MD17G1148200.v1.1

23 kDa jasmonate-induced protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0001765
Physical Location & Seq
Forward (+)
13520798 .. 13521340
543 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1148200.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCAGATAATGTCTTTGGCGAACCCATCACAAACGCAACTTTGGAAGGCATGCGAGAGTATGCATGTAGGCGGGGGCAAATTGAAAGAATCAACAGGGCACGCGTGGCCATGAATATGAAAAACGCGGCCGGGAAGGACGTGAAAGCGCGAACACATGCGGAAAAATTGAAGGCAAAATCCGGTGCCGGAATCGCCACTCTCTGCCTTGTTTACAATGCGACGGGAAGCACTCTAGAATATGTTGGTCAAAAAGATTGGATTGGCCTCATGGGAAAGTCCCCATACCCACCACTTATTGCAAATGGCCAGTGGGCTGCGTTTCTGCACGTCAGAGACCCCAAAATTCAATGGGGCTCTTCTGCCGCTGTAGTGTATCGCGGAAAGAACGCTGGTGGTATCAATTGCGACTGGATGCTGAGTTGGGCCAACCCGAAGGACAGGGTTACATGGGACAACAGGGTAAGTAGTGATTTTATCTGTATATACATATTTGAAATCGAACGCATTTCATATATCTCAGAGCATATTTACTTAGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

181

Amino Acids

20.16

Weight (kDa)

9.04

Isoelectric Point (pI)

