Rroxscaffold_2G00101280

23 kDa jasmonate-induced protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001765
Physical Location & Seq
Forward (+)
23263943 .. 23280242
16300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00101280.1

Sequence Viewer

Length: 642 bp
ATGGCGAACGTGTTTGGCAACCCCATAACCGACTCAACTTTAGCGGCAATGCCAGAGTATGCCAACAAGCAACTAACAGCAGTTGATAGGGCACGCGTGGCTCTCAACCTGCAAAGCGTCGACGGGAAGGCTACCGACGCACTCACCTACGTGGAGAATTTGAAGAAGCAGTACGGAGGGGAAGTACCCGCCACACTTTGCCTCCTTTACAATGCTACTGGGAACACTCTAACATTTGTTACTGCTATAGATGAGTATGGGCACATTGGACCAGCCCCATACCCAATGCGAATTCAAAATGGCCAGTGGGGTGCATTTCTGCACGTCAAAACCGCTGAAACGGAAACCGGATCTTATGCCGGTGTTGTGTATCGCGGCAAGAACCCCGATGGCGTCGATTGGGACTGCATGATGGCATGGCGTAACCCGTTTGTGAATACCAACAACTCGGTCTGGTCTGAGATCCGCGAAATGAACCACTTCGATAGTGGAGCATGGAGTACAATAGCTAATCGTTTGTTACAGCTTCGCTACAACCAGAGTGACACATGGCAGGGAGGCTTGTCAACTATAGCAACTGGCAGGGACACTTCGCCTATAGTGGAAGCACTGTTCACTTTAGAAGAGGCCACCGAAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

213

Amino Acids

23.42

Weight (kDa)

4.82

Isoelectric Point (pI)

