Rroxscaffold_2G00099100

23 kDa jasmonate-induced protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001765
Physical Location & Seq
Forward (+)
20604591 .. 20605749
1159 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00099100.1

Sequence Viewer

Length: 465 bp
ATGCTACCGGGGCACACTATGTCGTTGATTGGCAATAATAAAAATTGGGAGGGGCACATTGGACAAGCCCCATACCCAATAGAAATTCAAAATGGCCAGTGGGGTGCATTTTTGCACGTCGAAGAATATAGAAAGAAGTCTGGATCTGCAGCAGGTGTTGTGTATCGCGGCAAGAACGGAAATGAAGTTGAGTGTGACCACATGATGGCGTGGTCCAACCCACGGCCTGAAAAATGTACAAAGAACTCGGTCTGGGTTGAGACCCGTGGAGCTGGCCACTACGATGATCCCACATGGAACCACATACATGTAATGCTTGCTAAGGCTCGCGACAGCTACAATGATGGCCCGGCATGGGAGGGATGCTCGTCAAAAATAACAACTGGTAATGTGACCACTGCTAATGACATTTTCGTCGAACTGGAAGCAACATTCACTTTAAAAGATGCCGAGGACGATGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

154

Amino Acids

17.12

Weight (kDa)

5.42

Isoelectric Point (pI)

