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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 4 cut(s) | 4244, 6130, 7972, 13055 |
| AasI | GACNNNNNNGTC | 2 cut(s) | 4199, 8326 |
| Acc65I | GGTACC | 2 cut(s) | 8923, 10372 |
| AccB1I | GGYRCC | 4 cut(s) | 7087, 8923, 10372, 13588 |
| AccBSI | CCGCTC | 1 cut(s) | 3638 |
| AccI | GTMKAC | 9 cut(s) | 57, 96, 2977, 7650, 8037, 9486, 10932, 12290, 12357 |
| AccII | CGCG | 2 cut(s) | 41, 6647 |
| AciI | CCGC | 4 cut(s) | 3638, 6645, 8894, 13400 |
| AclWI | GGATC | 8 cut(s) | 150, 1929, 3393, 11167, 11338, 12324, 14690, 14703 |
| AcoI | YGGCCR | 1 cut(s) | 295 |
| AcvI | CACGTG | 2 cut(s) | 2253, 5533 |
| AcyI | GRCGYC | 1 cut(s) | 956 |
| AdeI | CACNNNGTG | 2 cut(s) | 12617, 13588 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 277, 781, 1622, 3491, 4648, 5134, 6477, 7775, 12620 |
| AflII | CTTAAG | 2 cut(s) | 3985, 4186 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 5380, 14632 |
| AhlI | ACTAGT | 3 cut(s) | 5887, 11534, 12136 |
| AjiI | CACGTC | 2 cut(s) | 9004, 10453 |
| AjnI | CCWGG | 4 cut(s) | 4002, 10317, 11523, 14669 |
| AleI | CACNNNNGTG | 2 cut(s) | 3503, 10069 |
| Alw44I | GTGCAC | 2 cut(s) | 1358, 12925 |
| AlwI | GGATC | 8 cut(s) | 150, 1929, 3393, 11167, 11338, 12324, 14690, 14703 |
| AlwNI | CAGNNNCTG | 4 cut(s) | 389, 2099, 3113, 14453 |
| Ama87I | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| ApaI | GGGCCC | 1 cut(s) | 4003 |
| ApaLI | GTGCAC | 2 cut(s) | 1358, 12925 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 158, 190, 4677, 4709 |
| AseI | ATTAAT | 3 cut(s) | 3345, 12122, 13683 |
| Asp718I | GGTACC | 2 cut(s) | 8923, 10372 |
| AspLEI | GCGC | 2 cut(s) | 340, 14452 |
| AsuC2I | CCSGG | 3 cut(s) | 11979, 11980, 12254 |
| AsuII | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| AvaI | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| AvaII | GGWCC | 5 cut(s) | 268, 4006, 6211, 6359, 12719 |
| AxyI | CCTNAGG | 3 cut(s) | 12189, 13995, 14337 |
| BaeGI | GKGCMC | 3 cut(s) | 1362, 4003, 12929 |
| BamHI | GGATCC | 1 cut(s) | 14695 |
| BanI | GGYRCC | 4 cut(s) | 7087, 8923, 10372, 13588 |
| BarI | GAAGNNNNNNTAC | 6 cut(s) | 327, 359, 682, 714, 6333, 6365 |
| BauI | CACGAG | 2 cut(s) | 4884, 9931 |
| BbrPI | CACGTG | 2 cut(s) | 2253, 5533 |
| BbvCI | CCTCAGC | 1 cut(s) | 11454 |
| BceAI | ACGGC | 2 cut(s) | 2166, 3018 |
| BciT130I | CCWGG | 4 cut(s) | 4004, 10319, 11525, 14671 |
| BciVI | GTATCC | 3 cut(s) | 5626, 8401, 11290 |
