MD09G1080800.v1.1

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001480
Physical Location & Seq
Forward (+)
5667208 .. 5668794
1587 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1080800.v1.1.491

Sequence Viewer

Length: 1176 bp
ATGTATGTGACAAGGCCTCTTTCCATGTACAGGAAATCGCCAAGCACCCTTTCCCTTCATCCACCCGATGCTCCACATTCAGGTTTCATGGTGATTACAGATGAAGATGCAGAAGCACACGACGCGTCTTGCTGGGGCCTATGCAAGCGCAGAAAGGTATCGAAACTGCCGTTCCCACAGGACAAGATACTGACCATCGTTTACACGTCGGAGTTCCAAGAGGCCACGAAGACTAAGGTCTGGTTCATCCCGGTTCTTGATCAGCCTCTGTCGTCTCATTGCTACTATGTCATCAAAGCAAGTGGCAGGCACAAAGGGAAAGCTTACAGATGTGCTAGAGAGAGGGACACCGTGAAGTGCTGCTTTAACGACTTCTTGGGAGACAAGAAACCAAGGGTTTTAAATTTTAGAGACATATACCAACAAGTTAAGATTCATCGCTTCGAAGGTGGTGGGTTTTTCGCAAGGGCTGTAGCTCCCGACGGCGTTCCCCCAAAATTCCTCAAGAAAAAAGGATGGAAAGTTCGCAGCTCGAGTATGTATCGTTCACAACTAGGTGAAGCTTTAGGCCTTGACGCCTCGCTCCGTTCACGTCTTCCGGACTTCAATGTTACCATCTTTCAAAAGCGCTCGCGTACTAGCACTGTGGGGAACTGGTACTGTCCATTTGTGTTTGTAAGAGAGAGAGCAACCATAAGACAGCAGATGAAGAAGTCGAAGTACTACAGAGTGACTTTTGAGCAATGGTGGGAAGAGATATACTCTTGTGGGAACGTTAACCGTGAAGGGAACGTTGTGAATGTGAGCGTAGATGTGCAAAGGGAAGTGGCTATGGTATCTGCTATGCAAGCCGTGAAGGATTGTAGGAACGGACGAACAGGGTTCACCTGGTACAAAGCCCATAACCCATACAGCAAGAAAGTGGTCAGTGTAGGTTTGAGCTCCGCAATTGTTCAGAATATGAGATGGGTTTTGGAGGCAGGAGGGTGGGTTAGTGGGGGTGATGAAATGGATGTGAGAGTAGAGAAGGCAGAAGAGATTACAAGTGCAACTCGGTGGAGGAAGTTTGGGTGTTATGTGTTGGTGGAGAGCTTCTCTTTGAAGAGAATGGATGGAAGCTTTGTTTGGAGATCCGATTTTAGGCACACCGATAAGATTCGATGTAAATGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

392

Amino Acids

45.23

Weight (kDa)

9.7

Isoelectric Point (pI)

38.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1262 PF06880 23 - 122 4.2e-40 Protein of unknown function (DUF1262)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 125, 634
AccIII TCCGGA 1 cut(s) 598
AciI CCGC 1 cut(s) 945
AclI AACGTT 2 cut(s) 774, 792
AclWI GGATC 1 cut(s) 1125
AcsI RAATTY 2 cut(s) 403, 497
AcyI GRCGYC 1 cut(s) 576
AfaI GTAC 5 cut(s) 29, 637, 659, 722, 893
AfeI AGCGCT 1 cut(s) 629
AfiI CCNNNNNNNGG 3 cut(s) 30, 80, 1140
AflIII ACRYGT 2 cut(s) 123, 204
AgsI TTSAA 3 cut(s) 607, 623, 1102
AjiI CACGTC 2 cut(s) 207, 593
AjnI CCWGG 1 cut(s) 887
AjuI GAANNNNNNNTTGG 4 cut(s) 34, 66, 1108, 1140
AloI GAACNNNNNNTCC 2 cut(s) 507, 539
AluBI AGCT 7 cut(s) 323, 476, 531, 563, 942, 1092, 1119
AluI AGCT 7 cut(s) 323, 476, 531, 563, 942, 1092, 1119
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 3 cut(s) 279, 375, 405
AlwI GGATC 1 cut(s) 1125
AlwNI CAGNNNCTG 1 cut(s) 268
Ama87I CYCGRG 1 cut(s) 532
Aor13HI TCCGGA 1 cut(s) 598
Aor51HI AGCGCT 1 cut(s) 629
AoxI GGCC 4 cut(s) 14, 136, 222, 568
ApeKI GCWGC 2 cut(s) 360, 528
ApoI RAATTY 2 cut(s) 403, 497
AspLEI GCGC 2 cut(s) 150, 630
AspS9I GGNCC 1 cut(s) 136
AsuC2I CCSGG 1 cut(s) 251
AsuHPI GGTGA 4 cut(s) 103, 569, 877, 1013
AsuII TTCGAA 1 cut(s) 444
AvaI CYCGRG 1 cut(s) 532
BanII GRGCYC 1 cut(s) 944
BarI GAAGNNNNNNTAC 2 cut(s) 704, 736
BbsI GAAGAC 2 cut(s) 236, 587
Bbv12I GWGCWC 1 cut(s) 944
BbvI GCAGC 2 cut(s) 347, 540
BccI CCATC 5 cut(s) 203, 510, 623, 960, 1106
BceAI ACGGC 3 cut(s) 154, 499, 836
BciT130I CCWGG 1 cut(s) 889
BclI TGATCA 1 cut(s) 259
BcnI CCSGG 1 cut(s) 251
BcoDI GTCTC 3 cut(s) 279, 375, 405
BfaI CTAG 3 cut(s) 336, 554, 639
BfmI CTRYAG 2 cut(s) 471, 724
BfoI RGCGCY 1 cut(s) 631
BisI GCNGC 2 cut(s) 361, 529
BlsI GCNGC 2 cut(s) 362, 530
BmcAI AGTACT 1 cut(s) 722
Bme1390I CCNGG 2 cut(s) 251, 889
BmeT110I CYCGRG 1 cut(s) 532
BmgBI CACGTC 2 cut(s) 207, 593
BmgT120I GGNCC 1 cut(s) 136
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 2 cut(s) 251, 889
BmsI GCATC 2 cut(s) 58, 97
BoxI GACNNNNGTC 1 cut(s) 236
BpiI GAAGAC 2 cut(s) 236, 587
BplI GAGNNNNNCTC 4 cut(s) 746, 778, 1079, 1111
Bpu14I TTCGAA 1 cut(s) 444
BpuEI CTTGAG 1 cut(s) 488
BpuMI CCSGG 1 cut(s) 251
BsaHI GRCGYC 1 cut(s) 576
BsaJI CCNNGG 1 cut(s) 392
BsaWI WCCGGW 1 cut(s) 598
BsaXI ACNNNNNCTCC 2 cut(s) 975, 1005
Bsc4I CCNNNNNNNGG 3 cut(s) 30, 80, 1140
Bse1I ACTGG 1 cut(s) 659
Bse3DI GCAATG 2 cut(s) 277, 749
BseAI TCCGGA 1 cut(s) 598
BseBI CCWGG 1 cut(s) 889
BseDI CCNNGG 1 cut(s) 392
BseGI GGATG 5 cut(s) 58, 246, 521, 1018, 1117