35.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Nakanori PF21230 12 - 129 1.8e-21 Nakanori-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 143
AasI GACNNNNNNGTC 1 cut(s) 413
Acc36I ACCTGC 1 cut(s) 143
AccB7I CCANNNNNTGG 1 cut(s) 205
AccII CGCG 2 cut(s) 168, 330
AciI CCGC 1 cut(s) 168
AclWI GGATC 2 cut(s) 151, 281
AcoI YGGCCR 2 cut(s) 94, 274
AcsI RAATTY 1 cut(s) 84
AfaI GTAC 1 cut(s) 238
AfiI CCNNNNNNNGG 3 cut(s) 205, 222, 355
AflIII ACRYGT 1 cut(s) 307
AgsI TTSAA 1 cut(s) 89
AjiI CACGTC 1 cut(s) 118
AluBI AGCT 2 cut(s) 272, 336
AluI AGCT 2 cut(s) 272, 336
Alw26I GTCTC 1 cut(s) 254
AlwI GGATC 2 cut(s) 151, 281
AoxI GGCC 4 cut(s) 94, 224, 274, 346
ApeKI GCWGC 1 cut(s) 149
ApoI RAATTY 1 cut(s) 84
AspS9I GGNCC 2 cut(s) 213, 347
AsuC2I CCSGG 2 cut(s) 9, 350
AvaII GGWCC 1 cut(s) 213
BaeGI GKGCMC 2 cut(s) 15, 57
BalI TGGCCA 2 cut(s) 96, 276
BbvI GCAGC 1 cut(s) 161
BccI CCATC 2 cut(s) 199, 338
BceAI ACGGC 1 cut(s) 239
BcgI CGANNNNNNTGC 1 cut(s) 440
BcnI CCSGG 2 cut(s) 9, 350
BcoDI GTCTC 1 cut(s) 254
BfmI CTRYAG 1 cut(s) 147
BfuAI ACCTGC 1 cut(s) 143
BisI GCNGC 2 cut(s) 150, 169
BlsI GCNGC 2 cut(s) 151, 170
Bme1390I CCNGG 2 cut(s) 9, 350
Bme18I GGWCC 1 cut(s) 213
BmgBI CACGTC 1 cut(s) 118
BmgT120I GGNCC 2 cut(s) 213, 347
BmiI GGNNCC 1 cut(s) 299
BmrFI CCNGG 2 cut(s) 9, 350
BmsI GCATC 3 cut(s) 353, 436, 448
BplI GAGNNNNNCTC 2 cut(s) 350, 382
Bpu10I CCTNAGC 1 cut(s) 321
BpuMI CCSGG 2 cut(s) 9, 350
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 4 cut(s) 8, 221, 265, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 205, 222, 355
Bse1I ACTGG 3 cut(s) 97, 388, 426
BseDI CCNNGG 4 cut(s) 8, 221, 265, 450
BseGI GGATG 1 cut(s) 368
BseLI CCNNNNNNNGG 3 cut(s) 205, 222, 355
BseNI ACTGG 3 cut(s) 97, 388, 426
BseSI GKGCMC 2 cut(s) 15, 57
BseXI GCAGC 1 cut(s) 161
Bsh1236I CGCG 2 cut(s) 168, 330
BshFI GGCC 4 cut(s) 96, 226, 276, 348
BsiSI CCGG 2 cut(s) 8, 350
BslI CCNNNNNNNGG 3 cut(s) 205, 222, 355
BsmAI GTCTC 1 cut(s) 254
BsnI GGCC 4 cut(s) 96, 226, 276, 348
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 15, 57
Bsp1407I TGTACA 1 cut(s) 236
Bsp143I GATC 2 cut(s) 143, 286
Bsp68I TCGCGA 1 cut(s) 330
BspACI CCGC 1 cut(s) 168
BspANI GGCC 4 cut(s) 96, 226, 276, 348
BspFNI CGCG 2 cut(s) 168, 330
BspLI GGNNCC 1 cut(s) 299
BspMAI CTGCAG 1 cut(s) 151
BspMI ACCTGC 1 cut(s) 143
BspPI GGATC 2 cut(s) 151, 281
BspTNI GGTCTC 1 cut(s) 254
BsrGI TGTACA 1 cut(s) 236
BsrI ACTGG 3 cut(s) 97, 388, 426
BssECI CCNNGG 4 cut(s) 8, 221, 265, 450
BssMI GATC 2 cut(s) 143, 286
BstAUI TGTACA 1 cut(s) 236
BstC8I GCNNGC 3 cut(s) 274, 318, 328
BstDEI CTNAG 1 cut(s) 321
BstDSI CCRYGG 2 cut(s) 221, 265
BstF5I GGATG 1 cut(s) 368
BstFNI CGCG 2 cut(s) 168, 330
BstKTI GATC 2 cut(s) 146, 289
BstMAI GTCTC 1 cut(s) 254
BstMBI GATC 2 cut(s) 143, 286
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNSI RCATGY 1 cut(s) 311
BstSCI CCNGG 2 cut(s) 7, 348
BstSFI CTRYAG 1 cut(s) 147
BstSLI GKGCMC 2 cut(s) 15, 57
BstUI CGCG 2 cut(s) 168, 330
BstV1I GCAGC 1 cut(s) 161
BstX2I RGATCY 1 cut(s) 143
BstYI RGATCY 1 cut(s) 143
BsuRI GGCC 4 cut(s) 96, 226, 276, 348
BtgI CCRYGG 2 cut(s) 221, 265
BtrI CACGTC 1 cut(s) 118
BtsCI GGATG 1 cut(s) 368
BtsI GCAGTG 1 cut(s) 396
BtsIMutI CAGTG 2 cut(s) 104, 396
BtuMI TCGCGA 1 cut(s) 330
BveI ACCTGC 1 cut(s) 143
Cac8I GCNNGC 3 cut(s) 274, 318, 328
Cfr13I GGNCC 2 cut(s) 213, 347
