Rroxscaffold_2G00099060

23 kDa jasmonate-induced protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001765
Physical Location & Seq
Forward (+)
20554238 .. 20554903
666 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099060.1

Sequence Viewer

Length: 549 bp
ATGGCAAACATGTTTGGCGACCCCATAAAATTGCTAGAGTCAGTCCACAAGGTCACAGTTGACAAGGCACACGAGACAATTAACATGAAAAACGCCAACATGAAGCATGCGAATGCCCGCACTTATGTGGAGAATCTGAAGAATATAGATTGGTTTGGGCACATTGGACCAGCCCCATACCCGAATATAATTCAAAATAGCCAGTGGGGTGCTTTTTTGCACATCAAAAATTCTAAAGTGCCATCTGGATCCATGGCAGGTGTTATGTATCGCAGCAAGAGTGGAAACGAAGGCAAGGATTGTGACTACATGATGGCGTGGTCAAACCCATGGCGTAGAATAGATTTGGACAACTCGGTCTGGACTGAGGCCCGTGAAGTGAACCACTTCATTGATGGAATATGGAACAACTTATCTGGAAAGTTTGATGAGACTCGCTACAACTACCGTGACATATGGGAGGGATGCTTGTCAACTATATCAACTAATAGTGATAGTTTCCCTGTGGTGGAAGTAATGTTCACTCTAGAGGATGTCGTCAATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

182

Amino Acids

20.81

Weight (kDa)

5.84

Isoelectric Point (pI)