| BcnI | CCSGG | 3 cut(s) | 11979, 11980, 12254 |
| BcuI | ACTAGT | 3 cut(s) | 5887, 11534, 12136 |
| BfoI | RGCGCY | 1 cut(s) | 14453 |
| BfrI | CTTAAG | 2 cut(s) | 3985, 4186 |
| BfuI | GTATCC | 3 cut(s) | 5626, 8401, 11290 |
| BglII | AGATCT | 7 cut(s) | 5276, 6923, 8759, 9664, 10208, 13109, 13255 |
| Bme1390I | CCNGG | 7 cut(s) | 4004, 10319, 11525, 11979, 11980, 12254, 14671 |
| Bme18I | GGWCC | 5 cut(s) | 268, 4006, 6211, 6359, 12719 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 5380, 14632 |
| BmeT110I | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| BmgBI | CACGTC | 2 cut(s) | 9004, 10453 |
| BmrFI | CCNGG | 7 cut(s) | 4004, 10319, 11525, 11979, 11980, 12254, 14671 |
| BmrI | ACTGGG | 1 cut(s) | 11516 |
| BmuI | ACTGGG | 1 cut(s) | 11516 |
| Bpu10I | CCTNAGC | 2 cut(s) | 11454, 12882 |
| Bpu14I | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| BpuMI | CCSGG | 3 cut(s) | 11979, 11980, 12254 |
| Bsa29I | ATCGAT | 2 cut(s) | 2172, 3420 |
| BsaAI | YACGTR | 2 cut(s) | 2253, 5533 |
| BsaBI | GATNNNNATC | 5 cut(s) | 147, 825, 7764, 11043, 14694 |
| BsaHI | GRCGYC | 1 cut(s) | 956 |
| BsaWI | WCCGGW | 1 cut(s) | 5695 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 277, 781, 1622, 3491, 4648, 5134, 6477, 7775, 12620 |
| Bse118I | RCCGGY | 1 cut(s) | 293 |
| Bse21I | CCTNAGG | 3 cut(s) | 12189, 13995, 14337 |
| Bse8I | GATNNNNATC | 5 cut(s) | 147, 825, 7764, 11043, 14694 |
| BseBI | CCWGG | 4 cut(s) | 4004, 10319, 11525, 14671 |
| BseCI | ATCGAT | 2 cut(s) | 2172, 3420 |
| BseJI | GATNNNNATC | 5 cut(s) | 147, 825, 7764, 11043, 14694 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 277, 781, 1622, 3491, 4648, 5134, 6477, 7775, 12620 |
| BseSI | GKGCMC | 3 cut(s) | 1362, 4003, 12929 |
| BseYI | CCCAGC | 1 cut(s) | 5344 |
| Bsh1236I | CGCG | 2 cut(s) | 41, 6647 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1758 |
| BshNI | GGYRCC | 4 cut(s) | 7087, 8923, 10372, 13588 |
| BshVI | ATCGAT | 2 cut(s) | 2172, 3420 |
| BsiEI | CGRYCG | 1 cut(s) | 1758 |
| BsiHKCI | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| BsiSI | CCGG | 4 cut(s) | 294, 5696, 11979, 12254 |
| BslFI | GGGAC | 4 cut(s) | 1740, 8584, 10033, 14735 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 277, 781, 1622, 3491, 4648, 5134, 6477, 7775, 12620 |
| BsmBI | CGTCTC | 5 cut(s) | 1745, 4185, 8994, 10443, 14639 |
| BsmFI | GGGAC | 4 cut(s) | 1740, 8584, 10033, 14735 |
| BsmI | GAATGC | 9 cut(s) | 997, 4621, 6599, 8076, 8435, 10074, 11315, 13004, 14652 |
