MD09G1162900.v1.1

23 kDa jasmonate-induced protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001765
Physical Location & Seq
Reverse (-)
13265353 .. 13266286
934 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1162900.v1.1.491

Sequence Viewer

Length: 636 bp
ATGACGTATAACGTTTTTGGCAACCCCATCACAGATGAAACTTTGAAGGGGATGCCAGAGTATGCCGACAAGCAAATAGAAAGATTAGACAGGGCACACGTGGCGTTGAATATGAAAAATGCCGTGGAGAAGGACTTCAACGCTAGAACATATGTGGAGAAACTGAAGGAACAGTATGGCGACGGAGTCTCCACACTCTGCCTTGTTTACAATGCAACTGGGGACACCATAAGCTATGCTAATAAGAAAGATTGGTATGGGCACATCGGGAACGCACCATACCCACCACTGATTGCAAATGGTCAGTGGGGTGCGTTTCTGCACGTCAAAACTGCAGGAGCGTCTTCTGGATCAACGGCTGCTGTTGTGTACCAAGGACTAAATCATGTTAGCAACGAATGCGACTGGATGTTGTCTTGGAGCAACCCATGGGACAGGACTAGCTCGGACAACAAGGTCTATACAGATATTCAATTCGCTGATAACTTTGAAACTCAATGGATCTTAATCTATGGTTTCTTGGCGAGAGGTCAAGCGTACATGAAGAAGACATGGCATGGATGCGTGTCAATCATGTCAACTGGCAGTGACAGTTCCCCAATTGTTGAAGCAATACTCACTTTAGAAAATGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

212

Amino Acids

23.54

Weight (kDa)

5.31

Isoelectric Point (pI)

