pycom17g02230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0001371
Physical Location & Seq
Forward (+)
1422332 .. 1437148
14817 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g02230.8

Sequence Viewer

Length: 7422 bp
ATGGCAGCGGAAGCTGAGGTTCCGGAATCTGTATCCGCCACGGCGGAAGGGGAAGACGGATATCTGACAACGGGGAAGTTTGACATAACTGTCAACCAATTTTCCCCAGTACAATTGAAAAAATCCGAAGGCGAGGAGTGTCAACATCCTGAACAGGTGAAATGCTTTGTAGAGGGTGAAATTGCACACGTTGAAAGCAAGAACGGAATCTCAGAGTGTAGAAATCCTTTAGAAACACTCTCAGATTCAAAGGTTGACAAACAAGAGGAAGATGCACGGAGAAAAGGTGAGGCACTGGATATAGGAGAGGCTTCAGTGGTGGAGGAGGAAGATAGTGATGGATTTTCCGCTGTAGAGCACAGCAAAGCGAATTCAATAGATCACAAAGGAGAAAGCGAGACTAACTTATATGAGAACATTCAGCCTGTCACCTATGAGCTTGCTAGACCGAAGTTAAATTCTGAGGAAGACAAAGCAGCAACTTCGAGAGAAAGTTCAGAAACAATCACCACTGCTGAAGATGAGAGACGAGTTGTAGAACAAAGCTGCGAGAAAGAGGGAAGGGTAGAGGAAGCAAATACTAGTTATGACACCATTGAAGATGAAACTCCTGACGAACAGAATCTCCCAGTGGCAAGTAGCTTGATGCAAATTGTTACGGAGTCAGAGATTGCTGAAGTAGGCGAGAGCACCATATCGGAGCCAAATGAAAATGTTGAAAGATCTGAAGGAGTTCTTAATGTTTCAACGGTGAAAGGTGACGAAGGTGATCAGGGAGATGGTACCAACCCAACTAAGGATGACAAAGATGTAGATACATTTACCACCAAAGTGGCTGAAGATGATGAGTATACTAGTGCAGTCACGATTAAAGAAACGAGCACAAAAGTAACACCAAATGATGATGAAAGTTCACCACATACCTTGCAGAAATGTGATTCAGGAGAGGAGCTTCAACATTCGCGGGATGTGTTGCCTGAGGAAACTAAGACAGAAACATCCCGGGAAAATGCAGAAGAGAGTACCTGCACAAAAGAGGAAACAACAATTCAGCACACGCAAGAATCCTTGGACGAAGAAAAGAAACAAGCTACTGAAAACACAAGTAATTTAGAAGATGACAATGCAAGGGAGGAGGTGCTTGAGACTTCCGGTACAAAACAAGAAACCGGAGAACACTTGGTTGGGGAAATTCATACGGAATCCCCTCAAGATTTCGTTGATAAAGAAACAAATGAAGAAACCTCCACAGAGTATCAGGCGTGTGTTGAGGAAGCCGATTTGAGTACAAATACAGAGGAAAGGAACTCAGAGGAACAGGCCATTCCGGAACTCTCTTCTCCGTTGGTAGTAGAAGAAACAGCTAAGGAAATCCCAAGGGAAGAAGAAACCGGTGTCTTGATTCTAAAAGAAGAGAAAAAGGAAGAAGATGCAGAATGTAACGAGCCCGCTGAGGTAGCGAGTATGACACAAGAAACACAATCTGAAACCACAGACGACATAACCGAGCCAAATAAGGACCTCAGTGCTCCAATTGCAGAAGAAAGCAATAAAACATTTGTTGAAAATATTTCCACTCTTGCTGAGGTATCTGAGACGGAGACAGAGAAAACTATTCAGGAGAAAGATGGCCTGACGGTTAGCTATTCTGACTCTGCGTCGGGTGAAGTGAGTCATGTAACAGGTTTGAAGGAAGCTGAAACAGAAAAGGAGGAGCAGAAAGAAAACATTGATACTGCTCCGGAAGAGAAGGGTCAGGCAACCATCTTAAAAGATGAAATGGCCTCGGAAGTTGAAGATATTGGTGCACGAGCAGAGGAAAATTGTGAATCAAAAGAGACGTTGGAGGAGCATATTCCAACAGCAGCAGATAAGGGTGAAGCATTCTCGAAGGAAGCTGAGGCAGAAATTGAGGAGCAGATAAACAATAGTGATGTTGCTGCTGAAGAGAAGGTTCTGAGAACCATCTTAACAGATGAAAAATCCTTCGACGTGGAAGATACCAACGTGCAAGCAGAGAAAAATGTCGAATCAAATGAGAACTTGGAGGAGAATATTCCAACAGCAGCAGATGAAGGCAAAACATTCCCGAAGGAAGCTGAAGCAGAAAGCAAGGATGAGATTAAAAATGATGATATTCCTCATGCAGATATTGATGGACACGGAGATGAAATTGATAGTTCTGAAGAGAGCTTGGATAAGCAGATTCCAGCAGGGGAATGTGAAACAAGCTTGAAAGCATCCGAAGCAGAATATAAGGAGCTGGTCATACATGGTGATATTGCTCCTGAAGAGAAGGGTCTAGCAACCATCGTAACAGATGAAACACCCTTAGACGTCGAGGATATTGAGGTAAAAACTGAGGACTCTGCCGAATCAAAAGAGACTGTGGAGGAGCAGATTCCAACAACAGCAGATGAAGGCGAAAAAAGTACTTATATTTCTCATGAAGATATTGAAGTACAAGCAGATGAAAATTTTATTACACTAGAGAACTTGGAGAACCTGATTCCAACAACATCAGGTGAACGAGAAACAGGCTTGGGACAAGCTGAAACAGAAATTAAGGAGCAGGTCAAAAGTATTGATATTGTTCCTGAAGAGATAGCAACCATCTCAACAACTGAGACACCCCTGGACATTGAAGACATCGGTGTACAAGCAGAAGAAAGTCTCAAATCAAAAGAGAATGTGGAGGAGCAGATTCCAACAGCAGCAGATGAAGATGAAAATTTCTTGAAGGAAGCCGAAGCAGAAAACAAGGAGCAGATTGACGAGGAAACTTTGAATTCACAAGAATCCTCAGACAAAGAAAAAGAAGAAGTGACTGAAATTACAAACACAAAAGATGACACTGAAAGAAAGGAGGTGCTTGAGACTACCAGCATGGAGCAAGAAACTGGAGAACATGTGGTACACAAAATTCATACTGTCCCAGAACCCCCTCGAGATTCCATTGATAAAGAAACAAATGAAGACAACTCCGCGGAATATCAGGAATCTACTGAGGCAGCTGATTTGAGTGCAAGTGCAGAGGAAAAGAACTTAGAGGAACATGCCATTCAGGAACTCTCTTCTCCGCTGGAAGTAGAAGAAGCAACTAACCAAATCCTGAAGGAAGAAGAAACCTACACCACGCTTCTAGAAGAAGAGGAAACGGAAGGGCATGCAACATGTAATGAGCCCAATGAGGAATACGAGGCAGAGATTGAGAAAAATATTCAGGAGAAAGATGGCAACACAGGTAGCAATACGGAATCTCTGTCCGGAGAACTGAGTCATGTAACAGCCTTGAAGGAATCTGAAACAGAGATCGAAGGGCAGGACAAGAACATTGATACTGCTCCGGAAGAGAAGGGCCAAGCAACCATCTCAGCAGATGAAACGACCTCGGAAGTTGAAGATATTGATGTACAATTAAAAGAGAACCTTGAGTCGGAAGAGAACTTGGAGGAACAATTTCCGATAGCAGCAGATGAAGGTGAAATAATCTCGAAAGAAGCCGAAGCAGAAAACCAGGAGCAGATAAAAAATGTTGATATTGCTCATGAAGATCTTGATGCACAAGCGGAGAAAACTGTCAACTCAGTAGAGAATATAGATAAGCAGATTCCGCCAATAGCACATGAAGGTGAAACAAGCTTGAAAGAAACCGCTGTCTTGATTCTAAAAGAAGAGAAAAAGGAAGAAGATACAGAATGTAACGAGCCTGCTGAGGTAGCGAGTATGACACATGAAACACAATCTAAAACCACAGAGGACAGAACAGAGCCAAATAAGGACCTCAATGCTCCAATTTCAGGAGAAAGCAACAAAACATTTGTCGAAAATATTTCCACTCTCGCTGAGGTATCTGAGACAGAGACTGAGAAAAATGTTGAGGAGAAAAATGGCCTGGCTGTCAGCAATTCTGACTTTGCGTCGGGTGAAGTGAGTCATGTAACAGGTTTGAAGGAAGCTGAAACAGAAAAGGAGGAGCAGAAAGAATACATTGATACTGCTCCAGAAGAGAAGGGTCAAGCAACCATCTTAAAAGATGAAATGGCCTCGGAAGTTGAAGATATTGGTGTACAAGCAGAGGAAAATTGTGAATCAAAAGAGAAGTTGGAGGAGCATATTCCAACAGCAGCAGATGAGGATGAAAAATTCTTGAAGGAATCCGAAGCAGAAAACAAGGAGCAGATTGACGAGGAAACTTTGAATTCACAAGAATCCTCAGACAAAAAAAAAGAAGTGACTGAAATTACAAACACAAAAGATGACACTGAAAGGGAGGAGGTGCTTGAGACTACCAGTATGGAGCAAGAAACTGGAGAACATGTGGTATACGAAATTCATACTGTCCTGAAACCACCTCGAGATTCCATTGATAAAGAAACAAATGAAGACAACTCCGCGGAATATCAGGCATGTTCTGAGGCAGCTGATTTGAGTACAAGTGCAGAGGAAAAGAACTTAGAGGAACATGACATTCAGGAACTCTCTTCTCCGCTGGTAGTAGAAGAAGCAACTAACCAAATCCTGAAGGAAGAAGAAACCTACACCACGCTTCTAGAAGAAGAGGAAAAGGAAGAGCATGCAACATGTAACGAGCCCAGTGAGGAATATGAGGCAGAGATTGAGAAAAATATTCAGGAGAAAGATGGCAACACAGTTAGCAATTCTGAATCTCTGTCCAGAGAACTGAGTCATGTAACAGCCTTGAAAGAATCTGAAACGGAGATCGAAAGGCAGGACAAAAACATTGATACTGCTCCTAAAGAGAGGGGTCAAGCAACCATCTCAGCAGATGAAATGGCCTCGGAAGTTGAAGATATTGATGTACAATTAAAGGAGAACCTTGAGTCGGAAGAGACCTTGGAGGAACAAGTTCCGATAGCAGCAGATGAAGGTGAAATAATCTCGAAAGAAGCTGAAGCAGAAAACCAGGAGCAGATAAAAAATGTTGATATTGCTCATGAAGATGTTGATGCACAAGAGGAGAAAGCTGTCAACTCAGATGAGAACATAGATAAGCAGATTCCGCCAATAGCACATGAAGTGACTGAAACTACAAACACACTAGAAGATGACACTGCAAGGGAGGAGGTGCTTGAGACTACCAGCACGGAGCAAGAAACTGGAGAACATGTGGTACACGAAATTCATACTGTCGTGGAACCCCCTCGAGATTCCATTGATAAAGAAACAAATGAAGAGAACTCCGCGGGATATCAGGCCTGTACTGATGCAGCTGATTTGAGTACAACTGCAGAGGAAAAGAACTTAGAGGAACATGACATTCAGGAACTCTCTTCTCCGCTGGTAGTAGAAGAAGAAACTAATGAGATCCCGAAGGAAGAAGAAACCAACGCTGTGCTTCTAGAAGAGGAAAATGAAGGTCATGCAACATGTAACGAGCCGACTGAGAAATATGGGGCAGAGATTGAGAAAAATATTCAGGAGAACGATGGCAACACAGTTAGCAATTCTGAATCTCTGTCTGGAGAACTGAGTCATGTAACAACCTTGCATGAATCTGAAACAGAGATCGAAGGGAAGGACAAAAACATTGATACTGCTGCTGAAGACAAGCGTCAAGCAACCATCTTAGCGGATGAAACGGCCTCAGAAGTTGAAGATATTGATGTACAATTAAAGGAGAATCTTGATTCAGAAGAGAACTTGGAGGAACAATTTCCTATAGCAGCAGATGAAGGTGAAATAATCTCGAAAGAAGCCGAAGCTGAAAACAAGGAGCAGATAAAAAATGTTGATATTGCTCATGAAGATGTTGATGCACAAGCAGGGCAAACTGTCAATTCAGAAGAGAACATAGATAAGCAGATTTCGCCAATAGCACATGAAGGTGAAACAAGCTTGAAAGAAGCCGAAGCAGAAGATAAGGAACAGGTCACATGTGCTGATATTGCTCCTGAAGAGAAGGGTCTAGCGACCATCTTAACAGATGAGACACCCTTGGAAGCAGAAGATATTGGTGTAAAAGCAGAGGAAAGTTTCGAATCAAAAGAGAAGTCCGAGGAGAAAAATCCAATTGCAGCTGATGAAGATGAAACATTCTTGAAGGAAGCCGAAGCAGAAAACAAGGAGCAGATAAAATATAGTGATATTGCTCCTGAAGACAAGGGTCTAGCAACCATCTTAACAGATCAAAAGGCTTCGAACATTGAAGATATTGGTTTACAAGCGGAGGAAACTTTCAACTCGAGCAAGAACTTGGAGAGTAAGATTCCATCAACAGCAGATGAACATGAAACAAGCTTGAAAGAAGCCGAAGCAATAAATACGGAGCAGTTCAAATACACTGATATTGTTCCAGAAGAGAAGGGTCTTGCAAACATCGTAATGGATGAAACATCTTTGGACGTAGAAGATATTGATGTAAAATCGCATGAAGTTTCCGAATCAAAAGAGAACGTGGAAGAGCAGATTCCAACCGCATCACATGAGCGTGGAACATTCTTGAATGAAGCTGAAGCAGAAAGTAAGGAGCATAAATACAATACCGATATTTCTCCAGAAGAGAAGAGTCTAGCAACCGTCTCAACAGATGAGGCAACGTTGGACGTGGAAGATATTGGTGCACAAGTAGAGAGAAGTTTTGAATCAAAAGAGGACTTCGAGGAGAAAATCCCAACGGAGGATGAAACGTTCTTGAAGGAAGCGGAAGCAGAAACCAAGGAGCATATAAAATGCAGTGATATTGCTCCGGAAGAGAAGGGTCTAGCAGACATCTTAACAGATGAAACATCCTTGGACGTCAAAGATATTGAGGTAAAAGCAGAGGAAAGTTTTGAATCAAATGAGAACCTGGAGGAGCAGATTCAAACAGCAGAAGGGGAAGGTGAAACACGTTTGAGGGAAGCCAAAGCAGAATCTGATGAACGGGTCAAAAATGAGATTGCTCCTGAACAGAAGGTTCTGGAAACCATCTCAAAAGATGAAATGGCCTTGGACATTGATGATGCTTATGTGCAACTGCAAGCAGAGGAAAATTCCGACACAAAAGAGTTCTCGGACAAGGAGAATCCGACAGCAGCAGATGAAGGTGAAGAGAAGATAGCACATGGAAATGTAGTTTTGAATGACACCGCTGGAATGCAAATTCTTGAAGAAGCAGAACCGAAAGTGGGAGTCGAAGAGGAGAGTTGCAAATCAGAAGCTCAACCTGAAAAGACTAGCACGATAGTTGAAGAGGTCTTTGATGAGTCAAAGATCACAGGCAGAGATTTCAACACAGAAGAAATCGTAGAAGGCAATGTCGCATGTTTTACACAAGAATCAATTGGAGAGGATACAGTTAACAAATCCCAAGAAATTGAAACTGAGAATAAAAATTTAAAGGAAGAGGGAAGCTGTATAGAGAGCTTAAAAGAAGGCAGCGGTGATCGATCTCTCACCGGAGTACATTCGAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