31.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nakanori PF21230 31 - 194 1.2e-33 Nakanori-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 117
AccI GTMKAC 1 cut(s) 120
AccII CGCG 3 cut(s) 96, 375, 468
AciI CCGC 5 cut(s) 44, 189, 333, 375, 466
AclWI GGATC 2 cut(s) 358, 457
AcoI YGGCCR 1 cut(s) 301
AcsI RAATTY 2 cut(s) 157, 291
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 3 cut(s) 173, 186, 502
AflIII ACRYGT 2 cut(s) 9, 94
AgsI TTSAA 2 cut(s) 163, 296
AjiI CACGTC 1 cut(s) 325
AleI CACNNNNGTG 1 cut(s) 149
AloI GAACNNNNNNTCC 2 cut(s) 596, 628
AluBI AGCT 2 cut(s) 509, 526
AluI AGCT 2 cut(s) 509, 526
AlwI GGATC 2 cut(s) 358, 457
AoxI GGCC 2 cut(s) 301, 627
ApoI RAATTY 2 cut(s) 157, 291
Asp700I GAANNNNTTC 1 cut(s) 479
AspS9I GGNCC 1 cut(s) 269
AsuHPI GGTGA 1 cut(s) 136
AvaII GGWCC 1 cut(s) 269
BaeGI GKGCMC 2 cut(s) 94, 264
BalI TGGCCA 1 cut(s) 303
BarI GAAGNNNNNNTAC 2 cut(s) 155, 187
BccI CCATC 2 cut(s) 383, 406
BfmI CTRYAG 3 cut(s) 246, 570, 597
BfuAI ACCTGC 1 cut(s) 117
BisI GCNGC 2 cut(s) 45, 376
BlsI GCNGC 2 cut(s) 46, 377
Bme18I GGWCC 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 325
BmgT120I GGNCC 1 cut(s) 269
BmrI ACTGGG 1 cut(s) 228
BmuI ACTGGG 1 cut(s) 228
BsaAI YACGTR 1 cut(s) 151
BsaHI GRCGYC 1 cut(s) 393
BsaWI WCCGGW 1 cut(s) 347
BsaXI ACNNNNNCTCC 4 cut(s) 168, 186, 198, 216
Bse118I RCCGGY 1 cut(s) 359
Bse1I ACTGG 3 cut(s) 223, 304, 583
Bse3DI GCAATG 1 cut(s) 54
BseMI GCAATG 1 cut(s) 54
BseMII CTCAG 1 cut(s) 450
BseNI ACTGG 3 cut(s) 223, 304, 583
BseSI GKGCMC 2 cut(s) 94, 264
BsgI GTGCAG 1 cut(s) 305
Bsh1236I CGCG 3 cut(s) 96, 375, 468
BshFI GGCC 2 cut(s) 303, 629
BsiSI CCGG 2 cut(s) 348, 360
BslFI GGGAC 2 cut(s) 416, 599
BsmFI GGGAC 2 cut(s) 416, 599
BsnI GGCC 2 cut(s) 303, 629
Bsp1286I GDGCHC 2 cut(s) 94, 264
Bsp143I GATC 2 cut(s) 350, 462
BspACI CCGC 5 cut(s) 44, 189, 333, 375, 466
BspANI GGCC 2 cut(s) 303, 629
BspCNI CTCAG 1 cut(s) 451
BspFNI CGCG 3 cut(s) 96, 375, 468
BspMI ACCTGC 1 cut(s) 117
BspPI GGATC 2 cut(s) 358, 457
BsrDI GCAATG 1 cut(s) 54
BsrFI RCCGGY 1 cut(s) 359
BsrI ACTGG 3 cut(s) 223, 304, 583
BssAI RCCGGY 1 cut(s) 359
BssMI GATC 2 cut(s) 350, 462
BssNI GRCGYC 1 cut(s) 393
Bst4CI ACNGT 1 cut(s) 612
Bst6I CTCTTC 1 cut(s) 618
BstACI GRCGYC 1 cut(s) 393
BstBAI YACGTR 1 cut(s) 151
BstC8I GCNNGC 1 cut(s) 94
BstDEI CTNAG 1 cut(s) 459
BstFNI CGCG 3 cut(s) 96, 375, 468
BstKTI GATC 2 cut(s) 353, 465
BstMBI GATC 2 cut(s) 350, 462
BstMWI GCNNNNNNNGC 3 cut(s) 98, 137, 635
BstSFI CTRYAG 3 cut(s) 246, 570, 597
BstSLI GKGCMC 2 cut(s) 94, 264
BstUI CGCG 3 cut(s) 96, 375, 468
BstX2I RGATCY 2 cut(s) 350, 462
BstYI RGATCY 2 cut(s) 350, 462
BsuRI GGCC 2 cut(s) 303, 629
BtrI CACGTC 1 cut(s) 325
BtsIMutI CAGTG 2 cut(s) 311, 608
BveI ACCTGC 1 cut(s) 117
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 359
Cfr13I GGNCC 1 cut(s) 269
CseI GACGC 3 cut(s) 106, 146, 382
Csp6I GTAC 3 cut(s) 172, 185, 501
CviAII CATG 4 cut(s) 409, 417, 495, 549
CviJI RGCY 9 cut(s) 101, 131, 275, 303, 509, 526, 561, 629, 638
CviKI_1 RGCY 9 cut(s) 101, 131, 275, 303, 509, 526, 561, 629, 638
CviQI GTAC 3 cut(s) 172, 185, 501
DdeI CTNAG 1 cut(s) 459
DpnI GATC 2 cut(s) 352, 464
DpnII GATC 2 cut(s) 350, 462
EaeI YGGCCR 1 cut(s) 301
Eam1104I CTCTTC 1 cut(s) 618