BseLI CCNNNNNNNGG 3 cut(s) 30, 80, 1140
BseMI GCAATG 2 cut(s) 277, 749
BseNI ACTGG 1 cut(s) 659
BseXI GCAGC 2 cut(s) 347, 540
BseYI CCCAGC 1 cut(s) 132
Bsh1236I CGCG 2 cut(s) 125, 634
BshFI GGCC 4 cut(s) 16, 138, 224, 570
BsiHKAI GWGCWC 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 532
BsiSI CCGG 2 cut(s) 251, 599
BslFI GGGAC 1 cut(s) 359
BslI CCNNNNNNNGG 3 cut(s) 30, 80, 1140
BsmAI GTCTC 3 cut(s) 279, 375, 405
BsmBI CGTCTC 1 cut(s) 279
BsmFI GGGAC 1 cut(s) 359
BsnI GGCC 4 cut(s) 16, 138, 224, 570
BsoBI CYCGRG 1 cut(s) 532
Bsp119I TTCGAA 1 cut(s) 444
Bsp1286I GDGCHC 1 cut(s) 944
Bsp13I TCCGGA 1 cut(s) 598
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 259, 1130
BspACI CCGC 1 cut(s) 945
BspANI GGCC 4 cut(s) 16, 138, 224, 570
BspEI TCCGGA 1 cut(s) 598
BspFNI CGCG 2 cut(s) 125, 634
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 1 cut(s) 1125
BspT104I TTCGAA 1 cut(s) 444
BsrDI GCAATG 2 cut(s) 277, 749
BsrGI TGTACA 1 cut(s) 27
BsrI ACTGG 1 cut(s) 659
BssECI CCNNGG 1 cut(s) 392
BssMI GATC 2 cut(s) 259, 1130
BssNI GRCGYC 1 cut(s) 576
BssT1I CCWWGG 1 cut(s) 392
Bst2UI CCWGG 1 cut(s) 889
Bst4CI ACNGT 4 cut(s) 352, 646, 662, 782
Bst6I CTCTTC 3 cut(s) 747, 1029, 1097
BstACI GRCGYC 1 cut(s) 576
BstAUI TGTACA 1 cut(s) 27
BstBI TTCGAA 1 cut(s) 444
BstC8I GCNNGC 4 cut(s) 146, 308, 632, 849
BstDEI CTNAG 1 cut(s) 234
BstF5I GGATG 5 cut(s) 58, 246, 521, 1018, 1117
BstFNI CGCG 2 cut(s) 125, 634
BstH2I RGCGCY 1 cut(s) 631
BstHHI GCGC 2 cut(s) 150, 630
BstKTI GATC 2 cut(s) 262, 1133
BstMAI GTCTC 3 cut(s) 279, 375, 405
BstMBI GATC 2 cut(s) 259, 1130
BstMWI GCNNNNNNNGC 2 cut(s) 122, 848
BstNI CCWGG 1 cut(s) 889
BstPAI GACNNNNGTC 1 cut(s) 236
BstSCI CCNGG 2 cut(s) 249, 887
BstSFI CTRYAG 2 cut(s) 471, 724
BstUI CGCG 2 cut(s) 125, 634
BstV1I GCAGC 2 cut(s) 347, 540
BstV2I GAAGAC 2 cut(s) 236, 587
BstX2I RGATCY 1 cut(s) 1130
BstYI RGATCY 1 cut(s) 1130
BsuRI GGCC 4 cut(s) 16, 138, 224, 570
BtgZI GCGATG 1 cut(s) 422
BtrI CACGTC 2 cut(s) 207, 593
BtsCI GGATG 5 cut(s) 58, 246, 521, 1018, 1117
BtsIMutI CAGTG 2 cut(s) 642, 934
Cac8I GCNNGC 4 cut(s) 146, 308, 632, 849
CaiI CAGNNNCTG 1 cut(s) 268
CfoI GCGC 2 cut(s) 150, 630
Cfr13I GGNCC 1 cut(s) 136
CseI GACGC 3 cut(s) 114, 131, 584
CsiI ACCWGGT 1 cut(s) 887
Csp6I GTAC 5 cut(s) 28, 636, 658, 721, 892
CspCI CAANNNNNGTGG 2 cut(s) 283, 318
CviAII CATG 2 cut(s) 25, 88
CviQI GTAC 5 cut(s) 28, 636, 658, 721, 892
DdeI CTNAG 1 cut(s) 234
DpnI GATC 2 cut(s) 261, 1132
DpnII GATC 2 cut(s) 259, 1130
DraI TTTAAA 1 cut(s) 402