2474

Amino Acids

274.16

Weight (kDa)

4.1

Isoelectric Point (pI)

53.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 89
AatII GACGTC 2 cut(s) 2340, 6762
Acc36I ACCTGC 2 cut(s) 1034, 2563
Acc65I GGTACC 1 cut(s) 782
AccB1I GGYRCC 1 cut(s) 782
AccI GTMKAC 2 cut(s) 851, 4326
AccII CGCG 4 cut(s) 964, 2987, 4397, 5207
AccIII TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
AclI AACGTT 2 cut(s) 6560, 6650
AclWI GGATC 1 cut(s) 5323
AcyI GRCGYC 2 cut(s) 2337, 6759
AfiI CCNNNNNNNGG 6 cut(s) 796, 3436, 3800, 4846, 6640, 7130
AflIII ACRYGT 9 cut(s) 187, 2908, 3173, 4318, 4583, 5128, 5390, 5897, 6851
AgeI ACCGGT 1 cut(s) 1391
AhdI GACNNNNNGTC 2 cut(s) 1657, 3919
AhlI ACTAGT 2 cut(s) 581, 854
AjiI CACGTC 2 cut(s) 1993, 6568
AjnI CCWGG 5 cut(s) 2634, 3516, 3894, 4926, 6810
AjuI GAANNNNNNNTTGG 2 cut(s) 1167, 1199
AleI CACNNNNGTG 1 cut(s) 830
AloI GAACNNNNNNTCC 4 cut(s) 609, 641, 6635, 6667
Alw21I GWGCWC 6 cut(s) 360, 692, 884, 1531, 1810, 6586
Alw44I GTGCAC 2 cut(s) 1806, 6582
AlwI GGATC 1 cut(s) 5323
AlwNI CAGNNNCTG 2 cut(s) 3866, 6878
Ama87I CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
Aor13HI TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
ApaLI GTGCAC 2 cut(s) 1806, 6582
ArsI GACNNNNNNTTYG 4 cut(s) 3807, 3839, 5999, 6031
AsiGI ACCGGT 1 cut(s) 1391
Asp700I GAANNNNTTC 4 cut(s) 732, 3459, 4869, 5676
Asp718I GGTACC 1 cut(s) 782
AspS9I GGNCC 3 cut(s) 1519, 3358, 3781
AsuC2I CCSGG 2 cut(s) 1003, 1004
AsuII TTCGAA 2 cut(s) 6000, 6161
AvaI CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
AvaII GGWCC 2 cut(s) 1519, 3781
AxyI CCTNAGG 1 cut(s) 978
BaeGI GKGCMC 2 cut(s) 1810, 6586
BaeI ACNNNNGTAYC 2 cut(s) 1992, 2025
BanI GGYRCC 1 cut(s) 782
BanII GRGCYC 3 cut(s) 1449, 3186, 4596
BauI CACGAG 1 cut(s) 1809
BbsI GAAGAC 7 cut(s) 60, 474, 2652, 2982, 4392, 5574, 6126
Bbv12I GWGCWC 6 cut(s) 360, 692, 884, 1531, 1810, 6586
BbvCI CCTCAGC 6 cut(s) 15, 1452, 1584, 1899, 3714, 3846
BceAI ACGGC 2 cut(s) 57, 5619
BcgI CGANNNNNNTGC 2 cut(s) 465, 499
BciT130I CCWGG 5 cut(s) 2636, 3518, 3896, 4928, 6812
BciVI GTATCC 2 cut(s) 43, 7288
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 2 cut(s) 1003, 1004
BcuI ACTAGT 2 cut(s) 581, 854
BfmI CTRYAG 3 cut(s) 351, 5250, 5682
