Rorug02G0562300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0001371
Physical Location & Seq
Forward (+)
68754684 .. 68760590
5907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0562300.1

Sequence Viewer

Length: 2214 bp
ATGACGACCGCCATGGGTCCTCCGCCGCCTCCGCCTCCACTCCCCGCAGACCCCGCCGACTCCGAAACGCTAGAAAATGTGCCGATGGAGACCACCTCCCATTCTTCAACCCAAATGAAGCCCCCGATGGGGCCTCCACCTCCCAAAAACCCTAGTCCACCTCCTCCTCCCCAAAACCCTAACCCCACTCCACCCTCTACATCCACCACCGACTCCGAAACCGCGCCGCCGGAGACATCCAAGCACCAATCCCAAGGCTTTGCCGTCCCTTATTCGATTCCTGAATGGAGCGGAGCTCCATGCCACCAGTTCCAGCTCGAGGTCCTCAAGGACGGCGCCATTGTCGACCAATTCAATGTGTATGAAAAAGGGGCTTACATGTTCGGCCGGGTCGACCTCTGCGATTTTGTTCTGGAGCACCCTACTATTTCTCGGTTTCATGCAGTGCTCCAGTTCAAGAGAAGTGGAGATGCGTACATCTATGACCTTGGCAGTACTCATGGTACTTTTGTTAACAAGAACCAGGTCAGTAAAAAGGTGTATGTGGACTTGCATGTTGGTGATGTCATTCGTTTTGGGCATTCATCTCGTCTATACATTTTCCAAGGACCGTCTGAGTTGATGCCACCAGAAAAAGACTTGAAAGTTTTAAGAGAGTACAAGATGCGTGAAGATATTTTAGATCAGCAGGCATCACTTCAACGAGCAAGACTTGAAGCTTCTCTTGCTGATGGCATCTCTTGGGGTATGGGAGAGGATGCCATTATAGAAGAAGCCGAGGATGATGGTGAGGAAGTAACTTGGCAAACATACAAAGGACAGCTTACGGAAAAGCAGATAAAAACCCGTGATAAAATAACTAAAAGAATGGAAAAGATTGCTCATATGAAGAAAGAGATAGATGCTATTCGTGCTAAAGACATCTCTCAAGGTGGATTGACTCAAGGGCAACAAACTCAGATCGCGCGAAATGAACAAAGAACAGAACAGATTCTGGAAGAGCTTGAAAATTTGGAAGAGACACTGAATGAGAGTATTCGAGAAAGTTTAGGTGCACGTGTTGGGAAGCTATCTCATGGTAAGAAGAAAGGAGCAATTGAAGAAGAAGAAGAACTTTTGAGTGATGATGATGAGTTTTATGATCGGACAAAGAAGCCTTCTAGCAAAAAGGCTAGTGAAAACCCATCAATTGAAACCGCTGATACTCTTCTTGATAAGAGAGATGCAATCACAAAAGAAATGGAGAACACGAAAGAGCTGCTTTCAATTGAGAAAAACAGACTGGCATCAGAAACTACAGAAGACACTGATGCTGGGGACGCACTGGATGCATACATGTCTGGGCTTTCATCTAAGCTAGTGCTTGATAAAACTGAGCAATTGCAAAAGGAACTCTCAGATCTCCAGTCCGAATATGACAGGGTAGTCTTCCTTTTGAAGATTGCTGATCCAACAGGTGAAGCAGCCAAGAAAAGGGATTCAAAGGTGCTTCCTGAAAATACTGAAACTTCTGCTGCTGCCATCAAGAAGCAACATCCACTTAAACCAAAGGAAACCTGTCCGCCTGAAAATCCAGAAAGTGGCTCTATAAAGAAAGAGGAAAGTACAGATGTGACTGTTGCATCTAGCAAGAAGCTGGAGTCAGGTGAGGTTCTCACTGATGCAACTGAAGGAAAAAGTGTTGTATATACTGTTGCAAAGCCCCAGTGGCTTGGAGCTATAGTGGACACGAAGATGGTGGAGGGTCATCAAGAGGCAGCATCTACGAATGAGCACGAGTCTGAAGTATTTGTGGACTATAAAGACAGAAAGAAGATTTTGGAGAATGAAGTTAACATGGAATCTGGGATTGAAAATGCTGCGCCTGGTTTGATTATAAGGAAACGAAAACAAGTTCATGAATCTGAAGCTAGTGATGATTCTCATCAACTTTCGACATCTTCCTCCACAGGAGCTGGACTCGTGGCAGAGGATGCTGTAGCACTGTTGTTGAAGCATAACAGAGGCTATTATGCATCAGATGATGATAAATCCTCTGAAAGCCAAGATACGTCACAAGGGAAACAACAGAGCAAGGGTAAAAAGCCCAAAAAAAGAGTACTTGGTCCTGAGAGACCTTCATTTCTTGATAGTGATTCTACTGAAACATGGGTGCCTCCTGAAGGGCAATCTGGTGATGGGCGAACATCCTTGAACGATCGATATGGCTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

737

Amino Acids

81.56

Weight (kDa)

5.01

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Yop-YscD_cpl PF16697 122 - 194 5.6e-09 Inner membrane component of T3SS, cytoplasmic domain
FHA PF00498 126 - 193 8.3e-21 FHA domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1877
AasI GACNNNNNNGTC 1 cut(s) 1424
AccB1I GGYRCC 2 cut(s) 335, 2152
AccB7I CCANNNNNTGG 1 cut(s) 1580
AccBSI CCGCTC 1 cut(s) 291
AccI GTMKAC 2 cut(s) 345, 393
AccII CGCG 3 cut(s) 224, 965, 967
AclWI GGATC 1 cut(s) 1442
AcoI YGGCCR 1 cut(s) 385
AcsI RAATTY 1 cut(s) 1009
AcuI CTGAAG 4 cut(s) 1689, 1803, 1926, 2181
AcvI CACGTG 1 cut(s) 1058
AcyI GRCGYC 1 cut(s) 336
AfaI GTAC 6 cut(s) 476, 496, 505, 659, 1606, 2100
AfiI CCNNNNNNNGG 6 cut(s) 128, 129, 319, 1473, 1580, 2162
AflIII ACRYGT 3 cut(s) 378, 1057, 1335
AjnI CCWGG 2 cut(s) 522, 1864
Alw21I GWGCWC 5 cut(s) 298, 420, 450, 1057, 1776
Alw26I GTCTC 4 cut(s) 83, 227, 1013, 2107
Alw44I GTGCAC 1 cut(s) 1053
AlwI GGATC 1 cut(s) 1442
AlwNI CAGNNNCTG 2 cut(s) 994, 1955
Ama87I CYCGRG 1 cut(s) 317
AoxI GGCC 2 cut(s) 131, 385
ApaLI GTGCAC 1 cut(s) 1053
ApeKI GCWGC 6 cut(s) 1258, 1463, 1514, 1517, 1757, 1859
ApoI RAATTY 1 cut(s) 1009
Asp700I GAANNNNTTC 1 cut(s) 990
AspLEI GCGC 4 cut(s) 226, 338, 967, 1864
AspS9I GGNCC 5 cut(s) 17, 131, 322, 608, 2105
AsuC2I CCSGG 1 cut(s) 389
AsuHPI GGTGA 5 cut(s) 572, 800, 1469, 1658, 2186
AvaI CYCGRG 1 cut(s) 317
AvaII GGWCC 4 cut(s) 17, 322, 608, 2105
BaeGI GKGCMC 1 cut(s) 1057
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BanI GGYRCC 2 cut(s) 335, 2152
BanII GRGCYC 1 cut(s) 298
BauI CACGAG 2 cut(s) 1775, 1961
BbrPI CACGTG 1 cut(s) 1058
BbsI GAAGAC 2 cut(s) 1308, 1420
Bbv12I GWGCWC 5 cut(s) 298, 420, 450, 1057, 1776
BbvI GCAGC 6 cut(s) 1245, 1475, 1501, 1504, 1769, 1846