Eam1104I CTCTTC 3 cut(s) 747, 1029, 1097
EarI CTCTTC 3 cut(s) 747, 1029, 1097
Ecl136II GAGCTC 1 cut(s) 942
Eco130I CCWWGG 1 cut(s) 392
Eco147I AGGCCT 2 cut(s) 16, 570
Eco24I GRGCYC 1 cut(s) 944
Eco47III AGCGCT 1 cut(s) 629
Eco53kI GAGCTC 1 cut(s) 942
Eco88I CYCGRG 1 cut(s) 532
EcoICRI GAGCTC 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 136
EcoRII CCWGG 1 cut(s) 887
EcoT14I CCWWGG 1 cut(s) 392
EcoT38I GRGCYC 1 cut(s) 944
ErhI CCWWGG 1 cut(s) 392
Esp3I CGTCTC 1 cut(s) 279
FaeI CATG 2 cut(s) 28, 91
FalI AAGNNNNNCTT 2 cut(s) 347, 379
FaqI GGGAC 1 cut(s) 359
FatI CATG 2 cut(s) 24, 87
FbaI TGATCA 1 cut(s) 259
Fnu4HI GCNGC 2 cut(s) 361, 529
FokI GGATG 5 cut(s) 45, 233, 528, 1025, 1124
FriOI GRGCYC 1 cut(s) 944
Fsp4HI GCNGC 2 cut(s) 361, 529
FspBI CTAG 3 cut(s) 336, 554, 639
GlaI GCGC 2 cut(s) 149, 629
GluI GCNGC 2 cut(s) 361, 529
GsaI CCCAGC 1 cut(s) 136
HaeII RGCGCY 1 cut(s) 631
HaeIII GGCC 4 cut(s) 16, 138, 224, 570
HapII CCGG 2 cut(s) 251, 599
HgaI GACGC 3 cut(s) 114, 131, 584
HhaI GCGC 2 cut(s) 150, 630
Hin1I GRCGYC 1 cut(s) 576
Hin1II CATG 2 cut(s) 28, 91
Hin6I GCGC 2 cut(s) 148, 628
HinP1I GCGC 2 cut(s) 148, 628
HincII GTYRAC 1 cut(s) 778
HindII GTYRAC 1 cut(s) 778
HindIII AAGCTT 3 cut(s) 321, 561, 1117
HinfI GANTC 2 cut(s) 433, 1156
HpaI GTTAAC 1 cut(s) 778
HpaII CCGG 2 cut(s) 251, 599
HphI GGTGA 4 cut(s) 103, 569, 877, 1013
Hpy166II GTNNAC 5 cut(s) 202, 548, 590, 778, 885
Hpy188I TCNGA 3 cut(s) 211, 957, 1135
Hpy188III TCNNGA 4 cut(s) 257, 479, 505, 599
Hpy8I GTNNAC 5 cut(s) 202, 548, 590, 778, 885
Hpy99I CGWCG 3 cut(s) 125, 211, 485
HpyAV CCTTC 5 cut(s) 65, 440, 779, 850, 1021
HpyCH4III ACNGT 4 cut(s) 352, 646, 662, 782
HpyCH4IV ACGT 4 cut(s) 206, 592, 774, 792
HpyCH4V TGCA 5 cut(s) 110, 144, 817, 847, 1049
HpyF10VI GCNNNNNNNGC 2 cut(s) 122, 848
HpyF3I CTNAG 1 cut(s) 234
HpySE526I ACGT 4 cut(s) 206, 592, 774, 792
Hsp92I GRCGYC 1 cut(s) 576
Hsp92II CATG 2 cut(s) 28, 91
HspAI GCGC 2 cut(s) 148, 628
Kpn2I TCCGGA 1 cut(s) 598
Ksp22I TGATCA 1 cut(s) 259
KspAI GTTAAC 1 cut(s) 778
Kzo9I GATC 2 cut(s) 259, 1130
LmnI GCTCC 4 cut(s) 76, 481, 588, 947
Lsp1109I GCAGC 2 cut(s) 347, 540
LweI GCATC 2 cut(s) 58, 97
MabI ACCWGGT 1 cut(s) 887
MaeI CTAG 3 cut(s) 336, 554, 639
MaeII ACGT 4 cut(s) 206, 592, 774, 792
MaeIII GTNAC 3 cut(s) 7, 610, 730
MalI GATC 2 cut(s) 261, 1132
MboI GATC 2 cut(s) 259, 1130
MboII GAAGA 7 cut(s) 116, 241, 587, 721, 764, 1046, 1114
MfeI CAATTG 1 cut(s) 948
MflI RGATCY 1 cut(s) 1130
MhlI GDGCHC 1 cut(s) 944
MluCI AATT 3 cut(s) 403, 497, 948