35.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nakanori PF21230 48 - 162 9.7e-21 Nakanori-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 248
AasI GACNNNNNNGTC 1 cut(s) 356
Acc36I ACCTGC 1 cut(s) 248
AciI CCGC 1 cut(s) 118
AclWI GGATC 2 cut(s) 243, 256
AcsI RAATTY 1 cut(s) 229
AcuI CTGAAG 1 cut(s) 158
AfiI CCNNNNNNNGG 1 cut(s) 508
AflIII ACRYGT 1 cut(s) 9
AgsI TTSAA 1 cut(s) 194
AleI CACNNNNGTG 1 cut(s) 125
AloI GAACNNNNNNTCC 2 cut(s) 503, 535
Alw26I GTCTC 2 cut(s) 68, 425
AlwI GGATC 2 cut(s) 243, 256
AoxI GGCC 1 cut(s) 369
ApeKI GCWGC 1 cut(s) 273
ApoI RAATTY 1 cut(s) 229
Asp700I GAANNNNTTC 1 cut(s) 386
AspS9I GGNCC 2 cut(s) 167, 370
AvaII GGWCC 1 cut(s) 167
BaeGI GKGCMC 1 cut(s) 162
BamHI GGATCC 1 cut(s) 248
BauI CACGAG 1 cut(s) 71
BbvI GCAGC 1 cut(s) 285
BccI CCATC 3 cut(s) 250, 307, 389
BcoDI GTCTC 2 cut(s) 68, 425
BfaI CTAG 2 cut(s) 35, 527
BfuAI ACCTGC 1 cut(s) 248
BisI GCNGC 1 cut(s) 274
BlsI GCNGC 1 cut(s) 275
Bme18I GGWCC 1 cut(s) 167
BmgT120I GGNCC 2 cut(s) 167, 370
BmiI GGNNCC 1 cut(s) 250
BmsI GCATC 1 cut(s) 455
BsaJI CCNNGG 2 cut(s) 252, 329
Bsc4I CCNNNNNNNGG 1 cut(s) 508
Bse1I ACTGG 1 cut(s) 202
BseDI CCNNGG 2 cut(s) 252, 329
BseGI GGATG 2 cut(s) 470, 538
BseLI CCNNNNNNNGG 1 cut(s) 508
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 1 cut(s) 202
BseSI GKGCMC 1 cut(s) 162
BseXI GCAGC 1 cut(s) 285
BshFI GGCC 1 cut(s) 371
BslI CCNNNNNNNGG 1 cut(s) 508
BsmAI GTCTC 2 cut(s) 68, 425
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 162
Bsp143I GATC 1 cut(s) 248
Bsp19I CCATGG 2 cut(s) 252, 329
BspACI CCGC 1 cut(s) 118
BspANI GGCC 1 cut(s) 371
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 250
BspMI ACCTGC 1 cut(s) 248
BspPI GGATC 2 cut(s) 243, 256
BsrI ACTGG 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 252, 329
BssMI GATC 1 cut(s) 248
BssSI CACGAG 1 cut(s) 71
BssT1I CCWWGG 2 cut(s) 252, 329
Bst2BI CACGAG 1 cut(s) 71
Bst4CI ACNGT 2 cut(s) 58, 449
BstC8I GCNNGC 2 cut(s) 108, 118
BstDEI CTNAG 1 cut(s) 366
BstDSI CCRYGG 2 cut(s) 252, 329
BstF5I GGATG 2 cut(s) 470, 538
BstKTI GATC 1 cut(s) 251
BstMAI GTCTC 2 cut(s) 68, 425
BstMBI GATC 1 cut(s) 248
BstNSI RCATGY 2 cut(s) 13, 110
BstSLI GKGCMC 1 cut(s) 162
BstV1I GCAGC 1 cut(s) 285
BstX2I RGATCY 1 cut(s) 248
BstYI RGATCY 1 cut(s) 248
BsuRI GGCC 1 cut(s) 371
BtgI CCRYGG 2 cut(s) 252, 329
BtsCI GGATG 2 cut(s) 470, 538
BtsIMutI CAGTG 1 cut(s) 209
BveI ACCTGC 1 cut(s) 248
Cac8I GCNNGC 2 cut(s) 108, 118
Cfr13I GGNCC 2 cut(s) 167, 370
CviAII CATG 7 cut(s) 10, 85, 100, 107, 253, 310, 330
CviJI RGCY 3 cut(s) 173, 201, 371
CviKI_1 RGCY 3 cut(s) 173, 201, 371
DdeI CTNAG 1 cut(s) 366
DpnI GATC 1 cut(s) 250
DpnII GATC 1 cut(s) 248
DrdI GACNNNNNNGTC 1 cut(s) 356
DseDI GACNNNNNNGTC 1 cut(s) 356
Eco130I CCWWGG 2 cut(s) 252, 329
Eco47I GGWCC 1 cut(s) 167
Eco57I CTGAAG 1 cut(s) 158
EcoT14I CCWWGG 2 cut(s) 252, 329
ErhI CCWWGG 2 cut(s) 252, 329
FaeI CATG 7 cut(s) 13, 88, 103, 110, 256, 313, 333