BccI CCATC 8 cut(s) 79, 121, 725, 779, 1192, 1529, 1729, 2171
BceAI ACGGC 2 cut(s) 248, 349
BcgI CGANNNNNNTGC 4 cut(s) 569, 603, 1240, 1274
BciT130I CCWGG 2 cut(s) 524, 1866
BcnI CCSGG 1 cut(s) 389
BcoDI GTCTC 4 cut(s) 83, 227, 1013, 2107
BfaI CTAG 7 cut(s) 71, 153, 1161, 1173, 1358, 1626, 1911
BfmI CTRYAG 3 cut(s) 1296, 1719, 1977
BfoI RGCGCY 1 cut(s) 339
BglI GCCNNNNNGGC 1 cut(s) 1708
BglII AGATCT 1 cut(s) 1399
BisI GCNGC 8 cut(s) 26, 227, 1259, 1464, 1515, 1518, 1758, 1860
BlsI GCNGC 8 cut(s) 27, 228, 1260, 1465, 1516, 1519, 1759, 1861
BmcAI AGTACT 2 cut(s) 496, 2100
Bme1390I CCNGG 3 cut(s) 389, 524, 1866
Bme18I GGWCC 4 cut(s) 17, 322, 608, 2105
BmeT110I CYCGRG 1 cut(s) 317
BmgT120I GGNCC 5 cut(s) 17, 131, 322, 608, 2105
BmiI GGNNCC 4 cut(s) 18, 132, 337, 2154
BmrFI CCNGG 3 cut(s) 389, 524, 1866
BmrI ACTGGG 1 cut(s) 1699
BmuI ACTGGG 1 cut(s) 1699
BpiI GAAGAC 2 cut(s) 1308, 1420
BplI GAGNNNNNCTC 6 cut(s) 80, 112, 280, 312, 1944, 1976
BpmI CTGGAG 4 cut(s) 434, 434, 1388, 1658
BpuEI CTTGAG 3 cut(s) 311, 912, 927
BpuMI CCSGG 1 cut(s) 389
Bsa29I ATCGAT 1 cut(s) 2200
BsaAI YACGTR 1 cut(s) 1058
BsaBI GATNNNNATC 1 cut(s) 1923
BsaHI GRCGYC 1 cut(s) 336
BsaI GGTCTC 2 cut(s) 83, 2107
BsaJI CCNNGG 5 cut(s) 12, 253, 487, 604, 777
BsaXI ACNNNNNCTCC 2 cut(s) 1707, 1737
Bsc4I CCNNNNNNNGG 6 cut(s) 128, 129, 319, 1473, 1580, 2162
Bse1I ACTGG 6 cut(s) 307, 451, 1287, 1329, 1405, 1705
Bse8I GATNNNNATC 1 cut(s) 1923
BseBI CCWGG 2 cut(s) 524, 1866
BseCI ATCGAT 1 cut(s) 2200
BseDI CCNNGG 5 cut(s) 12, 253, 487, 604, 777
BseGI GGATG 8 cut(s) 200, 236, 763, 787, 1333, 1534, 1978, 2186
BseJI GATNNNNATC 1 cut(s) 1923
BseLI CCNNNNNNNGG 6 cut(s) 128, 129, 319, 1473, 1580, 2162
BseMII CTCAG 5 cut(s) 606, 971, 1365, 1410, 2100
BseNI ACTGG 6 cut(s) 307, 451, 1287, 1329, 1405, 1705
BseRI GAGGAG 2 cut(s) 153, 156
BseSI GKGCMC 1 cut(s) 1057
BseX3I CGGCCG 1 cut(s) 385
BseXI GCAGC 6 cut(s) 1245, 1475, 1501, 1504, 1769, 1846
BseYI CCCAGC 1 cut(s) 1313
Bsh1236I CGCG 3 cut(s) 224, 965, 967
Bsh1285I CGRYCG 3 cut(s) 9, 388, 2200
BshFI GGCC 2 cut(s) 133, 387
BshNI GGYRCC 2 cut(s) 335, 2152
BshVI ATCGAT 1 cut(s) 2200
BsiEI CGRYCG 3 cut(s) 9, 388, 2200