BfuAI ACCTGC 2 cut(s) 1034, 2563
BfuI GTATCC 2 cut(s) 43, 7288
BglII AGATCT 2 cut(s) 722, 3553
BmcAI AGTACT 1 cut(s) 2434
Bme1390I CCNGG 7 cut(s) 1003, 1004, 2636, 3518, 3896, 4928, 6812
Bme18I GGWCC 2 cut(s) 1519, 3781
BmeRI GACNNNNNGTC 2 cut(s) 1657, 3919
BmeT110I CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
BmgBI CACGTC 2 cut(s) 1993, 6568
BmgT120I GGNCC 3 cut(s) 1519, 3358, 3781
BmiI GGNNCC 4 cut(s) 21, 702, 784, 5160
BmrFI CCNGG 7 cut(s) 1003, 1004, 2636, 3518, 3896, 4928, 6812
BmrI ACTGGG 3 cut(s) 101, 623, 4590
BmuI ACTGGG 3 cut(s) 101, 623, 4590
BoxI GACNNNNGTC 2 cut(s) 5574, 6126
BpiI GAAGAC 7 cut(s) 60, 474, 2652, 2982, 4392, 5574, 6126
BpmI CTGGAG 7 cut(s) 2922, 3987, 4332, 5142, 5505, 6501, 6833
Bpu10I CCTNAGC 7 cut(s) 15, 1365, 1452, 1584, 1899, 3714, 3846
Bpu14I TTCGAA 2 cut(s) 6000, 6161
BpuEI CTTGAG 7 cut(s) 1163, 1194, 2894, 3452, 4304, 4862, 5114
BpuMI CCSGG 2 cut(s) 1003, 1004
Bsa29I ATCGAT 1 cut(s) 7390
BsaHI GRCGYC 2 cut(s) 2337, 6759
BsaI GGTCTC 1 cut(s) 4847
Bsc4I CCNNNNNNNGG 6 cut(s) 796, 3436, 3800, 4846, 6640, 7130
Bse118I RCCGGY 1 cut(s) 1391
Bse1I ACTGG 8 cut(s) 107, 300, 629, 2905, 4293, 4315, 4596, 5125
Bse21I CCTNAGG 1 cut(s) 978
Bse3DI GCAATG 1 cut(s) 7264
BseAI TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
BseBI CCWGG 5 cut(s) 2636, 3518, 3896, 4928, 6812
BseCI ATCGAT 1 cut(s) 7390
BseLI CCNNNNNNNGG 6 cut(s) 796, 3436, 3800, 4846, 6640, 7130
BseMI GCAATG 1 cut(s) 7264
BseNI ACTGG 8 cut(s) 107, 300, 629, 2905, 4293, 4315, 4596, 5125
BseSI GKGCMC 2 cut(s) 1810, 6586
BsgI GTGCAG 4 cut(s) 879, 1012, 3051, 4461
Bsh1236I CGCG 4 cut(s) 964, 2987, 4397, 5207
BshNI GGYRCC 1 cut(s) 782
BshTI ACCGGT 1 cut(s) 1391
BshVI ATCGAT 1 cut(s) 7390
BsiHKAI GWGCWC 6 cut(s) 360, 692, 884, 1531, 1810, 6586
BsiHKCI CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
BslFI GGGAC 2 cut(s) 2559, 2918
BslI CCNNNNNNNGG 6 cut(s) 796, 3436, 3800, 4846, 6640, 7130
BsmBI CGTCTC 4 cut(s) 520, 1589, 1832, 6547
BsmFI GGGAC 2 cut(s) 2559, 2918
BsmI GAATGC 2 cut(s) 1883, 7104
Bso31I GGTCTC 1 cut(s) 4847
BsoBI CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
Bsp119I TTCGAA 2 cut(s) 6000, 6161
Bsp1286I GDGCHC 9 cut(s) 360, 692, 884, 1449, 1531, 1810, 3186, 4596, 6586
Bsp13I TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
Bsp1407I TGTACA 5 cut(s) 2656, 3412, 4069, 4822, 5629
BspDI ATCGAT 1 cut(s) 7390
BspEI TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
BspFNI CGCG 4 cut(s) 964, 2987, 4397, 5207