MluI ACGCGT 1 cut(s) 123
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 9 cut(s) 27, 214, 276, 336, 512, 589, 970, 977, 1053
MroI TCCGGA 1 cut(s) 598
MseI TTAA 4 cut(s) 366, 401, 429, 777
MspI CCGG 2 cut(s) 251, 599
MspR9I CCNGG 2 cut(s) 251, 889
MunI CAATTG 1 cut(s) 948
MvaI CCWGG 1 cut(s) 889
MvnI CGCG 2 cut(s) 125, 634
MwoI GCNNNNNNNGC 2 cut(s) 122, 848
NciI CCSGG 1 cut(s) 251
NdeII GATC 2 cut(s) 259, 1130
NlaIII CATG 2 cut(s) 28, 91
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 2 cut(s) 7, 730
NspV TTCGAA 1 cut(s) 444
PaeR7I CTCGAG 1 cut(s) 532
PceI AGGCCT 2 cut(s) 16, 570
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 2 cut(s) 433, 1156
PkrI GCNGC 2 cut(s) 362, 530
PshAI GACNNNNGTC 1 cut(s) 236
Psp124BI GAGCTC 1 cut(s) 944
Psp1406I AACGTT 2 cut(s) 774, 792
Psp6I CCWGG 1 cut(s) 887
PspFI CCCAGC 1 cut(s) 132
PspGI CCWGG 1 cut(s) 887
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 136
PspXI VCTCGAGB 1 cut(s) 532
PsrI GAACNNNNNNTAC 2 cut(s) 529, 561
PstNI CAGNNNCTG 1 cut(s) 268
PsuI RGATCY 1 cut(s) 1130
RsaI GTAC 5 cut(s) 29, 637, 659, 722, 893
RsaNI GTAC 5 cut(s) 28, 636, 658, 721, 892
SacI GAGCTC 1 cut(s) 944
SaqAI TTAA 4 cut(s) 366, 401, 429, 777
SatI GCNGC 2 cut(s) 361, 529
Sau3AI GATC 2 cut(s) 259, 1130
Sau96I GGNCC 1 cut(s) 136
ScaI AGTACT 1 cut(s) 722
ScrFI CCNGG 2 cut(s) 251, 889
SduI GDGCHC 1 cut(s) 944
SexAI ACCWGGT 1 cut(s) 887
SfaNI GCATC 2 cut(s) 58, 97
SfcI CTRYAG 2 cut(s) 471, 724
Sfr274I CTCGAG 1 cut(s) 532
SfuI TTCGAA 1 cut(s) 444
SlaI CTCGAG 1 cut(s) 532
SmlI CTYRAG 2 cut(s) 503, 532
SmoI CTYRAG 2 cut(s) 503, 532
Sse9I AATT 3 cut(s) 403, 497, 948
SseBI AGGCCT 2 cut(s) 16, 570
SsiI CCGC 1 cut(s) 945
SspMI CTAG 3 cut(s) 336, 554, 639
SstI GAGCTC 1 cut(s) 944
StuI AGGCCT 2 cut(s) 16, 570
StyD4I CCNGG 2 cut(s) 249, 887
StyI CCWWGG 1 cut(s) 392
TaaI ACNGT 4 cut(s) 352, 646, 662, 782
TaiI ACGT 4 cut(s) 209, 595, 777, 795
TaqI TCGA 5 cut(s) 161, 444, 533, 716, 1159
TasI AATT 3 cut(s) 403, 497, 948
TatI WGTACW 2 cut(s) 27, 720
TfiI GAWTC 2 cut(s) 433, 1156
Tru1I TTAA 4 cut(s) 366, 401, 429, 777
Tru9I TTAA 4 cut(s) 366, 401, 429, 777
TscAI CASTG 2 cut(s) 649, 934
TseFI GTSAC 2 cut(s) 7, 730
TseI GCWGC 2 cut(s) 360, 528
Tsp45I GTSAC 2 cut(s) 7, 730
TspDTI ATGAA 7 cut(s) 47, 76, 117, 235, 425, 722, 1020
TspGWI ACGGA 2 cut(s) 575, 885
TspRI CASTG 2 cut(s) 649, 934
XapI RAATTY 2 cut(s) 403, 497
XhoI CTCGAG 1 cut(s) 532
XspI CTAG 3 cut(s) 336, 554, 639
ZrmI AGTACT 1 cut(s) 722
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.