Rorug04G0294800

Protein of unknown function (DUF1262)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001480
Physical Location & Seq
Reverse (-)
46908338 .. 46910038
1701 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0294800.1

Sequence Viewer

Length: 1701 bp
ATGTCTGGCACTCTTCAAACCATTGAATCTTTTAGTTCTAAACGGAGAGGAGTAGGCGAATGTGATCAAGAACTGGGGGACAGCGGTAGTGACAGCGATGATATTGGTGAATTTTCCTTCCAGTCGGAGGCAATGCTTGAATATGATGAGCAAAATGGTTTGAGGGCTTTTGCAAAATTTGTTTGCTCTACACAGGTTCCACCTAAGATGGTTGAAGATGCTGATTTTAGGGAGTTGGCACGTAGGTTTAACCCCTCCATTAACATTAGCGTCAAAATGGTTGAAAGTGAATGCTTGATATTCTACGAGGAAAGCAAATTGAGAATTAAAGAATTCTTGGTGAATTTTGAGGGACAGATTAGCCTCTCTCTGGAGATATTGAAGTACTATAACTACCGTGATTACTTGTGTCTGTCAGCACATTTTATTGAAGGGAATTGGAAAATGAAGAAGTTGGTACTTCACTTCCGCTCTGCTTTGGATCCTGAAGATAAGTCATCTGATAATTTTCATTGTCGAGATTGGGGAATTGTGAAGTTGCTTGAAAATTGGGGTATTAAGGATAAAATATCTACTTTCATTATGACTAGTGGGTATGGCAGTGTGGCTGATTGTTTGGAAAGTCAAATCCAAGGAAACAAGGACATTGCTTTGAATGGTTGTCTGTTTCGTCGGTTTCACGTGCGTTGTTGTGGTGAAATGGTCAGTCTAATGGTGCAGGAAGCATTTGACAAGATTAAAGATATCATCGACAAAGTTCGAGCATTGTCTTCTTCTGCTAAATCCTTACCCTTATGGAATCACACAATTTTTGATCTACAGCAAGCCCTGAAGCTGTGGTCTATGGGAGAATACTCTACAAAAGAAATGACCAAGCAAGATAAACCATGCCCATCTCCTGATGAATGGAAGAAAGTTGAAGGTGTATGCAAAATTGTAGGGAGCATATATGAAGTATCAACTGCATTATTTGAGACAAAGCACCTAACTGCTAATGTTTTTCTGTATCACCTTCATGAGTTTCGCGAAATACTGACCCAAATGACGATGCAGTCAGATGGTTTTATCAGGACAATAGCCGAGGCCATGTTGGAAAGGTTGGCCAAGTATTGGGATGAGATGTTCTTGCTGTTGGCAATTACTGCAGCATTGGATCCTCGGTTTAAGATGAAATATGTAGAATTTGTTTGTTCAAAAGTTAAGGGTGATGCAAACGCACAAGTTTTAGCTGTTTGGGGAGCCATTCAGAAACAGTTTGATGCATATGTGATCCTTTTTCCTGAAAAGGAGACCTTTCCGAGTGATTCATCTTCTTCGGATTCAGATTCAGAAGGGGAATCTCCTATTCCCTGGCAAGTAAATCATATCTTTAGTGTGTTGCAAGACTACCACCAGTGCATTCAATCGAGTAATCAGCCTACGAAAAAATCAGAGTTGGATTACTACTTGGAAGAACCTGTCTTACCATGGAGTCAGAAGTTTAGTGCATTGACTTGGTGGAAAACCACCGGTGCCAAGTATCCTACCCTCTCCAGGATGGCACGCGATTTCTTGTCTATTCCAATCTCTCTTGCAACTTCATATGATGCATTCTATACCGAACCAAGGCCAGTTGATGAACACATGATCCGCTTGAAGCCGGACTTAGCAAATGCATTGATGTGTACACGAAGCTGGTACCGTGCACTGAAAGAGTACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1262

Protein Analysis

566

Amino Acids

65.14

Weight (kDa)

5.38

Isoelectric Point (pI)

