MD10G1088200.v1.1

Belongs to the iron ascorbate-dependent oxidoreductase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000590
Physical Location & Seq
Forward (+)
13057763 .. 13058473
711 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1088200.v1.1.491

Sequence Viewer

Length: 591 bp
ATGGAGTACAACTGGATACGCTCGGAAAGACTGAGGCTGCTGCTCGGAAGTTTTTTTGATCTGCCTTCGGAGGAGAAAAGGCGAATTTGGAGGGATGAAAAAACTGTGTTGGGTTACTACGATACTGAGCATACCAAGAACGTACGAGACTGGAAGGAGGTGTTCGATTTCACAGTGGAGAAACCTATACACAAATTTGGAATTGGACTCGTAAGATCTAATACCAGGGTGCTGTGTGATGAATACGCTCGAGAAGTAGAAAAACTAGCTCTCAAGTTGTTGGGACTTATTGCCTTGAGCCTAGGCTTGCCAGAAAACAGGTTCAACAGCTACTTCGAAGACCGGACAAGCTTTATCAGGCTCAATCACTATCCACCTTGCCCTTCCCCTCAGTTAGCTATTGGTGTTGGTCGCCACAAGGACAGCGGTGCTCTAACCGTGCTGTCTCAGGATGATGTCGGAGGACTGGAGGTGATGAGAAAAGCAGACGGAGAGTGGATAACCCCAAATGCCTATATCATCAATGTTGGTGACATTCTTCAGGATCCTCATCCAAGCAATACAAACCAAAACCTAAAAGCTACTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
2OG-FeII_Oxy DIOX_N

Protein Analysis

197

Amino Acids

22.62

Weight (kDa)

6.22

Isoelectric Point (pI)