22.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nakanori PF21230 34 - 156 1.6e-33 Nakanori-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 185
AccII CGCG 4 cut(s) 105, 128, 151, 381
AciI CCGC 5 cut(s) 73, 128, 161, 366, 381
AcoI YGGCCR 3 cut(s) 108, 129, 307
AcsI RAATTY 1 cut(s) 345
AflIII ACRYGT 1 cut(s) 103
AgsI TTSAA 4 cut(s) 86, 172, 350, 497
AjiI CACGTC 2 cut(s) 142, 331
Alw26I GTCTC 1 cut(s) 330
AoxI GGCC 5 cut(s) 108, 129, 265, 307, 426
ApeKI GCWGC 1 cut(s) 317
ApoI RAATTY 1 cut(s) 345
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 426
AsuC2I CCSGG 1 cut(s) 133
BaeGI GKGCMC 1 cut(s) 103
BalI TGGCCA 2 cut(s) 110, 309
BanI GGYRCC 1 cut(s) 185
BanII GRGCYC 1 cut(s) 359
BbvI GCAGC 1 cut(s) 304
BccI CCATC 1 cut(s) 35
BcnI CCSGG 1 cut(s) 133
BcoDI GTCTC 1 cut(s) 330
BfaI CTAG 1 cut(s) 236
BfmI CTRYAG 1 cut(s) 369
BisI GCNGC 3 cut(s) 129, 318, 366
BlsI GCNGC 3 cut(s) 130, 319, 367
Bme1390I CCNGG 1 cut(s) 133
BmgBI CACGTC 2 cut(s) 142, 331
BmgT120I GGNCC 1 cut(s) 426
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 1 cut(s) 133
BmsI GCATC 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 330
BsaWI WCCGGW 1 cut(s) 182
Bse1I ACTGG 2 cut(s) 310, 416
BseGI GGATG 1 cut(s) 420
BseMII CTCAG 2 cut(s) 410, 534
BseNI ACTGG 2 cut(s) 310, 416
BseSI GKGCMC 1 cut(s) 103
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 1 cut(s) 304
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 4 cut(s) 105, 128, 151, 381
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 5 cut(s) 110, 131, 267, 309, 428
BshNI GGYRCC 1 cut(s) 185
BsiEI CGRYCG 1 cut(s) 132
BsiSI CCGG 3 cut(s) 132, 183, 189
BslFI GGGAC 2 cut(s) 265, 467
BsmAI GTCTC 1 cut(s) 330
BsmFI GGGAC 2 cut(s) 265, 467
BsnI GGCC 5 cut(s) 110, 131, 267, 309, 428
Bso31I GGTCTC 1 cut(s) 330
Bsp1286I GDGCHC 2 cut(s) 103, 359
BspACI CCGC 5 cut(s) 73, 128, 161, 366, 381
BspANI GGCC 5 cut(s) 110, 131, 267, 309, 428
BspCNI CTCAG 2 cut(s) 411, 533
BspFNI CGCG 4 cut(s) 105, 128, 151, 381
BspLI GGNNCC 1 cut(s) 187
BspQI GCTCTTC 1 cut(s) 364
BspT107I GGYRCC 1 cut(s) 185
BspTNI GGTCTC 1 cut(s) 330
BsrI ACTGG 2 cut(s) 310, 416
Bst6I CTCTTC 1 cut(s) 364
BstC8I GCNNGC 2 cut(s) 53, 103
BstDEI CTNAG 3 cut(s) 419, 520, 535
BstF5I GGATG 1 cut(s) 420
BstFNI CGCG 4 cut(s) 105, 128, 151, 381
BstHHI GCGC 1 cut(s) 151
BstMAI GTCTC 1 cut(s) 330
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 107, 194
BstNSI RCATGY 3 cut(s) 55, 69, 161
BstSCI CCNGG 1 cut(s) 131
BstSFI CTRYAG 1 cut(s) 369
BstSLI GKGCMC 1 cut(s) 103
BstUI CGCG 4 cut(s) 105, 128, 151, 381
BstV1I GCAGC 1 cut(s) 304
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 5 cut(s) 110, 131, 267, 309, 428
BtrI CACGTC 2 cut(s) 142, 331
BtsCI GGATG 1 cut(s) 420
BtsIMutI CAGTG 1 cut(s) 317
Cac8I GCNNGC 2 cut(s) 53, 103
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 426
CviAII CATG 6 cut(s) 52, 66, 112, 158, 271, 450
CviJI RGCY 7 cut(s) 110, 131, 267, 309, 317, 357, 428
CviKI_1 RGCY 7 cut(s) 110, 131, 267, 309, 317, 357, 428
DdeI CTNAG 3 cut(s) 419, 520, 535
EaeI YGGCCR 3 cut(s) 108, 129, 307
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 1 cut(s) 364
EarI CTCTTC 1 cut(s) 364
EclXI CGGCCG 1 cut(s) 129