43.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
hAT-like_RNase-H PF14372 326 - 422 2.9e-20 hAT-like transposase, RNase-H fold
Dimer_Tnp_hAT PF05699 478 - 560 2.4e-24 hAT family C-terminal dimerisation region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1051
Acc65I GGTACC 1 cut(s) 1677
AccB1I GGYRCC 2 cut(s) 1511, 1677
AccB7I CCANNNNNTGG 1 cut(s) 1110
AccBSI CCGCTC 1 cut(s) 471
AccII CGCG 2 cut(s) 1026, 1545
AciI CCGC 3 cut(s) 84, 469, 1630
AclWI GGATC 6 cut(s) 476, 489, 1148, 1161, 1264, 1621
AcoI YGGCCR 1 cut(s) 1101
AcsI RAATTY 5 cut(s) 110, 176, 332, 343, 1181
AcuI CTGAAG 2 cut(s) 507, 851
AcvI CACGTG 1 cut(s) 682
AfaI GTAC 5 cut(s) 386, 459, 1666, 1679, 1697
AfiI CCNNNNNNNGG 5 cut(s) 127, 370, 1110, 1533, 1605
AgeI ACCGGT 1 cut(s) 1508
AhlI ACTAGT 1 cut(s) 587
AjnI CCWGG 2 cut(s) 1349, 1532
AloI GAACNNNNNNTCC 4 cut(s) 1106, 1138, 1611, 1643
AluBI AGCT 3 cut(s) 835, 1229, 1674
AluI AGCT 3 cut(s) 835, 1229, 1674
Alw21I GWGCWC 1 cut(s) 1687
Alw26I GTCTC 2 cut(s) 968, 1283
Alw44I GTGCAC 1 cut(s) 1683
AlwI GGATC 6 cut(s) 476, 489, 1148, 1161, 1264, 1621
AoxI GGCC 3 cut(s) 1083, 1101, 1607
ApaLI GTGCAC 1 cut(s) 1683
ApeKI GCWGC 1 cut(s) 1145
ApoI RAATTY 5 cut(s) 110, 176, 332, 343, 1181
AsiGI ACCGGT 1 cut(s) 1508
Asp718I GGTACC 1 cut(s) 1677
AsuHPI GGTGA 5 cut(s) 119, 352, 707, 1001, 1217
BaeGI GKGCMC 1 cut(s) 1687
BalI TGGCCA 1 cut(s) 1103
BamHI GGATCC 2 cut(s) 481, 1153
BanI GGYRCC 2 cut(s) 1511, 1677
BbrPI CACGTG 1 cut(s) 682
BbsI GAAGAC 1 cut(s) 762
Bbv12I GWGCWC 1 cut(s) 1687
BbvI GCAGC 1 cut(s) 1157
BccI CCATC 4 cut(s) 202, 901, 1052, 1531
BciT130I CCWGG 2 cut(s) 1351, 1534
BciVI GTATCC 1 cut(s) 1530
BclI TGATCA 1 cut(s) 64
BcoDI GTCTC 2 cut(s) 968, 1283
BcuI ACTAGT 1 cut(s) 587
BfaI CTAG 1 cut(s) 588
BfmI CTRYAG 2 cut(s) 818, 1143
BfuI GTATCC 1 cut(s) 1530
BisI GCNGC 1 cut(s) 1146
BlsI GCNGC 1 cut(s) 1147
BmcAI AGTACT 2 cut(s) 386, 1697
Bme1390I CCNGG 2 cut(s) 1351, 1534
BmiI GGNNCC 6 cut(s) 198, 483, 1155, 1240, 1513, 1679
BmrFI CCNGG 2 cut(s) 1351, 1534
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 5 cut(s) 208, 1038, 1198, 1249, 1576
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 1 cut(s) 762
BpmI CTGGAG 2 cut(s) 392, 1516
BsaAI YACGTR 2 cut(s) 242, 682
BsaI GGTCTC 1 cut(s) 1283
BsaJI CCNNGG 6 cut(s) 631, 1080, 1157, 1349, 1466, 1604
BsaWI WCCGGW 1 cut(s) 1508
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 370, 1110, 1533, 1605
Bse118I RCCGGY 1 cut(s) 1508
Bse1I ACTGG 4 cut(s) 78, 121, 1393, 1610
Bse3DI GCAATG 2 cut(s) 138, 645
BseBI CCWGG 2 cut(s) 1351, 1534
BseDI CCNNGG 6 cut(s) 631, 1080, 1157, 1349, 1466, 1604
BseGI GGATG 2 cut(s) 1120, 1542
BseLI CCNNNNNNNGG 5 cut(s) 127, 370, 1110, 1533, 1605