| BsoBI | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| Bsp119I | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| Bsp120I | GGGCCC | 1 cut(s) | 3999 |
| Bsp68I | TCGCGA | 1 cut(s) | 41 |
| BspACI | CCGC | 4 cut(s) | 3638, 6645, 8894, 13400 |
| BspDI | ATCGAT | 2 cut(s) | 2172, 3420 |
| BspFNI | CGCG | 2 cut(s) | 41, 6647 |
| BspHI | TCATGA | 4 cut(s) | 3559, 4363, 7834, 13222 |
| BspMAI | CTGCAG | 5 cut(s) | 469, 1150, 2038, 5594, 5725 |
| BspPI | GGATC | 8 cut(s) | 150, 1929, 3393, 11167, 11338, 12324, 14690, 14703 |
| BspT104I | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| BspT107I | GGYRCC | 4 cut(s) | 7087, 8923, 10372, 13588 |
| BspTI | CTTAAG | 2 cut(s) | 3985, 4186 |
| BsrBI | CCGCTC | 1 cut(s) | 3638 |
| BsrFI | RCCGGY | 1 cut(s) | 293 |
| BssAI | RCCGGY | 1 cut(s) | 293 |
| BssNAI | GTATAC | 1 cut(s) | 12291 |
| BssNI | GRCGYC | 1 cut(s) | 956 |
| BssSI | CACGAG | 2 cut(s) | 4884, 9931 |
| Bst1107I | GTATAC | 1 cut(s) | 12291 |
| Bst2BI | CACGAG | 2 cut(s) | 4884, 9931 |
| Bst2UI | CCWGG | 4 cut(s) | 4004, 10319, 11525, 14671 |
| BstACI | GRCGYC | 1 cut(s) | 956 |
| BstAFI | CTTAAG | 2 cut(s) | 3985, 4186 |
| BstAPI | GCANNNNNTGC | 6 cut(s) | 907, 1154, 1907, 7871, 8736, 11150 |
| BstBAI | YACGTR | 2 cut(s) | 2253, 5533 |
| BstBI | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| BstDSI | CCRYGG | 3 cut(s) | 2784, 4335, 10869 |
| BstEII | GGTNACC | 3 cut(s) | 2491, 3337, 4330 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 779 |
| BstFNI | CGCG | 2 cut(s) | 41, 6647 |
| BstH2I | RGCGCY | 1 cut(s) | 14453 |
| BstHHI | GCGC | 2 cut(s) | 340, 14452 |
| BstMCI | CGRYCG | 1 cut(s) | 1758 |
| BstNI | CCWGG | 4 cut(s) | 4004, 10319, 11525, 14671 |
| BstPI | GGTNACC | 3 cut(s) | 2491, 3337, 4330 |
| BstSCI | CCNGG | 7 cut(s) | 4002, 10317, 11523, 11977, 11978, 12252, 14669 |
| BstSLI | GKGCMC | 3 cut(s) | 1362, 4003, 12929 |
| BstUI | CGCG | 2 cut(s) | 41, 6647 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 12044, 14033 |
| BstZ17I | GTATAC | 1 cut(s) | 12291 |
| Bsu15I | ATCGAT | 2 cut(s) | 2172, 3420 |
| Bsu36I | CCTNAGG | 3 cut(s) | 12189, 13995, 14337 |
| BsuI | GTATCC | 3 cut(s) | 5626, 8401, 11290 |
| BsuTUI | ATCGAT | 2 cut(s) | 2172, 3420 |
| BtgI | CCRYGG | 3 cut(s) | 2784, 4335, 10869 |
| BtgZI | GCGATG | 1 cut(s) | 13743 |
| BtrI | CACGTC | 2 cut(s) | 9004, 10453 |
| BtsI | GCAGTG | 3 cut(s) | 5718, 7656, 13152 |
| BtuMI | TCGCGA | 1 cut(s) | 41 |
| CaiI | CAGNNNCTG | 4 cut(s) | 389, 2099, 3113, 14453 |