Eco24I GRGCYC 1 cut(s) 359
Eco31I GGTCTC 1 cut(s) 330
Eco52I CGGCCG 1 cut(s) 129
EcoT22I ATGCAT 1 cut(s) 67
EcoT38I GRGCYC 1 cut(s) 359
FaeI CATG 6 cut(s) 55, 69, 115, 161, 274, 453
FaqI GGGAC 2 cut(s) 265, 467
FatI CATG 6 cut(s) 51, 65, 111, 157, 270, 449
FauI CCCGC 1 cut(s) 66
Fnu4HI GCNGC 3 cut(s) 129, 318, 366
FokI GGATG 1 cut(s) 427
FriOI GRGCYC 1 cut(s) 359
Fsp4HI GCNGC 3 cut(s) 129, 318, 366
FspBI CTAG 1 cut(s) 236
GlaI GCGC 1 cut(s) 150
GluI GCNGC 3 cut(s) 129, 318, 366
HaeIII GGCC 5 cut(s) 110, 131, 267, 309, 428
HapII CCGG 3 cut(s) 132, 183, 189
HhaI GCGC 1 cut(s) 151
Hin1II CATG 6 cut(s) 55, 69, 115, 161, 274, 453
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 2 cut(s) 90, 192
HpaII CCGG 3 cut(s) 132, 183, 189
Hpy166II GTNNAC 1 cut(s) 214
Hpy188I TCNGA 2 cut(s) 335, 523
Hpy188III TCNNGA 1 cut(s) 236
Hpy8I GTNNAC 1 cut(s) 214
Hpy99I CGWCG 1 cut(s) 226
HpyAV CCTTC 4 cut(s) 41, 130, 166, 430
HpyCH4IV ACGT 2 cut(s) 141, 330
HpyCH4V TGCA 3 cut(s) 65, 302, 328
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 194
HpyF3I CTNAG 3 cut(s) 419, 520, 535
HpySE526I ACGT 2 cut(s) 141, 330
Hsp92II CATG 6 cut(s) 55, 69, 115, 161, 274, 453
HspAI GCGC 1 cut(s) 149
LguI GCTCTTC 1 cut(s) 364
LpnPI CCDG 9 cut(s) 82, 145, 196, 202, 323, 378, 397, 427, 445
Lsp1109I GCAGC 1 cut(s) 304
LweI GCATC 1 cut(s) 405
MaeI CTAG 1 cut(s) 236
MaeII ACGT 2 cut(s) 141, 330
MaeIII GTNAC 1 cut(s) 445
MboII GAAGA 1 cut(s) 351
MfeI CAATTG 1 cut(s) 403
MhlI GDGCHC 2 cut(s) 103, 359
MlsI TGGCCA 2 cut(s) 110, 309
MluCI AATT 4 cut(s) 81, 167, 345, 403
MluI ACGCGT 1 cut(s) 103
MluNI TGGCCA 2 cut(s) 110, 309
MnlI CCTC 1 cut(s) 278
Mox20I TGGCCA 2 cut(s) 110, 309
Mph1103I ATGCAT 1 cut(s) 67
MscI TGGCCA 2 cut(s) 110, 309
MslI CAYNNNNRTG 1 cut(s) 116
Msp20I TGGCCA 2 cut(s) 110, 309
MspA1I CMGCKG 1 cut(s) 368
MspI CCGG 3 cut(s) 132, 183, 189
MspR9I CCNGG 1 cut(s) 133
MunI CAATTG 1 cut(s) 403
MvnI CGCG 4 cut(s) 105, 128, 151, 381
MwoI GCNNNNNNNGC 2 cut(s) 107, 194
NciI CCSGG 1 cut(s) 133
NlaIII CATG 6 cut(s) 55, 69, 115, 161, 274, 453
NlaIV GGNNCC 1 cut(s) 187
NsiI ATGCAT 1 cut(s) 67
NspI RCATGY 3 cut(s) 55, 69, 161
PaeI GCATGC 1 cut(s) 55
PciSI GCTCTTC 1 cut(s) 364
PfeI GAWTC 2 cut(s) 90, 192
PkrI GCNGC 3 cut(s) 130, 319, 367
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 1 cut(s) 426
RseI CAYNNNNRTG 1 cut(s) 116
SapI GCTCTTC 1 cut(s) 364
SatI GCNGC 3 cut(s) 129, 318, 366
Sau96I GGNCC 1 cut(s) 426
ScrFI CCNGG 1 cut(s) 133
SduI GDGCHC 2 cut(s) 103, 359
SetI ASST 2 cut(s) 144, 333
SfaNI GCATC 1 cut(s) 405
SfcI CTRYAG 1 cut(s) 369
SmiMI CAYNNNNRTG 1 cut(s) 116
SphI GCATGC 1 cut(s) 55
Sse9I AATT 4 cut(s) 81, 167, 345, 403
SsiI CCGC 5 cut(s) 73, 128, 161, 366, 381
SspMI CTAG 1 cut(s) 236
StyD4I CCNGG 1 cut(s) 131
TaiI ACGT 2 cut(s) 144, 333
TaqI TCGA 1 cut(s) 501
TasI AATT 4 cut(s) 81, 167, 345, 403
TauI GCSGC 2 cut(s) 131, 368
TfiI GAWTC 2 cut(s) 90, 192
TscAI CASTG 1 cut(s) 317
TseI GCWGC 1 cut(s) 317
TspDTI ATGAA 3 cut(s) 128, 134, 501
TspRI CASTG 1 cut(s) 317
XapI RAATTY 1 cut(s) 345
XbaI TCTAGA 1 cut(s) 235
XceI RCATGY 3 cut(s) 55, 69, 161
XspI CTAG 1 cut(s) 236
Zsp2I ATGCAT 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.