BsiHKAI GWGCWC 5 cut(s) 298, 420, 450, 1057, 1776
BsiHKCI CYCGRG 1 cut(s) 317
BsiSI CCGG 2 cut(s) 230, 388
BslFI GGGAC 2 cut(s) 251, 1331
BslI CCNNNNNNNGG 6 cut(s) 128, 129, 319, 1473, 1580, 2162
BsmAI GTCTC 4 cut(s) 83, 227, 1013, 2107
BsmFI GGGAC 2 cut(s) 251, 1331
BsmI GAATGC 1 cut(s) 580
BsnI GGCC 2 cut(s) 133, 387
Bso31I GGTCTC 2 cut(s) 83, 2107
BsoBI CYCGRG 1 cut(s) 317
Bsp1286I GDGCHC 5 cut(s) 298, 420, 450, 1057, 1776
Bsp143I GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
Bsp19I CCATGG 1 cut(s) 12
BspANI GGCC 2 cut(s) 133, 387
BspCNI CTCAG 5 cut(s) 607, 970, 1366, 1409, 2101
BspDI ATCGAT 1 cut(s) 2200
BspFNI CGCG 3 cut(s) 224, 965, 967
BspHI TCATGA 1 cut(s) 1897
BspLI GGNNCC 4 cut(s) 18, 132, 337, 2154
BspPI GGATC 1 cut(s) 1442
BspQI GCTCTTC 1 cut(s) 993
BspT107I GGYRCC 2 cut(s) 335, 2152
BspTNI GGTCTC 2 cut(s) 83, 2107
BsrBI CCGCTC 1 cut(s) 291
BsrI ACTGG 6 cut(s) 307, 451, 1287, 1329, 1405, 1705
BssECI CCNNGG 5 cut(s) 12, 253, 487, 604, 777
BssMI GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
BssNI GRCGYC 1 cut(s) 336
BssSI CACGAG 2 cut(s) 1775, 1961
BssT1I CCWWGG 4 cut(s) 12, 253, 487, 604
Bst2BI CACGAG 2 cut(s) 1775, 1961
Bst2UI CCWGG 2 cut(s) 524, 1866
Bst4CI ACNGT 4 cut(s) 612, 1618, 1693, 1986
Bst6I CTCTTC 3 cut(s) 993, 1011, 1212
BstACI GRCGYC 1 cut(s) 336
BstAPI GCANNNNNTGC 2 cut(s) 1328, 1973
BstBAI YACGTR 1 cut(s) 1058
BstC8I GCNNGC 1 cut(s) 690
BstDEI CTNAG 6 cut(s) 615, 957, 1353, 1374, 1396, 2109
BstDSI CCRYGG 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 2160
BstF5I GGATG 8 cut(s) 200, 236, 763, 787, 1333, 1534, 1978, 2186
BstFNI CGCG 3 cut(s) 224, 965, 967
BstH2I RGCGCY 1 cut(s) 339
BstHHI GCGC 4 cut(s) 226, 338, 967, 1864
BstKTI GATC 6 cut(s) 685, 963, 1144, 1402, 1450, 2200
BstMAI GTCTC 4 cut(s) 83, 227, 1013, 2107
BstMBI GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
BstMCI CGRYCG 3 cut(s) 9, 388, 2200
BstMWI GCNNNNNNNGC 8 cut(s) 31, 53, 725, 911, 1319, 1328, 1708, 1973
BstNI CCWGG 2 cut(s) 524, 1866
BstNSI RCATGY 3 cut(s) 382, 557, 1339
BstSCI CCNGG 3 cut(s) 387, 522, 1864
BstSFI CTRYAG 3 cut(s) 1296, 1719, 1977
BstSLI GKGCMC 1 cut(s) 1057