| CciI | TCATGA | 4 cut(s) | 3559, 4363, 7834, 13222 |
| CfoI | GCGC | 2 cut(s) | 340, 14452 |
| Cfr10I | RCCGGY | 1 cut(s) | 293 |
| Cfr9I | CCCGGG | 1 cut(s) | 11978 |
| ClaI | ATCGAT | 2 cut(s) | 2172, 3420 |
| CseI | GACGC | 3 cut(s) | 964, 7292, 14081 |
| DraI | TTTAAA | 2 cut(s) | 2856, 13096 |
| DraIII | CACNNNGTG | 2 cut(s) | 12617, 13588 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 4199, 8326 |
| DriI | GACNNNNNGTC | 2 cut(s) | 5380, 14632 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 4199, 8326 |
| EaeI | YGGCCR | 1 cut(s) | 295 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 5380, 14632 |
| Ecl136II | GAGCTC | 6 cut(s) | 2211, 4557, 7467, 12899, 14011, 14173 |
| Eco147I | AGGCCT | 1 cut(s) | 12113 |
| Eco32I | GATATC | 4 cut(s) | 2170, 3301, 3418, 11778 |
| Eco47I | GGWCC | 5 cut(s) | 268, 4006, 6211, 6359, 12719 |
| Eco53kI | GAGCTC | 6 cut(s) | 2211, 4557, 7467, 12899, 14011, 14173 |
| Eco72I | CACGTG | 2 cut(s) | 2253, 5533 |
| Eco81I | CCTNAGG | 3 cut(s) | 12189, 13995, 14337 |
| Eco88I | CYCGRG | 5 cut(s) | 3649, 5811, 11978, 12305, 12851 |
| Eco91I | GGTNACC | 3 cut(s) | 2491, 3337, 4330 |
| EcoICRI | GAGCTC | 6 cut(s) | 2211, 4557, 7467, 12899, 14011, 14173 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 779 |
| EcoO109I | RGGNCCY | 3 cut(s) | 3999, 4000, 11786 |
| EcoO65I | GGTNACC | 3 cut(s) | 2491, 3337, 4330 |
| EcoRI | GAATTC | 2 cut(s) | 4278, 13418 |
| EcoRII | CCWGG | 4 cut(s) | 4002, 10317, 11523, 14669 |
| EcoRV | GATATC | 4 cut(s) | 2170, 3301, 3418, 11778 |
| EcoT22I | ATGCAT | 5 cut(s) | 1159, 6599, 6838, 8435, 9884 |
| Esp3I | CGTCTC | 5 cut(s) | 1745, 4185, 8994, 10443, 14639 |
| FaqI | GGGAC | 4 cut(s) | 1740, 8584, 10033, 14735 |
| FblI | GTMKAC | 9 cut(s) | 57, 96, 2977, 7650, 8037, 9486, 10932, 12290, 12357 |
| GlaI | GCGC | 2 cut(s) | 339, 14451 |
| GsaI | CCCAGC | 1 cut(s) | 5348 |
| HaeII | RGCGCY | 1 cut(s) | 14453 |
| HapII | CCGG | 4 cut(s) | 294, 5696, 11979, 12254 |
| HgaI | GACGC | 3 cut(s) | 964, 7292, 14081 |
| HhaI | GCGC | 2 cut(s) | 340, 14452 |
| Hin1I | GRCGYC | 1 cut(s) | 956 |
| Hin6I | GCGC | 2 cut(s) | 338, 14450 |
| HinP1I | GCGC | 2 cut(s) | 338, 14450 |
| HincII | GTYRAC | 4 cut(s) | 97, 1705, 3244, 10198 |
| HindII | GTYRAC | 4 cut(s) | 97, 1705, 3244, 10198 |
| HpaI | GTTAAC | 1 cut(s) | 10198 |
| HpaII | CCGG | 4 cut(s) | 294, 5696, 11979, 12254 |
| Hpy99I | CGWCG | 3 cut(s) | 6416, 7922, 10205 |