BseMI GCAATG 2 cut(s) 138, 645
BseNI ACTGG 4 cut(s) 78, 121, 1393, 1610
BseRI GAGGAG 1 cut(s) 63
BseSI GKGCMC 1 cut(s) 1687
BseXI GCAGC 1 cut(s) 1157
BsgI GTGCAG 1 cut(s) 737
Bsh1236I CGCG 2 cut(s) 1026, 1545
BshFI GGCC 3 cut(s) 1085, 1103, 1609
BshNI GGYRCC 2 cut(s) 1511, 1677
BshTI ACCGGT 1 cut(s) 1508
BsiHKAI GWGCWC 1 cut(s) 1687
BsiSI CCGG 2 cut(s) 1509, 1640
BslFI GGGAC 2 cut(s) 92, 366
BslI CCNNNNNNNGG 5 cut(s) 127, 370, 1110, 1533, 1605
BsmAI GTCTC 2 cut(s) 968, 1283
BsmFI GGGAC 2 cut(s) 92, 366
BsmI GAATGC 3 cut(s) 296, 1398, 1589
BsnI GGCC 3 cut(s) 1085, 1103, 1609
Bso31I GGTCTC 1 cut(s) 1283
Bsp1286I GDGCHC 1 cut(s) 1687
Bsp1407I TGTACA 1 cut(s) 1664
Bsp143I GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
Bsp19I CCATGG 1 cut(s) 1466
Bsp68I TCGCGA 1 cut(s) 1026
BspACI CCGC 3 cut(s) 84, 469, 1630
BspANI GGCC 3 cut(s) 1085, 1103, 1609
BspFNI CGCG 2 cut(s) 1026, 1545
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 6 cut(s) 198, 483, 1155, 1240, 1513, 1679
BspMAI CTGCAG 1 cut(s) 1147
BspPI GGATC 6 cut(s) 476, 489, 1148, 1161, 1264, 1621
BspT107I GGYRCC 2 cut(s) 1511, 1677
BspTNI GGTCTC 1 cut(s) 1283
BsrBI CCGCTC 1 cut(s) 471
BsrDI GCAATG 2 cut(s) 138, 645
BsrFI RCCGGY 1 cut(s) 1508
BsrGI TGTACA 1 cut(s) 1664
BsrI ACTGG 4 cut(s) 78, 121, 1393, 1610
BssAI RCCGGY 1 cut(s) 1508
BssECI CCNNGG 6 cut(s) 631, 1080, 1157, 1349, 1466, 1604
BssMI GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
BssT1I CCWWGG 3 cut(s) 631, 1466, 1604
Bst2UI CCWGG 2 cut(s) 1351, 1534
Bst4CI ACNGT 3 cut(s) 398, 1254, 1682
Bst6I CTCTTC 1 cut(s) 18
BstAPI GCANNNNNTGC 1 cut(s) 1142
BstAUI TGTACA 1 cut(s) 1664
BstBAI YACGTR 2 cut(s) 242, 682
BstC8I GCNNGC 2 cut(s) 825, 1543
BstDEI CTNAG 2 cut(s) 204, 1645
BstDSI CCRYGG 1 cut(s) 1466
BstF5I GGATG 2 cut(s) 1120, 1542
BstFNI CGCG 2 cut(s) 1026, 1545
BstKTI GATC 6 cut(s) 67, 484, 817, 1156, 1272, 1629
BstMAI GTCTC 2 cut(s) 968, 1283
BstMBI GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
BstMWI GCNNNNNNNGC 1 cut(s) 1142
BstNI CCWGG 2 cut(s) 1351, 1534
BstSCI CCNGG 2 cut(s) 1349, 1532
BstSFI CTRYAG 2 cut(s) 818, 1143
BstSLI GKGCMC 1 cut(s) 1687
BstUI CGCG 2 cut(s) 1026, 1545
BstV1I GCAGC 1 cut(s) 1157
BstV2I GAAGAC 1 cut(s) 762
BstX2I RGATCY 2 cut(s) 481, 1153
BstYI RGATCY 2 cut(s) 481, 1153
BsuI GTATCC 1 cut(s) 1530
BsuRI GGCC 3 cut(s) 1085, 1103, 1609
BtgI CCRYGG 1 cut(s) 1466
BtgZI GCGATG 1 cut(s) 111
BtsCI GGATG 2 cut(s) 1120, 1542
BtsI GCAGTG 1 cut(s) 607
BtsIMutI CAGTG 3 cut(s) 607, 1400, 1685
BtuMI TCGCGA 1 cut(s) 1026
Cac8I GCNNGC 2 cut(s) 825, 1543
CciI TCATGA 1 cut(s) 1015
Cfr10I RCCGGY 1 cut(s) 1508
CseI GACGC 1 cut(s) 259
Csp6I GTAC 5 cut(s) 385, 458, 1665, 1678, 1696