FatI CATG 7 cut(s) 9, 84, 99, 106, 252, 309, 329
FauI CCCGC 1 cut(s) 125
FauNDI CATATG 1 cut(s) 455
Fnu4HI GCNGC 1 cut(s) 274
FokI GGATG 2 cut(s) 477, 545
Fsp4HI GCNGC 1 cut(s) 274
FspBI CTAG 2 cut(s) 35, 527
GluI GCNGC 1 cut(s) 274
HaeIII GGCC 1 cut(s) 371
Hin1II CATG 7 cut(s) 13, 88, 103, 110, 256, 313, 333
HincII GTYRAC 2 cut(s) 61, 474
HindII GTYRAC 2 cut(s) 61, 474
HinfI GANTC 3 cut(s) 38, 133, 433
Hpy166II GTNNAC 5 cut(s) 46, 61, 382, 474, 522
Hpy188I TCNGA 1 cut(s) 138
Hpy188III TCNNGA 4 cut(s) 246, 361, 417, 527
Hpy8I GTNNAC 5 cut(s) 46, 61, 382, 474, 522
HpyAV CCTTC 1 cut(s) 284
HpyCH4III ACNGT 2 cut(s) 58, 449
HpyCH4V TGCA 1 cut(s) 220
HpyF3I CTNAG 1 cut(s) 366
Hsp92II CATG 7 cut(s) 13, 88, 103, 110, 256, 313, 333
Kzo9I GATC 1 cut(s) 248
LpnPI CCDG 7 cut(s) 183, 215, 231, 243, 346, 402, 516
Lsp1109I GCAGC 1 cut(s) 285
LweI GCATC 1 cut(s) 455
MaeI CTAG 2 cut(s) 35, 527
MaeIII GTNAC 3 cut(s) 52, 302, 449
MalI GATC 1 cut(s) 250
MboI GATC 1 cut(s) 248
MboII GAAGA 1 cut(s) 151
MflI RGATCY 1 cut(s) 248
MhlI GDGCHC 1 cut(s) 162
MluCI AATT 4 cut(s) 29, 78, 189, 229
MlyI GAGTC 2 cut(s) 47, 427
MnlI CCTC 3 cut(s) 361, 454, 523
MroXI GAANNNNTTC 1 cut(s) 386
MseI TTAA 1 cut(s) 81
MslI CAYNNNNRTG 2 cut(s) 111, 125
Mva1269I GAATGC 1 cut(s) 118
NcoI CCATGG 2 cut(s) 252, 329
NdeI CATATG 1 cut(s) 455
NdeII GATC 1 cut(s) 248
NlaIII CATG 7 cut(s) 13, 88, 103, 110, 256, 313, 333
NlaIV GGNNCC 1 cut(s) 250
NmuCI GTSAC 3 cut(s) 52, 302, 449
NspI RCATGY 2 cut(s) 13, 110
OliI CACNNNNGTG 1 cut(s) 125
PaeI GCATGC 1 cut(s) 110
PaqCI CACCTGC 1 cut(s) 248
PciI ACATGT 1 cut(s) 9
PctI GAATGC 1 cut(s) 118
PdmI GAANNNNTTC 1 cut(s) 386
PfeI GAWTC 1 cut(s) 133
PkrI GCNGC 1 cut(s) 275
PleI GAGTC 2 cut(s) 46, 427
PpsI GAGTC 2 cut(s) 46, 427
PscI ACATGT 1 cut(s) 9
PspN4I GGNNCC 1 cut(s) 250
PspPI GGNCC 2 cut(s) 167, 370
PsuI RGATCY 1 cut(s) 248
RseI CAYNNNNRTG 2 cut(s) 111, 125
SaqAI TTAA 1 cut(s) 81
SatI GCNGC 1 cut(s) 274
Sau3AI GATC 1 cut(s) 248
Sau96I GGNCC 2 cut(s) 167, 370
SchI GAGTC 2 cut(s) 47, 427
SduI GDGCHC 1 cut(s) 162
SetI ASST 2 cut(s) 54, 262
SfaNI GCATC 1 cut(s) 455
SinI GGWCC 1 cut(s) 167
SmiMI CAYNNNNRTG 2 cut(s) 111, 125
SphI GCATGC 1 cut(s) 110
Sse9I AATT 4 cut(s) 29, 78, 189, 229
SsiI CCGC 1 cut(s) 118
SspMI CTAG 2 cut(s) 35, 527
StyI CCWWGG 2 cut(s) 252, 329
TaaI ACNGT 2 cut(s) 58, 449
TaqII GACCGA 1 cut(s) 346
TasI AATT 4 cut(s) 29, 78, 189, 229
TfiI GAWTC 1 cut(s) 133
Tru1I TTAA 1 cut(s) 81
Tru9I TTAA 1 cut(s) 81
TscAI CASTG 1 cut(s) 209
TseFI GTSAC 3 cut(s) 52, 302, 449
TseI GCWGC 1 cut(s) 273
Tsp45I GTSAC 3 cut(s) 52, 302, 449
TspDTI ATGAA 3 cut(s) 101, 116, 379
TspRI CASTG 1 cut(s) 209
VpaK11BI GGWCC 1 cut(s) 167
XapI RAATTY 1 cut(s) 229
XbaI TCTAGA 1 cut(s) 526
XceI RCATGY 2 cut(s) 13, 110
XcmI CCANNNNNNNNNTGG 1 cut(s) 392
XmnI GAANNNNTTC 1 cut(s) 386
XspI CTAG 2 cut(s) 35, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.