BspHI TCATGA 4 cut(s) 2446, 3547, 4957, 5764
BspLI GGNNCC 4 cut(s) 21, 702, 784, 5160
BspMAI CTGCAG 1 cut(s) 5254
BspMI ACCTGC 2 cut(s) 1034, 2563
BspPI GGATC 1 cut(s) 5323
BspQI GCTCTTC 2 cut(s) 4566, 6417
BspT104I TTCGAA 2 cut(s) 6000, 6161
BspT107I GGYRCC 1 cut(s) 782
BspTNI GGTCTC 1 cut(s) 4847
BsrDI GCAATG 1 cut(s) 7264
BsrFI RCCGGY 1 cut(s) 1391
BsrGI TGTACA 5 cut(s) 2656, 3412, 4069, 4822, 5629
BsrI ACTGG 8 cut(s) 107, 300, 629, 2905, 4293, 4315, 4596, 5125
BssAI RCCGGY 1 cut(s) 1391
BssNAI GTATAC 2 cut(s) 852, 4327
BssNI GRCGYC 2 cut(s) 2337, 6759
BssSI CACGAG 1 cut(s) 1809
BssT1I CCWWGG 7 cut(s) 1068, 1376, 4857, 5958, 6678, 6753, 6951
Bst1107I GTATAC 2 cut(s) 852, 4327
Bst2BI CACGAG 1 cut(s) 1809
Bst2UI CCWGG 5 cut(s) 2636, 3518, 3896, 4928, 6812
BstACI GRCGYC 2 cut(s) 2337, 6759
BstAUI TGTACA 5 cut(s) 2656, 3412, 4069, 4822, 5629
BstBI TTCGAA 2 cut(s) 6000, 6161
BstC8I GCNNGC 7 cut(s) 441, 1449, 2013, 3168, 3711, 4578, 6984
BstDSI CCRYGG 4 cut(s) 39, 2985, 4395, 5205
BstFNI CGCG 4 cut(s) 964, 2987, 4397, 5207
BstMWI GCNNNNNNNGC 9 cut(s) 11, 1457, 1535, 2246, 3613, 3719, 5023, 5830, 5909
BstNI CCWGG 5 cut(s) 2636, 3518, 3896, 4928, 6812
BstPAI GACNNNNGTC 2 cut(s) 5574, 6126
BstSCI CCNGG 7 cut(s) 1001, 1002, 2634, 3516, 3894, 4926, 6810
BstSFI CTRYAG 3 cut(s) 351, 5250, 5682
BstSLI GKGCMC 2 cut(s) 1810, 6586
BstUI CGCG 4 cut(s) 964, 2987, 4397, 5207
BstV2I GAAGAC 7 cut(s) 60, 474, 2652, 2982, 4392, 5574, 6126
BstX2I RGATCY 3 cut(s) 722, 3553, 5328
BstXI CCANNNNNNTGG 1 cut(s) 832
BstYI RGATCY 3 cut(s) 722, 3553, 5328
BstZ17I GTATAC 2 cut(s) 852, 4327
Bsu15I ATCGAT 1 cut(s) 7390
Bsu36I CCTNAGG 1 cut(s) 978
BsuI GTATCC 2 cut(s) 43, 7288
BsuTUI ATCGAT 1 cut(s) 7390
BtgI CCRYGG 4 cut(s) 39, 2985, 4395, 5205
BtrI CACGTC 2 cut(s) 1993, 6568
BtsI GCAGTG 3 cut(s) 510, 5073, 6703
BveI ACCTGC 2 cut(s) 1034, 2563
Cac8I GCNNGC 7 cut(s) 441, 1449, 2013, 3168, 3711, 4578, 6984
CaiI CAGNNNCTG 2 cut(s) 3866, 6878
CciI TCATGA 4 cut(s) 2446, 3547, 4957, 5764
Cfr10I RCCGGY 1 cut(s) 1391
Cfr13I GGNCC 3 cut(s) 1519, 3358, 3781
Cfr42I CCGCGG 3 cut(s) 2988, 4398, 5208
Cfr9I CCCGGG 1 cut(s) 1002
ClaI ATCGAT 1 cut(s) 7390
CseI GACGC 3 cut(s) 1647, 3909, 5564
CspAI ACCGGT 1 cut(s) 1391
CspCI CAANNNNNGTGG 2 cut(s) 906, 941
DraI TTTAAA 1 cut(s) 7341
DrdI GACNNNNNNGTC 1 cut(s) 89
DriI GACNNNNNGTC 2 cut(s) 1657, 3919
DseDI GACNNNNNNGTC 1 cut(s) 89
Eam1105I GACNNNNNGTC 2 cut(s) 1657, 3919
EciI GGCGGA 4 cut(s) 25, 59, 3603, 5013