BstUI CGCG 3 cut(s) 224, 965, 967
BstV1I GCAGC 6 cut(s) 1245, 1475, 1501, 1504, 1769, 1846
BstV2I GAAGAC 2 cut(s) 1308, 1420
BstX2I RGATCY 1 cut(s) 1399
BstXI CCANNNNNNTGG 1 cut(s) 1712
BstYI RGATCY 1 cut(s) 1399
BstZI CGGCCG 1 cut(s) 385
Bsu15I ATCGAT 1 cut(s) 2200
BsuRI GGCC 2 cut(s) 133, 387
BsuTUI ATCGAT 1 cut(s) 2200
BtgI CCRYGG 1 cut(s) 12
BtsCI GGATG 8 cut(s) 200, 236, 763, 787, 1333, 1534, 1978, 2186
BtsI GCAGTG 1 cut(s) 450
BtsIMutI CAGTG 7 cut(s) 450, 1022, 1305, 1322, 1656, 1712, 1982
Cac8I GCNNGC 1 cut(s) 690
CaiI CAGNNNCTG 2 cut(s) 994, 1955
CciI TCATGA 1 cut(s) 1897
CfoI GCGC 4 cut(s) 226, 338, 967, 1864
Cfr13I GGNCC 5 cut(s) 17, 131, 322, 608, 2105
ClaI ATCGAT 1 cut(s) 2200
CseI GACGC 1 cut(s) 1328
CsiI ACCWGGT 1 cut(s) 522
Csp6I GTAC 6 cut(s) 475, 495, 504, 658, 1605, 2099
CspCI CAANNNNNGTGG 2 cut(s) 445, 480
CviQI GTAC 6 cut(s) 475, 495, 504, 658, 1605, 2099
DdeI CTNAG 6 cut(s) 615, 957, 1353, 1374, 1396, 2109
DinI GGCGCC 1 cut(s) 337
DpnI GATC 6 cut(s) 684, 962, 1143, 1401, 1449, 2199
DpnII GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
DrdI GACNNNNNNGTC 1 cut(s) 1424
DseDI GACNNNNNNGTC 1 cut(s) 1424
EaeI YGGCCR 1 cut(s) 385
EagI CGGCCG 1 cut(s) 385
Eam1104I CTCTTC 3 cut(s) 993, 1011, 1212
EarI CTCTTC 3 cut(s) 993, 1011, 1212
EciI GGCGGA 3 cut(s) 12, 21, 1551
Ecl136II GAGCTC 1 cut(s) 296
EclXI CGGCCG 1 cut(s) 385
Eco130I CCWWGG 4 cut(s) 12, 253, 487, 604
Eco24I GRGCYC 1 cut(s) 298
Eco31I GGTCTC 2 cut(s) 83, 2107
Eco47I GGWCC 4 cut(s) 17, 322, 608, 2105
Eco52I CGGCCG 1 cut(s) 385
Eco53kI GAGCTC 1 cut(s) 296
Eco57I CTGAAG 4 cut(s) 1689, 1803, 1926, 2181
Eco72I CACGTG 1 cut(s) 1058
Eco88I CYCGRG 1 cut(s) 317
EcoICRI GAGCTC 1 cut(s) 296
EcoNI CCTNNNNNAGG 1 cut(s) 2160
EcoO109I RGGNCCY 3 cut(s) 17, 131, 322
EcoRII CCWGG 2 cut(s) 522, 1864
EcoT14I CCWWGG 4 cut(s) 12, 253, 487, 604
EcoT22I ATGCAT 2 cut(s) 1333, 2017
EcoT38I GRGCYC 1 cut(s) 298
EgeI GGCGCC 1 cut(s) 337
EheI GGCGCC 1 cut(s) 337
ErhI CCWWGG 4 cut(s) 12, 253, 487, 604
FalI AAGNNNNNCTT 8 cut(s) 708, 740, 807, 839, 1098, 1130, 1245, 1277
FaqI GGGAC 2 cut(s) 251, 1331
FauI CCCGC 2 cut(s) 52, 61
FauNDI CATATG 1 cut(s) 885