44.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 12 - 62 2.2e-08 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 117 - 181 1.7e-15 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000590)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G19000 AT3G19000
fragaria_vesca FvH4_2g07071 FvH4_2g07080 FvH4_2g07080 FvH4_2g07081 FvH4_2g07082 FvH4_2g07082
malus_domestica MD05G1074000.v1.1 MD10G1088100.v1.1 MD10G1088200.v1.1 MD14G1207600.v1.1
prunus_persica Prupe.8G114100_v2.0.a1 Prupe.8G114200_v2.0.a1 Prupe.8G114300_v2.0.a1 Prupe.8G114500_v2.0.a1 Prupe.8G114800_v2.0.a1 Prupe.8G115100_v2.0.a1
pyrus_communis pycom05g06640 pycom05g06660 pycom05g06670 pycom10g07050
rosa_chinensis RchiOBHm_Chr6g0244141 RchiOBHm_Chr6g0259511 RchiOBHm_Chr6g0259521 RchiOBHm_Chr6g0259531 RchiOBHm_Chr6g0259551 RchiOBHm_Chr6g0259561
rosa_laevigata RLG00000014522 RLG00000014523 RLG00000014524 RLG00000014525 RLG00000014526 RLG00000014527
rosa_multiflora Rmu_ssc0000050.1_g000019 Rmu_ssc0000050.1_g000020 Rmu_ssc0000050.1_g000026 Rmu_ssc0000050.1_g000027 Rmu_ssc0000050.1_g000028
rosa_roxburghii Rroxscaffold_7G00206780 Rroxscaffold_7G00206790 Rroxscaffold_7G00206850 Rroxscaffold_7G00206860 Rroxscaffold_7G00206870 Rroxscaffold_7G00206880
rosa_rugosa Rorug05G0589100 Rorug05G0589200.1 Rorug05G0589300 Rorug05G0589400 Rorug05G0589500 Rorug05G0589600
rosa_samantha Rh4CG096000 Rh5BG231700 Rh6AG104900 Rh6AG105000 Rh6AG105100 Rh6AG105500 Rh6AG105600 Rh6BG098500 Rh6BG098700 Rh6BG098800 Rh6BG099000 Rh6BG099100 Rh6CG094200 Rh6CG094300 Rh6CG094400 Rh6CG094500 Rh6CG094600 Rh6DG088300 Rh6DG088400 Rh6DG088500 Rh6DG088600 Rh6DG088700
rosa_wichuraiana Rw0G014270 Rw0G014280 Rw0G014290 Rw6G009080 Rw6G009090 Rw6G009100 Rw6G009110 Rw6G009120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 426
AclWI GGATC 2 cut(s) 539, 552
AcsI RAATTY 2 cut(s) 84, 194
AcuI CTGAAG 1 cut(s) 524
AfaI GTAC 2 cut(s) 8, 144
AgsI TTSAA 1 cut(s) 325
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 5 cut(s) 269, 330, 351, 398, 581
AluI AGCT 5 cut(s) 269, 330, 351, 398, 581
Alw21I GWGCWC 1 cut(s) 433
Alw26I GTCTC 2 cut(s) 141, 450
AlwI GGATC 2 cut(s) 539, 552
Ama87I CYCGRG 1 cut(s) 249
ApeKI GCWGC 2 cut(s) 37, 40
ApoI RAATTY 2 cut(s) 84, 194
AspA2I CCTAGG 1 cut(s) 301
AsuHPI GGTGA 2 cut(s) 484, 542
AsuII TTCGAA 1 cut(s) 336
AvaI CYCGRG 1 cut(s) 249
AvrII CCTAGG 1 cut(s) 301
BamHI GGATCC 1 cut(s) 544
BbsI GAAGAC 1 cut(s) 345
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 2 cut(s) 24, 27
BciT130I CCWGG 1 cut(s) 226
BciVI GTATCC 1 cut(s) 9
BcoDI GTCTC 2 cut(s) 141, 450
BfaI CTAG 3 cut(s) 266, 302, 589
BfuI GTATCC 1 cut(s) 9
BglII AGATCT 1 cut(s) 215
BisI GCNGC 2 cut(s) 38, 41
BlnI CCTAGG 1 cut(s) 301
BlsI GCNGC 2 cut(s) 39, 42
Bme1390I CCNGG 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 546
BmrFI CCNGG 1 cut(s) 226
BpiI GAAGAC 1 cut(s) 345
BpmI CTGGAG 1 cut(s) 488
Bpu14I TTCGAA 1 cut(s) 336
BpuEI CTTGAG 2 cut(s) 257, 316
BsaBI GATNNNNATC 1 cut(s) 549
BsaJI CCNNGG 2 cut(s) 225, 301
BsaWI WCCGGW 1 cut(s) 342
Bse1I ACTGG 3 cut(s) 17, 155, 471
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 2 cut(s) 225, 301
BseGI GGATG 3 cut(s) 100, 457, 550
BseJI GATNNNNATC 1 cut(s) 549
BseMII CTCAG 4 cut(s) 23, 117, 404, 461
BseNI ACTGG 3 cut(s) 17, 155, 471
BseRI GAGGAG 1 cut(s) 86
BseXI GCAGC 2 cut(s) 24, 27
BsiHKAI GWGCWC 1 cut(s) 433
BsiHKCI CYCGRG 1 cut(s) 249
BsiSI CCGG 1 cut(s) 343
BsiWI CGTACG 1 cut(s) 142
BslFI GGGAC 1 cut(s) 297
BsmAI GTCTC 2 cut(s) 141, 450
BsmFI GGGAC 1 cut(s) 297
BsoBI CYCGRG 1 cut(s) 249
Bsp119I TTCGAA 1 cut(s) 336
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 3 cut(s) 58, 215, 544
BspACI CCGC 1 cut(s) 426
BspCNI CTCAG 4 cut(s) 24, 118, 403, 460
BspLI GGNNCC 1 cut(s) 546
BspPI GGATC 2 cut(s) 539, 552