CspAI ACCGGT 1 cut(s) 1508
CviAII CATG 5 cut(s) 888, 1016, 1087, 1467, 1624
CviQI GTAC 5 cut(s) 385, 458, 1665, 1678, 1696
DdeI CTNAG 2 cut(s) 204, 1645
DpnI GATC 6 cut(s) 66, 483, 816, 1155, 1271, 1628
DpnII GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
DrdI GACNNNNNNGTC 1 cut(s) 1051
DseDI GACNNNNNNGTC 1 cut(s) 1051
EaeI YGGCCR 1 cut(s) 1101
Eam1104I CTCTTC 1 cut(s) 18
EarI CTCTTC 1 cut(s) 18
Eco130I CCWWGG 3 cut(s) 631, 1466, 1604
Eco31I GGTCTC 1 cut(s) 1283
Eco32I GATATC 1 cut(s) 745
Eco57I CTGAAG 2 cut(s) 507, 851
Eco72I CACGTG 1 cut(s) 682
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 1349, 1532
EcoRV GATATC 1 cut(s) 745
EcoT14I CCWWGG 3 cut(s) 631, 1466, 1604
EcoT22I ATGCAT 3 cut(s) 1264, 1591, 1657
ErhI CCWWGG 3 cut(s) 631, 1466, 1604
FaeI CATG 5 cut(s) 891, 1019, 1090, 1470, 1627
FalI AAGNNNNNCTT 4 cut(s) 1277, 1309, 1628, 1660
FaqI GGGAC 2 cut(s) 92, 366
FatI CATG 5 cut(s) 887, 1015, 1086, 1466, 1623
FauNDI CATATG 2 cut(s) 1264, 1582
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 1 cut(s) 1146
FokI GGATG 2 cut(s) 1127, 1549
Fsp4HI GCNGC 1 cut(s) 1146
FspBI CTAG 1 cut(s) 588
GluI GCNGC 1 cut(s) 1146
GsuI CTGGAG 2 cut(s) 392, 1516
HaeIII GGCC 3 cut(s) 1085, 1103, 1609
HapII CCGG 2 cut(s) 1509, 1640
HgaI GACGC 1 cut(s) 259
Hin1II CATG 5 cut(s) 891, 1019, 1090, 1470, 1627
HinfI GANTC 7 cut(s) 26, 799, 1304, 1319, 1325, 1337, 1471
HpaII CCGG 2 cut(s) 1509, 1640
HphI GGTGA 5 cut(s) 119, 352, 707, 1001, 1217
Hpy166II GTNNAC 3 cut(s) 1665, 1667, 1685
Hpy188III TCNNGA 9 cut(s) 68, 371, 485, 518, 899, 1016, 1025, 1069, 1280
Hpy8I GTNNAC 3 cut(s) 1665, 1667, 1685
Hpy99I CGWCG 1 cut(s) 675
HpyAV CCTTC 5 cut(s) 127, 425, 914, 1022, 1325
HpyCH4III ACNGT 3 cut(s) 398, 1254, 1682
HpyCH4IV ACGT 2 cut(s) 241, 681
HpyF10VI GCNNNNNNNGC 1 cut(s) 1142
HpyF3I CTNAG 2 cut(s) 204, 1645
HpySE526I ACGT 2 cut(s) 241, 681
Hsp92II CATG 5 cut(s) 891, 1019, 1090, 1470, 1627
KpnI GGTACC 1 cut(s) 1681
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
LmnI GCTCC 2 cut(s) 942, 1238
Lsp1109I GCAGC 1 cut(s) 1157
LweI GCATC 5 cut(s) 208, 1038, 1198, 1249, 1576
MaeI CTAG 1 cut(s) 588
MaeII ACGT 2 cut(s) 241, 681
MaeIII GTNAC 1 cut(s) 89
MalI GATC 6 cut(s) 66, 483, 816, 1155, 1271, 1628
MbiI CCGCTC 1 cut(s) 471
MboI GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
MflI RGATCY 2 cut(s) 481, 1153
MhlI GDGCHC 1 cut(s) 1687
MlsI TGGCCA 1 cut(s) 1103
MluNI TGGCCA 1 cut(s) 1103
MlyI GAGTC 1 cut(s) 1480
MmeI TCCRAC 3 cut(s) 105, 1071, 1416
Mox20I TGGCCA 1 cut(s) 1103
Mph1103I ATGCAT 3 cut(s) 1264, 1591, 1657
MscI TGGCCA 1 cut(s) 1103
MseI TTAA 7 cut(s) 249, 261, 327, 558, 738, 1164, 1200
MslI CAYNNNNRTG 2 cut(s) 1014, 1660
