC 2 cut(s) 242, 1537
PflFI GACNNNGTC 2 cut(s) 299, 415
PkrI GCNGC 7 cut(s) 662, 1084, 1320, 1546, 1573, 1683, 1713
PleI GAGTC 1 cut(s) 659
PmaCI CACGTG 1 cut(s) 1670
PmlI CACGTG 1 cut(s) 1670
PpsI GAGTC 1 cut(s) 659
Ppu21I YACGTR 1 cut(s) 1670
PpuMI RGGWCCY 1 cut(s) 295
PscI ACATGT 1 cut(s) 107
PsiI TTATAA 1 cut(s) 1566
Psp5II RGGWCCY 1 cut(s) 295
Psp6I CCWGG 1 cut(s) 931
PspCI CACGTG 1 cut(s) 1670
PspGI CCWGG 1 cut(s) 931
PspN4I GGNNCC 2 cut(s) 150, 297
PspPI GGNCC 2 cut(s) 295, 301
PspPPI RGGWCCY 1 cut(s) 295
PsrI GAACNNNNNNTAC 2 cut(s) 820, 852
PsuI RGATCY 1 cut(s) 988
PsyI GACNNNGTC 2 cut(s) 299, 415
RsaI GTAC 3 cut(s) 118, 150, 1265
RsaNI GTAC 3 cut(s) 117, 149, 1264
RseI CAYNNNNRTG 3 cut(s) 185, 1200, 1631
SaqAI TTAA 6 cut(s) 38, 350, 605, 636, 1209, 1277
SatI GCNGC 7 cut(s) 661, 1083, 1319, 1545, 1572, 1682, 1712
Sau3AI GATC 3 cut(s) 988, 1132, 1144
Sau96I GGNCC 2 cut(s) 295, 301
SchI GAGTC 1 cut(s) 659
ScrFI CCNGG 3 cut(s) 299, 321, 933
SduI GDGCHC 5 cut(s) 1032, 1177, 1244, 1437, 1457
SfaNI GCATC 7 cut(s) 640, 886, 1192, 1233, 1373, 1543, 1565
SinI GGWCC 2 cut(s) 295, 301
SmiMI CAYNNNNRTG 3 cut(s) 185, 1200, 1631
SmlI CTYRAG 2 cut(s) 571, 1031
SmoI CTYRAG 2 cut(s) 571, 1031
SpeI ACTAGT 1 cut(s) 47
SphI GCATGC 1 cut(s) 969
SsiI CCGC 2 cut(s) 424, 1085
SspMI CTAG 6 cut(s) 48, 75, 507, 887, 1097, 1701
StyD4I CCNGG 3 cut(s) 297, 319, 931
StyI CCWWGG 3 cut(s) 269, 483, 1700
TaaI ACNGT 4 cut(s) 531, 730, 1025, 1179
TaiI ACGT 2 cut(s) 198, 1672
TaqI TCGA 3 cut(s) 971, 1192, 1289
TatI WGTACW 2 cut(s) 116, 1263
TfiI GAWTC 2 cut(s) 242, 1537
Tru1I TTAA 6 cut(s) 38, 350, 605, 636, 1209, 1277
Tru9I TTAA 6 cut(s) 38, 350, 605, 636, 1209, 1277
TscAI CASTG 6 cut(s) 160, 534, 1030, 1177, 1182, 1390
TseFI GTSAC 2 cut(s) 343, 1479
TseI GCWGC 7 cut(s) 660, 1082, 1318, 1544, 1571, 1681, 1711
Tsp45I GTSAC 2 cut(s) 343, 1479
TspDTI ATGAA 5 cut(s) 182, 249, 420, 605, 1136
TspRI CASTG 6 cut(s) 160, 534, 1030, 1177, 1182, 1390
Tth111I GACNNNGTC 2 cut(s) 299, 415
VneI GTGCAC 2 cut(s) 1173, 1453
VpaK11BI GGWCC 2 cut(s) 295, 301
XapI RAATTY 1 cut(s) 1066
XceI RCATGY 3 cut(s) 111, 518, 969
XmaJI CCTAGG 1 cut(s) 1700
XspI CTAG 6 cut(s) 48, 75, 507, 887, 1097, 1701
Zsp2I ATGCAT 2 cut(s) 1060, 1558
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.