Eco130I CCWWGG 7 cut(s) 1068, 1376, 4857, 5958, 6678, 6753, 6951
Eco147I AGGCCT 1 cut(s) 5219
Eco24I GRGCYC 3 cut(s) 1449, 3186, 4596
Eco31I GGTCTC 1 cut(s) 4847
Eco32I GATATC 2 cut(s) 62, 5213
Eco47I GGWCC 2 cut(s) 1519, 3781
Eco81I CCTNAGG 1 cut(s) 978
Eco88I CYCGRG 5 cut(s) 1002, 2946, 4356, 5166, 6205
EcoO109I RGGNCCY 2 cut(s) 1519, 3781
EcoRI GAATTC 3 cut(s) 370, 2788, 4201
EcoRII CCWGG 5 cut(s) 2634, 3516, 3894, 4926, 6810
EcoRV GATATC 2 cut(s) 62, 5213
EcoT14I CCWWGG 7 cut(s) 1068, 1376, 4857, 5958, 6678, 6753, 6951
EcoT38I GRGCYC 3 cut(s) 1449, 3186, 4596
ErhI CCWWGG 7 cut(s) 1068, 1376, 4857, 5958, 6678, 6753, 6951
Esp3I CGTCTC 4 cut(s) 520, 1589, 1832, 6547
FalI AAGNNNNNCTT 4 cut(s) 720, 752, 3414, 3446
FaqI GGGAC 2 cut(s) 2559, 2918
FauI CCCGC 3 cut(s) 957, 1456, 5200
FbaI TGATCA 1 cut(s) 769
FblI GTMKAC 2 cut(s) 851, 4326
FriOI GRGCYC 3 cut(s) 1449, 3186, 4596
GsuI CTGGAG 7 cut(s) 2922, 3987, 4332, 5142, 5505, 6501, 6833
HgaI GACGC 3 cut(s) 1647, 3909, 5564
Hin1I GRCGYC 2 cut(s) 2337, 6759
HincII GTYRAC 6 cut(s) 94, 143, 256, 3583, 4993, 7303
HindII GTYRAC 6 cut(s) 94, 143, 256, 3583, 4993, 7303
HindIII AAGCTT 4 cut(s) 2230, 3640, 5857, 6259
HpaI GTTAAC 1 cut(s) 7303
Hpy99I CGWCG 4 cut(s) 1663, 1994, 2342, 3925
HpyF10VI GCNNNNNNNGC 9 cut(s) 11, 1457, 1535, 2246, 3613, 3719, 5023, 5830, 5909
Hsp92I GRCGYC 2 cut(s) 2337, 6759
Kpn2I TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
KpnI GGTACC 1 cut(s) 786
Ksp22I TGATCA 1 cut(s) 769
KspAI GTTAAC 1 cut(s) 7303
KspI CCGCGG 3 cut(s) 2988, 4398, 5208
LguI GCTCTTC 2 cut(s) 4566, 6417
MfeI CAATTG 4 cut(s) 113, 1533, 6033, 7284
MflI RGATCY 3 cut(s) 722, 3553, 5328
MhlI GDGCHC 9 cut(s) 360, 692, 884, 1449, 1531, 1810, 3186, 4596, 6586
MroI TCCGGA 6 cut(s) 22, 1327, 1741, 3266, 3346, 6709
MroXI GAANNNNTTC 4 cut(s) 732, 3459, 4869, 5676
MslI CAYNNNNRTG 9 cut(s) 830, 2166, 3630, 4962, 5769, 5847, 6344, 6450, 7071
MspR9I CCNGG 7 cut(s) 1003, 1004, 2636, 3518, 3896, 4928, 6812
MunI CAATTG 4 cut(s) 113, 1533, 6033, 7284
Mva1269I GAATGC 2 cut(s) 1883, 7104
MvaI CCWGG 5 cut(s) 2636, 3518, 3896, 4928, 6812
MvnI CGCG 4 cut(s) 964, 2987, 4397, 5207
MwoI GCNNNNNNNGC 9 cut(s) 11, 1457, 1535, 2246, 3613, 3719, 5023, 5830, 5909
NciI CCSGG 2 cut(s) 1003, 1004
NlaIV GGNNCC 4 cut(s) 21, 702, 784, 5160
NmuCI GTSAC 7 cut(s) 427, 758, 862, 2824, 4234, 5041, 5893
NspV TTCGAA 2 cut(s) 6000, 6161
OliI CACNNNNGTG 1 cut(s) 830
PaeI GCATGC 2 cut(s) 3170, 4580
PaeR7I CTCGAG 4 cut(s) 2946, 4356, 5166, 6205
PagI TCATGA 4 cut(s) 2446, 3547, 4957, 5764