| HpyCH4IV | ACGT | 9 cut(s) | 2252, 3097, 4017, 5532, 6411, 9003, 10200, 10452, 14682 |
| HpySE526I | ACGT | 9 cut(s) | 2252, 3097, 4017, 5532, 6411, 9003, 10200, 10452, 14682 |
| Hsp92I | GRCGYC | 1 cut(s) | 956 |
| HspAI | GCGC | 2 cut(s) | 338, 14450 |
| KpnI | GGTACC | 2 cut(s) | 8927, 10376 |
| KroI | GCCGGC | 1 cut(s) | 293 |
| KroNI | GCCGGC | 1 cut(s) | 295 |
| KspAI | GTTAAC | 1 cut(s) | 10198 |
| MaeII | ACGT | 9 cut(s) | 2252, 3097, 4017, 5532, 6411, 9003, 10200, 10452, 14682 |
| MbiI | CCGCTC | 1 cut(s) | 3638 |
| MfeI | CAATTG | 7 cut(s) | 4533, 5907, 8271, 9720, 9951, 11466, 14862 |
| Mph1103I | ATGCAT | 5 cut(s) | 1159, 6599, 6838, 8435, 9884 |
| MroNI | GCCGGC | 1 cut(s) | 293 |
| MslI | CAYNNNNRTG | 3 cut(s) | 3503, 10069, 14295 |
| MspA1I | CMGCKG | 9 cut(s) | 2033, 3683, 3881, 4605, 5762, 7316, 10601, 12404, 12950 |
| MspCI | CTTAAG | 2 cut(s) | 3985, 4186 |
| MspI | CCGG | 4 cut(s) | 294, 5696, 11979, 12254 |
| MspR9I | CCNGG | 7 cut(s) | 4004, 10319, 11525, 11979, 11980, 12254, 14671 |
| MunI | CAATTG | 7 cut(s) | 4533, 5907, 8271, 9720, 9951, 11466, 14862 |
| Mva1269I | GAATGC | 9 cut(s) | 997, 4621, 6599, 8076, 8435, 10074, 11315, 13004, 14652 |
| MvaI | CCWGG | 4 cut(s) | 4004, 10319, 11525, 14671 |
| MvnI | CGCG | 2 cut(s) | 41, 6647 |
| NaeI | GCCGGC | 1 cut(s) | 295 |
| NciI | CCSGG | 3 cut(s) | 11979, 11980, 12254 |
| NgoMIV | GCCGGC | 1 cut(s) | 293 |
| NmeAIII | GCCGAG | 2 cut(s) | 6496, 13044 |
| NruI | TCGCGA | 1 cut(s) | 41 |
| NsiI | ATGCAT | 5 cut(s) | 1159, 6599, 6838, 8435, 9884 |
| NspV | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| OliI | CACNNNNGTG | 2 cut(s) | 3503, 10069 |
| PaeI | GCATGC | 3 cut(s) | 3230, 11522, 11843 |
| PaeR7I | CTCGAG | 1 cut(s) | 5811 |
| PagI | TCATGA | 4 cut(s) | 3559, 4363, 7834, 13222 |
| PaqCI | CACCTGC | 4 cut(s) | 4244, 6130, 7972, 13055 |
| PasI | CCCWGGG | 1 cut(s) | 14670 |
| PceI | AGGCCT | 1 cut(s) | 12113 |
| PciI | ACATGT | 9 cut(s) | 559, 4754, 5003, 5252, 6899, 8573, 10022, 10184, 11912 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 1764, 4200 |
| PctI | GAATGC | 9 cut(s) | 997, 4621, 6599, 8076, 8435, 10074, 11315, 13004, 14652 |
| PdiI | GCCGGC | 1 cut(s) | 295 |
| PflFI | GACNNNGTC | 1 cut(s) | 3014 |
| PfoI | TCCNGGA | 2 cut(s) | 10317, 12252 |
| PmaCI | CACGTG | 2 cut(s) | 2253, 5533 |
| PmlI | CACGTG | 2 cut(s) | 2253, 5533 |
| Ppu21I | YACGTR | 2 cut(s) | 2253, 5533 |
| PscI | ACATGT | 9 cut(s) | 559, 4754, 5003, 5252, 6899, 8573, 10022, 10184, 11912 |