FblI GTMKAC 2 cut(s) 345, 393
Fnu4HI GCNGC 8 cut(s) 26, 227, 1259, 1464, 1515, 1518, 1758, 1860
FokI GGATG 8 cut(s) 187, 223, 770, 794, 1340, 1521, 1985, 2173
FriOI GRGCYC 1 cut(s) 298
Fsp4HI GCNGC 8 cut(s) 26, 227, 1259, 1464, 1515, 1518, 1758, 1860
FspBI CTAG 7 cut(s) 71, 153, 1161, 1173, 1358, 1626, 1911
GlaI GCGC 4 cut(s) 225, 337, 966, 1863
GluI GCNGC 8 cut(s) 26, 227, 1259, 1464, 1515, 1518, 1758, 1860
GsaI CCCAGC 1 cut(s) 1317
GsuI CTGGAG 4 cut(s) 434, 434, 1388, 1658
HaeII RGCGCY 1 cut(s) 339
HaeIII GGCC 2 cut(s) 133, 387
HapII CCGG 2 cut(s) 230, 388
HgaI GACGC 1 cut(s) 1328
HhaI GCGC 4 cut(s) 226, 338, 967, 1864
Hin1I GRCGYC 1 cut(s) 336
Hin6I GCGC 4 cut(s) 224, 336, 965, 1862
HinP1I GCGC 4 cut(s) 224, 336, 965, 1862
HincII GTYRAC 4 cut(s) 346, 394, 514, 1834
HindII GTYRAC 4 cut(s) 346, 394, 514, 1834
HindIII AAGCTT 1 cut(s) 717
HpaI GTTAAC 2 cut(s) 514, 1834
HpaII CCGG 2 cut(s) 230, 388
HphI GGTGA 5 cut(s) 572, 800, 1469, 1658, 2186
Hpy166II GTNNAC 9 cut(s) 158, 346, 394, 514, 547, 1055, 1726, 1795, 1834
Hpy8I GTNNAC 9 cut(s) 158, 346, 394, 514, 547, 1055, 1726, 1795, 1834
HpyAV CCTTC 4 cut(s) 1167, 1664, 2127, 2156
HpyCH4III ACNGT 4 cut(s) 612, 1618, 1693, 1986
HpyCH4IV ACGT 2 cut(s) 1057, 2051
HpyF10VI GCNNNNNNNGC 8 cut(s) 31, 53, 725, 911, 1319, 1328, 1708, 1973
HpyF3I CTNAG 6 cut(s) 615, 957, 1353, 1374, 1396, 2109
HpySE526I ACGT 2 cut(s) 1057, 2051
Hsp92I GRCGYC 1 cut(s) 336
HspAI GCGC 4 cut(s) 224, 336, 965, 1862
KasI GGCGCC 1 cut(s) 335
KspAI GTTAAC 2 cut(s) 514, 1834
Kzo9I GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
LguI GCTCTTC 1 cut(s) 993
LmnI GCTCC 8 cut(s) 288, 293, 301, 415, 453, 1091, 1715, 1952
Lsp1109I GCAGC 6 cut(s) 1245, 1475, 1501, 1504, 1769, 1846
MabI ACCWGGT 1 cut(s) 522
MaeI CTAG 7 cut(s) 71, 153, 1161, 1173, 1358, 1626, 1911
MaeII ACGT 2 cut(s) 1057, 2051
MaeIII GTNAC 3 cut(s) 796, 1612, 2052
MalI GATC 6 cut(s) 684, 962, 1143, 1401, 1449, 2199
MbiI CCGCTC 1 cut(s) 291
MboI GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
MfeI CAATTG 4 cut(s) 1095, 1188, 1266, 1379
MflI RGATCY 1 cut(s) 1399
MhlI GDGCHC 5 cut(s) 298, 420, 450, 1057, 1776