BspT104I TTCGAA 1 cut(s) 336
BsrI ACTGG 3 cut(s) 17, 155, 471
BssECI CCNNGG 2 cut(s) 225, 301
BssMI GATC 3 cut(s) 58, 215, 544
BssT1I CCWWGG 1 cut(s) 301
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 3 cut(s) 106, 175, 439
BstBI TTCGAA 1 cut(s) 336
BstC8I GCNNGC 1 cut(s) 308
BstDEI CTNAG 4 cut(s) 32, 126, 390, 447
BstF5I GGATG 3 cut(s) 100, 457, 550
BstKTI GATC 3 cut(s) 61, 218, 547
BstMAI GTCTC 2 cut(s) 141, 450
BstMBI GATC 3 cut(s) 58, 215, 544
BstNI CCWGG 1 cut(s) 226
BstSCI CCNGG 1 cut(s) 224
BstV1I GCAGC 2 cut(s) 24, 27
BstV2I GAAGAC 1 cut(s) 345
BstX2I RGATCY 2 cut(s) 215, 544
BstYI RGATCY 2 cut(s) 215, 544
BsuI GTATCC 1 cut(s) 9
BtsCI GGATG 3 cut(s) 100, 457, 550
BtsIMutI CAGTG 1 cut(s) 180
Cac8I GCNNGC 1 cut(s) 308
Csp6I GTAC 2 cut(s) 7, 143
CviJI RGCY 9 cut(s) 37, 269, 300, 306, 330, 351, 361, 398, 581
CviKI_1 RGCY 9 cut(s) 37, 269, 300, 306, 330, 351, 361, 398, 581
CviQI GTAC 2 cut(s) 7, 143
DdeI CTNAG 4 cut(s) 32, 126, 390, 447
DpnI GATC 3 cut(s) 60, 217, 546
DpnII GATC 3 cut(s) 58, 215, 544
Eco130I CCWWGG 1 cut(s) 301
Eco57I CTGAAG 1 cut(s) 524
Eco88I CYCGRG 1 cut(s) 249
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 301
ErhI CCWWGG 1 cut(s) 301
FaiI YATR 3 cut(s) 132, 188, 516
FaqI GGGAC 1 cut(s) 297
Fnu4HI GCNGC 2 cut(s) 38, 41
FokI GGATG 3 cut(s) 107, 464, 537
Fsp4HI GCNGC 2 cut(s) 38, 41
FspBI CTAG 3 cut(s) 266, 302, 589
GluI GCNGC 2 cut(s) 38, 41
GsuI CTGGAG 1 cut(s) 488
HapII CCGG 1 cut(s) 343
HindIII AAGCTT 1 cut(s) 349
HinfI GANTC 1 cut(s) 207
HpaII CCGG 1 cut(s) 343
HphI GGTGA 2 cut(s) 484, 542
Hpy188I TCNGA 4 cut(s) 25, 47, 70, 461
Hpy188III TCNNGA 3 cut(s) 251, 449, 542
HpyAV CCTTC 3 cut(s) 75, 148, 393
HpyCH4III ACNGT 3 cut(s) 106, 175, 439
HpyCH4IV ACGT 1 cut(s) 141
HpyF3I CTNAG 4 cut(s) 32, 126, 390, 447
HpySE526I ACGT 1 cut(s) 141
Kzo9I GATC 3 cut(s) 58, 215, 544
Lsp1109I GCAGC 2 cut(s) 24, 27
MaeI CTAG 3 cut(s) 266, 302, 589
MaeII ACGT 1 cut(s) 141
MaeIII GTNAC 2 cut(s) 113, 530
MalI GATC 3 cut(s) 60, 217, 546
MboI GATC 3 cut(s) 58, 215, 544
MboII GAAGA 2 cut(s) 350, 530
MflI RGATCY 2 cut(s) 215, 544
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 3 cut(s) 84, 194, 201
MlyI GAGTC 1 cut(s) 201
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 8 cut(s) 27, 64, 84, 151, 399, 455, 463, 558
MspA1I CMGCKG 1 cut(s) 426
MspI CCGG 1 cut(s) 343
MspR9I CCNGG 1 cut(s) 226
MvaI CCWGG 1 cut(s) 226
NdeII GATC 3 cut(s) 58, 215, 544
NlaIV GGNNCC 1 cut(s) 546
NmuCI GTSAC 1 cut(s) 530
NspV TTCGAA 1 cut(s) 336
PaeR7I CTCGAG 1 cut(s) 249
Pfl23II CGTACG 1 cut(s) 142
PkrI GCNGC 2 cut(s) 39, 42
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PspLI CGTACG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 546
PsuI RGATCY 2 cut(s) 215, 544
RsaI GTAC 2 cut(s) 8, 144
RsaNI GTAC 2 cut(s) 7, 143
SatI GCNGC 2 cut(s) 38, 41
Sau3AI GATC 3 cut(s) 58, 215, 544
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 1 cut(s) 226
SduI GDGCHC 1 cut(s) 433
Sfr274I CTCGAG 1 cut(s) 249
SfuI TTCGAA 1 cut(s) 336
SlaI CTCGAG 1 cut(s) 249
SmlI CTYRAG 3 cut(s) 249, 272, 295
SmoI CTYRAG 3 cut(s) 249, 272, 295
Sse9I AATT 3 cut(s) 84, 194, 201
SsiI CCGC 1 cut(s) 426
SspMI CTAG 3 cut(s) 266, 302, 589
StyD4I CCNGG 1 cut(s) 224
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 3 cut(s) 106, 175, 439
TaiI ACGT 1 cut(s) 144
TaqI TCGA 3 cut(s) 165, 250, 336
TasI AATT 3 cut(s) 84, 194, 201
TatI WGTACW 1 cut(s) 6
TscAI CASTG 1 cut(s) 180
TseFI GTSAC 1 cut(s) 530
TseI GCWGC 2 cut(s) 37, 40
Tsp45I GTSAC 1 cut(s) 530
TspDTI ATGAA 2 cut(s) 111, 255
TspGWI ACGGA 1 cut(s) 504
TspRI CASTG 1 cut(s) 180
XapI RAATTY 2 cut(s) 84, 194
XhoI CTCGAG 1 cut(s) 249
XmaJI CCTAGG 1 cut(s) 301
XspI CTAG 3 cut(s) 266, 302, 589
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.