| PshBI | ATTAAT | 3 cut(s) | 3345, 12122, 13683 |
| Psp124BI | GAGCTC | 6 cut(s) | 2213, 4559, 7469, 12901, 14013, 14175 |
| Psp6I | CCWGG | 4 cut(s) | 4002, 10317, 11523, 14669 |
| PspCI | CACGTG | 2 cut(s) | 2253, 5533 |
| PspEI | GGTNACC | 3 cut(s) | 2491, 3337, 4330 |
| PspFI | CCCAGC | 1 cut(s) | 5344 |
| PspGI | CCWGG | 4 cut(s) | 4002, 10317, 11523, 14669 |
| PspOMI | GGGCCC | 1 cut(s) | 3999 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1143, 1175 |
| PstI | CTGCAG | 5 cut(s) | 469, 1150, 2038, 5594, 5725 |
| PstNI | CAGNNNCTG | 4 cut(s) | 389, 2099, 3113, 14453 |
| PsyI | GACNNNGTC | 1 cut(s) | 3014 |
| PvuII | CAGCTG | 9 cut(s) | 2033, 3683, 3881, 4605, 5762, 7316, 10601, 12404, 12950 |
| RruI | TCGCGA | 1 cut(s) | 41 |
| RseI | CAYNNNNRTG | 3 cut(s) | 3503, 10069, 14295 |
| SacI | GAGCTC | 6 cut(s) | 2213, 4559, 7469, 12901, 14013, 14175 |
| SalI | GTCGAC | 1 cut(s) | 95 |
| ScrFI | CCNGG | 7 cut(s) | 4004, 10319, 11525, 11979, 11980, 12254, 14671 |
| Sfr274I | CTCGAG | 1 cut(s) | 5811 |
| SfuI | TTCGAA | 3 cut(s) | 6995, 8831, 10280 |
| SinI | GGWCC | 5 cut(s) | 268, 4006, 6211, 6359, 12719 |
| SlaI | CTCGAG | 1 cut(s) | 5811 |
| SmaI | CCCGGG | 1 cut(s) | 11980 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 3503, 10069, 14295 |
| SpeI | ACTAGT | 3 cut(s) | 5887, 11534, 12136 |
| SphI | GCATGC | 3 cut(s) | 3230, 11522, 11843 |
| SseBI | AGGCCT | 1 cut(s) | 12113 |
| SsiI | CCGC | 4 cut(s) | 3638, 6645, 8894, 13400 |
| SspI | AATATT | 4 cut(s) | 1495, 7158, 8994, 10443 |
| SstI | GAGCTC | 6 cut(s) | 2213, 4559, 7469, 12901, 14013, 14175 |
| StuI | AGGCCT | 1 cut(s) | 12113 |
| StyD4I | CCNGG | 7 cut(s) | 4002, 10317, 11523, 11977, 11978, 12252, 14669 |
| TaiI | ACGT | 9 cut(s) | 2255, 3100, 4020, 5535, 6414, 9006, 10203, 10455, 14685 |
| TauI | GCSGC | 1 cut(s) | 6647 |
| TspMI | CCCGGG | 1 cut(s) | 11978 |
| Tth111I | GACNNNGTC | 1 cut(s) | 3014 |
| Vha464I | CTTAAG | 2 cut(s) | 3985, 4186 |
| VneI | GTGCAC | 2 cut(s) | 1358, 12925 |
| VpaK11BI | GGWCC | 5 cut(s) | 268, 4006, 6211, 6359, 12719 |
| VspI | ATTAAT | 3 cut(s) | 3345, 12122, 13683 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 779 |
| XbaI | TCTAGA | 4 cut(s) | 2125, 11641, 13106, 13258 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 136 |
| XhoI | CTCGAG | 1 cut(s) | 5811 |
| XmaI | CCCGGG | 1 cut(s) | 11978 |
| XmiI | GTMKAC | 9 cut(s) | 57, 96, 2977, 7650, 8037, 9486, 10932, 12290, 12357 |
| Zsp2I | ATGCAT | 5 cut(s) | 1159, 6599, 6838, 8435, 9884 |