Msp20I TGGCCA 1 cut(s) 1103
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 2 cut(s) 1509, 1640
MspR9I CCNGG 2 cut(s) 1351, 1534
Mva1269I GAATGC 3 cut(s) 296, 1398, 1589
MvaI CCWGG 2 cut(s) 1351, 1534
MvnI CGCG 2 cut(s) 1026, 1545
MwoI GCNNNNNNNGC 1 cut(s) 1142
NcoI CCATGG 1 cut(s) 1466
NdeI CATATG 2 cut(s) 1264, 1582
NdeII GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
NlaIII CATG 5 cut(s) 891, 1019, 1090, 1470, 1627
NlaIV GGNNCC 6 cut(s) 198, 483, 1155, 1240, 1513, 1679
NmeAIII GCCGAG 1 cut(s) 1105
NmuCI GTSAC 1 cut(s) 89
NruI TCGCGA 1 cut(s) 1026
NsiI ATGCAT 3 cut(s) 1264, 1591, 1657
PagI TCATGA 1 cut(s) 1015
PctI GAATGC 3 cut(s) 296, 1398, 1589
PfeI GAWTC 6 cut(s) 26, 799, 1304, 1319, 1325, 1337
PflMI CCANNNNNTGG 1 cut(s) 1110
PfoI TCCNGGA 1 cut(s) 1532
PinAI ACCGGT 1 cut(s) 1508
PkrI GCNGC 1 cut(s) 1147
PleI GAGTC 1 cut(s) 1479
PmaCI CACGTG 1 cut(s) 682
PmlI CACGTG 1 cut(s) 682
PpsI GAGTC 1 cut(s) 1479
Ppu21I YACGTR 2 cut(s) 242, 682
Psp6I CCWGG 2 cut(s) 1349, 1532
PspCI CACGTG 1 cut(s) 682
PspGI CCWGG 2 cut(s) 1349, 1532
PspN4I GGNNCC 6 cut(s) 198, 483, 1155, 1240, 1513, 1679
PsrI GAACNNNNNNTAC 2 cut(s) 1446, 1478
PstI CTGCAG 1 cut(s) 1147
PsuI RGATCY 2 cut(s) 481, 1153
RruI TCGCGA 1 cut(s) 1026
RsaI GTAC 5 cut(s) 386, 459, 1666, 1679, 1697
RsaNI GTAC 5 cut(s) 385, 458, 1665, 1678, 1696
RseI CAYNNNNRTG 2 cut(s) 1014, 1660
SaqAI TTAA 7 cut(s) 249, 261, 327, 558, 738, 1164, 1200
SatI GCNGC 1 cut(s) 1146
Sau3AI GATC 6 cut(s) 64, 481, 814, 1153, 1269, 1626
ScaI AGTACT 2 cut(s) 386, 1697
SchI GAGTC 1 cut(s) 1480
ScrFI CCNGG 2 cut(s) 1351, 1534
SduI GDGCHC 1 cut(s) 1687
SfaNI GCATC 5 cut(s) 208, 1038, 1198, 1249, 1576
SfcI CTRYAG 2 cut(s) 818, 1143
SgrAI CRCCGGYG 1 cut(s) 1508
SmiMI CAYNNNNRTG 2 cut(s) 1014, 1660
SpeI ACTAGT 1 cut(s) 587
SsiI CCGC 3 cut(s) 84, 469, 1630
SspMI CTAG 1 cut(s) 588
StyD4I CCNGG 2 cut(s) 1349, 1532
StyI CCWWGG 3 cut(s) 631, 1466, 1604
TaaI ACNGT 3 cut(s) 398, 1254, 1682
TaiI ACGT 2 cut(s) 244, 684
TaqI TCGA 4 cut(s) 517, 750, 760, 1406
TatI WGTACW 3 cut(s) 384, 1664, 1695
TfiI GAWTC 6 cut(s) 26, 799, 1304, 1319, 1325, 1337
Tru1I TTAA 7 cut(s) 249, 261, 327, 558, 738, 1164, 1200
Tru9I TTAA 7 cut(s) 249, 261, 327, 558, 738, 1164, 1200
TscAI CASTG 3 cut(s) 607, 1400, 1692
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 1 cut(s) 1145
Tsp45I GTSAC 1 cut(s) 89
TspGWI ACGGA 1 cut(s) 58
TspRI CASTG 3 cut(s) 607, 1400, 1692
Van91I CCANNNNNTGG 1 cut(s) 1110
VneI GTGCAC 1 cut(s) 1683
XapI RAATTY 5 cut(s) 110, 176, 332, 343, 1181
XspI CTAG 1 cut(s) 588
ZrmI AGTACT 2 cut(s) 386, 1697
Zsp2I ATGCAT 3 cut(s) 1264, 1591, 1657
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.