MluCI AATT 6 cut(s) 350, 1009, 1095, 1188, 1266, 1379
Mly113I GGCGCC 1 cut(s) 336
MlyI GAGTC 6 cut(s) 53, 206, 934, 1649, 1787, 1953
MmeI TCCRAC 1 cut(s) 1475
Mph1103I ATGCAT 2 cut(s) 1333, 2017
MroXI GAANNNNTTC 1 cut(s) 990
MseI TTAA 4 cut(s) 513, 650, 1542, 1833
MslI CAYNNNNRTG 2 cut(s) 558, 1733
MspA1I CMGCKG 1 cut(s) 1199
MspI CCGG 2 cut(s) 230, 388
MspR9I CCNGG 3 cut(s) 389, 524, 1866
MunI CAATTG 4 cut(s) 1095, 1188, 1266, 1379
Mva1269I GAATGC 1 cut(s) 580
MvaI CCWGG 2 cut(s) 524, 1866
MvnI CGCG 3 cut(s) 224, 965, 967
MwoI GCNNNNNNNGC 8 cut(s) 31, 53, 725, 911, 1319, 1328, 1708, 1973
NarI GGCGCC 1 cut(s) 336
NciI CCSGG 1 cut(s) 389
NcoI CCATGG 1 cut(s) 12
NdeI CATATG 1 cut(s) 885
NdeII GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
NlaIV GGNNCC 4 cut(s) 18, 132, 337, 2154
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 2 cut(s) 1612, 2052
NsiI ATGCAT 2 cut(s) 1333, 2017
NspI RCATGY 3 cut(s) 382, 557, 1339
PaeR7I CTCGAG 1 cut(s) 317
PagI TCATGA 1 cut(s) 1897
PciI ACATGT 2 cut(s) 378, 1335
PciSI GCTCTTC 1 cut(s) 993
PcsI WCGNNNNNNNCGW 2 cut(s) 390, 399
PctI GAATGC 1 cut(s) 580
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 7 cut(s) 277, 991, 1478, 1841, 1901, 1919, 2135
PflMI CCANNNNNTGG 1 cut(s) 1580
PkrI GCNGC 8 cut(s) 27, 228, 1260, 1465, 1516, 1519, 1759, 1861
Ple19I CGATCG 1 cut(s) 2200
PleI GAGTC 6 cut(s) 53, 206, 934, 1648, 1786, 1953
PluTI GGCGCC 1 cut(s) 339
PmaCI CACGTG 1 cut(s) 1058
PmlI CACGTG 1 cut(s) 1058
PpsI GAGTC 6 cut(s) 53, 206, 934, 1648, 1786, 1953
Ppu21I YACGTR 1 cut(s) 1058
PpuMI RGGWCCY 2 cut(s) 17, 322
PscI ACATGT 2 cut(s) 378, 1335
PsiI TTATAA 1 cut(s) 1877
Psp124BI GAGCTC 1 cut(s) 298
Psp5II RGGWCCY 2 cut(s) 17, 322
Psp6I CCWGG 2 cut(s) 522, 1864
PspCI CACGTG 1 cut(s) 1058
PspFI CCCAGC 1 cut(s) 1313
PspGI CCWGG 2 cut(s) 522, 1864
PspN4I GGNNCC 4 cut(s) 18, 132, 337, 2154
PspPI GGNCC 5 cut(s) 17, 131, 322, 608, 2105
PspPPI RGGWCCY 2 cut(s) 17, 322
PspXI VCTCGAGB 1 cut(s) 317
PstNI CAGNNNCTG 2 cut(s) 994, 1955
PsuI RGATCY 1 cut(s) 1399
PvuI CGATCG 1 cut(s) 2200
RsaI GTAC 6 cut(s) 476, 496, 505, 659, 1606, 2100
RsaNI GTAC 6 cut(s) 475, 495, 504, 658, 1605, 2099