27.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nakanori PF21230 32 - 195 1.2e-46 Nakanori-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 455
AclI AACGTT 1 cut(s) 12
AclWI GGATC 2 cut(s) 358, 509
AcuI CTGAAG 1 cut(s) 185
AcvI CACGTG 1 cut(s) 100
AfaI GTAC 2 cut(s) 371, 539
AflIII ACRYGT 1 cut(s) 97
AgsI TTSAA 6 cut(s) 46, 109, 139, 473, 491, 608
AjiI CACGTC 1 cut(s) 325
AluBI AGCT 2 cut(s) 234, 444
AluI AGCT 2 cut(s) 234, 444
Alw26I GTCTC 1 cut(s) 193
AlwI GGATC 2 cut(s) 358, 509
ApeKI GCWGC 1 cut(s) 359
Asp700I GAANNNNTTC 1 cut(s) 134
BaeGI GKGCMC 2 cut(s) 97, 264
BbrPI CACGTG 1 cut(s) 100
BbsI GAAGAC 2 cut(s) 336, 554
BbvI GCAGC 1 cut(s) 346
BccI CCATC 1 cut(s) 35
BceAI ACGGC 2 cut(s) 107, 372
BcoDI GTCTC 1 cut(s) 193
BfaI CTAG 2 cut(s) 144, 441
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 1 cut(s) 360
BlsI GCNGC 1 cut(s) 361
BmgBI CACGTC 1 cut(s) 325
BmrI ACTGGG 1 cut(s) 228
BmsI GCATC 2 cut(s) 42, 551
BmuI ACTGGG 1 cut(s) 228
BpiI GAAGAC 2 cut(s) 336, 554
BsaAI YACGTR 1 cut(s) 100
BsaBI GATNNNNATC 1 cut(s) 506
BsaJI CCNNGG 3 cut(s) 123, 373, 428
BsaXI ACNNNNNCTCC 2 cut(s) 173, 203
Bse1I ACTGG 3 cut(s) 223, 410, 586
Bse8I GATNNNNATC 1 cut(s) 506
BseDI CCNNGG 3 cut(s) 123, 373, 428
BseGI GGATG 3 cut(s) 57, 414, 566
BseJI GATNNNNATC 1 cut(s) 506
BseNI ACTGG 3 cut(s) 223, 410, 586
BseSI GKGCMC 2 cut(s) 97, 264
BseXI GCAGC 1 cut(s) 346
BsgI GTGCAG 1 cut(s) 305
BslFI GGGAC 2 cut(s) 236, 446
BsmAI GTCTC 1 cut(s) 193
BsmFI GGGAC 2 cut(s) 236, 446
BsmI GAATGC 1 cut(s) 404
Bsp1286I GDGCHC 2 cut(s) 97, 264
Bsp143I GATC 2 cut(s) 350, 501
Bsp19I CCATGG 1 cut(s) 428
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 358, 509
BsrI ACTGG 3 cut(s) 223, 410, 586
BssECI CCNNGG 3 cut(s) 123, 373, 428
BssMI GATC 2 cut(s) 350, 501
BssT1I CCWWGG 2 cut(s) 373, 428
Bst4CI ACNGT 2 cut(s) 174, 593
BstAPI GCANNNNNTGC 1 cut(s) 399
BstBAI YACGTR 1 cut(s) 100
BstDSI CCRYGG 2 cut(s) 123, 428
BstF5I GGATG 3 cut(s) 57, 414, 566
BstKTI GATC 2 cut(s) 353, 504
BstMAI GTCTC 1 cut(s) 193
BstMBI GATC 2 cut(s) 350, 501
BstMWI GCNNNNNNNGC 2 cut(s) 101, 399
BstSFI CTRYAG 1 cut(s) 333
BstSLI GKGCMC 2 cut(s) 97, 264
BstV1I GCAGC 1 cut(s) 346
BstV2I GAAGAC 2 cut(s) 336, 554
BstX2I RGATCY 1 cut(s) 501
BstYI RGATCY 1 cut(s) 501
BtgI CCRYGG 2 cut(s) 123, 428
BtrI CACGTC 1 cut(s) 325
BtsCI GGATG 3 cut(s) 57, 414, 566
BtsI GCAGTG 1 cut(s) 592
BtsIMutI CAGTG 3 cut(s) 287, 311, 592
CseI GACGC 1 cut(s) 330
Csp6I GTAC 2 cut(s) 370, 538
CviAII CATG 6 cut(s) 386, 429, 541, 552, 557, 574
CviJI RGCY 3 cut(s) 234, 359, 444
CviKI_1 RGCY 3 cut(s) 234, 359, 444
CviQI GTAC 2 cut(s) 370, 538
DpnI GATC 2 cut(s) 352, 503
DpnII GATC 2 cut(s) 350, 501
DrdI GACNNNNNNGTC 1 cut(s) 455
DseDI GACNNNNNNGTC 1 cut(s) 455
Eco130I CCWWGG 2 cut(s) 373, 428
Eco57I CTGAAG 1 cut(s) 185
Eco72I CACGTG 1 cut(s) 100
EcoT14I CCWWGG 2 cut(s) 373, 428
ErhI CCWWGG 2 cut(s) 373, 428
FaeI CATG 6 cut(s) 389, 432, 544, 555, 560, 577