Csp6I GTAC 1 cut(s) 237
CviAII CATG 4 cut(s) 202, 294, 308, 354
CviJI RGCY 8 cut(s) 68, 96, 226, 272, 276, 326, 336, 348
CviKI_1 RGCY 8 cut(s) 68, 96, 226, 272, 276, 326, 336, 348
CviQI GTAC 1 cut(s) 237
DdeI CTNAG 1 cut(s) 321
DpnI GATC 2 cut(s) 145, 288
DpnII GATC 2 cut(s) 143, 286
DraI TTTAAA 1 cut(s) 441
DrdI GACNNNNNNGTC 1 cut(s) 413
DseDI GACNNNNNNGTC 1 cut(s) 413
EaeI YGGCCR 2 cut(s) 94, 274
Eco31I GGTCTC 1 cut(s) 254
Eco47I GGWCC 1 cut(s) 213
FaeI CATG 4 cut(s) 205, 297, 311, 357
FaiI YATR 8 cut(s) 20, 73, 129, 203, 295, 305, 309, 355
FatI CATG 4 cut(s) 201, 293, 307, 353
Fnu4HI GCNGC 2 cut(s) 150, 169
FokI GGATG 1 cut(s) 375
Fsp4HI GCNGC 2 cut(s) 150, 169
GluI GCNGC 2 cut(s) 150, 169
HaeIII GGCC 4 cut(s) 96, 226, 276, 348
HapII CCGG 2 cut(s) 8, 350
Hin1II CATG 4 cut(s) 205, 297, 311, 357
HpaII CCGG 2 cut(s) 8, 350
Hpy188III TCNNGA 2 cut(s) 141, 329
Hpy99I CGWCG 2 cut(s) 122, 419
HpyCH4IV ACGT 1 cut(s) 117
HpyCH4V TGCA 3 cut(s) 107, 115, 149
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 321
HpySE526I ACGT 1 cut(s) 117
Hsp92II CATG 4 cut(s) 205, 297, 311, 357
Kzo9I GATC 2 cut(s) 143, 286
LmnI GCTCC 1 cut(s) 269
Lsp1109I GCAGC 1 cut(s) 161
LweI GCATC 3 cut(s) 353, 436, 448
MaeII ACGT 1 cut(s) 117
MaeIII GTNAC 2 cut(s) 194, 391
MalI GATC 2 cut(s) 145, 288
MboI GATC 2 cut(s) 143, 286
MboII GAAGA 1 cut(s) 134
MflI RGATCY 1 cut(s) 143
MhlI GDGCHC 2 cut(s) 15, 57
MlsI TGGCCA 2 cut(s) 96, 276
MluCI AATT 2 cut(s) 43, 84
MluNI TGGCCA 2 cut(s) 96, 276
MmeI TCCRAC 1 cut(s) 240
MnlI CCTC 3 cut(s) 43, 352, 445
Mox20I TGGCCA 2 cut(s) 96, 276
MscI TGGCCA 2 cut(s) 96, 276
MseI TTAA 1 cut(s) 440
MslI CAYNNNNRTG 2 cut(s) 282, 306
Msp20I TGGCCA 2 cut(s) 96, 276
MspI CCGG 2 cut(s) 8, 350
MspR9I CCNGG 2 cut(s) 9, 350
MvnI CGCG 2 cut(s) 168, 330
MwoI GCNNNNNNNGC 1 cut(s) 10
NciI CCSGG 2 cut(s) 9, 350
NdeII GATC 2 cut(s) 143, 286
NlaIII CATG 4 cut(s) 205, 297, 311, 357
NlaIV GGNNCC 1 cut(s) 299
NmuCI GTSAC 2 cut(s) 194, 391
NruI TCGCGA 1 cut(s) 330
NspI RCATGY 1 cut(s) 311
PaqCI CACCTGC 1 cut(s) 143
PciI ACATGT 1 cut(s) 307
PflMI CCANNNNNTGG 1 cut(s) 205
PkrI GCNGC 2 cut(s) 151, 170
PscI ACATGT 1 cut(s) 307
PspN4I GGNNCC 1 cut(s) 299
PspPI GGNCC 2 cut(s) 213, 347
PstI CTGCAG 1 cut(s) 151
PsuI RGATCY 1 cut(s) 143
RruI TCGCGA 1 cut(s) 330
RsaI GTAC 1 cut(s) 238
RsaNI GTAC 1 cut(s) 237
RseI CAYNNNNRTG 2 cut(s) 282, 306
SaqAI TTAA 1 cut(s) 440
SatI GCNGC 2 cut(s) 150, 169
Sau3AI GATC 2 cut(s) 143, 286
Sau96I GGNCC 2 cut(s) 213, 347
ScrFI CCNGG 2 cut(s) 9, 350
SduI GDGCHC 2 cut(s) 15, 57
SetI ASST 4 cut(s) 120, 157, 274, 338
SfaNI GCATC 3 cut(s) 353, 436, 448
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 1 cut(s) 213
SmiMI CAYNNNNRTG 2 cut(s) 282, 306
Sse9I AATT 2 cut(s) 43, 84
SsiI CCGC 1 cut(s) 168
StyD4I CCNGG 2 cut(s) 7, 348
TaiI ACGT 1 cut(s) 120
TaqI TCGA 2 cut(s) 120, 417
TaqII GACCGA 1 cut(s) 238
TasI AATT 2 cut(s) 43, 84
TatI WGTACW 1 cut(s) 236
TauI GCSGC 1 cut(s) 171
Tru1I TTAA 1 cut(s) 440
Tru9I TTAA 1 cut(s) 440
TscAI CASTG 2 cut(s) 104, 403
TseFI GTSAC 2 cut(s) 194, 391
TseI GCWGC 1 cut(s) 149
Tsp45I GTSAC 2 cut(s) 194, 391
TspDTI ATGAA 1 cut(s) 198
TspGWI ACGGA 1 cut(s) 192
TspRI CASTG 2 cut(s) 104, 403
Van91I CCANNNNNTGG 1 cut(s) 205
VpaK11BI GGWCC 1 cut(s) 213
XapI RAATTY 1 cut(s) 84
XceI RCATGY 1 cut(s) 311
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.