RseI CAYNNNNRTG 2 cut(s) 558, 1733
SacI GAGCTC 1 cut(s) 298
SalI GTCGAC 2 cut(s) 344, 392
SapI GCTCTTC 1 cut(s) 993
SaqAI TTAA 4 cut(s) 513, 650, 1542, 1833
SatI GCNGC 8 cut(s) 26, 227, 1259, 1464, 1515, 1518, 1758, 1860
Sau3AI GATC 6 cut(s) 682, 960, 1141, 1399, 1447, 2197
Sau96I GGNCC 5 cut(s) 17, 131, 322, 608, 2105
ScaI AGTACT 2 cut(s) 496, 2100
SchI GAGTC 6 cut(s) 53, 206, 934, 1649, 1787, 1953
ScrFI CCNGG 3 cut(s) 389, 524, 1866
SduI GDGCHC 5 cut(s) 298, 420, 450, 1057, 1776
SexAI ACCWGGT 1 cut(s) 522
SfcI CTRYAG 3 cut(s) 1296, 1719, 1977
SfoI GGCGCC 1 cut(s) 337
Sfr274I CTCGAG 1 cut(s) 317
SinI GGWCC 4 cut(s) 17, 322, 608, 2105
SlaI CTCGAG 1 cut(s) 317
SmiMI CAYNNNNRTG 2 cut(s) 558, 1733
SmlI CTYRAG 4 cut(s) 317, 326, 927, 942
SmoI CTYRAG 4 cut(s) 317, 326, 927, 942
Sse9I AATT 6 cut(s) 350, 1009, 1095, 1188, 1266, 1379
SspDI GGCGCC 1 cut(s) 335
SspMI CTAG 7 cut(s) 71, 153, 1161, 1173, 1358, 1626, 1911
SstI GAGCTC 1 cut(s) 298
StyD4I CCNGG 3 cut(s) 387, 522, 1864
StyI CCWWGG 4 cut(s) 12, 253, 487, 604
TaaI ACNGT 4 cut(s) 612, 1618, 1693, 1986
TaiI ACGT 2 cut(s) 1060, 2054
TaqI TCGA 7 cut(s) 275, 318, 345, 393, 1039, 1934, 2200
TasI AATT 6 cut(s) 350, 1009, 1095, 1188, 1266, 1379
TatI WGTACW 4 cut(s) 494, 657, 1604, 2098
TauI GCSGC 2 cut(s) 28, 229
TfiI GAWTC 7 cut(s) 277, 991, 1478, 1841, 1901, 1919, 2135
Tru1I TTAA 4 cut(s) 513, 650, 1542, 1833
Tru9I TTAA 4 cut(s) 513, 650, 1542, 1833
TscAI CASTG 7 cut(s) 450, 1029, 1312, 1329, 1663, 1712, 1989
TseFI GTSAC 2 cut(s) 1612, 2052
TseI GCWGC 6 cut(s) 1258, 1463, 1514, 1517, 1757, 1859
Tsp45I GTSAC 2 cut(s) 1612, 2052
TspGWI ACGGA 1 cut(s) 842
TspRI CASTG 7 cut(s) 450, 1029, 1312, 1329, 1663, 1712, 1989
Van91I CCANNNNNTGG 1 cut(s) 1580
VneI GTGCAC 1 cut(s) 1053
VpaK11BI GGWCC 4 cut(s) 17, 322, 608, 2105
XagI CCTNNNNNAGG 1 cut(s) 2160
XapI RAATTY 1 cut(s) 1009
XceI RCATGY 3 cut(s) 382, 557, 1339
XhoI CTCGAG 1 cut(s) 317
XmiI GTMKAC 2 cut(s) 345, 393
XmnI GAANNNNTTC 1 cut(s) 990
XspI CTAG 7 cut(s) 71, 153, 1161, 1173, 1358, 1626, 1911
ZrmI AGTACT 2 cut(s) 496, 2100
Zsp2I ATGCAT 2 cut(s) 1333, 2017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.