FaqI GGGAC 2 cut(s) 236, 446
FatI CATG 6 cut(s) 385, 428, 540, 551, 556, 573
FauNDI CATATG 1 cut(s) 151
Fnu4HI GCNGC 1 cut(s) 360
FokI GGATG 3 cut(s) 64, 421, 573
Fsp4HI GCNGC 1 cut(s) 360
FspBI CTAG 2 cut(s) 144, 441
GluI GCNGC 1 cut(s) 360
HgaI GACGC 1 cut(s) 330
Hin1II CATG 6 cut(s) 389, 432, 544, 555, 560, 577
HincII GTYRAC 1 cut(s) 579
HindII GTYRAC 1 cut(s) 579
HinfI GANTC 1 cut(s) 186
Hpy166II GTNNAC 3 cut(s) 208, 370, 579
Hpy188I TCNGA 1 cut(s) 448
Hpy188III TCNNGA 2 cut(s) 268, 348
Hpy8I GTNNAC 3 cut(s) 208, 370, 579
Hpy99I CGWCG 1 cut(s) 185
HpyAV CCTTC 3 cut(s) 40, 124, 160
HpyCH4III ACNGT 2 cut(s) 174, 593
HpyCH4IV ACGT 4 cut(s) 5, 12, 99, 324
HpyCH4V TGCA 4 cut(s) 215, 296, 322, 335
HpyF10VI GCNNNNNNNGC 2 cut(s) 101, 399
HpySE526I ACGT 4 cut(s) 5, 12, 99, 324
Hsp92II CATG 6 cut(s) 389, 432, 544, 555, 560, 577
Kzo9I GATC 2 cut(s) 350, 501
LmnI GCTCC 2 cut(s) 338, 420
LpnPI CCDG 8 cut(s) 69, 76, 204, 321, 333, 391, 421, 567
Lsp1109I GCAGC 1 cut(s) 346
LweI GCATC 2 cut(s) 42, 551
MaeI CTAG 2 cut(s) 144, 441
MaeII ACGT 4 cut(s) 5, 12, 99, 324
MaeIII GTNAC 1 cut(s) 587
MalI GATC 2 cut(s) 352, 503
MboI GATC 2 cut(s) 350, 501
MboII GAAGA 3 cut(s) 336, 556, 559
MfeI CAATTG 1 cut(s) 600
MflI RGATCY 1 cut(s) 501
MhlI GDGCHC 2 cut(s) 97, 264
MluCI AATT 2 cut(s) 473, 600
MlyI GAGTC 1 cut(s) 195
MnlI CCTC 1 cut(s) 521
MroXI GAANNNNTTC 1 cut(s) 134
MseI TTAA 1 cut(s) 506
MunI CAATTG 1 cut(s) 600
Mva1269I GAATGC 1 cut(s) 404
MwoI GCNNNNNNNGC 2 cut(s) 101, 399
NcoI CCATGG 1 cut(s) 428
NdeI CATATG 1 cut(s) 151
NdeII GATC 2 cut(s) 350, 501
NlaIII CATG 6 cut(s) 389, 432, 544, 555, 560, 577
NmuCI GTSAC 1 cut(s) 587
PctI GAATGC 1 cut(s) 404
PdmI GAANNNNTTC 1 cut(s) 134
PflFI GACNNNGTC 1 cut(s) 185
PkrI GCNGC 1 cut(s) 361
PleI GAGTC 1 cut(s) 194
PmaCI CACGTG 1 cut(s) 100
PmlI CACGTG 1 cut(s) 100
PpsI GAGTC 1 cut(s) 194
Ppu21I YACGTR 1 cut(s) 100
Psp1406I AACGTT 1 cut(s) 12
PspCI CACGTG 1 cut(s) 100
PsrI GAACNNNNNNTAC 2 cut(s) 263, 295
PstI CTGCAG 1 cut(s) 337
PsuI RGATCY 1 cut(s) 501
PsyI GACNNNGTC 1 cut(s) 185
RsaI GTAC 2 cut(s) 371, 539
RsaNI GTAC 2 cut(s) 370, 538
SaqAI TTAA 1 cut(s) 506
SatI GCNGC 1 cut(s) 360
Sau3AI GATC 2 cut(s) 350, 501
SchI GAGTC 1 cut(s) 195
SduI GDGCHC 2 cut(s) 97, 264
SetI ASST 8 cut(s) 8, 15, 102, 236, 327, 446, 459, 532
SfaNI GCATC 2 cut(s) 42, 551
SfcI CTRYAG 1 cut(s) 333
Sse9I AATT 2 cut(s) 473, 600
SspMI CTAG 2 cut(s) 144, 441
StyI CCWWGG 2 cut(s) 373, 428
TaaI ACNGT 2 cut(s) 174, 593
TaiI ACGT 4 cut(s) 8, 15, 102, 327
TasI AATT 2 cut(s) 473, 600
Tru1I TTAA 1 cut(s) 506
Tru9I TTAA 1 cut(s) 506
TscAI CASTG 3 cut(s) 294, 311, 592
TseFI GTSAC 1 cut(s) 587
TseI GCWGC 1 cut(s) 359
Tsp45I GTSAC 1 cut(s) 587
TspDTI ATGAA 3 cut(s) 51, 128, 557
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 3 cut(s) 294, 311, 592
Tth111I GACNNNGTC 1 cut(s) 185
XmnI GAANNNNTTC 1 cut(s) 134
XspI CTAG 2 cut(s) 144, 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.