EarI CTCTTC 1 cut(s) 618
Eco47I GGWCC 1 cut(s) 269
EcoRI GAATTC 1 cut(s) 291
FaeI CATG 4 cut(s) 412, 420, 498, 552
FaqI GGGAC 2 cut(s) 416, 599
FatI CATG 4 cut(s) 408, 416, 494, 548
FauI CCCGC 1 cut(s) 196
FblI GTMKAC 1 cut(s) 120
Fnu4HI GCNGC 2 cut(s) 45, 376
Fsp4HI GCNGC 2 cut(s) 45, 376
GluI GCNGC 2 cut(s) 45, 376
HaeIII GGCC 2 cut(s) 303, 629
HapII CCGG 2 cut(s) 348, 360
HgaI GACGC 3 cut(s) 106, 146, 382
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 4 cut(s) 412, 420, 498, 552
HincII GTYRAC 2 cut(s) 121, 567
HindII GTYRAC 2 cut(s) 121, 567
HinfI GANTC 1 cut(s) 32
HpaII CCGG 2 cut(s) 348, 360
HphI GGTGA 1 cut(s) 136
Hpy166II GTNNAC 3 cut(s) 121, 567, 615
Hpy188I TCNGA 1 cut(s) 460
Hpy8I GTNNAC 3 cut(s) 121, 567, 615
Hpy99I CGWCG 4 cut(s) 122, 125, 140, 398
HpyAV CCTTC 1 cut(s) 121
HpyCH4III ACNGT 1 cut(s) 612
HpyCH4IV ACGT 3 cut(s) 9, 150, 324
HpyCH4V TGCA 4 cut(s) 112, 314, 322, 408
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 137, 635
HpyF3I CTNAG 1 cut(s) 459
HpySE526I ACGT 3 cut(s) 9, 150, 324
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 4 cut(s) 412, 420, 498, 552
Kzo9I GATC 2 cut(s) 350, 462
LmnI GCTCC 1 cut(s) 491
MaeII ACGT 3 cut(s) 9, 150, 324
MaeIII GTNAC 4 cut(s) 238, 422, 519, 542
MalI GATC 2 cut(s) 352, 464
MboI GATC 2 cut(s) 350, 462
MboII GAAGA 2 cut(s) 175, 635
MflI RGATCY 2 cut(s) 350, 462
MhlI GDGCHC 2 cut(s) 94, 264
MlsI TGGCCA 1 cut(s) 303
MluCI AATT 2 cut(s) 157, 291
MluI ACGCGT 1 cut(s) 94
MluNI TGGCCA 1 cut(s) 303
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 4 cut(s) 170, 212, 551, 619
Mox20I TGGCCA 1 cut(s) 303
MroXI GAANNNNTTC 1 cut(s) 479
MscI TGGCCA 1 cut(s) 303
MslI CAYNNNNRTG 1 cut(s) 149
Msp20I TGGCCA 1 cut(s) 303
MspA1I CMGCKG 1 cut(s) 335
MspI CCGG 2 cut(s) 348, 360
MvnI CGCG 3 cut(s) 96, 375, 468
MwoI GCNNNNNNNGC 3 cut(s) 98, 137, 635
NdeII GATC 2 cut(s) 350, 462
NlaIII CATG 4 cut(s) 412, 420, 498, 552
NmuCI GTSAC 1 cut(s) 542
OliI CACNNNNGTG 1 cut(s) 149
PdmI GAANNNNTTC 1 cut(s) 479
PkrI GCNGC 2 cut(s) 46, 377
PleI GAGTC 1 cut(s) 26
PpsI GAGTC 1 cut(s) 26
Ppu21I YACGTR 1 cut(s) 151
PspPI GGNCC 1 cut(s) 269
PsuI RGATCY 2 cut(s) 350, 462
RsaI GTAC 3 cut(s) 173, 186, 502
RsaNI GTAC 3 cut(s) 172, 185, 501
RseI CAYNNNNRTG 1 cut(s) 149
SalI GTCGAC 1 cut(s) 119
SatI GCNGC 2 cut(s) 45, 376
Sau3AI GATC 2 cut(s) 350, 462
Sau96I GGNCC 1 cut(s) 269
SchI GAGTC 1 cut(s) 26
SduI GDGCHC 2 cut(s) 94, 264
SetI ASST 7 cut(s) 12, 111, 149, 153, 327, 511, 528
SfcI CTRYAG 3 cut(s) 246, 570, 597
SgrDI CGTCGACG 1 cut(s) 119
SinI GGWCC 1 cut(s) 269
SmiMI CAYNNNNRTG 1 cut(s) 149
Sse9I AATT 2 cut(s) 157, 291
SsiI CCGC 5 cut(s) 44, 189, 333, 375, 466
TaaI ACNGT 1 cut(s) 612
TaiI ACGT 3 cut(s) 12, 153, 327
TaqI TCGA 3 cut(s) 120, 396, 483
TaqII GACCGA 1 cut(s) 439
TasI AATT 2 cut(s) 157, 291
TatI WGTACW 1 cut(s) 500
TauI GCSGC 2 cut(s) 47, 378
TscAI CASTG 2 cut(s) 311, 615
TseFI GTSAC 1 cut(s) 542
Tsp45I GTSAC 1 cut(s) 542
TspDTI ATGAA 1 cut(s) 488
TspGWI ACGGA 2 cut(s) 189, 356
TspRI CASTG 2 cut(s) 311, 615
VpaK11BI GGWCC 1 cut(s) 269
XapI RAATTY 2 cut(s) 157, 291
XcmI CCANNNNNNNNNTGG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 120
XmnI GAANNNNTTC 1 cut(s) 479
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.