PceI AGGCCT 1 cut(s) 5219
PciI ACATGT 7 cut(s) 2908, 3173, 4318, 4583, 5128, 5390, 5897
PciSI GCTCTTC 2 cut(s) 4566, 6417
PcsI WCGNNNNNNNCGW 1 cut(s) 1503
PctI GAATGC 2 cut(s) 1883, 7104
PdmI GAANNNNTTC 4 cut(s) 732, 3459, 4869, 5676
PinAI ACCGGT 1 cut(s) 1391
PpuMI RGGWCCY 2 cut(s) 1519, 3781
PscI ACATGT 7 cut(s) 2908, 3173, 4318, 4583, 5128, 5390, 5897
PshAI GACNNNNGTC 2 cut(s) 5574, 6126
Psp1406I AACGTT 2 cut(s) 6560, 6650
Psp5II RGGWCCY 2 cut(s) 1519, 3781
Psp6I CCWGG 5 cut(s) 2634, 3516, 3894, 4926, 6810
PspGI CCWGG 5 cut(s) 2634, 3516, 3894, 4926, 6810
PspN4I GGNNCC 4 cut(s) 21, 702, 784, 5160
PspPI GGNCC 3 cut(s) 1519, 3358, 3781
PspPPI RGGWCCY 2 cut(s) 1519, 3781
PspXI VCTCGAGB 1 cut(s) 6205
PsrI GAACNNNNNNTAC 4 cut(s) 2898, 2930, 5118, 5150
PstI CTGCAG 1 cut(s) 5254
PstNI CAGNNNCTG 2 cut(s) 3866, 6878
PsuI RGATCY 3 cut(s) 722, 3553, 5328
PvuII CAGCTG 4 cut(s) 3014, 4424, 5234, 6041
RseI CAYNNNNRTG 9 cut(s) 830, 2166, 3630, 4962, 5769, 5847, 6344, 6450, 7071
SacII CCGCGG 3 cut(s) 2988, 4398, 5208
SapI GCTCTTC 2 cut(s) 4566, 6417
Sau96I GGNCC 3 cut(s) 1519, 3358, 3781
ScaI AGTACT 1 cut(s) 2434
ScrFI CCNGG 7 cut(s) 1003, 1004, 2636, 3518, 3896, 4928, 6812
SduI GDGCHC 9 cut(s) 360, 692, 884, 1449, 1531, 1810, 3186, 4596, 6586
SfcI CTRYAG 3 cut(s) 351, 5250, 5682
Sfr274I CTCGAG 4 cut(s) 2946, 4356, 5166, 6205
Sfr303I CCGCGG 3 cut(s) 2988, 4398, 5208
SfuI TTCGAA 2 cut(s) 6000, 6161
SgrBI CCGCGG 3 cut(s) 2988, 4398, 5208
SinI GGWCC 2 cut(s) 1519, 3781
SlaI CTCGAG 4 cut(s) 2946, 4356, 5166, 6205
SmaI CCCGGG 1 cut(s) 1004
SmiMI CAYNNNNRTG 9 cut(s) 830, 2166, 3630, 4962, 5769, 5847, 6344, 6450, 7071
SpeI ACTAGT 2 cut(s) 581, 854
SphI GCATGC 2 cut(s) 3170, 4580
SseBI AGGCCT 1 cut(s) 5219
SspI AATATT 6 cut(s) 1570, 2056, 3220, 3832, 4630, 5437
StuI AGGCCT 1 cut(s) 5219
StyD4I CCNGG 7 cut(s) 1001, 1002, 2634, 3516, 3894, 4926, 6810
StyI CCWWGG 7 cut(s) 1068, 1376, 4857, 5958, 6678, 6753, 6951
TaqII GACCGA 1 cut(s) 463
TseFI GTSAC 7 cut(s) 427, 758, 862, 2824, 4234, 5041, 5893
Tsp45I GTSAC 7 cut(s) 427, 758, 862, 2824, 4234, 5041, 5893
TspMI CCCGGG 1 cut(s) 1002
VneI GTGCAC 2 cut(s) 1806, 6582
VpaK11BI GGWCC 2 cut(s) 1519, 3781
XbaI TCTAGA 3 cut(s) 3142, 4552, 5362
XhoI CTCGAG 4 cut(s) 2946, 4356, 5166, 6205
XmaI CCCGGG 1 cut(s) 1002
XmiI GTMKAC 2 cut(s) 851, 4326
XmnI GAANNNNTTC 4 cut(s) 732, 3459, 4869, 5676
ZraI GACGTC 2 cut(s) 2338, 6760
ZrmI AGTACT 1 cut(s) 2434
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.